Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,629,625 G→T 12.6% A214D (GCT→GAT)  putP ← sodium/L‑proline transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,629,6250GT12.6% 135.4 / 3.9 50A214D (GCT→GAT) putPsodium/L‑proline transporter
Reads supporting (aligned to +/- strand):  ref base G (18/25);  new base T (5/2);  total (23/27)
Fisher's exact test for biased strand distribution p-value = 2.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTCTGGTCGAAGCCACCGGTGGAGATCAGCACGATTACCGGGGTCAGGATCAGCGCGAAGATCATCAGCGAAGCCTGTACGGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCG‑CCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAAGGTGCTCTCGAACAGACGGGCACCGGCCACGATGCCGGAGGCACAGTAGATGGTGAAGAACACCAGGATGACGATGGCGGAAATGATACGCAGCAGCCCGCTATTGTCTTC  >  NC_002947/5629477‑5629767
                                                                                                                                                     |                                                                                                                                              
ggTCTGGTCGAAGCCACCGGTGGAGATCAGCACGATTACCGGGGTCAGGATCAGCGCGAAGATCATCAGCGAAGCCTGTACGGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCG‑CCGCCGCACCAg                                                                                                                                                <  1:22473/149‑1 (MQ=255)
      gTCGAAGCCACCGGTGGAGATCAGCACGATTACCGGGGTCAGGATCAGCGCGAAGATCATCAGCGAAGCCTGTACGGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCG‑CCGCCGCACCAGCacac                                                                                                                                           >  3:114732/1‑148 (MQ=255)
         gAAGTCACCGGTGGAGATCAGCACGATTACCGGGGTCAGGATCAGCGCGAAGATCATCAGCGAAGCCTGTACGGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCG‑CCGCCGCACCAGCCCACaaa                                                                                                                                        <  2:145605/148‑1 (MQ=255)
              cACCGGTGGAGATCAGCACGATTACCGGGGTCAGGATCAGCGCGAAGATCATCAGCGAAGCCTGTACGGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCG‑CCGCCGCACCAGCCCACAAAGCGGtt                                                                                                                                  <  8:237012/149‑1 (MQ=255)
               aCCGGTTGAGATCAGCACGATTACCGGGGTCAGGATCAGCGCGAAGATCATCAGCGAAGCCTGTACGGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCG‑CCGCCGCACCAGCCCACAAAGCGGttt                                                                                                                                 <  5:91658/149‑1 (MQ=255)
               aCCGGTGGAGATCAGCACGATTACCGGGGTCAGGATCAGCGCGAAGATCATCAGCGAAGCCTGTACGGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCG‑CCGCCGCACCAGCCCACAAAGCGGttt                                                                                                                                 <  6:191048/149‑1 (MQ=255)
               aCCGGTGGAGATCAGCACGATTACCGGGGTCAGGATCAGCGCGAAGATCATCAGCGAAGCCTGTACGGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCG‑CCGCCGCACCAGCCCACAAAGCGGtt                                                                                                                                  >  6:22305/1‑148 (MQ=255)
                ccGGTGGAGATTAGCACTATTTCCGGTGTCAGGATCAGCGCGAAGATCATCAGCGAAGCCTGTACGTTATCGGTCCAGAGCACCGCCAGGAAGCCACCAACGAATGTGTAGGCGATGGTCG‑CCGCCGCACCAGCCCACAAAGCGGTTTc                                                                                                                                <  4:114732/149‑1 (MQ=255)
                    tGGAGATCAGCACGATTACCGGGGTCAGGATCAGCGCGAAGATCATCAGCGAAGCTTGTACTGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCG‑CCGCCGCACCAGCCCACAAAGCGGTTTCGTAc                                                                                                                            <  3:229111/149‑1 (MQ=255)
                        gaTCAGCACGATTACCGGGGTCAGGATCAGCGCGAAGATCATCAGCGAAGCCTGTACGGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGAGATGGTCG‑CCGCCGCACCAGACAACAAAGCGGTTTCGTACGACa                                                                                                                        >  2:169940/1‑149 (MQ=255)
                           cAGCACGATTACCGGGGTCAGGATCAGCGCGAAGATCATCAGCGAAGCCTGTACGGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCG‑CCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGc                                                                                                                     >  7:6575/1‑149 (MQ=255)
                               aCGATTACCGGGGTCAGGATCAGCGCGAAGATCATCAGCGAAGCCTGTACGGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCG‑CCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGaa                                                                                                                 <  7:96767/149‑1 (MQ=255)
                                cGATTACCGGGGTCAGGATCAGCGCGAAGATCATCAGCGAAGCCTGTACGGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCG‑CCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAAg                                                                                                                <  7:274071/149‑1 (MQ=255)
                                       cGGGGTCAGGATCAGCGCGAAGATCATCAGCGAAGCCTGTACGGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGACTAGGAGATCGTCG‑CCCCCGCACCATCACAAAAAAACGTTTACTCCGACATCCCTAAGGAGcact                                                                                                         >  8:276876/1‑149 (MQ=255)
                                                gATCAGCGCGAAGATCATCAGCGAAGCCTGTACGGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCG‑CCGCCGCACCAGCCCACAAAGCGGTTTCg                                                                                                                               <  2:211846/118‑1 (MQ=255)
                                                gATCAGCGCGAAGATCATCAGCGAAGCCTGTACGGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCG‑CCGCCGCACCAGCCCACAAAGCGGTTTCg                                                                                                                               >  1:211846/1‑118 (MQ=255)
                                                gATCAGCGCGAAGATCATCAGCGAAGCCTGTACGGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCG‑CCGCCGAACCAGACAACAAAGCGGTTTAGTACGACATGCCGAAGGTGCTCTCGAACAGAc                                                                                                >  8:305723/1‑149 (MQ=255)
                                                  tCAGCGAGAAGATCATCAGCGATTCCTTTACGGTATCTGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCG‑CCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAAGGTGCTCTCGAACAGACgg                                                                                              <  6:202515/149‑1 (MQ=255)
                                                       gcgAAGATCATCAGCGAAGCCTGTACGGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCG‑CCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAAGGTGCTCTCGAACAGACgg                                                                                              >  1:52496/1‑144 (MQ=255)
                                                       gcgAAGATCATCAGCGAAGCCTGTACGGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCG‑CCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAAGGTGCTCTCGAACAGACgg                                                                                              <  2:52496/144‑1 (MQ=255)
                                                        cgAAGATCATCAGCGAAGCCTGTACGGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGAACAAGGTCG‑ACGCCGAAACATAAAAAAAAAAAGTTTCGTACGACATGCCGAAGGTGCTCTCGAACAGACGGGCACCg                                                                                        >  4:271837/1‑149 (MQ=255)
                                                                 tcaGCGAAGCCTGTACGGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCG‑CCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAAGGTGCTCTCGAACAGACGGGCACCGGCCACGATg                                                                               >  1:372280/1‑149 (MQ=255)
                                                                      gAAGCCTGTACGGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCG‑CCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAAGGTGCTCTCGAACAGACGGGCACCGGCCACGATGCCGGa                                                                          <  5:287115/149‑1 (MQ=255)
                                                                       aaGCCTGTACGGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCG‑CCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAAGGTGCTCTCGAACAGACGGGCACCGGCCACGATGCCGGAg                                                                         <  2:342376/149‑1 (MQ=255)
                                                                       aaGCCTGTACGGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCG‑CCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAAGGTGCTCTCGAACAGACGGGCACCGGCCACGATGCCGGAg                                                                         <  7:305723/149‑1 (MQ=255)
                                                                         gCCTGTACGGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGGCG‑CCGCCGCACCAGCCCACAACGCGGTTTCGTACGACATGCCGAAGGTGCTCTCGAACAGACGGGCACCGGCCACGATGCCGGAGGc                                                                       <  3:113352/149‑1 (MQ=255)
                                                                          ccTGTACGGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCG‑CCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAAGGTGCTCTCGAACAGACGGGCACCGGCCACGATGCCGGAGGca                                                                      <  1:169940/149‑1 (MQ=255)
                                                                            tGTACGGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCG‑CCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAAGGTGCTCTCGAACAGACGGGCACCGGCCACGATGCCGGAGGca                                                                      >  3:256338/1‑147 (MQ=255)
                                                                            tGTACGGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCG‑CCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAAGGTGCTCTCGAACAGACGGGCACCGGCCACGATGCCGGAGGca                                                                      >  3:186119/1‑147 (MQ=255)
                                                                            tGTACGGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCG‑CCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAAGGTGCTCTCGAACAGACGGGCACCGGCCACGATGCCGGAGGca                                                                      >  1:5829/1‑147 (MQ=255)
                                                                              tACGGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCG‑CCTCCGCACCAGCCCACAAAGCGGTTTCGTACGACCTGCCGAAGGTGCTCTCGCACAGACGGGCACCGCACACGATGCCGGAGGCACAg                                                                   >  3:90925/1‑148 (MQ=255)
                                                                                  gTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCG‑CCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACGTGCCGAAGGTGCTCTCGAACAGACGGGCACCGGCCACGATGACGGAGGCACAGTAGAt                                                              >  3:41354/1‑149 (MQ=255)
                                                                                        gTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCG‑CCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAAGGTGCTCTCGAACAGACGGGCACCGGCCACGATGCCGGAGGCACAGTAGATGGTg                                                          >  1:12608/1‑147 (MQ=255)
                                                                                         tCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGAACAAGGTCG‑ACGCCGAAACATAAAAAAAAACAGTTTCGTACGACATGCCGAAGGTGCTCTCGAACAGACGGGCACCGGCCACGATGCCGGAGGCACAGTAGATGGTg                                                          >  6:90637/1‑146 (MQ=255)
                                                                                             gCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCGCCCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAAGGTGCTCTCGAACAGACGGGCACCGGCCACGATGCCGGAGGCACAGTAGATGGTgaagaa                                                     >  1:273701/1‑148 (MQ=255)
                                                                                               tCACCGCCAGGAAGCCACCAACGAAGGTGTAGAACAAGGTCG‑ACGCCGAAACATAAAAAAAAAAATTTTCGTACTACATGCCGAAGGTGATCTCGAACAGACGGGAACCGGCCACTATGCCGGAGTCACAGTAGATGGTGAAGAacac                                                  >  8:347525/1‑148 (MQ=255)
                                                                                                 aCCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCG‑CCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAAGGTGCTCTCGAACAGACGGGCACCGGCCACGATGCCGGAGGCACAGTAGATGGTGAAGAACACCa                                                <  1:269434/148‑1 (MQ=255)
                                                                                                       aGGAAGCCACCAACGAAGGTGTAGGCGATGGTCG‑CCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAAGGTGCTCTCGAACAGACGGGCACCGGCCACGATGCCGGAGGCACAGTAGATGGTGAAGAACACCAGGATGAc                                         <  3:271837/149‑1 (MQ=255)
                                                                                                         gAAGCCACCAACGAAGGTGTAGAACAAGGTCG‑ACGCCGAAACATAAAAAAAAAAAATTTCGTACGACATGCCGAAGGTGCTCTCGAACAGACGGGCACCGGCCACGATGCCGGAGGCACAGTAGATGGTGAAGAACACCAGGATGACGa                                       >  4:142428/1‑149 (MQ=255)
                                                                                                              caccaACGAAGGTGTAGGCGATGGTCG‑CCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAAGGTGCTCTCGAACAGACGGGCACCGGCCACGATGCCGGAGGCACAGTAGATGGTGAAGAACACCAGGATGa                                          >  1:234604/1‑141 (MQ=255)
                                                                                                              caccaACGAAGGTGTAGGCGATGGTCG‑CCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAAGGTGCTCTCGAACAGACGGGCACCGGCCACGATGCCGGAGGCACAGTAGATGGTGAAGAACACCAGGATGa                                          <  2:234604/141‑1 (MQ=255)
                                                                                                                 caACGAAGGTGTAGGCGATGGTCG‑CCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAAGGTGCTCTCGAACAGACGGGCACCGGCCACGATGCCGGAGGCACAGTAGATGGTGAAGAACACCAGGATGACGATGGCGGaa                               <  8:6575/149‑1 (MQ=255)
                                                                                                                  aaCGTAGGTGTAGGCGCTGGTCG‑GCGCCGCACCAGCCCACAATGCGGTTTCGTACGACATGCCGATGGTGCTCTGGAACTGACGGGCACCGGCCACGATGCCGGAGGCACAGTAGATGGTGAAGAACACCAGGATGACGATGGCGGaaa                              <  7:276876/149‑1 (MQ=255)
                                                                                                                   aCGAAGGTGTAGGCGATGGTCG‑CCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAAGGTGCTCTCGAACAGACGGGCACCGGCCACGATGCCGGAGGCACAGTAGATGGTGAAGAACACCAGGATGACGATGGCGGAAAt                             <  4:256338/149‑1 (MQ=255)
                                                                                                                    cGAAGGTGTAGGCGATGGTCG‑CCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAAGGTGCTCTCGAACAGACGGGCACCGGCCACGATGCCGGAGGCACAGTAGATGGTGAAGAACACCAGGATGACGATGGCGGAAAt                             >  7:296336/1‑148 (MQ=255)
                                                                                                                         gtgtAGTCGATGGTCG‑CCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAAGGTGCTCTCGAACAGACGGGCACCGGCCACGATGCCGGAGGCACAGTAGATGGTGAAGAACACCAGGATGACGATGGCGGAAATGATACg                       <  6:360549/149‑1 (MQ=255)
                                                                                                                            tAGGCGATGGTCG‑CCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAAGGTGCTCTCGAACAGACGGGCACCGGCCACGATGCCGGAGGCACAGTAGATGGTGAAGAACACCAGGATGACGATGGCGGAAATGATACgca                     <  2:372280/148‑1 (MQ=255)
                                                                                                                                        g‑ccgccgcACGATCCCACAAAGCGGTTTCGTCCGACATGCCGAAGGTTCTCTCGAACAGTCGGGCACCGGCCACGATGCCGGAGGCACAGTAGATGGTGAAGAACACCAGGATGACGATGGCGGAAATGATACGCAGCAGCCCGCTAtt        <  7:354161/149‑1 (MQ=255)
                                                                                                                                        g‑ccgccgcACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAAGGTGCTCTCGAACAGACGGGCACCGGCCACGATGCCGGAGGCACAGTAGATGGTGAAGAACACCAGGATGACGATGGCGGAAATGATACGCAGCAGCCCGCTAtt        >  8:277035/1‑149 (MQ=255)
                                                                                                                                             cagcACCATCCCAAAAAGCGGTTTCGTACGACATGCCGAAGGTGCTCTCGAACAGACGGGCAACGGCCACGATGGCGGAGGCACAGTAGATGGTGAAGAACACCAGGATGACGATGGCGGAAATGATACGCAGCAGGCCGCTATTGTCt    <  2:12608/147‑1 (MQ=255)
                                                                                                                                               gcACCAGCCCACAAAGCGGTTTCGTACGACCTGACGAAGGTGCTCTCGAACAGACGGGCACCGGCCACGATGCCGGAGGCACAGTAGATGGTGAAGAACACCAGGATGACGATGGCGGAAATGATACGCAGCAGCCCGCTATTGTCTTc  <  4:41354/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                              
GGTCTGGTCGAAGCCACCGGTGGAGATCAGCACGATTACCGGGGTCAGGATCAGCGCGAAGATCATCAGCGAAGCCTGTACGGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCG‑CCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAAGGTGCTCTCGAACAGACGGGCACCGGCCACGATGCCGGAGGCACAGTAGATGGTGAAGAACACCAGGATGACGATGGCGGAAATGATACGCAGCAGCCCGCTATTGTCTTC  >  NC_002947/5629477‑5629767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑