Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,622,932 A→C 7.4% M269R (ATG→AGG) ‡ PP_4941 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,622,9320AC 7.4% 150.2 / 2.3 50M269R (ATG→AGG) ‡PP_4941hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (24/22);  new base C (2/2);  total (26/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGGCTCGCAGGTCGGCCAGCAGGTTGTGCCAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCATCTGCTGGTA  >  NC_002947/5622785‑5623080
                                                                                                                                                   |                                                                                                                                                    
aaGGCTCGCAGGTCGGCCAGCAGGTTGTGCCAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCAt                                                                                                                                                     <  1:19597/149‑1 (MQ=255)
   gCTCGCAGGTCGGCCAGCAGGTTGTGCCAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATggc                                                                                                                                                  <  4:61265/149‑1 (MQ=255)
       gCAGGTCGGCCAGCAGGTTGTGCCAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGTGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATggcaggc                                                                                                                                              >  2:378700/1‑149 (MQ=255)
            tCGGCCAGCAGGTTGTGCCAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCCATGCAGGCAGcac                                                                                                                                         <  8:92152/149‑3 (MQ=255)
            tCGGCCAGCAGGTTGTGCCAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCCATGCAGGCAGcac                                                                                                                                         <  7:141316/149‑3 (MQ=255)
                 cagcagGTTGTGCCAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACCCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTCCAgg                                                                                                                                     >  7:108590/1‑148 (MQ=255)
                       gTTGTGCCAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAg                                                                                                                               >  5:233322/1‑148 (MQ=255)
                          gtgCCAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAg                                                                                                                            <  5:86637/148‑1 (MQ=255)
                          gtgCCAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCCTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAg                                                                                                                            <  3:72238/148‑1 (MQ=255)
                               aGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGc                                                                                                                      >  6:191412/1‑149 (MQ=255)
                                    ggtgCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTAt                                                                                                                 <  5:327490/149‑1 (MQ=255)
                                      tgCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATg                                                                                                                >  8:335639/1‑148 (MQ=255)
                                       gcgcCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGc                                                                                                               >  1:140282/1‑148 (MQ=255)
                                                 ggTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTcc                                                                                                    <  7:335639/149‑1 (MQ=255)
                                                       ccGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACg                                                                                              >  5:149738/1‑149 (MQ=255)
                                                       ccGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACg                                                                                              >  8:310714/1‑149 (MQ=255)
                                                       ccGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACg                                                                                              >  8:164535/1‑149 (MQ=255)
                                                                   acgggTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAAcgc                                                                                  <  4:276229/149‑1 (MQ=255)
                                                                    cgggTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAAcgcg                                                                                 <  6:233322/149‑1 (MQ=255)
                                                                          gAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCacga                                                                           >  8:252505/1‑149 (MQ=255)
                                                                                gCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCGAACGCGGCACGACGATGc                                                                     >  2:293769/1‑149 (MQ=255)
                                                                                    aaCACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTc                                                                 >  5:354832/1‑149 (MQ=255)
                                                                                       acGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCa                                                              <  1:21288/149‑1 (MQ=255)
                                                                                               atCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACg                                                        >  7:223905/1‑147 (MQ=255)
                                                                                                    ccAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGgcgc                                                 <  7:252505/149‑1 (MQ=255)
                                                                                                      aGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGccc                                               <  1:378700/149‑1 (MQ=255)
                                                                                                           ggCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCa                                          <  7:232083/149‑1 (MQ=255)
                                                                                                            gCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAg                                         <  2:140282/149‑1 (MQ=255)
                                                                                                            gCTTCACGTGAGGCGGCCGGCTGGTCGGGCGATGCCAGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGGCCCAGGCACGCAGGCGCCCCTTCAg                                         <  5:112372/149‑1 (MQ=255)
                                                                                                              ttCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGgc                                       >  2:362561/1‑149 (MQ=255)
                                                                                                              ttCACGGGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGgc                                       <  8:108590/149‑1 (MQ=255)
                                                                                                                  cGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGgcgcgc                                   >  3:251831/1‑149 (MQ=255)
                                                                                                                   gTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATggca                                                                                                                                                 >  3:278657/1‑38 (MQ=255)
                                                                                                                   gTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATggca                                                                                                                                                 <  4:278657/38‑1 (MQ=255)
                                                                                                                    tGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGgcgc                                                 <  8:306519/133‑1 (MQ=255)
                                                                                                                    tGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGgcgc                                                 >  7:306519/1‑133 (MQ=255)
                                                                                                                      aGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGTTGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGcggcc                               >  5:318738/1‑149 (MQ=255)
                                                                                                                        gcggcCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGcggcctg                             >  2:119588/1‑148 (MQ=255)
                                                                                                                        gcggcCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGT‑CCAGGCACGCAGGCGCCCCTTCAGGCGCGcggcctcg                            <  6:59855/149‑1 (MQ=255)
                                                                                                                            ccggcTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAg                                         >  2:334191/1‑133 (MQ=255)
                                                                                                                            ccggcTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAg                                         <  1:334191/133‑1 (MQ=255)
                                                                                                                             cggcTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGcggcctcggcct                        >  6:201474/1‑149 (MQ=255)
                                                                                                                                   gTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCa                  <  6:149738/149‑1 (MQ=255)
                                                                                                                                   gTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCAGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGc                    <  7:164535/147‑1 (MQ=255)
                                                                                                                                     ctgcagctgcCTGCCATGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAgg                >  3:184081/6‑149 (MQ=255)
                                                                                                                                           ctgcctgcCATGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCATc          >  2:19105/1‑149 (MQ=255)
                                                                                                                                           ctgcctgcATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCATc          <  4:251831/149‑1 (MQ=255)
                                                                                                                                             gcctgcATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCATctg        >  8:228790/1‑149 (MQ=255)
                                                                                                                                              cctgcATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCATctgc       >  2:4651/1‑149 (MQ=255)
                                                                                                                                                tgcATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCATctgctg     >  7:144677/1‑149 (MQ=255)
                                                                                                                                                   aTGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCATCTGCTGGTa  <  5:191412/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
AAGGCTCGCAGGTCGGCCAGCAGGTTGTGCCAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCATCTGCTGGTA  >  NC_002947/5622785‑5623080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑