Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,428,065 A→T 6.7% L13Q (CTG→CAG)  rhlE‑I ← ATP‑dependent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,428,0650AT 6.7% 225.4 / 3.2 74L13Q (CTG→CAG) rhlE‑IATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base A (33/36);  new base T (3/2);  total (36/38)
Fisher's exact test for biased strand distribution p-value = 6.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGTCTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTT‑GGCCTGCACCGGGGTCGGGGT‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGAAGG‑CGGCAAGTTTACCGCATCGCCCCTGCCTACTTGCAGATTTCACCTTTCAACGGTTGTACAGGTTCAGGCGCCTTGCGC  >  NC_002947/5427923‑5428209
                                                                                                                                                |                                                                                                                                                 
cagcaCAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGTCTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTT‑GGCCTGCACCGGGGTCGGGGT‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCg                                                                                                                                             >  1:379334/1‑149 (MQ=255)
 agcaCAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGTCTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTT‑GGCCTGCACCGGGGTCGGGGT‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCgg                                                                                                                                            >  2:165409/1‑149 (MQ=255)
  gcaCAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGTCTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTT‑GGCCTGCACCGGGGTCGGGGT‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGt                                                                                                                                           <  1:96086/149‑1 (MQ=255)
   cacaGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGTCTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTT‑GGCCTGCACCGGGGTCGGGGG‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGtt                                                                                                                                          <  5:185458/149‑1 (MQ=255)
    acaGCCTGGGCAACGCCGGCGGTCTGGCGGTTACCGTGCGGGGCTGGGGCAATCAGGTCGCGGCCGGCCAGCAGGGCGGGCATGGCTTTCGGCCTGCACCGGGGTCGGGGT‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGtt                                                                                                                                          <  1:297695/149‑1 (MQ=255)
     caggcaggGCAAAGCCTGCGGTCTTGCCGGTACCGGTCTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTT‑GGCCTGCACCGGGGTCGGGGT‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCg                                                                                                                                        <  4:20289/149‑1 (MQ=255)
        gcaggGCAAAGCCTGCGGTCTTGCCGGTACCGGTCTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTT‑GGCCTGCACCGGGGTCGGGGT‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGAt                                                                                                                                      >  7:302104/1‑148 (MQ=255)
        gcaggGCAAAGCCTGCGGTCTTGCCGGTACCGGTCTGGGCGGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTT‑GGCCTGCACCGGGGTCGGGGT‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATc                                                                                                                                     <  6:257506/149‑1 (MQ=255)
         caggGCAAAGCCTGCGGTCTTGCCGGTACCGGTCTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTT‑GGCCTGCACCGGGGTCGGGGT‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCa                                                                                                                                    <  6:110547/149‑1 (MQ=255)
                    cTGCGGTCTTGCCGGTACCGGTCTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTT‑GGCCTGCACCGGGGTCGGGGT‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTg                                                                                                                         <  1:291417/149‑1 (MQ=255)
                     tGCGGTCTTGCCGGTACCGGTCTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTT‑GGCCTGCACCGGGGTCGGGGT‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTgg                                                                                                                        >  6:332136/1‑149 (MQ=255)
                      gCGGTCTTGCCGGTACCGGTCTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTT‑GGCCTGCACCGGGGTCGGGGT‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGc                                                                                                                       <  7:256510/149‑1 (MQ=255)
                      gCGGTCTTGCCGGTACCGGTCTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTT‑GGCCTGCACCGGGGTCGGGGT‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGc                                                                                                                       <  8:302104/149‑1 (MQ=255)
                          tCTTGCCGGTACCGGTCTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTT‑GGCCTGCACCGGGGTCGGGGT‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGaaa                                                                                                                   <  5:255563/149‑1 (MQ=255)
                               ccGGTACCGGTCTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTT‑GGCCTGCACCGGGGTCGGGGT‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCAt                                                                                                              <  5:139106/149‑1 (MQ=255)
                                    aCCGGTCTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTT‑GGCCTGCACCGGGGTCGGGGT‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCaa                                                                                                         >  2:250757/1‑149 (MQ=255)
                                       ggTCTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTT‑GGCCTGCACCGGGGTCGGGGT‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATAc                                                                                                      >  7:321259/1‑149 (MQ=255)
                                             ggCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTT‑GGCCTGCACCGGGGTCGGGGT‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGt                                                                                                   <  5:229235/146‑1 (MQ=255)
                                             ggCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTT‑GGCCTGCACCGGGGTCGGGGT‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCa                                                                                                 <  1:349249/148‑1 (MQ=255)
                                                tGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTT‑GGCCTGCACCGGGGTCGGGGT‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAgggg                                                                                             <  7:171393/149‑1 (MQ=255)
                                                tGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTT‑GGCCTGCACCGGGGTCGGGGT‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAgggg                                                                                             >  2:104120/1‑149 (MQ=255)
                                                   ggCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTT‑GGCCTGCACCGGGGTCGGGGT‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGtt                                                                                          >  2:201081/1‑149 (MQ=255)
                                                   ggCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTT‑GGCCTGCACCGGGGTCGGGGT‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGtt                                                                                          >  8:329879/1‑149 (MQ=255)
                                                     ccATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTT‑GGCCTGCACCGGGGGCGGGGT‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCa                                                                                        <  7:297033/149‑1 (MQ=255)
                                                          aGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTT‑GGCCTGCACCGGGGTCGGGGT‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCg                                                                                     >  6:341739/1‑147 (MQ=255)
                                                           ggTCGCGGCCGGCCAGCACGGCGGGGATGGCTTT‑GGCCTGCACCGGGGTCGGGGT‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGAAg                                                                                  <  1:341101/149‑1 (MQ=255)
                                                              cgcgGCCGGCCAGCACGGCGGGGATGGCTTT‑GGCCTGCACCGGGGTCGGGGT‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGAAgg‑cg                                                                              >  2:189467/1‑149 (MQ=255)
                                                                  gccggccAGCACGGCGGGGATGGCTTT‑GGCCTGCACCGGGGTCGGGGT‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGAAGG‑CGGCaa                                                                          <  8:101404/149‑1 (MQ=255)
                                                                        cAGCACGGCGGGGAGGGCGTT‑GGCCTGCACCGGGGGCGGGGT‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGACCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGAAGG‑CGGCAAGTTTAc                                                                    <  8:146869/149‑1 (MQ=255)
                                                                         aGCACGGCGGGGATGGCTTT‑GGCCTGCACCGGGGTCGGGGT‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGATCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGAAGG‑CGGCAAGTTTAcc                                                                   <  8:9269/149‑1 (MQ=255)
                                                                               gcggGGATGGCTTT‑GGCCTGCACCGGGGTCGGGGT‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGCCAATACCGTCAGGGGGTTCAGCGAAGC‑CGGCAAGTTTACCGCATCg                                                             >  7:48675/1‑149 (MQ=255)
                                                                                 ggggATGGCTTT‑GGCCTGCACCGGGGTCGGGGTGGGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGAAGG‑CGGCAAGTTTACCGCATCGc                                                            >  1:56933/1‑149 (MQ=255)
                                                                                     aTGGCTTT‑GGCCTGCACCGGGGTCGGGGT‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGAAGG‑CGGCAAGTTTACCGCATCGCCCCTg                                                       >  3:168519/1‑149 (MQ=255)
                                                                                      tGGCTTT‑GGCCTGCACCGGGGTCGGGGT‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGAAGG‑CGGCAAGTTTACCGCATCGCCCCTGc                                                      <  1:169777/149‑1 (MQ=255)
                                                                                       ggCTTT‑GGCCTGCACCGGGGTCGGGGT‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGAAGG‑CGGCAAGTTTACCGCATCGCCCCTGcc                                                     >  8:8754/1‑149 (MQ=255)
                                                                                          ttt‑GGCCTGCACCGGGGTCGGGGT‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGAAGG‑CGGCAAGTTTACCGCATCGCCCCTGCCTAc                                                  >  5:120855/1‑149 (MQ=255)
                                                                                            t‑GGCCTGCACCGGGGTCGGGGT‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGAAGG‑CGGCAAGTTTACCGCATCGCCCCTGCCTAc                                                  <  5:332136/147‑1 (MQ=255)
                                                                                                ccTGCACCGGGGTCGGGGT‑GGTGTAGTCCAGTTTCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTTGCGAAATTAATGGCAATACCCTCAGGGGGTTCAGCGAAGG‑CGGCAAGTTTAACGCATAGCCCCTGCCTACATGc                                              >  3:8474/1‑148 (MQ=255)
                                                                                                 cTGCACCGGGGTCGGGGT‑GGTGTAGTCCAGTTGCTGCGGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGAAGG‑CGGCAAGTTTACCGCATCGCCCCTGCCTACTTg                                               >  3:279914/1‑146 (MQ=255)
                                                                                                    cACCGGGGTCGGGGT‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGAAGG‑CGGCAAGTTTACCGCATCGCCCCTGCCTACTTGCAGAtt                                         >  8:277772/1‑149 (MQ=255)
                                                                                                       cggggtcggggt‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGAAGG‑CGGCAAGTTTACCGCATCGCCCCTGCCTACTTGCAGATTTCa                                      <  2:56933/149‑1 (MQ=255)
                                                                                                        ggggtcggggt‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGAAGG‑CGGCAAGTTTACCGCATCGCCCCTGCCTACTTGCAGATTTCAc                                     <  2:379334/149‑1 (MQ=255)
                                                                                                         gggtcggggt‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGAAGG‑CGGCAAGTTTACCGCATCGCCCCTGCCTACTTGCAg                                            >  8:311392/1‑141 (MQ=255)
                                                                                                         gggtcggggt‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGAAGG‑CGGCAAGTTTACCGCATCGCCCCTGCCTACTTGCAg                                            <  7:311392/141‑1 (MQ=255)
                                                                                                           gtcggggt‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATgg                                                                                                            <  1:115558/76‑1 (MQ=255)
                                                                                                           gtcggggt‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATgg                                                                                                            >  2:115558/1‑76 (MQ=255)
                                                                                                            tcggggt‑GGTGTAGTCCAGTTGCTGCAGCGTGAGCAGCAGCGGTTCGATCAGGACGAGTTTGGCGAAATTCATGGCAATACCGTGAGGGGGTTCAGCGAAGG‑CGGCAAGTTTAGCGCATCGCCCCTGCCTACTTGCAGATTTCACGttt                                 <  8:261307/149‑1 (MQ=255)
                                                                                                              ggggt‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGAAGG‑CGGCAAGTTTACCGCATCGccc                                                          <  8:271654/122‑1 (MQ=255)
                                                                                                              ggggt‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGAAGG‑CGGCAAGTTTACCGCATCGccc                                                          >  7:271654/1‑122 (MQ=255)
                                                                                                              ggggt‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGAAGG‑CGGCAAGTTTACCGCATCGCCCCTGCCTACTTGCAGATTTCACCTTTc                                <  1:165409/148‑1 (MQ=255)
                                                                                                                ggt‑ggtGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGAAGG‑CGGCAAGTTTACCGCATCGCCCCTGCCTACTTGCAGATTTCACCTTTCAAc                             <  7:277772/149‑1 (MQ=255)
                                                                                                                  t‑ggtgTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGAAGG‑CGGCAAGTTTACCGCATCGCCCCTGCCTACTTGCAGATTTCACCTTTCAACgg                           >  5:95629/1‑149 (MQ=255)
                                                                                                                      tgtAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGAAGGACGGCAAGTTTACCGCATCGCCCCTGCCTACTTGCAGATTTCACCTTTCAACGGtt                         <  1:104120/149‑1 (MQ=255)
                                                                                                                       gtAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGAAGG‑CGGCAAGTTTACCGCATCGCCCCTGCCTACTTGCAGATTTCACCTTTCAACGGTTg                        >  7:203593/1‑148 (MQ=255)
                                                                                                                       gtAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGAAGG‑CGGCAAGTTTACCGCATCGCCCCTGCCTACTTGCAGATTTCACCTTTCAACGGTTg                        >  4:45555/1‑148 (MQ=255)
                                                                                                                        tacgccAGGTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGAAGG‑CGGCAAGTTTACCGCATCGCCCCTGCCTACTTGCAGATTTCACCTTTCAACGGTTGTa                      <  8:321259/145‑1 (MQ=255)
                                                                                                                        tAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGAAGG‑CGGCAAGTTTACCGCATCGCCCCTGCCTACTTGCAGATTTCACCTTTCAACGGTTGTa                      <  1:250757/149‑1 (MQ=255)
                                                                                                                         aGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGAAGG‑CGGCAAGTTTACCGCATCGCCCCTGCCTACTTGCAGATTTCACCTTTCAACGGTTGTa                      >  6:149404/1‑148 (MQ=255)
                                                                                                                          gTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGAAGG‑CGGCAAGTTTACCGCATCGCCCCTGCCTACTTGCAGATTTCACCTTTCAACGGTTGTAc                     <  5:341739/148‑1 (MQ=255)
                                                                                                                          gTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGAAGG‑CGGCAAGTTTACCGCATCGCCCCTGCCTACTTGCAGATTTCACCTTTCAACGGTTGTACa                    <  6:95629/149‑1 (MQ=255)
                                                                                                                             cAGTTGCTGCAGCGTGCGCAGCAGCGGTACGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGAAGG‑CGGCAAGTTTACCGCATCGCCCCTGCCTACTTGCAGATTTCACCTTTCAACGGTTGTACAGGt                 >  2:234124/1‑149 (MQ=255)
                                                                                                                              aGTTGCTGCAGCGTTCGCATCAGCGGTTGGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGAAGG‑CGGCAAGTTTACCGCATCGCGCCTGCCTACTTGCAGATTTCACCTTTCAACGGTTGTACAGGtt                <  8:48675/149‑1 (MQ=255)
                                                                                                                               gTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGAAGG‑CGGCAAGTTTACCGCATCGCCCCTGCCTACTTGCAGATTTCACCTTTCAACGGTTGTACAGGtt                >  8:305850/1‑148 (MQ=255)
                                                                                                                               accgctgctGCGCACGCTGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGaa                                                                                   >  3:298697/4‑82 (MQ=38)
                                                                                                                               accgctgctGCGCACGCTGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGaa                                                                                   <  4:298697/79‑1 (MQ=38)
                                                                                                                                ccgctgctGCGCACGCTGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGAAGG‑CGGCAAGTTTACCGCATCGCCCCTGCCTACTTGCAGATTTCACCTTTCAACGGTTGTACAGGtt                >  8:241192/3‑147 (MQ=255)
                                                                                                                                 cgctgctGCGCACGCTGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGAAGG‑CGGCAAGTTTACCGCATCGCCCCTGCCTACTTGCAGATTTCACCTTTCAACGGTTGTACAGGTTCAg             >  1:360624/2‑149 (MQ=255)
                                                                                                                                     gcAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGAAGG‑CGGCAAGTTTACCGCATCGCCCCTGCCTACTTGCAGATTTCACCTTTCAACGGTTGTACAGGTTCAGgcgc         >  2:87563/1‑149 (MQ=255)
                                                                                                                                          gTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGAAGG‑CGGCAAGTTTACCGCATCGCCCCTGCCTACTTGCAGATTTCACCTTTCAACGGTTGTACAg                   >  1:202741/1‑134 (MQ=255)
                                                                                                                                          gTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGAAGG‑CGGCAAGTTTACCGCATCGCCCCTGCCTACTTGCAGATTTCACCTTTCAACGGTTGTACAg                   <  2:202741/134‑1 (MQ=255)
                                                                                                                                            gcgcAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGAAGG‑CGGCAAGTTTACCGCATCGCCCCTGCCTACTTGCAGATTTCACCTTTCAACGGTTGTACAGGTTCAGGCGCCTTgcgc  >  8:211264/1‑149 (MQ=255)
                                                                                                                                            acgcTGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGAAGG‑CGGCAAGTTTACCGCATCGCCCCTGCCTACTTGCAGATTTCACCTTTCAACGGTTGTACAGGTTCAGGCGCCTTgcgc  <  2:360624/148‑1 (MQ=255)
                                                                                                                                             cgcAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGAAGG‑CGGCAAGTTTACCGCATCGCCCCTGCCTACTTGCAGATTTCACCTTTCAACGGTTGTACAGGTTCAGGCGCCTTgcgc  >  5:90718/1‑148 (MQ=255)
                                                                                                                                             cgcAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGAAGG‑CGGCAAGTTTACCGCATCGCCCCTGCCTACTTGCAGATTTCACCTTTCAACGGTTGTACAGGTTCAGGCGCCTTgcgc  >  2:52413/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
CAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGTCTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTT‑GGCCTGCACCGGGGTCGGGGT‑GGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGCGGTTCGATCAGGCCGAGTTTGGCGAAATTCATGGCAATACCGTCAGGGGGTTCAGCGAAGG‑CGGCAAGTTTACCGCATCGCCCCTGCCTACTTGCAGATTTCACCTTTCAACGGTTGTACAGGTTCAGGCGCCTTGCGC  >  NC_002947/5427923‑5428209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑