Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,518,548	G→C	5.2%	G11G (GGC→GGG)	PP_4850 ←	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,518,548	0	G	C	5.2%	251.6 / 3.3	77	G11G (GGC→GGG)	PP_4850	membrane protein
Reads supporting (aligned to +/- strand):  ref base G (33/40);  new base C (2/2);  total (35/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01

GTTCGAACACCAGCAGACCGGCCAGCAGGCTGGGCAGCAACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTTGGTGGTGGCGTCAGCATTGCAGGTCTTCATTTTTT  >  NC_002947/5518400‑5518684                                                                                                                                                     |                                                                                                                                         gTTCGAACACCAGCAGACCGGCCAGCAGGCTGGGCAGCAACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATg                                                                                                                                          >  5:106699/1‑149 (MQ=255)  ttCGAACACCAGCAGACCGGCCAGCAGGCTGGGCAGCAACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGc                                                                                                                                         >  4:51849/1‑149 (MQ=255)  ttCGAACACCAGCAGACCGGCCAGCAGGCTGGGCAGCAACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGc                                                                                                                                         <  6:249559/149‑1 (MQ=255)     gAACACCAGCAGACCGGCCAGCAGGCTGGGCAGCAACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCg                                                                                                                                       >  4:319129/1‑148 (MQ=255)      aaCACCAGCAGACCGGCCAGCAGGCTGGGCAGCAACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCa                                                                                                                                     <  4:290352/149‑1 (MQ=255)      aaCACCAGCAGACCGGCCAGCAGGCTGGGCAGCAACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCa                                                                                                                                     <  3:298501/149‑1 (MQ=255)             gcagACCGGCCAGCAGGCTGGGCAGCAACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGc                                                                                                                               >  5:309741/1‑148 (MQ=255)              cagACCGGCCAGCAGGCTGGGCAGCAACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCa                                                                                                                             <  1:136918/149‑1 (MQ=255)                      ccAGCAGGCTGGGCAGCAACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTggc                                                                                                                     <  4:332584/149‑1 (MQ=255)                      ccAGCAGGCTGGGCAGCAACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTggc                                                                                                                     >  3:332584/1‑149 (MQ=255)                             gCTGGGCAGCAACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTggc                                                                                                                     <  3:78131/142‑1 (MQ=255)                             gCTGGGCAGCAACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTggc                                                                                                                     >  4:78131/1‑142 (MQ=255)                             gCTGGGCAGCAACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAg                                                                                                              >  1:126670/1‑149 (MQ=255)                               tGGGCAGCAACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAgg                                                                                                             >  7:71259/1‑148 (MQ=255)                                 ggCAGCAACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCAt                                                                                                          <  1:25499/149‑1 (MQ=255)                                  gcagcaACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCa                                                                                                           >  6:324031/1‑147 (MQ=255)                                   cagcaACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAg                                                                                                        <  5:324031/149‑1 (MQ=255)                                   cagcaACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAg                                                                                                        <  8:60998/149‑1 (MQ=255)                                     gcaACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACTGCGGCATTCCCGCTGTCTGccg                                                                                                                             <  7:272656/126‑2 (MQ=255)                                     gcaACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACTGCGGCATTCCCGCTGTCTGccg                                                                                                                             >  8:272656/1‑125 (MQ=255)                                     gcaACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGgc                                                                                                      >  6:114390/1‑149 (MQ=255)                                        aCTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCa                                                                                                   <  8:77206/149‑1 (MQ=255)                                         cTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTTATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAg                                                                                                  <  3:91343/149‑1 (MQ=255)                                            cAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTggcgg                                                                                                                   >  7:81069/1‑129 (MQ=255)                                            cAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTggcgg                                                                                                                   <  8:81069/129‑1 (MQ=255)                                            cAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAgg                                                                                                 <  7:25319/147‑1 (MQ=255)                                              ggggCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCAt                                                                                             <  5:114390/149‑1 (MQ=255)                                                 gCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATttc                                                                                          <  6:358712/149‑1 (MQ=255)                                                 gCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATttc                                                                                          >  5:358712/1‑149 (MQ=255)                                                 gCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATttc                                                                                          >  7:172631/1‑149 (MQ=255)                                                   aaaGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATttctt                                                                                        >  8:250423/1‑149 (MQ=255)                                                       ccAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCTTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATttcttcttc                                                                                    <  5:337591/149‑1 (MQ=255)                                                         agcagcagcCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAg                                                                                  >  5:233581/1‑149 (MQ=255)                                                         agcagcagcCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAg                                                                                  <  5:268963/149‑1 (MQ=255)                                                            agcagcCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGc                                                                                >  2:180698/1‑148 (MQ=255)                                                                gcCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCg                                                                           <  3:51849/149‑1 (MQ=255)                                                                 cccGGCCATGACGATGCTCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGGCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGGCATTTCTTCTTCAGGCAATCGc                                                                          <  6:379961/149‑1 (MQ=255)                                                                   cGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCt                                                                         >  2:177082/1‑148 (MQ=255)                                                                   cGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTc                                                                        <  8:71259/149‑1 (MQ=255)                                                                        aTGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATg                                                                     >  5:338643/1‑147 (MQ=255)                                                                               tgatCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTg                                                            <  6:309741/149‑1 (MQ=255)                                                                                gatCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTgc                                                           >  5:253014/1‑149 (MQ=255)                                                                                 atCAGGGTGGCCAGGGTGTTCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGTCAATCGCTCATGAAGGCCTTgcg                                                          <  4:280663/149‑1 (MQ=255)                                                                                      gCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCAt                                                     >  7:205003/1‑149 (MQ=255)                                                                                         ggCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATccc                                                  >  3:292402/1‑149 (MQ=255)                                                                                          gCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATcc                                                   <  8:205003/147‑1 (MQ=255)                                                                                          gCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATcc                                                   <  1:177082/147‑1 (MQ=255)                                                                                            cAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCt                                               >  3:211949/1‑149 (MQ=255)                                                                                              gggTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTc                                             <  4:236589/149‑1 (MQ=255)                                                                                                gTGATCTGACGGTGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGTGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCa                                            <  7:68661/148‑1 (MQ=255)                                                                                                 tGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAgg                                          >  3:194641/1‑149 (MQ=255)                                                                                                  gATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTgg                                                                                                                      <  5:70084/72‑1 (MQ=255)                                                                                                  gATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTgg                                                                                                                      >  6:70084/1‑72 (MQ=255)                                                                                                      tGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAg                                                                                                        <  8:21309/82‑1 (MQ=255)                                                                                                      tGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAg                                                                                                        >  7:21309/1‑82 (MQ=255)                                                                                                        aCGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTtgg                                   <  3:319129/149‑1 (MQ=255)                                                                                                           gggggTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTc                                                                        <  1:207573/109‑1 (MQ=255)                                                                                                           gggggTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTc                                                                        >  2:207573/1‑109 (MQ=255)                                                                                                                  aaGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTTGGTGGTGGCGt                          <  2:126670/148‑1 (MQ=255)                                                                                                                    ggTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTTGGTGGTGGCGTCAg                       <  7:250423/149‑1 (MQ=255)                                                                                                                     gTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTATTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTTGGTTGTGGCGTCAg                       >  5:85674/1‑148 (MQ=255)                                                                                                                      tCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTTGGTGGTGGCGTCAGCa                     <  2:141784/149‑1 (MQ=255)                                                                                                                        aTACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTtggtggt                               >  3:229739/1‑137 (MQ=255)                                                                                                                        aTACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTtggtggt                               <  4:229739/137‑1 (MQ=255)                                                                                                                        aTACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTTGGTGGTGGCGTCAGCAtt                   >  2:275458/1‑149 (MQ=255)                                                                                                                           cAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTTGGTGGTGGCGTCAGCATTGc                 <  6:106699/148‑1 (MQ=255)                                                                                                                              ccTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTTGGTGGTGGCGTCAGCCTTGCAGGt             >  7:290209/1‑149 (MQ=255)                                                                                                                               cTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTTGGTGGTGGCGTCAGCATTGCAGGTc            >  2:32880/1‑149 (MQ=255)                                                                                                                               cTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGCGCTTTGGTGGTGGCGTCAGCATTGCAGGTc            <  1:32880/149‑1 (MQ=255)                                                                                                                                tCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTTGGTGGTGGCGTCAGCATTGCAGGTCt           >  5:208178/1‑149 (MQ=255)                                                                                                                                  gctggCAGACTGCGGCATTCCCGCTGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCtt                                              <  6:11541/109‑1 (MQ=38)                                                                                                                                  gctggCAGACTGCGGCATTCCCGCTGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCtt                                              >  5:11541/4‑112 (MQ=38)                                                                                                                                  gACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTTGGTGGTGGCGTCAGCATTGCAGGTCTTc         <  6:338643/149‑1 (MQ=255)                                                                                                                                  gACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGCCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTTGGTGGTGGc                            >  5:24476/1‑130 (MQ=255)                                                                                                                                  gACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGCCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTTGGTGGTGGc                            <  6:24476/130‑1 (MQ=255)                                                                                                                                         gACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTTGGTGGTGGCGTCAGCATTGCAGGTCTTCAtttttt  <  4:292402/149‑1 (MQ=255)                                                                                                                                            aGCGGGAATGCCGCAGTCTGCGAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCTGGGCTTTGGTGGTGGCGTCAGCATTGCAGGTCTTCAttt     <  5:271635/143‑1 (MQ=255)                                                                                                                                            aGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCCTCCCCCTTCAGGCCTTTGGTGGTGGCGTCAGCATTGCAGGTCTTCAttt     >  6:271635/1‑143 (MQ=255)                                                                                                                                                     |                                                                                                                                         GTTCGAACACCAGCAGACCGGCCAGCAGGCTGGGCAGCAACTTCAGGGGCAAAGCCAGCAGCAGCCCGGCCATGACGATGATCAGGCTGGCCAGGGTGATCTGACGGGGGGTAAAGGTCATACAGCCTCGACGGCAGACAGCGGGAATGCCGCAGTCTGCCAGCCTTGGCGGCGCAGGCATAGGCGCAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTTGGTGGTGGCGTCAGCATTGCAGGTCTTCATTTTTT  >  NC_002947/5518400‑5518684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑