Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,583,521 T→C 8.3% P737P (CCA→CCG)  parC ← DNA topoisomerase IV subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,583,5210TC 8.3% 165.9 / 4.8 60P737P (CCA→CCG) parCDNA topoisomerase IV subunit A
Reads supporting (aligned to +/- strand):  ref base T (28/27);  new base C (2/3);  total (30/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01

CCGGCACCGCAAGGGGCATATCATCCGCGAAATAACCGTCCGACTCCAGCATTTCTGCCCGAAACGGCGTTGAAATTGCCGTTCCAGACATTACAACCGGT‑TATGTCGGCACTTCCACCTGCAACCCATCGACGCGCTGGAACCCGCGTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGCCCTTGTAGTGCTCCAGGTCGTCCGCCTTCAAAGACAGGGTACGCTTGCCGGCCTGCAATACAAGGGTCGCGCCTTCGGGAATCACGGCCAGATCGGTAACAAATTCTTCACGGC  >  NC_002947/5583373‑5583666
                                                                                                                                                     |                                                                                                                                                 
ccGGCACCGCAAGGGGCATATCATCCGCGAAATAACCGTCCGACTCCAGCATTTCTGCCCGAAACGGCGTTGAAATTGCCGTTCCAGACATTACAACCGGT‑TATGTCGGCACTTCCACCTGCAACCCATCGACGCGCTGGAACCCGCGt                                                                                                                                                   <  2:243270/149‑1 (MQ=255)
       cGCAAGGGGCATATCATCCGCGAAATAACCGTCCGACTCCAGCATTTCTGCCCGAAACGGCGTTGAAATTGCCGTTCCAGACATTACAACCGGT‑TATGTCGGCACTTCCACCTGCAACCCATCGACGCGCTGGAACCCGCGTGGCAGc                                                                                                                                             >  7:212397/1‑148 (MQ=255)
        gCAAGGGGCATATCATCCGCGAAATAACCGTCCGACTCCAGCATTTCTGCCCGAAACGGCGTTGAAATTGCCGTTCCAGACATTACAACCGGT‑TATGTCGGCACTTCCACCTGCAACCCATCGACGCGCTGGAACCCGCGTGGCAGcaa                                                                                                                                           <  8:212397/149‑3 (MQ=255)
         cAAGGGGCATATCATCCGCGAAATAACCGACCGACTCCAGCATTTCTGCCCGAAACGGCGTTGAAATTGCCGTTCCAGACATTACAACCGGT‑TATGTCGGCACTTCCACCTGCAACACATCGACGCGCTGGAACCCGCGTGGCAGCtt                                                                                                                                           >  1:29541/1‑148 (MQ=255)
                cATATCATCCGCGAAATAACCGTCCGACTCCAGCATTTCTGCCCAAAACGGCGTTGAAATTGCCGTTCCAGACATTACAACCGGT‑TATGTCGGCACTTCCACCTGCAACCCATCGACGCGCTGGAACCCGCGTGGCAGCTTGCTGCcgc                                                                                                                                   <  8:38968/149‑1 (MQ=255)
                 atatCATCCGCGAAATAACCGTCCGACTCCAGCATTTCTGCCCGAAACGGCGTTGAAATTGCCGTTCCAGACATTACAACCGGT‑TATGTCGGCACTTCCACCTGCAACCCATCGACGCGCTGGAACCCGCGCGGCAGCAAGCTGCcacg                                                                                                                                  >  2:49220/1‑149 (MQ=255)
                 atatCATCCGCGAAATAACCGTCCGACTCCAGCATTTCTGCCCGAAACGGCGTTGAAATTGCCGTTCCAGACATTACAACCGGT‑TATGTCGGCACTTCCACCTGCAACCCATCGACGCGCTGGAACCCGCGCGGCAGCAAGCTGCcacg                                                                                                                                  >  2:43712/1‑149 (MQ=255)
                  tatCATCCGCGAAATAACCGTCCGACTCCAGCATTTCTGCCCGAAACGGCGTTGAAATTGCCGTTCCAGACATTACAACCGGT‑TATGTCGGCACTTCCACCTGCAACCCATCGACGCGCTGGAACCCGCGTGGCAGCTTGCTGCCgcgc                                                                                                                                 >  7:232147/1‑149 (MQ=255)
                  tatCATCCGCGAAATAACAGTCCGACTCCAGCATTTCTGCCCGAAACGGCGTTGAAATTGCCGTTCCAGACATTACAACCGGT‑TATGTCGGCACTTCCACCTGCAACCCATCGACGCGCTGGAACCCGCGCGGCAGCAAGCTGCCacgc                                                                                                                                 <  8:127021/149‑1 (MQ=255)
                    tcatcCGCGAAATAACCGTCCGACTCCAGCATTTCTGCCCGAAACGGCGTTGAAATTGCCGTTCCAGACATTACAACCGGT‑TATGTCGGCACTTCCACCTGCAACCCATCGACGCGCTGGAACCCGCGCGGCAGCAAGCTGCCacgcg                                                                                                                                <  1:43712/148‑2 (MQ=255)
                    tcatcCGCGAAATAACCGTCCGACTCCAGCATTTCTGCCCGAAACGGCGTTGAAATTGCCGTTCCAGACATTACAACCGGT‑TATGTCGGCACTTCCACCTGCAACCCATCGACGCGCTGGAACCCGCGCGGCAGCAAGCTGCCacgcg                                                                                                                                <  1:49220/148‑2 (MQ=255)
                     catcCGCGAAATAACCGTCCGACTCCAGCATTTCTGCCCGAAACGGCGTTGAAATTGCCGTTCCAGACATTACAACCGGT‑TATGTCGGCACTTCCACCTGCAACCCATCGACGCGCTGGAACCCGCGTGGCAGCTTGCTGCCGCGCCGa                                                                                                                              >  4:357335/1‑149 (MQ=255)
                       tcCCCGAAATAACCGTCCGACTCCAGCATTTCTGCCCGAAACGGCGTTGAAATTGCCGTTCCAGACATTACAACCGGT‑TATGTCGGCACTTCCACCTGCAACCCATCGACGCGCTGGAACCCACGTGGCAGCTTGCTGCCGCGCCGAcc                                                                                                                            <  6:6112/149‑1 (MQ=255)
                         cgcgAAATAACCGTCCGACTCCAGCATTTCTGCCCGAAACGGCGTTGAAATTGCCGTTCCAGACATTACAACCGGT‑TATGTCGGCACTTCCACCTGCAACCCATCGACGCGCTGGAACCCGCGTgg                                                                                                                                                 <  8:188594/126‑1 (MQ=255)
                         cgcgAAATAACCGTCCGACTCCAGCATTTCTGCCCGAAACGGCGTTGAAATTGCCGTTCCAGACATTACAACCGGT‑TATGTCGGCACTTCCACCTGCAACCCATCGACGCGCTGGAACCCGCGTgg                                                                                                                                                 >  7:188594/1‑126 (MQ=255)
                           cgAAATAACCGTCCGACTCCAGCATTTCTGCCCGAAACGGCGTTGAAATTGCCGTTCCAGACATTACAACCGGT‑TATGTCGGCACTTCCACCTGCAACCCATCGACGCGCTGGAACCCGCGTGGCAGCTTGCTGCCGCGCCGACCgcgc                                                                                                                        <  5:317080/149‑1 (MQ=255)
                               aTAACCGCCCGACTCCAGCATTTCTCCCCGAAACGGCGTTTAAATTGCCGTTCCAGACATTACAACCGGT‑TATGTCGGCACTTCCACCTGCAACCCATCGACGCGCTGGAACCCGCGTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGc                                                                                                                    <  2:358262/149‑1 (MQ=255)
                                    cGTCCGACTCCAGCCTTTCTGCCCGAAACGGCGTTGAAATTGCCGTTCCAGACATTACAACCGGT‑TATGTCGGCACTTCCACCTGCAACCCATCGACGCGCTGGAACCCGCGTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGCCCTTg                                                                                                               <  5:374130/149‑1 (MQ=255)
                                    cGTCCGACTCCAGCATTTCTGCCCGAAACGGCGTTGAAATTGCCGTTCCAGACATTACAACCGGTGAATGTCGGCACTTCCACCTGCAACCCATCGACGCGCTGGAACCCGCGTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGCCCtt                                                                                                                >  1:302713/1‑149 (MQ=255)
                                      tCCGACTCCAGCATTTCTGCCCGAAACGGCGTTGAAATTGCCGTTCCAGACATTACAACCGGT‑TATGTCGGCACTTCCACCTGCAACCCATCGACGCGCTGGAACCCGCGTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGCCCTTGTa                                                                                                             <  6:335855/149‑1 (MQ=255)
                                      tCCGACTCCAGCATTTCTGCCCGAAACGGCGTTGAAATTGCCGTTCCAGACATTACAACCGGT‑TATGTCGGCACTTCCACCTGCAACCCATCGACGCGCTGGAACCCGCGTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGCCCTTGTa                                                                                                             <  3:357335/149‑1 (MQ=255)
                                               aGCATTTCTGCCCGAAACGGCGTTGAAATTGCCGTTCCAGACATTACAACCGGT‑TATGTCGGCCCTTCCACCTGCAACCCATCGACGCGCTGGAACCCGCGTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGCCCTTGTAGTGCTCCAg                                                                                                    >  5:105045/1‑149 (MQ=255)
                                                 cATTTCTGCCCGAAACGGCGTTGAAATTGCCGTTCCAGACATTACAACCGGT‑TATGTAGGCACTTCCACCTGCAACCCATCGACGCGATGGAACCCGCGTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGCCCTTGTAGTGCTCCAGgt                                                                                                  >  4:322094/1‑149 (MQ=255)
                                                         cccGAAACGGCGTTGAAATTGCCGTTCCAGACATTACAACCGGT‑TATGTCGGCACTTCCACCTGCAACCCATCGACGCGCTGGAACCCGCGTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGCCCTTGTAGTGCTCCAGGTCGTcc                                                                                             <  6:5874/146‑1 (MQ=255)
                                                         cccGAAACGGAGTTGAAATTGCAGTTCCAGACATTACAACCGGT‑TATGTCGGCACTTCCACCTGCAACCCATCGACGCGCTGGAACCCGCGTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGCCCTTGTAGTGCTCCAGGTCGTCCGcc                                                                                          >  4:162036/1‑149 (MQ=255)
                                                          ccGAAACGGCGTTGAAATTGCCGTTCCAGACATTACAACCGGT‑TATGTCGGCACTTCCACCTGCAACCCATCGACGCGCTGGAACCCGCGTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGCCCTTGTAGTGCTCCAGGTCGTCCGCCt                                                                                         >  3:27037/1‑149 (MQ=255)
                                                           cGAAACGGCGTTGAAATTGCCGTTCCAGACATTACAACCGGT‑TATGTCGGCACTTCCACCTGCAACCCATCGACGCGCTGGAACCCGCGTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGCCCTTGTAGTGCTCCAGGTCGTCCGCCtt                                                                                        >  3:313221/1‑149 (MQ=255)
                                                                   cGTTGAAATTGCCGTTCCAGACATTACAACCGGT‑TATGTCGGCACTTCCACCTGCAACCCATCGACGCGCTGGAACCCGCGTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGCCCTTGTAGTGCTCCAGGTCGTCCGCCTTCAAAGACa                                                                                <  6:172668/149‑1 (MQ=255)
                                                                   cGTTGAAATTGCCGTTCCAGACATTACAACCGGT‑TATGTCGGCACTTCCACCTGCAACCCATCGACGCGCTGGAACCCGCGTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGCCCTTGTAGTGCTCCAGGTCGTCCGCCTTCAAAGACa                                                                                <  2:197054/149‑1 (MQ=255)
                                                                    gTTGAAATTGCCGTTCCAGACATTACAACCGGT‑TATGTTGGCACTTCCACCTGCAACCCATCGACGCGCTGGAACCCGCGTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGCCCTTGTAGTGCTCCAGGTCGTCCGCCTTCAAAGACAg                                                                               >  1:275039/1‑149 (MQ=255)
                                                                    gTTGAAATTGCCGTTCCAGACATTACAACCGGT‑TATGTCGGCACTTCCACCTGCAACCCATCGACGCGCTGGAACCCGCGTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGCCCTTGTAGTGCTCCAGGTCGTCCGCCTTCAAAGACAg                                                                               <  3:136740/149‑1 (MQ=255)
                                                                           ttGCCGTTCCAGACATTACAACCGGT‑TATGTCGGCACTTCCACCTGCAACCCATCGACGCGCTGGAACCCGCGTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGCCCTTGTAGTGCTCCAGGTCGTCCGCCTTCAAAGACAGGGTACGc                                                                        <  4:27037/149‑1 (MQ=255)
                                                                                   ccAGACATTACAACCGGT‑TATGTCGGCACTTCCACCTGCAACCCATCGACGCGCTGGAACCCGCGTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGCCCTTGTAGTGCTCCAGGTCGTCCGCCTTCAAAGACAGGGTACGCTTGCCGGc                                                                >  8:47576/1‑149 (MQ=255)
                                                                                            aCAACCGGT‑TATGTCGGCACTTCCACCTGCAACCCATCGACGCGCTGGAACCCGCGTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGCCCTTGTAGTGCTCCAGGTCGTCCGCCTTCAAAGACAGGGTACGCTTGCCGGCCTGCAATa                                                        >  1:86670/1‑148 (MQ=255)
                                                                                              aaCCGGT‑TATGTCGGCACTTCCACCTGCAACCCATCGACGCGCTGGAACCCGCGTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGCCCTTGTAGTGCTCCAGGTCGTCCGCCTTCAAAGACAGGGTACGCTTGCCGGCCTGCaa                                                          >  1:285571/1‑144 (MQ=255)
                                                                                              aaCCGGT‑TATGTCGGCACTTCCACCTGCAACCCATCGACGCGCTGGAACCCGCGTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGCCCTTGTAGTGCTCCAGGTCGTCCGCCTTCAAAGACAGGGTACGCTTGCCGGCCTGCaa                                                          <  2:285571/144‑1 (MQ=255)
                                                                                                       aTGTCGGCACTTCCACCTGCAACCCATCGACGCGCTGGAACCCGCGTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGCCCTTGTAGTGCTCCAGGTCGTCCGCCTTCAAAGACAGGGTACGCTTGCCGGCCTGCAATACAAGGGTcgc                                              <  4:313221/148‑1 (MQ=255)
                                                                                                         gTCGGCACTTCCACCTGCAACCCATCGACGCGCTGGAACCCGCGTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGCCCTTGTAGTGCTCCAGGTCGTCCGCCTTCAAAGACAGGGTACGCTTGCCGGCCTGCAATACAAGGGTCGCGcc                                           >  4:176621/1‑149 (MQ=255)
                                                                                                         gTCGGCACTTCCACCTGCAACCCATCGACGCGCTGGAACCCGCGTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGCCCTTGTAGTGCTCCAGGTCGTCCGCCTTCAAAGACAGGGTACGCTTGCCGGCCTGCAATACAAGGGTCGCGcc                                           >  6:248429/1‑149 (MQ=255)
                                                                                                         gTCGGCACTTCCACCTGCAACCCATCGACGCGCTGGAACCCGCGTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGCCCTTGTAGTCCTCCAGGTCGTCCGCCTTCAAAGACAGGGTACGCTTGCCGGCCTGCAATACAAGGGTCgcgc                                            >  6:258159/1‑148 (MQ=255)
                                                                                                          tCGGCACTTCCACCTGCAACCCATCGACGCGCTGGAACCCGCGTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGCCCTTGTAGTGCTCCAGGTCGTCCGCCTTCAAAGATAGGGTACGCTTGCCGGCCTGCAATACAAGGGTc                                                <  3:114903/143‑1 (MQ=255)
                                                                                                          tCGGCACTTCCACCTGCAACCCATCGACGCGCTGGAACCCGCGTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGCCCTTGTAGTGCTCCAGGTCGTCCGCCTTCAAAGAAAGGGTACGCTTGCCGGCCTGCAATACAAGGGTc                                                >  4:114903/1‑143 (MQ=255)
                                                                                                            ggCACTTCCACCTGCAACCCATCGACGCGCTGGAACCCGCGTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGCCCTTGTAGTGCTCCAGGTCGTCCGCCTTCAAAGACAGGGTACGCTTGCCGGCCTGCAATACAAGGGTCGCGCCTTc                                        >  5:375717/1‑149 (MQ=255)
                                                                                                             gCACTTCCACCTGCAACCCATCGAGGCGCTGGAACCCGCGTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGCCCTTGTAGTGCTCCAGGTCGTCCGCCTTCAAAGACAGGGTACGCTTGCCGGCCTGCAATACAAGGGTCGCGCCTTCg                                       <  2:29541/149‑1 (MQ=255)
                                                                                                              cACTTCCACCTGCAACCCATCGACGCGCTGGAACCCGCGTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGCCCTTGTAGTGCTCCAGGTCGTCCGCCTTCAAAGACAGGGTACGCTTGCCGGCCTGCAATACAAGGGTCGCGCCTTCgg                                      <  8:232147/149‑1 (MQ=255)
                                                                                                                  tCCACCTGCAACCCATCGACGCGCTGGAACCCGCGTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGCCCTTGTAGTGCTCCAGGTCGTCCGCCTTCAAAGACAGGGTACGCTTGTCGGCCTGCAATACAAGGGTCGCGCCTTCGGGAAt                                  >  4:219768/1‑149 (MQ=255)
                                                                                                                    cACCTGCAACCCATCGACGCGCTGGAACCCGCGTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGCCCTTGTAGTGCTCCAGGTCGTCCGCCTTCAAAGACAGGGTACGCTTGCCGGCCTGCAATACAAGGGTc                                                <  4:60182/133‑1 (MQ=255)
                                                                                                                    cACCTGCAACCCATCGACGCGCTGGAACCCGCGTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGCCCTTGTAGTGCTCCAGGTCGTCCGCCTTCAAAGACAGGGTACGCTTGCCGGCCTGCAATACAAGGGTc                                                >  3:60182/1‑133 (MQ=255)
                                                                                                                    cACCTGCAACCCATCGACGCGCTGGAACCCGCGTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGCCCTTGTAGTGCTCCAGGTCGTCCGCCTTCAAAGACAGGGTACGCTTGCCGGCCTGCAATACAAGGGTCGCGCCTTCGGGAATCa                                >  2:298735/1‑149 (MQ=255)
                                                                                                                       cTGCAACCCATCGACGCGCTGGAACCCGCGTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGCCCTTGTAGTGCTCCAGGTCGTCCGCCTTCAAAGACAGGGTACGCTTGCCGGCCTGCAATa                                                        <  7:210694/122‑1 (MQ=255)
                                                                                                                       cTGCAACCCATCGACGCGCTGGAACCCGCGTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGCCCTTGTAGTGCTCCAGGTCGTCCGCCTTCAAAGACAGGGTACGCTTGCCGGCCTGCAATa                                                        >  8:210694/1‑122 (MQ=255)
                                                                                                                         gCAACCCATCGACGCGCTGGAACCCGCGTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGCCCTTGTAGTGCTCCAGGTCGTCCGCCTTCAAAGACAGGGTACGCTTGCCGGCCTGCAATACAAGGGTCGCGCCTTCGGGAATCACGGc                            >  1:187275/1‑148 (MQ=255)
                                                                                                                             cccATCGACGCGCTGGAACCCGCGTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGCCCTTGTAGTGCTCCAGGTCGTCCGCCTTCAAAGACAGGGTACGCTTGCCGGCCTGCAATACAAGGGTCGCGCCTTCGGGAATCACGGCCAGAt                       <  6:105045/149‑1 (MQ=255)
                                                                                                                              ccATCGACGCGCTGGAACCCGCGTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGCCCTTGTAGTGCTCCAGGTCGTCCGCCTTCAAAGACAGGGTACGCTTGCCGGCCTGCAATACAAGGGTCGCGCCTTCGGGAATCACGGCCAGATc                      <  2:86670/149‑1 (MQ=255)
                                                                                                                              ccATCGACGCGCTGGAACCCGCGTGGCAGCTTGCTGCCGCGACGACCGCGCTCGCCCATGTAGTGCTCCAGGACGTCCGACTTCAAAGACAGGGTACGCTTGCCGGCCTGCAATACAAGGGTCGCGCCTTCGGGAATGACGGc                            >  7:317720/1‑143 (MQ=255)
                                                                                                                               cATCGACGCGCTGGAACCCGCGTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGCCCTTGTAGTGCTCCAGGTCGTCCGCCTTCAAAGACAGGGTACGCTTGCCGGCCTGCAATACAAGGGTCGCGCCTTCGGGAATCACGGCCAGATCg                     <  2:302713/149‑1 (MQ=255)
                                                                                                                                      gcgcTGGAACCCGCGTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGCCCTTGTAGTGCTCCAGGTCGTCCGCCTTCAAAGACAGGGTACGCTTGCCGGCCTGCAATACAAGGGTCGCGCCTTCGGGAATCACGGCCAGATCGGTAACaa              <  2:275039/149‑1 (MQ=255)
                                                                                                                                          tGGAACCCGCGTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGCCCTTGTAGTGCTCCAGGTCGTCCGCCTTCAAAGACAGGGTACGCTTGCCGGCCTGCAATACAAGGGTCGCGCCTTCGGGAATCACGGCCAGATCGGTAACAAAttc          >  8:332116/1‑149 (MQ=255)
                                                                                                                                              aCCCGCGTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGCCCTTGTAGTGCTCCAGGTCGTCCGCCTTCAAAGACAGGTTACGCTTGCCGGCCTGCAATACAAGGGTCGCGCCTTCGGGAATCACGGCCAGATCGGTAACAAATTCTTCa      <  3:322094/149‑1 (MQ=255)
                                                                                                                                              aCCCGCGTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGCCCTTGTAGTGCTCCAGGTCGTCCGCCTTCAAAGACAGGGTACGCTTGCCGGCCTGCAATACAAGGGTCGCGCCTTCGGGAATCACGGCCAGATCGGTAACAAATTCTTCa      <  1:298735/149‑1 (MQ=255)
                                                                                                                                                  gcgTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGCCCTTGTAGTGCTCCAGGTCGTCCGCCTTCAAAGACAGGGTACGCTTGCCGGCCTGCAATACAAGGGTCGCGCCTTCGGGAATCACGGCCAGATCGGTAACAAATTCTTCACGGc  >  7:209016/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
CCGGCACCGCAAGGGGCATATCATCCGCGAAATAACCGTCCGACTCCAGCATTTCTGCCCGAAACGGCGTTGAAATTGCCGTTCCAGACATTACAACCGGT‑TATGTCGGCACTTCCACCTGCAACCCATCGACGCGCTGGAACCCGCGTGGCAGCTTGCTGCCGCGCCGACCGCGCTCGCCCTTGTAGTGCTCCAGGTCGTCCGCCTTCAAAGACAGGGTACGCTTGCCGGCCTGCAATACAAGGGTCGCGCCTTCGGGAATCACGGCCAGATCGGTAACAAATTCTTCACGGC  >  NC_002947/5583373‑5583666

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑