Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,683,417 T→A 6.3% Q1159L (CAA→CTA) ‡ PP_4988 ← signal transduction histidine kinase CheA/CheY‑like receiver

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,683,4170TA 6.3% 235.3 / 4.8 80Q1159L (CAA→CTA) ‡PP_4988signal transduction histidine kinase CheA/CheY‑like receiver
Reads supporting (aligned to +/- strand):  ref base T (34/41);  new base A (3/2);  total (37/43)
Fisher's exact test for biased strand distribution p-value = 6.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01

ATTGCCAACCACCAGTTCCACCTGCTTGCCCAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTGGGAGTGGCGGTCCATTTCCAGCGGGTCGAAGTCGTCATAGGCAT  >  NC_002947/5683269‑5683564
                                                                                                                                                    |                                                                                                                                                   
aTTGCCAACCACCAGTGCCACCTGCTAGCCCAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGtt                                                                                                                                                     <  8:129511/149‑1 (MQ=255)
 ttGGCAACGACCATGTCCACCTGCTTGCCCAGCTCGCTGGCCACTTGCCGCTCCACGCGCTGCAAGCGCGGCAACAGGCGCTCTAACGGCACAATCAGGGTGGCCGTAAGGAGCTCCTTCAACTGGCTGTTCACGCGCGCCTGCTGTTg                                                                                                                                                    <  8:177011/149‑1 (MQ=255)
      aaCCACCAGTTCCACCTGCTTGCCCAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTgcag                                                                                                                                                 >  3:72185/1‑147 (MQ=255)
          accaGTTCCACCTGCTTGCCCAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCt                                                                                                                                           >  5:70031/1‑149 (MQ=255)
          accaGTTCCACCTGCTTGCCCAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCt                                                                                                                                           >  3:159893/1‑149 (MQ=255)
          accaGTTCCACCTGCTTGCCCAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGCAACAGCAGGcg                                                                                                                                           <  1:51466/149‑2 (MQ=255)
           ccaGTTCCACCTGCTTGCCCAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTg                                                                                                                                          >  6:247240/1‑149 (MQ=255)
            caGTTCCACCTGCTTGCCCAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTg                                                                                                                                          >  5:23944/1‑148 (MQ=255)
            caGTTCCACCTGCTTGCCCAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGt                                                                                                                                         <  6:208443/149‑1 (MQ=255)
             aGTTCCACCTGCTTGCCCAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTa                                                                                                                                        <  5:212163/149‑1 (MQ=255)
                  cACCTGCTTGCCCAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGcct                                                                                                                                   >  5:304924/1‑149 (MQ=255)
                   aCCTGCTTGCCCAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGcctc                                                                                                                                  <  8:267764/149‑1 (MQ=255)
                   aCCTGCTTGCCCAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGcctc                                                                                                                                  <  4:201222/149‑1 (MQ=255)
                    ccTGCTTGCCCAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGcctcc                                                                                                                                 >  3:244721/1‑149 (MQ=255)
                    ccTGCTTGCCCAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGcctcc                                                                                                                                 <  5:211673/149‑1 (MQ=255)
                    ccTGCTTGCCCAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGcctcc                                                                                                                                 >  5:195071/1‑149 (MQ=255)
                     cTGCTTGCCCAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGcctcct                                                                                                                                >  5:287105/1‑149 (MQ=255)
                     cTGCTTGCCCAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGcctcct                                                                                                                                <  6:287105/149‑1 (MQ=255)
                         ttGCCCAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAgc                                                                                                                            >  2:344492/1‑149 (MQ=255)
                            cccAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAgcgcg                                                                                                                         >  4:226318/1‑149 (MQ=255)
                             ccAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAgcgcgc                                                                                                                        >  7:83563/1‑149 (MQ=255)
                             ccAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAgcgcgc                                                                                                                        >  7:52646/1‑149 (MQ=255)
                             ccAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAgcgcgc                                                                                                                        <  2:215732/149‑1 (MQ=255)
                             ccAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACACGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAgcgcgc                                                                                                                        >  1:105230/1‑149 (MQ=255)
                              cAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCt                                                                                                                       <  6:195071/149‑1 (MQ=255)
                              cAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCt                                                                                                                       >  6:254563/1‑149 (MQ=255)
                              cAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCt                                                                                                                       >  6:7212/1‑149 (MQ=255)
                                 cTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAg                                                                                                                    <  6:287236/149‑1 (MQ=255)
                                      tGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAgg                                                                                                               <  5:16964/149‑1 (MQ=255)
                                        gCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAggg                                                                                                              <  5:62043/148‑1 (MQ=255)
                                        gCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGt                                                                                                             <  3:226318/149‑1 (MQ=255)
                                         ccACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAggagg                                                                                                            <  7:3580/149‑4 (MQ=255)
                                         ccACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGtc                                                                                                            >  8:70542/1‑149 (MQ=255)
                                          cACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGtct                                                                                                           >  4:313168/1‑149 (MQ=255)
                                            cTTGCCGCACTACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGCTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTcc                                                                                                         <  6:8460/149‑1 (MQ=255)
                                               gCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCtt                                                                                                       >  4:144467/1‑148 (MQ=255)
                                                ccGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTc                                                                                                      <  8:316040/148‑1 (MQ=255)
                                                ccGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTc                                                                                                      <  8:83563/148‑1 (MQ=255)
                                                      cacGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTc                                                                                                      <  7:267273/142‑1 (MQ=255)
                                                      cacGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTc                                                                                                      >  8:267273/1‑142 (MQ=255)
                                                      cacGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTc                                                                                                 >  6:7851/1‑147 (MQ=255)
                                                      cacGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTc                                                                                                 >  5:214728/1‑147 (MQ=255)
                                                        cgcgCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGc                                                                                             <  6:70031/149‑1 (MQ=255)
                                                        cgcgCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGc                                                                                             <  6:23944/149‑1 (MQ=255)
                                                                  gcgcGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAgg                                                                                   <  3:144467/149‑1 (MQ=255)
                                                                  gcccgGCACCTGGCGCTCGAACGGCACCATCCGGGGGGCCGTCAGGCCCTCCTGCAACTGGGTGTTCACCCGCGCCTGCTGTTGCAGCTGGCTGTAGGCCTCCTCTgcgc                                                                                                                          <  4:349184/107‑1 (MQ=255)
                                                                    gcgGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGACCTCTTGCAACTGGCTGTTCACCCGCGCCTGCTGTTgc                                                                                                                                                   <  1:334729/83‑1 (MQ=255)
                                                                    gcgGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGACCTCATGCAACTGGCTGTTCACCCGCGCCTGCTGTTgc                                                                                                                                                   >  2:334729/1‑83 (MQ=255)
                                                                            aGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGaa                                                                         <  1:344492/149‑1 (MQ=255)
                                                                              gggcTCGCACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTGCACCCGCGCCTGCTGTTGCAGCAGGCTGTCGGCCGCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAaga                                                                       <  4:305404/147‑1 (MQ=255)
                                                                                gcTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAgg                                                                     <  5:7212/149‑1 (MQ=255)
                                                                                gcTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCCGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGTGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAgg                                                                     >  4:194891/1‑149 (MQ=255)
                                                                                     aaCGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCg                                                                <  5:254563/149‑1 (MQ=255)
                                                                                     aaCGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCg                                                                <  6:214728/149‑1 (MQ=255)
                                                                                      aCGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGt                                                               <  7:70542/149‑1 (MQ=255)
                                                                                        ggCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGa                                                             >  4:211735/1‑149 (MQ=255)
                                                                                           aCCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAg                                                          <  5:7851/149‑1 (MQ=255)
                                                                                                  gggTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGctgctg                                                    >  8:28318/1‑148 (MQ=255)
                                                                                                     tGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGc                                                >  3:327846/1‑149 (MQ=255)
                                                                                                        ccGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTgg                                             >  4:320453/1‑149 (MQ=255)
                                                                                                           tCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTGGGAg                                          <  3:320453/149‑1 (MQ=255)
                                                                                                              ggCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTGGGAGTgg                                       <  4:72185/149‑1 (MQ=255)
                                                                                                                     ctGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCATCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTGGGAGGGGCGGTCCa                                >  8:102770/1‑149 (MQ=255)
                                                                                                                              gCTGTTCACCCGCGACTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTGGGAGTGGCGGTCCATTTCCAGCg                       >  5:167513/1‑149 (MQ=255)
                                                                                                                               cTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTGGGAGTGGCGGTCCATTTCCAGCgg                      <  4:244721/149‑1 (MQ=255)
                                                                                                                                  ttCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAagag                                                                      <  3:28387/98‑1 (MQ=255)
                                                                                                                                  ttCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAagag                                                                      >  4:28387/1‑98 (MQ=255)
                                                                                                                                      ctacagcCTGCTGCAACAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTGGGAGTGGCGGTCCATTTCCAGCGGGTCGAAg               >  3:47792/6‑149 (MQ=255)
                                                                                                                                      ctacagcCTGCTGCAACAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTGGGAGTGGCGGTCCATTTCCAGCGGGTCGAAg               >  1:85272/6‑149 (MQ=255)
                                                                                                                                      cccGCGCCTGGTGTTGCTGCAGGCTGTAGGCGTCCTGAGCGCGCTGTGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCGGTGACAGCTGCTGCAGCTGGGAGTGGCGGTCCATTTCCAGCGGGTCGAAg               <  2:105230/149‑1 (MQ=255)
                                                                                                                                      cccGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTGGGAGTGGCGGTCCATTTCCAGCGGGTCGAAg               <  6:304924/149‑1 (MQ=255)
                                                                                                                                       ccGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTGGGAGTGGCGGTCCATTTCCAGCGGGTCGAAgt              >  2:358479/1‑149 (MQ=255)
                                                                                                                                       ccGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTGGGAGTGGCGGTCCATTTCCAGCGGGTCGAAgt              >  3:72911/1‑149 (MQ=255)
                                                                                                                                       ccGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTGGGAGTGGCGGTCCATTTCCAGCGGGTCGAAgt              >  3:2206/1‑149 (MQ=255)
                                                                                                                                        cgcgCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTGGGAGTGGCGGTCCATTTCCAGCGGGTCGAAgtc             <  5:247240/149‑1 (MQ=255)
                                                                                                                                        cgcgCCCGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGTGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTGGGAGTGGCGGTCCATTTCCAGCGGGTCGAAgtc             <  3:194891/149‑1 (MQ=255)
                                                                                                                                          cgcCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTGGGAGTGGCGGTCCATTTCCAGCGGGTCGAAgtcgt           <  4:2206/149‑1 (MQ=255)
                                                                                                                                            ccTGCTGCAACAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTGGGAGTgg                                       <  3:103739/119‑1 (MQ=255)
                                                                                                                                            ccTGCTGCAACAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTGGGAGTgg                                       >  4:103739/1‑119 (MQ=255)
                                                                                                                                             ctgctgTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTGGGAGTGGCGGTCCATTTCCAGCGGGTCGAAGTCGTCAt        <  3:211735/149‑1 (MQ=255)
                                                                                                                                                ctgTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTGGGAGTGGCGGTCCATTTCCAGCGGGTCGAAGTCGTCATAg      <  8:52646/148‑1 (MQ=255)
                                                                                                                                                   ttGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTGGGAGTGGCGGTCCATTTCCAGCGGGTCGAAGTCGTCATAGGCAt  >  8:75648/1‑149 (MQ=255)
                                                                                                                                                   ttGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTCGGAGTGGCGGTACATTTCCAGCGGGTCGAAGTCGTCAAAGGCa   >  8:50545/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ATTGCCAACCACCAGTTCCACCTGCTTGCCCAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTGGGAGTGGCGGTCCATTTCCAGCGGGTCGAAGTCGTCATAGGCAT  >  NC_002947/5683269‑5683564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑