Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,538,577	G→T	9.1%	intergenic (+67/+54)	PP_4871 → / ← PP_4872	lysine decarboxylase family protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,538,577	0	G	T	9.1%	163.6 / 4.5	54	intergenic (+67/+54)	PP_4871/PP_4872	lysine decarboxylase family protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (26/23);  new base T (2/3);  total (28/26)
Fisher's exact test for biased strand distribution p-value = 6.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

GCAGCCGGACGAGCTGCTGGAAGGGATGGACAGCTTTGTGGCGCCGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGCTCGCCTCTTTGCGCTCCTTGGCAGCCTGCTGGTTACGC  >  NC_002947/5538429‑5538721                                                                                                                                                     |                                                                                                                                                 gcagcCGGACGAGCTGCTGGAAGGGATGGACAGCTTTGTGGCGCCGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATgg                                                                                                                                                  <  2:51221/149‑1 (MQ=255)     ccGGACGAGCTGCTGGAAGGGATGGACAGCTTTGTGGCGCCGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGa                                                                                                                                              <  7:269739/149‑1 (MQ=255)       ggACGAGCTGCTGGAAGGGATGGACAGCTTTGTGGCGCCGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGc                                                                                                                                            >  5:329098/1‑149 (MQ=255)       ggACGAGCTGCTGGAAGGGATGGACAGCTTTGTGGCGCCGGTCGCGCCTAAGTCGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGc                                                                                                                                            <  1:310453/149‑1 (MQ=255)         aCGAGCTGCTGGAAGGGATGGACAGCTTTGTGGCGCCGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCgg                                                                                                                                          <  2:66773/149‑1 (MQ=255)          cGAGCTGCTGGAAGGGATGGACAGCTTTGTGGCGCCGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCCCATTGCCTTTGTGGGAGCgg                                                                                                                                          <  4:44970/148‑1 (MQ=38)          cGAGCTGCTGGAAGGGATGGACAGCTTTGTGGCGCCGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGc                                                                                                                                         <  4:295413/149‑1 (MQ=255)          cGAGCTGCTGGAAGGGATGGACAGCTTTGTGGCGCCGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCCATCGCGACACAAGGCCGCTCCCCCATTGCCTTTGTGGGAGCGGc                                                                                                                                         <  6:187591/149‑1 (MQ=37)               tgctgGAAGGGATGGACAGCTTTGTGGCGCCGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTtgt                                                                                                                                    <  6:257209/149‑1 (MQ=255)                 ctgGAAGGGATGGACAGCTTTGTGGCGCCGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTAAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTtgtg                                                                                                                                   <  8:153913/148‑1 (MQ=255)                    gAAGGGATGGACAGCTTTGTGGCGCCGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTcg                                                                                                                                <  7:85772/148‑1 (MQ=255)                    gAAGGGATGGACAGCTTTGTGGCGCCGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTc                                                                                                                                 <  5:291742/147‑1 (MQ=255)                    gAAGGGATGGAAAGCTTTTTGGCTCCGGTCGCTCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTcgc                                                                                                                               <  8:352388/149‑1 (MQ=255)                        ggATGGACAGCTTTGTGGCGCCGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCCCATTGCCTTTGTGGGAGCGGCCTTGTGTCGCGaaa                                                                                                                           <  3:252913/149‑3 (MQ=37)                          aTGGACAGCTTTGTGGCGCCGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGAGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCGTGTGTCGCGATggg                                                                                                                         >  6:120825/1‑149 (MQ=255)                           tGGACAGCTTTGTGGCGCCGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTTGCAG‑CCCATCGCGACACAACGCCGCAACCCCATTGCCTTTGTGGGACCGGCCTTGTGTCGCGaaaggaa                                                                                                                       >  3:349295/1‑143 (MQ=17)                            ggACAGCTTTGTGGCGCCGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGGCTTGTGTCGCGCTGGGCt                                                                                                                       >  3:255069/1‑149 (MQ=255)                                aGCTTTGTGGCGCCGGTCGCGCGTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCATAAAGGCAATGGGGGAGCGGCCTTGTGGGGCGATGGGCTTCa                                                                                                                    >  7:235648/1‑148 (MQ=39)                                      ggggCCCCGGGCGCGCCTATGGGGGCGCACATCCCGCCTGAGTCACGCTGATACTTTTGGGTCCGTTGGGGGGTCCTTTTGCTACTCTATGCGTCTCCCATAATGTTATGGGGGTTGCGGCTTGGTGTCGCGATGGGCTGCAAagcag                                                                                                              <  8:235648/146‑1 (MQ=255)                                            ccGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCaa                                                                                                        >  3:225076/1‑148 (MQ=255)                                                                 gACAAGCGGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCGTGTGTCGCGATGGGCAGGAAAGCCGCCCCAATAGTCGCTGAGTTAAAGCGGaa                                                                                  >  8:297977/1‑149 (MQ=38)                                                                 gACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCCCGCCCACAAAGGGAAAGGGGGAGCGGGCGTGTGTCGCGATGGGCGGCAAAGCAGCCCCAAAAATAGATAAGTTAAAGCGGa                                                                                   >  4:213505/1‑148 (MQ=255)                                                                    aaGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGGAAAGCCGCCCCAATAGTCGATAAGTTAACGGGGAAtt                                                                                >  6:50295/1‑147 (MQ=255)                                                                      gcagcCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGa                                                                               >  5:89679/1‑147 (MQ=255)                                                                             gactgaAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGAc                                                                      >  4:351184/1‑149 (MQ=255)                                                                             gactgaAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGAc                                                                      >  1:221738/1‑149 (MQ=255)                                                                                      tGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGcttct                                                             >  5:281476/1‑149 (MQ=255)                                                                                       gagaCTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGcttctt                                                            >  3:313218/1‑149 (MQ=255)                                                                                         gaCTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCttg                                                           <  4:313218/148‑1 (MQ=255)                                                                                            ttttGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGctt                                                               <  7:40703/141‑1 (MQ=255)                                                                                            ttttGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGctt                                                               >  8:40703/1‑141 (MQ=255)                                                                                                  gACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGc                                                 >  6:304340/1‑149 (MQ=255)                                                                                                       cTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCAccc                                            >  8:195577/1‑149 (MQ=255)                                                                                                        ttCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTAGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGGTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTTCAACCCt                                           >  4:105410/1‑149 (MQ=255)                                                                                                          cgcgGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCt                                           <  3:351184/147‑1 (MQ=255)                                                                                                             ggTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGCTc                                      <  6:281476/149‑1 (MQ=255)                                                                                                                   ttCGCGACACAAGGCCGCTCCCACAACGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGCTCGCctct                                <  7:349454/149‑1 (MQ=255)                                                                                                                      gcgACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGCTCGCCTCTTTg                             >  6:332333/1‑149 (MQ=255)                                                                                                                         acacAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGCTCGCCTCTTTgcgc                          <  7:195577/149‑1 (MQ=255)                                                                                                                           acaAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCgg                                                                                    <  5:228514/89‑1 (MQ=255)                                                                                                                           acaAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCgg                                                                                    >  6:228514/1‑89 (MQ=255)                                                                                                                           acaAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCAccc                                            >  1:341189/1‑129 (MQ=255)                                                                                                                           acaAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCAccc                                            <  2:341189/129‑1 (MQ=255)                                                                                                                                gCCGCTCCCCCATTGCCTTTGTGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCAGACGCGGGTGCTTGTTGCCCGTGCCACACTGGATCGCGTCATGGCGCTCCTggg                   >  6:319209/1‑149 (MQ=21)                                                                                                                                 cctcTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGCTCGCCTCTTTGCGCTCCTTGGc                  <  4:225076/146‑1 (MQ=255)                                                                                                                                 ccGCTCCCACAAAGGCAATGGGGGAGCGGCCTGGTGTCGCGATGGGCTGCAAAGCAGCCCCAAGAGTCGAGCAGTTACCGCGGATTGTCAGGCTGACGCGGCTTCTGGTTGCCCTGGCCACCCTTGCTCGCCTCGTGGCGCTCCTTgga                  >  8:150084/1‑148 (MQ=38)                                                                                                                                   gCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGCTCGCCTCttt                              >  6:288333/1‑135 (MQ=255)                                                                                                                                   gCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGCTCGCCTCttt                              <  5:288333/135‑1 (MQ=255)                                                                                                                                    cTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGCTCGCCTCTTTGCGCTCCTTGGCAGc               >  5:114939/1‑149 (MQ=255)                                                                                                                                     tCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGCTCGCCTCTTTGCGCTCCTTGGCAGcc              >  3:230471/1‑149 (MQ=255)                                                                                                                                        cacaAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGCTCGCCTCTTTGCGCTCCTTGGCAGCctgc           <  6:89679/149‑1 (MQ=255)                                                                                                                                        cacaAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGCTCGCCTCTTTGCGCTCCTTGGCAGCctgc           >  3:191611/1‑149 (MQ=255)                                                                                                                                                 aaTGGGGGAGGGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGCTCGCCTCTTTGCGCTCCTTGGCAGCCTGCTGGTTAcgc  <  4:255069/149‑1 (MQ=255)                                                                                                                                                   tGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGCTCGCCTCTTTGCGCTCCTTgg                   >  4:220409/1‑130 (MQ=255)                                                                                                                                                   tGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGCTCGCCTCTTTGCGCTCCTTgg                   <  3:220409/130‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                 GCAGCCGGACGAGCTGCTGGAAGGGATGGACAGCTTTGTGGCGCCGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGCTCGCCTCTTTGCGCTCCTTGGCAGCCTGCTGGTTACGC  >  NC_002947/5538429‑5538721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑