Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,641,186 C→G 7.1% G283G (GGC→GGG) ‡ PP_4951 → EmrB/QacA family drug resistance transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,641,1860CG 7.1% 225.5 / 5.6 70G283G (GGC→GGG) ‡PP_4951EmrB/QacA family drug resistance transporter
Reads supporting (aligned to +/- strand):  ref base C (32/33);  new base G (3/2);  total (35/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

GCTGCTGTTCGCCGGCATGGCCTGCCTGGCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGCCAAATCCATTGCCCGACCGCTGATCGAACGGTTCGGCTATC  >  NC_002947/5641039‑5641331
                                                                                                                                                   |                                                                                                                                                    
gctgctGTTCGCCGGCATGGCCTGCCTGGCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAgcggcg                                                                                                                                                     >  6:50391/1‑149 (MQ=255)
   gctgTTCGCCGGCATGGCCTGCCTGGCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGgcgcgc                                                                                                                                                  >  1:156573/1‑149 (MQ=255)
   gctgTTCGCCGGCATGGCCTGCCTGGCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGACCGCCTGGGCAGCGgcgcgc                                                                                                                                                  >  7:276204/1‑149 (MQ=255)
     tgTTCGCCGGCATGGCCTGCCTGGCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTg                                                                                                                                                <  4:262086/149‑1 (MQ=255)
      gTTCGCCGGCATGGCCTGCCTGGCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGCGCCGCTGc                                                                                                                                               >  4:317658/1‑149 (MQ=255)
       ttCGCCGGCATGGCCTGCCTGGCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCGAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGcc                                                                                                                                              >  5:273993/1‑149 (MQ=255)
       ttCGCCGGCATGGCCTGCCTGGCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGcc                                                                                                                                              >  4:36340/1‑149 (MQ=255)
          gCCGGCATGGCCTGCCTGGCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGCGCCGCTGccca                                                                                                                                            <  3:317658/148‑3 (MQ=255)
            cGGCATGGCCTGCCTGGCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGCGCCGCTGccc                                                                                                                                             >  8:43014/1‑144 (MQ=255)
            cGGCATGGCCTGCCTGGCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGCGCCGCTGccc                                                                                                                                             <  7:43014/145‑2 (MQ=255)
                aTGGCCTGCCTGGCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGt                                                                                                                                     <  5:243573/149‑1 (MQ=255)
                aTGGCCTGCCTGGCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGt                                                                                                                                     >  7:326111/1‑149 (MQ=255)
                aTGGCCTGCCTGGCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGt                                                                                                                                     <  3:298679/149‑1 (MQ=255)
                 tGGCCTGCCTGGCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTg                                                                                                                                    <  2:156573/149‑1 (MQ=255)
                  ggCCTGCCTGGCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGc                                                                                                                                   >  3:312495/1‑149 (MQ=255)
                    cctgcctgGCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCa                                                                                                                                 >  2:316792/1‑149 (MQ=255)
                        cctgGCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCAtt                                                                                                                               <  5:363251/147‑1 (MQ=255)
                         ctgGCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATtgct                                                                                                                            >  8:205468/1‑149 (MQ=255)
                         ctgGCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATtgct                                                                                                                            <  4:312495/149‑1 (MQ=255)
                         ctgGCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATtgct                                                                                                                            <  2:121322/149‑1 (MQ=255)
                            gCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATtgctgct                                                                                                                         >  1:336835/1‑149 (MQ=255)
                            gCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATtgctg                                                                                                                           <  5:352495/147‑1 (MQ=255)
                              cgcgTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATtgctgctgc                                                                                                                       <  3:178606/149‑1 (MQ=255)
                              cgcgTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATtgctgctgc                                                                                                                       <  3:172744/149‑1 (MQ=255)
                               gcgTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATtgctgctg                                                                                                                        >  1:301188/1‑147 (MQ=255)
                                 gTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAgg                                                                                                                    <  2:180371/149‑1 (MQ=255)
                                  tACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGt                                                                                                                   <  4:137214/149‑1 (MQ=255)
                                   aCTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGtt                                                                                                                  >  1:284040/1‑149 (MQ=255)
                                       gCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTgcgc                                                                                                              >  2:104127/1‑149 (MQ=255)
                                       gCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTgcg                                                                                                               <  1:101946/148‑1 (MQ=255)
                                          gcgcgcCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTgg                                                                                                           <  2:284040/149‑1 (MQ=255)
                                           cgcgcCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGGTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTg                                                                                                            <  2:313518/147‑1 (MQ=255)
                                              gcCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTa                                                                                                       >  3:162782/1‑149 (MQ=255)
                                                cGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACt                                                                                                     <  6:261546/149‑1 (MQ=255)
                                                    cgcgACCCGGAGCCGCTGTGCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCAcc                                                                                                 <  6:49456/149‑1 (MQ=255)
                                                    cgagACACGGAGCCGCGGTTCTCGCCAAGCCTGTTCCGCTTGCGTACTTTCGCCATAGGCATCCTGGGCAACCTGTTCGCCCGCCTTGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCAcc                                                                                                 <  6:321642/146‑1 (MQ=255)
                                                       gACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑c                                                                                             >  6:288183/1‑149 (MQ=255)
                                                        aCCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑cc                                                                                            <  4:353772/149‑1 (MQ=255)
                                                         cccGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGtt                                                                                                                                           >  7:34890/1‑102 (MQ=255)
                                                         cccGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGtt                                                                                                                                           <  8:34890/102‑1 (MQ=255)
                                                          ccGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑cc                                                                                            >  1:350062/1‑147 (MQ=255)
                                                                      ttCTCGCCAAGCCTGTTCCTCGTGCGTACTTTCGCGATCGTCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCTGCGCGCTGCCGTTCCTGGTGCCATTGCTTCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGgcatgag                                                                              <  3:66251/149‑1 (MQ=255)
                                                                           gCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGgcatgagcatga                                                                         >  2:352470/1‑149 (MQ=255)
                                                                             cAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATc                                                                       >  6:261450/1‑149 (MQ=255)
                                                                              aaGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTCGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGgcatgagcacctcgt                                                                      >  7:170425/1‑143 (MQ=255)
                                                                                     ttCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTgg‑‑c                                                             <  8:242005/149‑1 (MQ=255)
                                                                                              cgtACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCAGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTgg‑‑cggcggcggc                                                    <  1:316792/149‑1 (MQ=255)
                                                                                               gtACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTgg‑‑cggcggcggc                                                    >  5:193187/1‑148 (MQ=255)
                                                                                                tACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCa                                                  >  2:178655/1‑149 (MQ=255)
                                                                                                   tttCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGc                                               >  2:215921/1‑149 (MQ=255)
                                                                                                   tttCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCACGATCCCGCTGG‑‑CGGCGGCGGCCATGc                                               >  7:118186/1‑149 (MQ=255)
                                                                                                     tCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGACGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTAGGCTa                                                                                                       <  4:149349/94‑1 (MQ=255)
                                                                                                        ccATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCa                                                                                          <  5:198026/103‑1 (MQ=255)
                                                                                                        ccATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCa                                                                                          >  6:198026/1‑103 (MQ=255)
                                                                                                        ccATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGc                                           >  1:2137/1‑148 (MQ=255)
                                                                                                             ggCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGCCAAAt                                      <  5:288183/148‑1 (MQ=255)
                                                                                                                  ccTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGCCAAATCCATTg                                >  5:359087/1‑149 (MQ=255)
                                                                                                                   cTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCGCCCCTGGCCGGCATGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGCCAAATCCAtt                                 <  1:104127/148‑1 (MQ=255)
                                                                                                                   cTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGCCAAATCCATTg                                <  2:2137/148‑1 (MQ=255)
                                                                                                                   cTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGCCAAATCCATTGc                               >  1:145839/1‑149 (MQ=255)
                                                                                                                      ggCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGCCAAATCCATTGccc                             <  2:350062/148‑1 (MQ=255)
                                                                                                                       gCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGCCAAATCCATTGCCCGa                           >  3:95950/1‑149 (MQ=255)
                                                                                                                            cTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGCCAAATCCATTGCCCGACCGCt                      >  5:252872/1‑149 (MQ=255)
                                                                                                                            cTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGCCAAATCCATTGCCCGACCGCt                      >  1:288008/1‑149 (MQ=255)
                                                                                                                                 cgcccgccTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGCCAAATCCATTGCCCGACCGCTGATc                  >  5:347024/1‑148 (MQ=255)
                                                                                                                                  gcccgccTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGCCAAATCCATTGCCCGACCGCTGTTCg                 >  5:4897/1‑148 (MQ=255)
                                                                                                                                   cccgccTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGCCAAATCCATTGCCCGACCGCTGATCGaa               >  7:123767/1‑149 (MQ=255)
                                                                                                                                   cccgccTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGCCAAATCCATTGCCCGACCGCTGATCGaa               <  2:145839/149‑1 (MQ=255)
                                                                                                                                    ccgccTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGCCAAATCCATTGCCCGACCGCTGATCGAAc              >  6:44033/1‑149 (MQ=255)
                                                                                                                                    ccgccTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGCCAAATCCATTGCCCGACCGCTGATCGAAc              <  8:123767/149‑1 (MQ=255)
                                                                                                                                    ccgccTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCAGGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGCCAAATCCATTGCCCGACCGCTGATCGAAc              <  5:261450/149‑1 (MQ=255)
                                                                                                                                        aacggCAGCGCGCCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTAGTTAAGCGGCGGCCATGCTGGCCAAATCCATTGCCCGACCGCTGATCGAACgg            >  1:311469/4‑149 (MQ=255)
                                                                                                                                           ggCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCATGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGCCAAATCCATTGCCCGACCGCTGATCGAACGGTTCg        <  1:85390/148‑1 (MQ=255)
                                                                                                                                                cggcgCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGCCAAATCCATTGCCCGACCGCTGATCGAACGGTTCGGCTATc  <  1:214490/149‑1 (MQ=255)
                                                                                                                                                cggcgCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCACGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGCCAAATCCATTGCCCGACCGCTGATCGAACGGTTCGGCTATc  <  8:118186/149‑1 (MQ=255)
                                                                                                                                                 cgcgagcGGCCGGGCCTGGTGCCATTGCTGCCGCCTGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCCTGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGCCAAATCCATTGCCCGACCGCTGATCGAACGGTTCGGCTAt   <  8:276204/146‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCTGCTGTTCGCCGGCATGGCCTGCCTGGCCGCGTACTGGCTGCGCGCCGGGCGCGACCCGGAGCCGCTGTTCTCGCCAAGCCTGTTCCGCGTGCGTACTTTCGCCATCGGCATCCTCGGCAACCTGTTCGCCCGCCTGGGCAGCGGCGCGCTGCCGTTCCTGGTGCCATTGCTGCTGCAGGTTGCGCTGGGCTACTCACCCG‑CCCAGGCCGGCATGAGCATGATCCCGCTGG‑‑CGGCGGCGGCCATGCTGGCCAAATCCATTGCCCGACCGCTGATCGAACGGTTCGGCTATC  >  NC_002947/5641039‑5641331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑