Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,430,410 C→T 6.6% intergenic (‑92/+69) PP_4769 ← / ← PP_4770 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,430,4100CT 6.6% 172.7 / 2.4 60intergenic (‑92/+69)PP_4769/PP_4770hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (26/30);  new base T (2/2);  total (28/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.50e-01

CTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCGGCGTATCCGCCCTGGTCAC  >  NC_002947/5430262‑5430558
                                                                                                                                                       |                                                                                                                                                    
cTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACAccc                                                                                                                                                      <  3:261127/149‑1 (MQ=255)
  ggCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATGTTTTTCACCTGGGAACCGAATTTTTGGCCTGGAGTGCCG‑‑GGGGGCTGCTGAGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGt                                                                                                                                                    >  5:157118/1‑149 (MQ=255)
   gcgcACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTg                                                                                                                                                   <  5:117947/149‑1 (MQ=255)
      cACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGCGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTgg                                                                                                                                                  <  1:112925/149‑1 (MQ=255)
        cGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACGGGTGTACGCGCG‑ATACCTGTGGGAGc                                                                                                                                              <  5:337135/149‑1 (MQ=37)
         ggTGTAGTAGAGGTTCTGCACATCGGCGTACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCg                                                                                                                                             >  1:211774/1‑149 (MQ=255)
          gtgtAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCgg                                                                                                                                            <  2:210042/149‑1 (MQ=255)
             tagtagACGGTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGc                                                                                                                                          <  7:219095/148‑1 (MQ=255)
                tagAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGc                                                                                                                                      <  5:143817/149‑1 (MQ=255)
                tagAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑‑GGGGGCTGCTGCGCAGACCTTCGCGG‑CACGCCCGCTCCCACGGGTGTACGCGCG‑ATACCTGTGGGAGCGGGCGTgtc                                                                                                                                     >  5:207109/1‑147 (MQ=25)
                  gagGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGc                                                                                                                                          <  5:358503/143‑1 (MQ=255)
                  gagGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGc                                                                                                                                          >  6:358503/1‑143 (MQ=255)
                  gagGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑‑GGGGGCTGCTGCGCAGACCTTCGCGG‑CACGCCCGCTCCCACGGGTGTACGCGCG‑ATACCTGTGGGAGCGGGCGTGTCcg                                                                                                                                   <  6:207109/149‑1 (MQ=25)
                   agGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCcg                                                                                                                                   >  6:358358/1‑149 (MQ=255)
                      ttCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCcgcg                                                                                                                                 >  8:235999/1‑148 (MQ=255)
                         agCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGTGAAg                                                                                                                              <  5:166989/147‑1 (MQ=255)
                          gCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAggg                                                                                                                            >  2:89595/1‑149 (MQ=255)
                              aTCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGgcg                                                                                                                         >  4:40103/1‑148 (MQ=255)
                              aTCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGgcg                                                                                                                         >  4:312777/1‑148 (MQ=255)
                                 ggCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCaaa                                                                                                                     <  2:211774/149‑1 (MQ=255)
                                 ggCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCaaa                                                                                                                     >  4:133235/1‑149 (MQ=255)
                                   cGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCCCCCCCCCACGTTT‑AGCGCGGGAACCCCGGGGGGCGGGCGGGGCCCGGCAGGGGCGCcaacg                                                                                                                   >  2:354319/1‑147 (MQ=255)
                                    gAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAgcg                                                                                                                  >  8:114088/1‑149 (MQ=255)
                                      aCAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGcc                                                                                                                <  6:165996/149‑1 (MQ=255)
                                      aCAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGcc                                                                                                                <  6:107874/149‑1 (MQ=255)
                                        aGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAgcgc                                                                                                                 >  7:342642/1‑146 (MQ=255)
                                        aGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAgcgc                                                                                                                 <  8:342642/146‑1 (MQ=255)
                                           gcgATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCg                                                                                                            >  3:17256/1‑148 (MQ=255)
                                                   tttCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGaaa                                                                                                        <  3:175223/144‑1 (MQ=255)
                                                   tttCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑‑GGAGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAAt                                                                                                       <  3:368777/145‑1 (MQ=255)
                                                    ttCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGTCCCAAAGCGCCCCCGGAAATGCca                                                                                                   <  7:235999/148‑1 (MQ=255)
                                                       aCCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCcagcag                                                                                               >  5:66081/1‑149 (MQ=255)
                                                                 cTCATTTTTGGCCTGGAGTGCCG‑‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGAc                                                                                     >  7:95660/1‑149 (MQ=255)
                                                                     tttttGGCCTGGAGTGCCG‑‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCAACAGGTAT‑CGCGCGTACACCCGTGGTAGGGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGt                                                                                 <  1:89595/149‑1 (MQ=255)
                                                                           gCCTGGAGTGCCG‑‑GGGGGCTGCTGCGCAGACCTGCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGAc                                                                            <  7:128191/148‑1 (MQ=255)
                                                                                      cG‑‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAAtcgtc                                                                <  8:6523/149‑1 (MQ=255)
                                                                                          gggggCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATcgtcgt                                                              <  3:333180/149‑1 (MQ=255)
                                                                                            gggCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTcc                                                            <  5:305184/149‑1 (MQ=255)
                                                                                            gggCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTcc                                                            <  4:17256/149‑1 (MQ=255)
                                                                                              gctgctGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTcc                                                            >  8:331102/1‑147 (MQ=255)
                                                                                              gctgctGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCga                                                          <  3:133235/149‑1 (MQ=255)
                                                                                                  ctgcGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGagaa                                                       <  6:66081/148‑1 (MQ=255)
                                                                                                     cgcAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGGCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAAc                                                   >  5:293308/1‑149 (MQ=255)
                                                                                                          aCCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATcgtcgt                                                              <  7:254545/133‑1 (MQ=255)
                                                                                                          aCCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATcgtcgt                                                              >  8:254545/1‑133 (MQ=255)
                                                                                                               cgcgGACACGCCGGCTCCCACAGGTAT‑CGCGCGTACCCCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTc                                           <  1:371641/147‑1 (MQ=255)
                                                                                                                gcgGACACGCCCCCTCCCACAGGTAT‑CGCGCGTAAACCCGTGGGAGCGGGCCTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTAAGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATc                                        >  6:19161/1‑149 (MQ=255)
                                                                                                                 cgGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCg                                       >  1:30132/1‑149 (MQ=255)
                                                                                                                     cacGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGc                                   <  3:40103/149‑1 (MQ=255)
                                                                                                                      acGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGcc                                  <  4:27791/149‑1 (MQ=255)
                                                                                                                      acGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGc                                   >  6:199382/1‑148 (MQ=255)
                                                                                                                      acGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGc                                   >  2:45839/1‑148 (MQ=255)
                                                                                                                        gCCCGCTCCCACGGGTGTACGCGCG‑ATACCTGTGGGAGCGGGCGTGCGCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCa                                 >  2:34534/1‑148 (MQ=37)
                                                                                                                        gCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGCACCCACCGTCATCGCTGCCa                                 >  1:53600/1‑148 (MQ=255)
                                                                                                                        gCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCaa                                <  6:233812/149‑1 (MQ=255)
                                                                                                                              tCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGACAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCAACGCTGCCCAAaccac                          >  8:165304/1‑149 (MQ=255)
                                                                                                                                     ggTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCGGc                   <  7:114088/149‑1 (MQ=255)
                                                                                                                                             cgcgTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGAAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCGGCGTATCCg            >  7:242768/1‑149 (MQ=255)
                                                                                                                                                gTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTc                      >  5:314368/1‑136 (MQ=255)
                                                                                                                                                gTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTc                      <  6:314368/136‑1 (MQ=255)
                                                                                                                                                    acCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCGGCGTATCCGCCCTGGt     <  5:358358/149‑1 (MQ=255)
                                                                                                                                                      ccGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCGGCGTATCCGCCCTGGTc    >  3:330309/1‑148 (MQ=255)
                                                                                                                                                      ccGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCGGCGTATCCGCCCTGGTCa   >  3:186603/1‑149 (MQ=255)
                                                                                                                                                      ccGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCGGCGTATCCGCCCTGGTCa   >  3:243462/1‑149 (MQ=255)
                                                                                                                                                      ccGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCGGCGTAT‑CGCCCTggggcc  >  7:133461/1‑145 (MQ=255)
                                                                                                                                                       cGTGGGAGCGGGCGTGCCCGCGACGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCGGCGTATCCGCCCTGGTCAc  >  5:255954/1‑149 (MQ=255)
                                                                                                                                                       cGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCGGCGTATCCGCCCTGGTCAc  <  6:112941/149‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                    
CTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCGGCGTATCCGCCCTGGTCAC  >  NC_002947/5430262‑5430558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑