Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,623,395 G→T 6.4% R115R (CGG→AGG)  PP_4941 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,623,3950GT 6.4% 300.0 / 8.6 94R115R (CGG→AGG) PP_4941hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (43/44);  new base T (4/2);  total (48/46)
Fisher's exact test for biased strand distribution p-value = 6.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.19e-01

CCCTGCAGCCGGCAATGCCGGCCGCGCAGTCGCAACGTACCGGCCTGCAGTGGCGCAGCAAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAGGCTGGCAGGAACCTGCTGCACCTGATCGCCGAAGTCGTAGGCA  >  NC_002947/5623247‑5623543
                                                                                                                                                    |                                                                                                                                                    
cccTGCAGCCGGCAATGCCGGCCGCGCAGTCGCAACGTACCGGCCTGCAGTGGCGCAGCAAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCg                                                                                                                                                      >  4:109256/1‑149 (MQ=255)
 ccTGCAGCCGGCAATGCCGGCCGCGCAGTCGCAACGTACCGGCCTGCAGTGGCGCAGCAAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGa                                                                                                                                                     >  6:150326/1‑149 (MQ=255)
  cTGCAGCCGGCAATGCCGGCCGCGCAGTCGCAACGTACCGGCCTGCAGTGCCGCACCAAGACCCGTCAGGTTAAGGAAGCGCCCGCCATCGACGCCACGCTCGCACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCCAg                                                                                                                                                    >  2:370332/1‑149 (MQ=255)
         cGGCAATGCCGGCCGCGCAGTCGCAACGTACCGGCCTGCAGTGGCGCAGCAAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCTCGGGTCgag                                                                                                                                             <  3:140377/149‑1 (MQ=255)
         cGGCAATGCCGGCCGCGCAGTCGCAACGTACCGGCCTGCAGTGGCGCAGCAAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCgag                                                                                                                                             <  6:239447/149‑1 (MQ=255)
          ggCAATGCCGGCCGCGCAGTCGCAACGTACCGGCCTGCAGTGGCGCAGCAAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCTCGGGTCgagc                                                                                                                                            <  2:140846/149‑2 (MQ=255)
            cAATGCCGGCCGCGCAGTCGCAACGTACCGGCCTGCAGTGGCGCAGCAAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGaaa                                                                                                                                          <  2:282767/149‑1 (MQ=255)
            cAATGCCGGCCGCGCAGTCGCAACGTACCGGCCTGCAGTGGCGCAGCAAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGaaa                                                                                                                                          <  2:287664/149‑1 (MQ=255)
            cAATGCCGGCCGCGCAGTCGCAACGTACCGGCCTGCAGTGGCGCAGCAAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGaaa                                                                                                                                          <  5:128502/149‑1 (MQ=255)
             aaTGCCGGCCGCGCAGTCGCAACGTACCGGCCTGCAGTGGCGCAGCAAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAg                                                                                                                                         >  8:87498/1‑149 (MQ=255)
              aTGCCGGCCGCGCAGTCGCAACGTACCGGCCTGCAGTGGCGCAGCAAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAgc                                                                                                                                        >  3:187005/1‑149 (MQ=255)
                  cggccgCGCAGTCGCAACGTACCGGCCTGCAGTGGCGCAGCAAGACCCGTCAGGTTAAGGAAGCGCACGACATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGccc                                                                                                                                    <  2:265176/149‑1 (MQ=255)
                         gcAGTCGCAACGTACCGGCCTGCAGTGGCGCAGCAAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAg                                                                                                                             <  3:109256/149‑1 (MQ=255)
                                cAACGTACCGGCCTGCAGTGGCGCAGCAAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCttt                                                                                                                      <  5:150326/149‑1 (MQ=255)
                                cAACGTACCGGCCTGCAGTGGCGCAGCAAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCttt                                                                                                                      >  7:90387/1‑149 (MQ=255)
                                 aaCGTACCGGCCTGCAGTGGCGCAGCAAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCtttt                                                                                                                     >  4:273492/1‑149 (MQ=255)
                                 aaCGTACCGGCCTGCAGTGGCGCAGCAAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGATCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCtttt                                                                                                                     >  5:203235/1‑149 (MQ=255)
                                   cGTACCTGCCTGAAGTGGCGCAGGAAGACCAGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTa                                                                                                                   <  5:298307/149‑1 (MQ=255)
                                   cGTACCGGCCTGCAGTGGCGCAGCAAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTa                                                                                                                   <  4:84649/149‑1 (MQ=255)
                                    gTACCGGCCTGCAGTGGCGCAGCAAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGaaa                                                                                                                                          <  3:275178/125‑1 (MQ=255)
                                    gTACCGGCCTGCAGTGGCGCAGCAAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGaaa                                                                                                                                          >  4:275178/1‑125 (MQ=255)
                                     tACCGGCCTGCAGTGGCGCAGCAAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGc                                                                                                                 >  6:72522/1‑149 (MQ=255)
                                         ggCCTGCAGTGGCGCAGCAAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCACCAACCAGCTGCTTTTTAGCCGCa                                                                                                             <  1:2761/149‑1 (MQ=255)
                                              gCAGTGGCGCAGCAAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTc                                                                                                         >  4:348882/1‑148 (MQ=255)
                                               cAGTGGCGCAGCAAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCa                                                                                                        <  3:348882/148‑1 (MQ=255)
                                                aGTGGCGCAGCAAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAg                                                                                                      <  4:125124/149‑1 (MQ=255)
                                                      gcagcaAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGCGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAg                                                                                                >  8:65089/1‑149 (MQ=255)
                                                       cagcaAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAgg                                                                                               <  8:90387/149‑1 (MQ=255)
                                                         gcaAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCCACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGt                                                                                             >  8:348787/1‑149 (MQ=255)
                                                          caAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGtt                                                                                            <  6:152485/149‑1 (MQ=255)
                                                            aGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTtc                                                                                          >  6:23851/1‑149 (MQ=255)
                                                            aGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTtc                                                                                          <  7:240373/149‑1 (MQ=255)
                                                               cccGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTtcgtc                                                                                       >  1:36083/1‑149 (MQ=255)
                                                                ccGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGAAGTTCAAGCACAAGGGTTtcgtcg                                                                                      >  1:265011/1‑149 (MQ=255)
                                                                      ggTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTg                                                                                 >  1:193992/1‑148 (MQ=255)
                                                                       gTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTGTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGc                                                                                >  5:231810/1‑148 (MQ=255)
                                                                         tAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAAc                                                                             >  5:228730/1‑149 (MQ=255)
                                                                          aaGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACg                                                                            >  5:55021/1‑149 (MQ=255)
                                                                            ggAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGa                                                                                                                                                     >  7:67336/1‑74 (MQ=255)
                                                                            ggAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGa                                                                                                                                                     <  8:67336/74‑1 (MQ=255)
                                                                            ggAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTg                                                                          >  6:370064/1‑149 (MQ=255)
                                                                            ggAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTg                                                                          >  4:192422/1‑149 (MQ=255)
                                                                            ggAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTg                                                                          >  4:194081/1‑149 (MQ=255)
                                                                              aaGCGCACGCCATCGACGCCACGCTCGAACGCTTTCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGATAAAGCGCACCAACCAGATGCTTTTTAGCCGCAGTTAAACCACCACGGTATCGTCGCCTTGCAACGTGc                                                                         >  7:176766/1‑148 (MQ=255)
                                                                                 cgcACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATc                                                                     <  5:72522/149‑1 (MQ=255)
                                                                                  gcACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAAt                                                                      >  4:99731/1‑147 (MQ=255)
                                                                                  gcACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCa                                                                    <  3:192422/149‑1 (MQ=255)
                                                                                     cGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCg                                                                                                               >  6:319886/1‑103 (MQ=255)
                                                                                     cGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCg                                                                                                               <  5:319886/103‑1 (MQ=255)
                                                                                     cGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGcc                                                                 <  6:228730/149‑1 (MQ=255)
                                                                                      gCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTg                                                                          <  5:235486/139‑1 (MQ=255)
                                                                                      gCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTg                                                                          >  6:235486/1‑139 (MQ=255)
                                                                                       ccATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCtt                                                               <  7:87498/149‑1 (MQ=255)
                                                                                        cttCGACGCCACGCTCGAACGATTGCCGTTCGGCCGGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCG‑CCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGa                                                             <  6:249874/147‑1 (MQ=255)
                                                                                        cATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCtt                                                               >  6:245692/1‑148 (MQ=255)
                                                                                         aTCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGa                                                             <  6:282217/149‑1 (MQ=255)
                                                                                         aTCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGa                                                             <  4:187005/149‑1 (MQ=255)
                                                                                             aCGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGagcca                                                         <  7:15415/149‑1 (MQ=255)
                                                                                              cGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGagccag                                                        <  6:55021/149‑1 (MQ=255)
                                                                                               gCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGagccagc                                                       >  5:100456/1‑149 (MQ=255)
                                                                                                 cACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAg                                                                                                            <  5:125440/94‑1 (MQ=255)
                                                                                                 cACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAg                                                                                                            >  6:125440/1‑94 (MQ=255)
                                                                                                  aCGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCAt                                                    >  2:307029/1‑149 (MQ=255)
                                                                                                   cGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATc                                                   <  7:65089/149‑1 (MQ=255)
                                                                                                      tCGAACGCTTGCCGATCGGCCTGTTCGCCAGTCGGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATc                                                   <  7:21652/146‑1 (MQ=255)
                                                                                                      tCGAACGCTTGCCGATCGGCCTGTTCGCCAGTCGGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATc                                                   >  8:21652/1‑146 (MQ=255)
                                                                                                        gAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCAAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGAGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGc                                               <  2:76260/148‑1 (MQ=255)
                                                                                                          aCGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAgg                                            <  2:95016/149‑1 (MQ=255)
                                                                                                             cTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAGGCTg                                         >  7:9644/1‑149 (MQ=255)
                                                                                                             cTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAGGCTg                                         <  3:99731/149‑1 (MQ=255)
                                                                                                              ttGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAGGCTgg                                        >  5:341059/1‑149 (MQ=255)
                                                                                                                   gATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAGGCTGGCAGGa                                   <  5:245692/149‑1 (MQ=255)
                                                                                                                     tCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAGGCTGGCAGGAAc                                 <  4:284757/149‑1 (MQ=255)
                                                                                                                        gCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAGGCTGGCAGGAACctg                              >  8:288686/1‑149 (MQ=255)
                                                                                                                                 ccagccagCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGACAGCCATCGTGCAGGCTGGCAGGAACCTGCTGCACCt                      >  3:358938/1‑148 (MQ=255)
                                                                                                                                 ccagccagCAGCTCGAACCGACGGTAGAGAAAGAGCACCAACCATATCATATTTAGCCCCACATCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCATCCAACGTGCATCCTGGCACGAACCTTATGCACCt                      >  2:9087/1‑148 (MQ=255)
                                                                                                                                     ccagcAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAGGCTGGCAGGAACCTGCTGCACCTGATCg                 >  3:345652/1‑149 (MQ=255)
                                                                                                                                      cagcagCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAGGCTGGCAGGAACCTGCTGCACCTGATCGc                >  2:89645/1‑149 (MQ=255)
                                                                                                                                      cagcagCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAGGCTGGCAGGAACCTGCTGCACCTGATCGc                >  3:121052/1‑149 (MQ=255)
                                                                                                                                         tttcTCGACCCTCGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAGGCTGGCAGGAACCTGCTGCACCTGATCGCCg              >  1:338970/4‑148 (MQ=255)
                                                                                                                                          ttcTCGACCCTCGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAGGCTGGCAGGAACCTGCTGCACCTGATCGCCg              >  7:178188/3‑147 (MQ=255)
                                                                                                                                          agcTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAGGCTGGCAGGAACCTGCTGCACCTGATCGCCGaa            <  6:341059/149‑1 (MQ=255)
                                                                                                                                             tCGACCCTCGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAGGCTGGCAGGAACCTGCTGCACCTGATCGCCGAAGTc         >  1:265324/1‑149 (MQ=255)
                                                                                                                                             tCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGc                                                                                                              >  2:151679/1‑48 (MQ=255)
                                                                                                                                             tCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGc                                                                                                              <  1:151679/48‑1 (MQ=255)
                                                                                                                                               gACCCTCGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAGGCTGGCAGGAACCTGCTGCACCTGATCGCCGAAGTCg        >  7:61297/1‑148 (MQ=255)
                                                                                                                                               gACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAGGCTGGCAGGAACCTGCTGCACCTGATCGCCGAAGTc         <  8:9644/147‑1 (MQ=255)
                                                                                                                                               gACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAGGCTGGCAGGAACCTGCTGCACCTGATCGCCGAAGTc         <  5:23851/147‑1 (MQ=255)
                                                                                                                                               gACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAGGCTGGCAGGAACCTGCTGCACCTGATCGCCGAAGTCGt       >  7:338926/1‑149 (MQ=255)
                                                                                                                                               gACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAGGCTGGCAGGAACCTGCTGCACCTGATCGCCGAAGTCGt       <  3:273492/149‑1 (MQ=255)
                                                                                                                                                aCCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAGGCTGGCAGGAACCTGCTGCACCTGATCGCCGAAGTCGTa      <  6:203235/149‑1 (MQ=255)
                                                                                                                                                aCCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAGGCTGGCAGGAACCTGCTGCACCTGATCGCCGAAGTCGTa      <  6:100456/149‑1 (MQ=255)
                                                                                                                                                  ccGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAGGCTGGCAGGAACCTGCTGCACCTGATCGCCGAAGTc         <  2:254990/144‑1 (MQ=255)
                                                                                                                                                  ccGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAGGCTGGCAGGAACCTGCTGCACCTGATCGCCGAAGTc         >  1:254990/1‑144 (MQ=255)
                                                                                                                                                  ccGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAGGCTGGCAGGAACCTGCTGCACCTGATCGCCGAAGTCGTAgg    >  4:179152/1‑149 (MQ=255)
                                                                                                                                                    gAGGGTCGAGAAAGCGCCCCAACCATCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAGGCTGGCAGGAACCTGCTGCACCTGATCGcc               <  3:111081/136‑1 (MQ=255)
                                                                                                                                                    gAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAGGCTGGCAGGAACCTGCTGCACCTGATCGCCGAAGTCGTAGGCa  <  1:89645/149‑1 (MQ=255)
                                                                                                                                                    gAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAACCACCAGGGTTTCGTCGCCTTGCAACGTGAAATCAGCCTTGAGACAGACATCGTGCAGGCTGGCAGGAACCTCCTGCACCAGATCGcc               >  4:111081/1‑136 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CCCTGCAGCCGGCAATGCCGGCCGCGCAGTCGCAACGTACCGGCCTGCAGTGGCGCAGCAAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAGGCTGGCAGGAACCTGCTGCACCTGATCGCCGAAGTCGTAGGCA  >  NC_002947/5623247‑5623543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑