Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,641,185 G→C 7.1% G283A (GGC→GCC) ‡ PP_4951 → EmrB/QacA family drug resistance transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,641,1850GC 7.1% 217.3 / 4.0 70G283A (GGC→GCC) ‡PP_4951EmrB/QacA family drug resistance transporter
Reads supporting (aligned to +/- strand):  ref base G (32/32);  new base C (3/2);  total (35/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

TTGCTGCTGTTCGCCGGCATGGCCTGCCTGGCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGCCAAATCCATTGCCCGACCGCTGATCGAACGGTTCGGCTATC  >  NC_002947/5641037‑5641331
                                                                                                                                                    |                                                                                                                                                     
tagctgctGTTCGCCGGCATGGCCTGCCTGGCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCCAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAgcgg                                                                                                                                                       >  7:242005/3‑149 (MQ=255)
  gctgctGTTCGCCGGCATGGCCTGCCTGGCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAgcggcg                                                                                                                                                     >  6:50391/1‑149 (MQ=255)
     gctgTTCGCCGGCATGGCCTGCCTGGCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGgcgcgc                                                                                                                                                  >  1:156573/1‑149 (MQ=255)
     gctgTTCGCCGGCATGGCCTGCCTGGCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGACCGCCTGGGCAGCGgcgcgc                                                                                                                                                  >  7:276204/1‑149 (MQ=255)
       tgTTCGCCGGCATGGCCTGCCTGGCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTg                                                                                                                                                <  4:262086/149‑1 (MQ=255)
        gTTCGCCGGCATGGCCTGCCTGGCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGCGCCGCTGc                                                                                                                                               >  4:317658/1‑149 (MQ=255)
         ttCGCCGGCATGGCCTGCCTGGCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCGAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGcc                                                                                                                                              >  5:273993/1‑149 (MQ=255)
         ttCGCCGGCATGGCCTGCCTGGCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGcc                                                                                                                                              >  4:36340/1‑149 (MQ=255)
            gCCGGCATGGCCTGCCTGGCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGCGCCGCTGccca                                                                                                                                            <  3:317658/148‑3 (MQ=255)
              cGGCATGGCCTGCCTGGCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGCGCCGCTGccc                                                                                                                                             >  8:43014/1‑144 (MQ=255)
              cGGCATGGCCTGCCTGGCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGCGCCGCTGccc                                                                                                                                             <  7:43014/145‑2 (MQ=255)
                  aTGGCCTGCCTGGCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGt                                                                                                                                     <  5:243573/149‑1 (MQ=255)
                  aTGGCCTGCCTGGCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGt                                                                                                                                     >  7:326111/1‑149 (MQ=255)
                  aTGGCCTGCCTGGCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGt                                                                                                                                     <  3:298679/149‑1 (MQ=255)
                   tGGCCTGCCTGGCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTg                                                                                                                                    <  2:156573/149‑1 (MQ=255)
                    ggCCTGCCTGGCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGc                                                                                                                                   >  3:312495/1‑149 (MQ=255)
                      cctgcctgGCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCa                                                                                                                                 >  2:316792/1‑149 (MQ=255)
                          cctgGCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCAtt                                                                                                                               <  5:363251/147‑1 (MQ=255)
                           ctgGCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATtgct                                                                                                                            >  8:205468/1‑149 (MQ=255)
                           ctgGCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATtgct                                                                                                                            <  4:312495/149‑1 (MQ=255)
                           ctgGCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATtgct                                                                                                                            <  2:121322/149‑1 (MQ=255)
                              gCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATtgctgct                                                                                                                         >  1:336835/1‑149 (MQ=255)
                              gCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATtgctg                                                                                                                           <  5:352495/147‑1 (MQ=255)
                                cgcgTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATtgctgctgc                                                                                                                       <  3:172744/149‑1 (MQ=255)
                                cgcgTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATtgctgctgc                                                                                                                       <  3:178606/149‑1 (MQ=255)
                                 gcgTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATtgctgctg                                                                                                                        >  1:301188/1‑147 (MQ=255)
                                   gTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAgg                                                                                                                    <  2:180371/149‑1 (MQ=255)
                                    tACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGt                                                                                                                   <  4:137214/149‑1 (MQ=255)
                                     aCTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGtt                                                                                                                  >  1:284040/1‑149 (MQ=255)
                                         gCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTgcgc                                                                                                              >  2:104127/1‑149 (MQ=255)
                                         gCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTgcg                                                                                                               <  1:101946/148‑1 (MQ=255)
                                            gcgcgcCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTgg                                                                                                           <  2:284040/149‑1 (MQ=255)
                                             cgcgcCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGGTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTg                                                                                                            <  2:313518/147‑1 (MQ=255)
                                                gcCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTa                                                                                                       >  3:162782/1‑149 (MQ=255)
                                                  cGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACt                                                                                                     <  6:261546/149‑1 (MQ=255)
                                                      cgcgACCCGGAGCCGCTGTGCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCAcc                                                                                                 <  6:49456/149‑1 (MQ=255)
                                                      cgagACACGGAGCCGCGGTTCTCGCCAAGCCTGTTCCGCTTGCGTACTTTCGCCATAGGCATCCTGGGCAACCTGTTCGCCCGCCTTGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCAcc                                                                                                 <  6:321642/146‑1 (MQ=255)
                                                         gACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑c                                                                                             >  6:288183/1‑149 (MQ=255)
                                                          aCCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑cc                                                                                            <  4:353772/149‑1 (MQ=255)
                                                           cccGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGtt                                                                                                                                           >  7:34890/1‑102 (MQ=255)
                                                           cccGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGtt                                                                                                                                           <  8:34890/102‑1 (MQ=255)
                                                            ccGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑cc                                                                                            >  1:350062/1‑147 (MQ=255)
                                                                        ttCTCGCCAAGCCTGTTCCTCGTGCGTACTTTCGCGATCGTCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCTGCGCGCTGCCGTTCCTGGTGCCATTGCTTCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGgcatgag                                                                              <  3:66251/149‑1 (MQ=255)
                                                                             gCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGgcatgagcatga                                                                         >  2:352470/1‑149 (MQ=255)
                                                                               cAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATc                                                                       >  6:261450/1‑149 (MQ=255)
                                                                                aaGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTCGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGgcatgagcacctcgt                                                                      >  7:170425/1‑143 (MQ=255)
                                                                                       ttCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTgg‑‑c                                                             <  8:242005/149‑1 (MQ=255)
                                                                                                cgtACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCAGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTgg‑‑cggcggcggc                                                    <  1:316792/149‑1 (MQ=255)
                                                                                                 gtACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTgg‑‑cggcggcggc                                                    >  5:193187/1‑148 (MQ=255)
                                                                                                  tACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCa                                                  >  2:178655/1‑149 (MQ=255)
                                                                                                     tttCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGc                                               >  2:215921/1‑149 (MQ=255)
                                                                                                     tttCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCACGATCCCGCTGG‑‑CGGCGGCGGCCATGc                                               >  7:118186/1‑149 (MQ=255)
                                                                                                       tCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGACGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTAGGCTa                                                                                                       <  4:149349/94‑1 (MQ=255)
                                                                                                          ccATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCa                                                                                          <  5:198026/103‑1 (MQ=255)
                                                                                                          ccATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCa                                                                                          >  6:198026/1‑103 (MQ=255)
                                                                                                          ccATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGc                                           >  1:2137/1‑148 (MQ=255)
                                                                                                               ggCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGCCAAAt                                      <  5:288183/148‑1 (MQ=255)
                                                                                                                    ccTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGCCAAATCCATTg                                >  5:359087/1‑149 (MQ=255)
                                                                                                                     cTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCGCCCCTGGCCGGCATGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGCCAAATCCAtt                                 <  1:104127/148‑1 (MQ=255)
                                                                                                                     cTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGCCAAATCCATTg                                <  2:2137/148‑1 (MQ=255)
                                                                                                                     cTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGCCAAATCCATTGc                               >  1:145839/1‑149 (MQ=255)
                                                                                                                        ggCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGCCAAATCCATTGccc                             <  2:350062/148‑1 (MQ=255)
                                                                                                                         gCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGCCAAATCCATTGCCCGa                           >  3:95950/1‑149 (MQ=255)
                                                                                                                              cTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGCCAAATCCATTGCCCGACCGCt                      >  1:288008/1‑149 (MQ=255)
                                                                                                                              cTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGCCAAATCCATTGCCCGACCGCt                      >  5:252872/1‑149 (MQ=255)
                                                                                                                                   cgcccgccTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGCCAAATCCATTGCCCGACCGCTGATc                  >  5:347024/1‑148 (MQ=255)
                                                                                                                                    gcccgccTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGCCAAATCCATTGCCCGACCGCTGTTCg                 >  5:4897/1‑148 (MQ=255)
                                                                                                                                     cccgccTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGCCAAATCCATTGCCCGACCGCTGATCGaa               >  7:123767/1‑149 (MQ=255)
                                                                                                                                     cccgccTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGCCAAATCCATTGCCCGACCGCTGATCGaa               <  2:145839/149‑1 (MQ=255)
                                                                                                                                      ccgccTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGCCAAATCCATTGCCCGACCGCTGATCGAAc              >  6:44033/1‑149 (MQ=255)
                                                                                                                                      ccgccTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGCCAAATCCATTGCCCGACCGCTGATCGAAc              <  8:123767/149‑1 (MQ=255)
                                                                                                                                      ccgccTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCAGGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGCCAAATCCATTGCCCGACCGCTGATCGAAc              <  5:261450/149‑1 (MQ=255)
                                                                                                                                          aacggCAGCGCGCCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTAGTTAAGCGGCGGCCATGCTGGCCAAATCCATTGCCCGACCGCTGATCGAACgg            >  1:311469/4‑149 (MQ=255)
                                                                                                                                             ggCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCATGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGCCAAATCCATTGCCCGACCGCTGATCGAACGGTTCg        <  1:85390/148‑1 (MQ=255)
                                                                                                                                                  cggcgCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGCCAAATCCATTGCCCGACCGCTGATCGAACGGTTCGGCTATc  <  1:214490/149‑1 (MQ=255)
                                                                                                                                                  cggcgCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCACGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGCCAAATCCATTGCCCGACCGCTGATCGAACGGTTCGGCTATc  <  8:118186/149‑1 (MQ=255)
                                                                                                                                                   cgcgagcGGCCGGGCCTGGTGCCATTGCTGCCGCCTGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCCTGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGCCAAATCCATTGCCCGACCGCTGATCGAACGGTTCGGCTAt   <  8:276204/146‑1 (MQ=255)
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TTGCTGCTGTTCGCCGGCATGGCCTGCCTGGCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGCCAAATCCATTGCCCGACCGCTGATCGAACGGTTCGGCTATC  >  NC_002947/5641037‑5641331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑