Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 478,984 T→C 6.7% Q162R (CAG→CGG) ‡ cca ← multifunctional tRNA nucleotidyltransferase/2',3'‑cyclic phosphodiesterase/2' nucleotidase/phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947478,9840TC 6.7% 308.8 / 8.7 104Q162R (CAG→CGG) ‡ccamultifunctional tRNA nucleotidyltransferase/2',3'‑cyclic phosphodiesterase/2' nucleotidase/phosphatase
Reads supporting (aligned to +/- strand):  ref base T (47/50);  new base C (3/4);  total (50/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01

Only 100 of 104 total aligned reads displayed.

GAGCTCAGGCAGCAGCTCCTGCAGGGCCCCACACGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACATGGCGTAGCAGGCGTTGATCCAGATCGTTC  >  NC_002947/478839‑479130
                                                                                                                                                 |                                                                                                                                                    
gAGCTCAGGCAGCAGCTCCTGCAGGGCCCCACACGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTgg                                                                                                                                                    <  1:124566/148‑1 (MQ=255)
  gCTCAGGCAGCAGCTCCTGCAGGGCCCCACACGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGgcgc                                                                                                                                                 <  5:254526/149‑1 (MQ=255)
  gCTCAGGCAGCAGCTCCTGCAGGGCCCCACACGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGgcg                                                                                                                                                  >  2:266735/1‑148 (MQ=255)
    tCAGGCAGCAGCTCCTGCAGGGCCCCACACGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGgc                                                                                                                                                   <  2:37269/145‑1 (MQ=255)
    tCAGGCAGCAGCTCCTGCAGGGCCCCACACGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGgc                                                                                                                                                   >  1:37269/1‑145 (MQ=255)
     cAGGCAGCAGCTCCTGCAGGGCCCCACACGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATc                                                                                                                                              >  7:172651/1‑149 (MQ=255)
        gcagcaGCTCCTGCAGGGCCCCACACGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAgc                                                                                                                                           >  2:221542/1‑149 (MQ=255)
         cagcagCTCCTGCAGGGCCCCACACGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAgcg                                                                                                                                          <  8:281167/149‑1 (MQ=255)
          agcagcTCCTGCAGGGCCCCACACGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATGCGCCAGATCAgcgc                                                                                                                                         <  6:144909/149‑1 (MQ=255)
          agcagcTCCTGCAGGGCCCCACACGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATGCGCCAGATCAgcgc                                                                                                                                         <  8:133857/149‑1 (MQ=255)
          agcagcTCCTGCAGGGCCCCACACGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTCCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAgcgc                                                                                                                                         >  4:289845/1‑149 (MQ=255)
               cTCCTGCAGGGCCCCACACGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAgg                                                                                                                                    >  6:223021/1‑149 (MQ=255)
                 ccTGCAGGGCCCCACACGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGtt                                                                                                                                  >  7:111461/1‑149 (MQ=255)
                  cTGCAGGGCCCCACACGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGttt                                                                                                                                 <  4:77670/149‑1 (MQ=255)
                  cTGCAGGGCCCCACACGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGttt                                                                                                                                 <  5:21423/149‑1 (MQ=255)
                     cAGGGCCCCACACGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCAt                                                                                                                              <  5:99618/149‑1 (MQ=255)
                        ggCCCCACACGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCaa                                                                                                                                      <  7:263217/138‑1 (MQ=255)
                        ggCCCCACACGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCaa                                                                                                                                      >  8:263217/1‑138 (MQ=255)
                        ggCCCCACACGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCgg                                                                                                                           >  4:258923/1‑149 (MQ=255)
                        ggCCCCACACGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCgg                                                                                                                           <  3:285305/149‑1 (MQ=255)
                        ggCCCCACACGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCgg                                                                                                                           >  7:102055/1‑149 (MQ=255)
                              acacGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAAccc                                                                                                                     >  6:154400/1‑149 (MQ=255)
                              acacGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAAccc                                                                                                                     >  3:161220/1‑149 (MQ=255)
                                  gcgcTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGaa                                                                                                                 <  2:183049/149‑1 (MQ=255)
                                  gcgcTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGaa                                                                                                                 <  1:354290/149‑1 (MQ=255)
                                    gcTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAAcc                                                                                                               >  2:28566/1‑149 (MQ=255)
                                        aGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAg                                                                                                           <  6:304916/149‑1 (MQ=255)
                                         gCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAgt                                                                                                          >  6:127689/1‑149 (MQ=255)
                                         gCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCACCTGCGCTCGGCAGTGAGGTCCTGCAGTTCGCCTGACGCGCTGATCTGGCGCATCAGCGCCAATGTTTCATCGGCAACCCGGAACCCCAgt                                                                                                          <  6:179445/149‑1 (MQ=255)
                                            ccTTGAAGAATAGCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAgtggt                                                                                                       <  3:289845/149‑1 (MQ=255)
                                              ttGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGc                                                                                                     >  4:168414/1‑149 (MQ=255)
                                               tGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCg                                                                                                    >  3:100701/1‑149 (MQ=255)
                                                gaagaaTACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGc                                                                                                     <  5:154400/147‑1 (MQ=255)
                                                 aagaaTACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCACCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGt                                                                                                   >  3:177664/1‑148 (MQ=255)
                                                   gaaTACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAAc                                                                                                >  5:90837/1‑149 (MQ=255)
                                                   gaaTACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAAc                                                                                                >  1:73369/1‑149 (MQ=255)
                                                         cTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGgcag                                                                                          >  2:249955/1‑149 (MQ=255)
                                                         cTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGgcag                                                                                          >  6:107729/1‑149 (MQ=255)
                                                            cGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGgcagc                                                                                         <  2:146187/147‑1 (MQ=255)
                                                             ggTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCaaa                                                                                      <  7:189772/149‑1 (MQ=255)
                                                             ggTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCaaa                                                                                      <  3:168414/149‑1 (MQ=255)
                                                               ttGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGc                                                                                    <  3:328016/149‑1 (MQ=255)
                                                                 gCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCg                                                                                   <  8:102055/148‑1 (MQ=255)
                                                                  cACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑t                                                                                 <  4:207234/148‑1 (MQ=255)
                                                                  cACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTCCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑t                                                                                 <  5:223021/148‑1 (MQ=255)
                                                                   aCTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAgtggt                                                                                                       <  6:9479/126‑1 (MQ=255)
                                                                   aCTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑Tg                                                                                >  7:38739/1‑148 (MQ=255)
                                                                     ttCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCaa                                                                             >  6:110840/1‑149 (MQ=255)
                                                                     ttCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCaa                                                                             >  5:170725/1‑149 (MQ=255)
                                                                         aTCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAAcgcg                                                                         <  2:24056/149‑1 (MQ=255)
                                                                         aTCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAAcgcg                                                                         >  4:117160/1‑149 (MQ=255)
                                                                          tCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAAcgcg                                                                         >  1:91807/1‑148 (MQ=255)
                                                                            aGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCgcag                                                                      <  7:6377/149‑1 (MQ=255)
                                                                            aGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCgca                                                                       <  8:111461/148‑1 (MQ=255)
                                                                            aGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCACCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCgca                                                                       <  4:177664/148‑1 (MQ=255)
                                                                                cgcgcTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCAcc                                                                  <  5:127689/149‑1 (MQ=255)
                                                                                    cTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGc                                                               >  4:243131/1‑148 (MQ=255)
                                                                                     tCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCa                                                              >  3:270848/1‑148 (MQ=255)
                                                                                       gATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCaaa                                                            <  1:249955/148‑1 (MQ=255)
                                                                                       gATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCaaa                                                            <  5:107729/148‑1 (MQ=255)
                                                                                        aTTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAgg                                                          <  4:161220/149‑1 (MQ=255)
                                                                                         atttcatccAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑a                                                        >  5:215735/7‑149 (MQ=255)
                                                                                          ttccttccAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑At                                                       >  3:102514/1‑149 (MQ=255)
                                                                                             cttccAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCt                                                     <  8:172651/148‑1 (MQ=255)
                                                                                             cttccAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCAGCAAAGG‑ATAtt                                                    >  4:97681/1‑149 (MQ=255)
                                                                                              ttccAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTc                                                   >  3:334684/1‑149 (MQ=255)
                                                                                              ttccAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTc                                                   >  7:103667/1‑149 (MQ=255)
                                                                                                ccAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCGCTGCAACGCGCAGCACCCGCAAAGG‑ATCtt                                                    <  1:28566/147‑1 (MQ=255)
                                                                                                 cAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGc                                                                                                     >  7:199815/1‑98 (MQ=255)
                                                                                                 cAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGc                                                                                                     <  8:199815/98‑1 (MQ=255)
                                                                                                 cAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCCGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGc                                                <  4:26165/149‑1 (MQ=255)
                                                                                                     tGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAAt                                            >  3:119484/1‑149 (MQ=255)
                                                                                                         cTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGCCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCg                                        <  1:317012/149‑1 (MQ=255)
                                                                                                          tCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCgg                                       >  6:256052/1‑149 (MQ=255)
                                                                                                             gCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCgggg                                     >  3:38341/1‑148 (MQ=255)
                                                                                                                gtgagtgCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAAc                                 <  4:347236/149‑1 (MQ=255)
                                                                                                                 tgagtgCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACa                                >  1:63802/1‑149 (MQ=255)
                                                                                                                   agtgCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACATg                              <  2:73369/149‑1 (MQ=255)
                                                                                                                    gtgCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACATgg                             <  3:117160/149‑1 (MQ=255)
                                                                                                                    gtgCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACATgg                             >  4:159887/1‑149 (MQ=255)
                                                                                                                       ccTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑t                                                                                 <  7:5287/95‑1 (MQ=255)
                                                                                                                       ccTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑t                                                                                 >  8:5287/1‑95 (MQ=255)
                                                                                                                       ccTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGGAATCTTCGGCGAATGCCGGGGAAACATGGc                            <  1:221542/148‑1 (MQ=255)
                                                                                                                           cAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACATGGCGTAg                        <  6:90837/147‑1 (MQ=255)
                                                                                                                                   cTGAGGCTCTTATCTGGCTCATCAGCGCCAAGGTTTCATCGGCAAGCCGGAACCCCAGTGGTGCGTATCGGGCAGCAAAGAG‑TGCAACGCGCAGCACCCGCAAAGG‑ATGTTCTGCGAATTCCGGGGAAAAATGGCGTAGCATGCGTTGa              <  6:215735/149‑1 (MQ=255)
                                                                                                                                   cTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACATGGCGTAGCAGGCGTTGa              >  1:355294/1‑149 (MQ=255)
                                                                                                                                   cTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACATGGCGTAGCAGGCGTTGa              <  2:63802/149‑1 (MQ=255)
                                                                                                                                     ttggCGCTGATGCGCCAGATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACATGGCGTAGCAGGCGt                 >  8:5733/3‑144 (MQ=255)
                                                                                                                                     ttggCGCTGATGCGCCAGATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACATGGCGTAGCAGGCGt                 <  7:5733/142‑1 (MQ=255)
                                                                                                                                     ttggCGCTGATGCGCCAGATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACATGGCGTAGCAGGCGTTGATc            >  8:115332/3‑149 (MQ=255)
                                                                                                                                     ttggCGCTGATGCGCCAGATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGAGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACATGGCGTAGCAGGCGTTGATc            >  5:227498/3‑149 (MQ=255)
                                                                                                                                      aGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACATGGCGTAGCAGGCGTTGATcc           <  4:119484/149‑1 (MQ=255)
                                                                                                                                      aGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACATGGCGTAGCAGGCGTTGATcc           <  3:258923/149‑1 (MQ=255)
                                                                                                                                      aGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACATGGCGTAGCAGGCGTTGATcc           <  7:59854/149‑1 (MQ=255)
                                                                                                                                       ggCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACATGGCGTAGCAGGCGTTGATc            >  8:54401/1‑147 (MQ=255)
                                                                                                                                         cgcTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACATGGCGTAGCAGGCGTTGATCCAGa        <  4:100701/149‑1 (MQ=255)
                                                                                                                                          gcTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACATGGCGTAGCAGGCGTTGATc            >  6:374261/1‑144 (MQ=255)
                                                                                                                                              aTCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACATGGCGTAGCAGGCGTTGATCCAGATCGtt   >  4:325473/1‑149 (MQ=255)
                                                                                                                                              aTCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACATGGCGTAGCAGGCGTTGATCCAGATCGtt   >  4:330845/1‑149 (MQ=255)
                                                                                                                                                cTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGTAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACATGGCGTAGCAGGCGTTGATCCAGATCGTTc  <  3:243131/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GAGCTCAGGCAGCAGCTCCTGCAGGGCCCCACACGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACATGGCGTAGCAGGCGTTGATCCAGATCGTTC  >  NC_002947/478839‑479130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑