Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,518,544 G→C 5.1% P13A (CCC→GCC)  PP_4850 ← membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,518,5440GC 5.1% 256.2 / 3.4 78P13A (CCC→GCC) PP_4850membrane protein
Reads supporting (aligned to +/- strand):  ref base G (34/40);  new base C (2/2);  total (36/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.37e-01

CCAGTTCGAACACCAGCAGACCGGCCAGCAGGCTGGGCAGCAACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTTGGTGGTGGCGTCAGCATTGCAGGTCTTCATTTTTT  >  NC_002947/5518397‑5518684
                                                                                                                                                   |                                                                                                                                            
ccAGTTCGAACACCAGCAGACCGGCCAGCAGGCTGGGCAGCAACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCggg                                                                                                                                              >  2:25499/1‑148 (MQ=255)
   gTTCGAACACCAGCAGACCGGCCAGCAGGCTGGGCAGCAACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATg                                                                                                                                          >  5:106699/1‑149 (MQ=255)
    ttCGAACACCAGCAGACCGGCCAGCAGGCTGGGCAGCAACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGc                                                                                                                                         >  4:51849/1‑149 (MQ=255)
    ttCGAACACCAGCAGACCGGCCAGCAGGCTGGGCAGCAACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGc                                                                                                                                         <  6:249559/149‑1 (MQ=255)
       gAACACCAGCAGACCGGCCAGCAGGCTGGGCAGCAACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCg                                                                                                                                       >  4:319129/1‑148 (MQ=255)
        aaCACCAGCAGACCGGCCAGCAGGCTGGGCAGCAACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCa                                                                                                                                     <  4:290352/149‑1 (MQ=255)
        aaCACCAGCAGACCGGCCAGCAGGCTGGGCAGCAACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCa                                                                                                                                     <  3:298501/149‑1 (MQ=255)
               gcagACCGGCCAGCAGGCTGGGCAGCAACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGc                                                                                                                               >  5:309741/1‑148 (MQ=255)
                cagACCGGCCAGCAGGCTGGGCAGCAACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCa                                                                                                                             <  1:136918/149‑1 (MQ=255)
                        ccAGCAGGCTGGGCAGCAACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTggc                                                                                                                     <  4:332584/149‑1 (MQ=255)
                        ccAGCAGGCTGGGCAGCAACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTggc                                                                                                                     >  3:332584/1‑149 (MQ=255)
                               gCTGGGCAGCAACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTggc                                                                                                                     <  3:78131/142‑1 (MQ=255)
                               gCTGGGCAGCAACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTggc                                                                                                                     >  4:78131/1‑142 (MQ=255)
                               gCTGGGCAGCAACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAg                                                                                                              >  1:126670/1‑149 (MQ=255)
                                 tGGGCAGCAACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAgg                                                                                                             >  7:71259/1‑148 (MQ=255)
                                   ggCAGCAACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCAt                                                                                                          <  1:25499/149‑1 (MQ=255)
                                    gcagcaACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCa                                                                                                           >  6:324031/1‑147 (MQ=255)
                                     cagcaACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAg                                                                                                        <  8:60998/149‑1 (MQ=255)
                                     cagcaACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAg                                                                                                        <  5:324031/149‑1 (MQ=255)
                                       gcaACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACTGCGGCATTCCCGCTGTCTGccg                                                                                                                             >  8:272656/1‑125 (MQ=255)
                                       gcaACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACTGCGGCATTCCCGCTGTCTGccg                                                                                                                             <  7:272656/126‑2 (MQ=255)
                                       gcaACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGgc                                                                                                      >  6:114390/1‑149 (MQ=255)
                                          aCTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCa                                                                                                   <  8:77206/149‑1 (MQ=255)
                                           cTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTTATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAg                                                                                                  <  3:91343/149‑1 (MQ=255)
                                              cAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTggcgg                                                                                                                   >  7:81069/1‑129 (MQ=255)
                                              cAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTggcgg                                                                                                                   <  8:81069/129‑1 (MQ=255)
                                              cAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAgg                                                                                                 <  7:25319/147‑1 (MQ=255)
                                                ggggCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCAt                                                                                             <  5:114390/149‑1 (MQ=255)
                                                   gCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATttc                                                                                          <  6:358712/149‑1 (MQ=255)
                                                   gCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATttc                                                                                          >  5:358712/1‑149 (MQ=255)
                                                   gCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATttc                                                                                          >  7:172631/1‑149 (MQ=255)
                                                     aaaGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATttctt                                                                                        >  8:250423/1‑149 (MQ=255)
                                                         ccAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCTTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATttcttcttc                                                                                    <  5:337591/149‑1 (MQ=255)
                                                           agcagcagcCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAg                                                                                  >  5:233581/1‑149 (MQ=255)
                                                           agcagcagcCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAg                                                                                  <  5:268963/149‑1 (MQ=255)
                                                              agcagcCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGc                                                                                >  2:180698/1‑148 (MQ=255)
                                                                  gcCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCg                                                                           <  3:51849/149‑1 (MQ=255)
                                                                   cccGGCCATGACGATGCTCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGGCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGGCATTTCTTCTTCAGGCAATCGc                                                                          <  6:379961/149‑1 (MQ=255)
                                                                     cGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCt                                                                         >  2:177082/1‑148 (MQ=255)
                                                                     cGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTc                                                                        <  8:71259/149‑1 (MQ=255)
                                                                          aTGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATg                                                                     >  5:338643/1‑147 (MQ=255)
                                                                                 tgatCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTg                                                            <  6:309741/149‑1 (MQ=255)
                                                                                  gatCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTgc                                                           >  5:253014/1‑149 (MQ=255)
                                                                                   atCAGGGTGGCCAGGGTGTTCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGTCAATCGCTCATGAAGGCCTTgcg                                                          <  4:280663/149‑1 (MQ=255)
                                                                                        gCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCAt                                                     >  7:205003/1‑149 (MQ=255)
                                                                                           ggCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATccc                                                  >  3:292402/1‑149 (MQ=255)
                                                                                            gCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATcc                                                   <  8:205003/147‑1 (MQ=255)
                                                                                            gCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATcc                                                   <  1:177082/147‑1 (MQ=255)
                                                                                              cAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCt                                               >  3:211949/1‑149 (MQ=255)
                                                                                                gggTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTc                                             <  4:236589/149‑1 (MQ=255)
                                                                                                  gTGATCTGACGGTGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGTGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCa                                            <  7:68661/148‑1 (MQ=255)
                                                                                                   tGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAgg                                          >  3:194641/1‑149 (MQ=255)
                                                                                                    gATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTgg                                                                                                                      <  5:70084/72‑1 (MQ=255)
                                                                                                    gATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTgg                                                                                                                      >  6:70084/1‑72 (MQ=255)
                                                                                                        tGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAg                                                                                                        <  8:21309/82‑1 (MQ=255)
                                                                                                        tGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAg                                                                                                        >  7:21309/1‑82 (MQ=255)
                                                                                                          aCGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTtgg                                   <  3:319129/149‑1 (MQ=255)
                                                                                                             gggggTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTc                                                                        <  1:207573/109‑1 (MQ=255)
                                                                                                             gggggTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTc                                                                        >  2:207573/1‑109 (MQ=255)
                                                                                                                    aaGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTTGGTGGTGGCGt                          <  2:126670/148‑1 (MQ=255)
                                                                                                                      ggTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTTGGTGGTGGCGTCAg                       <  7:250423/149‑1 (MQ=255)
                                                                                                                       gTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTATTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTTGGTTGTGGCGTCAg                       >  5:85674/1‑148 (MQ=255)
                                                                                                                        tCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTTGGTGGTGGCGTCAGCa                     <  2:141784/149‑1 (MQ=255)
                                                                                                                          aTACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTtggtggt                               >  3:229739/1‑137 (MQ=255)
                                                                                                                          aTACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTtggtggt                               <  4:229739/137‑1 (MQ=255)
                                                                                                                          aTACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTTGGTGGTGGCGTCAGCAtt                   >  2:275458/1‑149 (MQ=255)
                                                                                                                             cAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTTGGTGGTGGCGTCAGCATTGc                 <  6:106699/148‑1 (MQ=255)
                                                                                                                                ccTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTTGGTGGTGGCGTCAGCCTTGCAGGt             >  7:290209/1‑149 (MQ=255)
                                                                                                                                 cTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTTGGTGGTGGCGTCAGCATTGCAGGTc            >  2:32880/1‑149 (MQ=255)
                                                                                                                                 cTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGCGCTTTGGTGGTGGCGTCAGCATTGCAGGTc            <  1:32880/149‑1 (MQ=255)
                                                                                                                                  tCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTTGGTGGTGGCGTCAGCATTGCAGGTCt           >  5:208178/1‑149 (MQ=255)
                                                                                                                                    gctggCAGACTGCGGCATTCCCGCTGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCtt                                              <  6:11541/109‑1 (MQ=38)
                                                                                                                                    gctggCAGACTGCGGCATTCCCGCTGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCtt                                              >  5:11541/4‑112 (MQ=38)
                                                                                                                                    gACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTTGGTGGTGGCGTCAGCATTGCAGGTCTTc         <  6:338643/149‑1 (MQ=255)
                                                                                                                                    gACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGCCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTTGGTGGTGGc                            >  5:24476/1‑130 (MQ=255)
                                                                                                                                    gACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGCCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTTGGTGGTGGc                            <  6:24476/130‑1 (MQ=255)
                                                                                                                                           gACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTTGGTGGTGGCGTCAGCATTGCAGGTCTTCAtttttt  <  4:292402/149‑1 (MQ=255)
                                                                                                                                              aGCGGGAATGCCGCAGTCTGCGAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCTGGGCTTTGGTGGTGGCGTCAGCATTGCAGGTCTTCAttt     <  5:271635/143‑1 (MQ=255)
                                                                                                                                              aGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCCTCCCCCTTCAGGCCTTTGGTGGTGGCGTCAGCATTGCAGGTCTTCAttt     >  6:271635/1‑143 (MQ=255)
                                                                                                                                                   |                                                                                                                                            
CCAGTTCGAACACCAGCAGACCGGCCAGCAGGCTGGGCAGCAACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTTGGTGGTGGCGTCAGCATTGCAGGTCTTCATTTTTT  >  NC_002947/5518397‑5518684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑