Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 478,988 G→A 6.0% R161C (CGC→TGC) ‡ cca ← multifunctional tRNA nucleotidyltransferase/2',3'‑cyclic phosphodiesterase/2' nucleotidase/phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947478,9880GA 6.0% 303.3 / 5.8 100R161C (CGC→TGC) ‡ccamultifunctional tRNA nucleotidyltransferase/2',3'‑cyclic phosphodiesterase/2' nucleotidase/phosphatase
Reads supporting (aligned to +/- strand):  ref base G (45/48);  new base A (3/3);  total (48/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.31e-01

Only 100 of 106 total aligned reads displayed.

GCTCAGGCAGCAGCTCCTGCAGGGCCCCACACGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACATGGCGTAGCAGGCGTTGATCCAGATCGTTCTTG  >  NC_002947/478841‑479133
                                                                                                                                                   |                                                                                                                                                   
gCTCAGGCAGCAGCTCCTGCAGGGCCCCACACGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGgcgc                                                                                                                                                    <  5:254526/149‑1 (MQ=255)
gCTCAGGCAGCAGCTCCTGCAGGGCCCCACACGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGgcg                                                                                                                                                     >  2:266735/1‑148 (MQ=255)
   cAGGCAGCAGCTCCTGCAGGGCCCCACACGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATc                                                                                                                                                 >  7:172651/1‑149 (MQ=255)
      gcagcaGCTCCTGCAGGGCCCCACACGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAgc                                                                                                                                              >  2:221542/1‑149 (MQ=255)
       cagcagCTCCTGCAGGGCCCCACACGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAgcg                                                                                                                                             <  8:281167/149‑1 (MQ=255)
        agcagcTCCTGCAGGGCCCCACACGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATGCGCCAGATCAgcgc                                                                                                                                            <  6:144909/149‑1 (MQ=255)
        agcagcTCCTGCAGGGCCCCACACGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATGCGCCAGATCAgcgc                                                                                                                                            <  8:133857/149‑1 (MQ=255)
        agcagcTCCTGCAGGGCCCCACACGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTCCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAgcgc                                                                                                                                            >  4:289845/1‑149 (MQ=255)
             cTCCTGCAGGGCCCCACACGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAgg                                                                                                                                       >  6:223021/1‑149 (MQ=255)
               ccTGCAGGGCCCCACACGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGtt                                                                                                                                     >  7:111461/1‑149 (MQ=255)
                cTGCAGGGCCCCACACGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGttt                                                                                                                                    <  5:21423/149‑1 (MQ=255)
                   cAGGGCCCCACACGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCAt                                                                                                                                 <  5:99618/149‑1 (MQ=255)
                      ggCCCCACACGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCaa                                                                                                                                         <  7:263217/138‑1 (MQ=255)
                      ggCCCCACACGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCaa                                                                                                                                         >  8:263217/1‑138 (MQ=255)
                      ggCCCCACACGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCgg                                                                                                                              >  4:258923/1‑149 (MQ=255)
                      ggCCCCACACGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCgg                                                                                                                              >  7:102055/1‑149 (MQ=255)
                      ggCCCCACACGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCgg                                                                                                                              <  3:285305/149‑1 (MQ=255)
                            acacGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAAccc                                                                                                                        >  6:154400/1‑149 (MQ=255)
                            acacGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAAccc                                                                                                                        >  3:161220/1‑149 (MQ=255)
                             cacGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCTCGCTCGATTTCATTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCTCCAAGGTTTCATCGGCAACCCg                                                                                                                       <  6:69568/149‑1 (MQ=255)
                                gcgcTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGaa                                                                                                                    <  2:183049/149‑1 (MQ=255)
                                gcgcTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGaa                                                                                                                    <  1:354290/149‑1 (MQ=255)
                                  gcTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAAcc                                                                                                                  >  2:28566/1‑149 (MQ=255)
                                      aGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAg                                                                                                              <  6:304916/149‑1 (MQ=255)
                                       gCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAgt                                                                                                             >  6:127689/1‑149 (MQ=255)
                                       gCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCACCTGCGCTCGGCAGTGAGGTCCTGCAGTTCGCCTGACGCGCTGATCTGGCGCATCAGCGCCAATGTTTCATCGGCAACCCGGAACCCCAgt                                                                                                             <  6:179445/149‑1 (MQ=255)
                                          ccTTGAAGAATAGCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAgtggt                                                                                                          <  3:289845/149‑1 (MQ=255)
                                            ttGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGc                                                                                                        >  4:168414/1‑149 (MQ=255)
                                             tGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCg                                                                                                       >  3:100701/1‑149 (MQ=255)
                                              gaagaaTACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGc                                                                                                        <  5:154400/147‑1 (MQ=255)
                                               aagaaTACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCACCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGt                                                                                                      >  3:177664/1‑148 (MQ=255)
                                                 gaaTACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAAc                                                                                                   >  5:90837/1‑149 (MQ=255)
                                                 gaaTACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAAc                                                                                                   >  1:73369/1‑149 (MQ=255)
                                                       cTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGgcag                                                                                             >  2:249955/1‑149 (MQ=255)
                                                       cTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGgcag                                                                                             >  6:107729/1‑149 (MQ=255)
                                                          cGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGgcagc                                                                                            <  2:146187/147‑1 (MQ=255)
                                                           ggTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCaaa                                                                                         <  7:189772/149‑1 (MQ=255)
                                                           ggTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCaaa                                                                                         <  3:168414/149‑1 (MQ=255)
                                                             ttGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGc                                                                                       <  3:328016/149‑1 (MQ=255)
                                                               gCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCg                                                                                      <  8:102055/148‑1 (MQ=255)
                                                                cACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑t                                                                                    <  4:207234/148‑1 (MQ=255)
                                                                cACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTCCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑t                                                                                    <  5:223021/148‑1 (MQ=255)
                                                                 aCTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAgtggt                                                                                                          <  6:9479/126‑1 (MQ=255)
                                                                 aCTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑Tg                                                                                   >  7:38739/1‑148 (MQ=255)
                                                                   ttCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCaa                                                                                >  5:170725/1‑149 (MQ=255)
                                                                   ttCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCaa                                                                                >  6:110840/1‑149 (MQ=255)
                                                                       aTCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAAcgcg                                                                            >  4:117160/1‑149 (MQ=255)
                                                                       aTCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAAcgcg                                                                            <  2:24056/149‑1 (MQ=255)
                                                                        tCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAAcgcg                                                                            >  1:91807/1‑148 (MQ=255)
                                                                          aGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCgcag                                                                         <  7:6377/149‑1 (MQ=255)
                                                                          aGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCgca                                                                          <  8:111461/148‑1 (MQ=255)
                                                                          aGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCACCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCgca                                                                          <  4:177664/148‑1 (MQ=255)
                                                                           gcgcgcgcTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCgcagc                                                                        >  3:13893/1‑149 (MQ=255)
                                                                              cgcgcTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCAcc                                                                     <  5:127689/149‑1 (MQ=255)
                                                                                  cTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGc                                                                  >  4:243131/1‑148 (MQ=255)
                                                                                   tCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCa                                                                 >  3:270848/1‑148 (MQ=255)
                                                                                     gATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCaaa                                                               <  1:249955/148‑1 (MQ=255)
                                                                                     gATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCaaa                                                               <  5:107729/148‑1 (MQ=255)
                                                                                      aTTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAgg                                                             <  4:161220/149‑1 (MQ=255)
                                                                                       atttcatccAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑a                                                           >  5:215735/7‑149 (MQ=255)
                                                                                        ttccttccAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑At                                                          >  3:102514/1‑149 (MQ=255)
                                                                                           cttccAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCt                                                        <  8:172651/148‑1 (MQ=255)
                                                                                           cttccAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCAGCAAAGG‑ATAtt                                                       >  4:97681/1‑149 (MQ=255)
                                                                                            ttccAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTc                                                      >  3:334684/1‑149 (MQ=255)
                                                                                            ttccAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTc                                                      >  7:103667/1‑149 (MQ=255)
                                                                                              ccAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCGCTGCAACGCGCAGCACCCGCAAAGG‑ATCtt                                                       <  1:28566/147‑1 (MQ=255)
                                                                                               cAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGc                                                                                                        <  8:199815/98‑1 (MQ=255)
                                                                                               cAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGc                                                                                                        >  7:199815/1‑98 (MQ=255)
                                                                                               cAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCCGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGc                                                   <  4:26165/149‑1 (MQ=255)
                                                                                                   tGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAAt                                               >  3:119484/1‑149 (MQ=255)
                                                                                                       cTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGCCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCg                                           <  1:317012/149‑1 (MQ=255)
                                                                                                        tCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCgg                                          >  6:256052/1‑149 (MQ=255)
                                                                                                          ggCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCgg                                          <  4:334684/147‑1 (MQ=255)
                                                                                                           gCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCgggg                                        >  3:38341/1‑148 (MQ=255)
                                                                                                              gtgagtgCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAAc                                    <  4:347236/149‑1 (MQ=255)
                                                                                                               tgagtgCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACa                                   >  1:63802/1‑149 (MQ=255)
                                                                                                                  gtgCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACATgg                                >  4:159887/1‑149 (MQ=255)
                                                                                                                  gtgCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACATgg                                <  3:117160/149‑1 (MQ=255)
                                                                                                                     ccTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑t                                                                                    >  8:5287/1‑95 (MQ=255)
                                                                                                                     ccTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGGAATCTTCGGCGAATGCCGGGGAAACATGGc                               <  1:221542/148‑1 (MQ=255)
                                                                                                                         cAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACATGGCGTAg                           <  6:90837/147‑1 (MQ=255)
                                                                                                                                 cTGAGGCTCTTATCTGGCTCATCAGCGCCAAGGTTTCATCGGCAAGCCGGAACCCCAGTGGTGCGTATCGGGCAGCAAAGAG‑TGCAACGCGCAGCACCCGCAAAGG‑ATGTTCTGCGAATTCCGGGGAAAAATGGCGTAGCATGCGTTGa                 <  6:215735/149‑1 (MQ=255)
                                                                                                                                 cTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACATGGCGTAGCAGGCGTTGa                 >  1:355294/1‑149 (MQ=255)
                                                                                                                                 cTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACATGGCGTAGCAGGCGTTGa                 <  2:63802/149‑1 (MQ=255)
                                                                                                                                   ttggCGCTGATGCGCCAGATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACATGGCGTAGCAGGCGt                    >  8:5733/3‑144 (MQ=255)
                                                                                                                                   ttggCGCTGATGCGCCAGATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACATGGCGTAGCAGGCGt                    <  7:5733/142‑1 (MQ=255)
                                                                                                                                   ttggCGCTGATGCGCCAGATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACATGGCGTAGCAGGCGTTGATc               >  8:115332/3‑149 (MQ=255)
                                                                                                                                   ttggCGCTGATGCGCCAGATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGAGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACATGGCGTAGCAGGCGTTGATc               >  5:227498/3‑149 (MQ=255)
                                                                                                                                    aGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACATGGCGTAGCAGGCGTTGATcc              <  7:59854/149‑1 (MQ=255)
                                                                                                                                    aGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACATGGCGTAGCAGGCGTTGATcc              <  3:258923/149‑1 (MQ=255)
                                                                                                                                    aGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACATGGCGTAGCAGGCGTTGATcc              <  4:119484/149‑1 (MQ=255)
                                                                                                                                     ggCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACATGGCGTAGCAGGCGTTGATc               >  8:54401/1‑147 (MQ=255)
                                                                                                                                       cgcTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACATGGCGTAGCAGGCGTTGATCCAGa           <  4:100701/149‑1 (MQ=255)
                                                                                                                                        gcTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACATGGCGTAGCAGGCGTTGATc               >  6:374261/1‑144 (MQ=255)
                                                                                                                                            aTCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACATGGCGTAGCAGGCGTTGATCCAGATCGtt      >  4:325473/1‑149 (MQ=255)
                                                                                                                                            aTCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACATGGCGTAGCAGGCGTTGATCCAGATCGtt      >  4:330845/1‑149 (MQ=255)
                                                                                                                                              cTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGTAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACATGGCGTAGCAGGCGTTGATCCAGATCGTTc     <  3:243131/148‑1 (MQ=255)
                                                                                                                                                 gcgcATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACATGGCGTAGCAGGCGTTGATCCAGATCGtt      <  4:38341/144‑1 (MQ=255)
                                                                                                                                                 gcgcATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACATGGCGTAGCAGGCGTTGATCCAGATCGtt      <  8:38739/144‑1 (MQ=255)
                                                                                                                                                 gcgcATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACATGGCGTAGCAGGCGTTGATCCAGATCGTTCtt   <  7:54401/147‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GCTCAGGCAGCAGCTCCTGCAGGGCCCCACACGCGCTCAGCACCTTGAAGAATACCTGCGGTTGCACTTCCATCAGCGCGCGCTCGATTTCCTTCCAGCTGCGCTCGGCAGTGAGTGCCTGCAGTTCGCCTGAGGCGCTGATCTGGCGCATCAGCGCCAAGGTTTCATCGGCAACCCGGAACCCCAGTGGTGCGTAACGGGCAGCAAAGCG‑TGCAACGCGCAGCACCCGCAAAGG‑ATCTTCGGCGAATGCCGGGGAAACATGGCGTAGCAGGCGTTGATCCAGATCGTTCTTG  >  NC_002947/478841‑479133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑