Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,683,418 G→A 6.4% Q1159* (CAA→TAA) ‡ PP_4988 ← signal transduction histidine kinase CheA/CheY‑like receiver

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,683,4180GA 6.4% 235.5 / 4.4 78Q1159* (CAA→TAA) ‡PP_4988signal transduction histidine kinase CheA/CheY‑like receiver
Reads supporting (aligned to +/- strand):  ref base G (34/39);  new base A (3/2);  total (37/41)
Fisher's exact test for biased strand distribution p-value = 6.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01

TTGCCAACCACCAGTTCCACCTGCTTGCCCAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTGGGAGTGGCGGTCCATTTCCAGCGGGTCGAAGTCGTCATAGGCATCG  >  NC_002947/5683270‑5683566
                                                                                                                                                    |                                                                                                                                                    
ttGGCAACGACCATGTCCACCTGCTTGCCCAGCTCGCTGGCCACTTGCCGCTCCACGCGCTGCAAGCGCGGCAACAGGCGCTCTAACGGCACAATCAGGGTGGCCGTAAGGAGCTCCTTCAACTGGCTGTTCACGCGCGCCTGCTGTTg                                                                                                                                                      <  8:177011/149‑1 (MQ=255)
     aaCCACCAGTTCCACCTGCTTGCCCAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTgcag                                                                                                                                                   >  3:72185/1‑147 (MQ=255)
         accaGTTCCACCTGCTTGCCCAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCt                                                                                                                                             >  5:70031/1‑149 (MQ=255)
         accaGTTCCACCTGCTTGCCCAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCt                                                                                                                                             >  3:159893/1‑149 (MQ=255)
         accaGTTCCACCTGCTTGCCCAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGCAACAGCAGGcg                                                                                                                                             <  1:51466/149‑2 (MQ=255)
          ccaGTTCCACCTGCTTGCCCAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTg                                                                                                                                            >  6:247240/1‑149 (MQ=255)
           caGTTCCACCTGCTTGCCCAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTg                                                                                                                                            >  5:23944/1‑148 (MQ=255)
           caGTTCCACCTGCTTGCCCAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGt                                                                                                                                           <  6:208443/149‑1 (MQ=255)
            aGTTCCACCTGCTTGCCCAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTa                                                                                                                                          <  5:212163/149‑1 (MQ=255)
                 cACCTGCTTGCCCAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGcct                                                                                                                                     >  5:304924/1‑149 (MQ=255)
                  aCCTGCTTGCCCAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGcctc                                                                                                                                    <  8:267764/149‑1 (MQ=255)
                  aCCTGCTTGCCCAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGcctc                                                                                                                                    <  4:201222/149‑1 (MQ=255)
                   ccTGCTTGCCCAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGcctcc                                                                                                                                   >  5:195071/1‑149 (MQ=255)
                   ccTGCTTGCCCAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGcctcc                                                                                                                                   >  3:244721/1‑149 (MQ=255)
                   ccTGCTTGCCCAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGcctcc                                                                                                                                   <  5:211673/149‑1 (MQ=255)
                    cTGCTTGCCCAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGcctcct                                                                                                                                  >  5:287105/1‑149 (MQ=255)
                    cTGCTTGCCCAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGcctcct                                                                                                                                  <  6:287105/149‑1 (MQ=255)
                        ttGCCCAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAgc                                                                                                                              >  2:344492/1‑149 (MQ=255)
                           cccAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAgcgcg                                                                                                                           >  4:226318/1‑149 (MQ=255)
                            ccAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAgcgcgc                                                                                                                          >  7:52646/1‑149 (MQ=255)
                            ccAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAgcgcgc                                                                                                                          >  7:83563/1‑149 (MQ=255)
                            ccAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAgcgcgc                                                                                                                          <  2:215732/149‑1 (MQ=255)
                            ccAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACACGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAgcgcgc                                                                                                                          >  1:105230/1‑149 (MQ=255)
                             cAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCt                                                                                                                         <  6:195071/149‑1 (MQ=255)
                             cAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCt                                                                                                                         >  6:254563/1‑149 (MQ=255)
                             cAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCt                                                                                                                         >  6:7212/1‑149 (MQ=255)
                                cTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAg                                                                                                                      <  6:287236/149‑1 (MQ=255)
                                     tGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAgg                                                                                                                 <  5:16964/149‑1 (MQ=255)
                                       gCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAggg                                                                                                                <  5:62043/148‑1 (MQ=255)
                                       gCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGt                                                                                                               <  3:226318/149‑1 (MQ=255)
                                        ccACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAggagg                                                                                                              <  7:3580/149‑4 (MQ=255)
                                        ccACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGtc                                                                                                              >  8:70542/1‑149 (MQ=255)
                                         cACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGtct                                                                                                             >  4:313168/1‑149 (MQ=255)
                                           cTTGCCGCACTACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGCTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTcc                                                                                                           <  6:8460/149‑1 (MQ=255)
                                              gCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCtt                                                                                                         >  4:144467/1‑148 (MQ=255)
                                               ccGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTc                                                                                                        <  8:316040/148‑1 (MQ=255)
                                               ccGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTc                                                                                                        <  8:83563/148‑1 (MQ=255)
                                                     cacGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTc                                                                                                        >  8:267273/1‑142 (MQ=255)
                                                     cacGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTc                                                                                                        <  7:267273/142‑1 (MQ=255)
                                                     cacGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTc                                                                                                   >  5:214728/1‑147 (MQ=255)
                                                     cacGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTc                                                                                                   >  6:7851/1‑147 (MQ=255)
                                                       cgcgCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGc                                                                                               <  6:23944/149‑1 (MQ=255)
                                                       cgcgCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGc                                                                                               <  6:70031/149‑1 (MQ=255)
                                                                 gcgcGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAgg                                                                                     <  3:144467/149‑1 (MQ=255)
                                                                 gcccgGCACCTGGCGCTCGAACGGCACCATCCGGGGGGCCGTCAGGCCCTCCTGCAACTGGGTGTTCACCCGCGCCTGCTGTTGCAGCTGGCTGTAGGCCTCCTCTgcgc                                                                                                                            <  4:349184/107‑1 (MQ=255)
                                                                   gcgGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGACCTCTTGCAACTGGCTGTTCACCCGCGCCTGCTGTTgc                                                                                                                                                     <  1:334729/83‑1 (MQ=255)
                                                                   gcgGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGACCTCATGCAACTGGCTGTTCACCCGCGCCTGCTGTTgc                                                                                                                                                     >  2:334729/1‑83 (MQ=255)
                                                                           aGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGaa                                                                           <  1:344492/149‑1 (MQ=255)
                                                                             gggcTCGCACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTGCACCCGCGCCTGCTGTTGCAGCAGGCTGTCGGCCGCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAaga                                                                         <  4:305404/147‑1 (MQ=255)
                                                                               gcTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAgg                                                                       <  5:7212/149‑1 (MQ=255)
                                                                               gcTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCCGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGTGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAgg                                                                       >  4:194891/1‑149 (MQ=255)
                                                                                    aaCGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCg                                                                  <  6:214728/149‑1 (MQ=255)
                                                                                    aaCGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCg                                                                  <  5:254563/149‑1 (MQ=255)
                                                                                     aCGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGt                                                                 <  7:70542/149‑1 (MQ=255)
                                                                                       ggCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGa                                                               >  4:211735/1‑149 (MQ=255)
                                                                                          aCCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAg                                                            <  5:7851/149‑1 (MQ=255)
                                                                                                 gggTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGctgctg                                                      >  8:28318/1‑148 (MQ=255)
                                                                                                    tGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGc                                                  >  3:327846/1‑149 (MQ=255)
                                                                                                       ccGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTgg                                               >  4:320453/1‑149 (MQ=255)
                                                                                                          tCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTGGGAg                                            <  3:320453/149‑1 (MQ=255)
                                                                                                             ggCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTGGGAGTgg                                         <  4:72185/149‑1 (MQ=255)
                                                                                                                    ctGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCATCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTGGGAGGGGCGGTCCa                                  >  8:102770/1‑149 (MQ=255)
                                                                                                                             gCTGTTCACCCGCGACTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTGGGAGTGGCGGTCCATTTCCAGCg                         >  5:167513/1‑149 (MQ=255)
                                                                                                                              cTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTGGGAGTGGCGGTCCATTTCCAGCgg                        <  4:244721/149‑1 (MQ=255)
                                                                                                                                 ttCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAagag                                                                        <  3:28387/98‑1 (MQ=255)
                                                                                                                                 ttCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAagag                                                                        >  4:28387/1‑98 (MQ=255)
                                                                                                                                     ctacagcCTGCTGCAACAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTGGGAGTGGCGGTCCATTTCCAGCGGGTCGAAg                 >  3:47792/6‑149 (MQ=255)
                                                                                                                                     ctacagcCTGCTGCAACAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTGGGAGTGGCGGTCCATTTCCAGCGGGTCGAAg                 >  1:85272/6‑149 (MQ=255)
                                                                                                                                     cccGCGCCTGGTGTTGCTGCAGGCTGTAGGCGTCCTGAGCGCGCTGTGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCGGTGACAGCTGCTGCAGCTGGGAGTGGCGGTCCATTTCCAGCGGGTCGAAg                 <  2:105230/149‑1 (MQ=255)
                                                                                                                                     cccGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTGGGAGTGGCGGTCCATTTCCAGCGGGTCGAAg                 <  6:304924/149‑1 (MQ=255)
                                                                                                                                      ccGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTGGGAGTGGCGGTCCATTTCCAGCGGGTCGAAgt                >  3:2206/1‑149 (MQ=255)
                                                                                                                                      ccGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTGGGAGTGGCGGTCCATTTCCAGCGGGTCGAAgt                >  2:358479/1‑149 (MQ=255)
                                                                                                                                      ccGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTGGGAGTGGCGGTCCATTTCCAGCGGGTCGAAgt                >  3:72911/1‑149 (MQ=255)
                                                                                                                                       cgcgCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTGGGAGTGGCGGTCCATTTCCAGCGGGTCGAAgtc               <  5:247240/149‑1 (MQ=255)
                                                                                                                                       cgcgCCCGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGTGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTGGGAGTGGCGGTCCATTTCCAGCGGGTCGAAgtc               <  3:194891/149‑1 (MQ=255)
                                                                                                                                         cgcCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTGGGAGTGGCGGTCCATTTCCAGCGGGTCGAAgtcgt             <  4:2206/149‑1 (MQ=255)
                                                                                                                                           ccTGCTGCAACAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTGGGAGTgg                                         <  3:103739/119‑1 (MQ=255)
                                                                                                                                           ccTGCTGCAACAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTGGGAGTgg                                         >  4:103739/1‑119 (MQ=255)
                                                                                                                                            ctgctgTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTGGGAGTGGCGGTCCATTTCCAGCGGGTCGAAGTCGTCAt          <  3:211735/149‑1 (MQ=255)
                                                                                                                                               ctgTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTGGGAGTGGCGGTCCATTTCCAGCGGGTCGAAGTCGTCATAg        <  8:52646/148‑1 (MQ=255)
                                                                                                                                                  ttGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTGGGAGTGGCGGTCCATTTCCAGCGGGTCGAAGTCGTCATAGGCAt    >  8:75648/1‑149 (MQ=255)
                                                                                                                                                  ttGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTCGGAGTGGCGGTACATTTCCAGCGGGTCGAAGTCGTCAAAGGCa     >  8:50545/1‑148 (MQ=255)
                                                                                                                                                    gcagcaGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGTGGGCCCGTGACAGCTGCTGCAGCTGGGAGTGGCGGTCCATTTCCAGCGGGTCGAAGTCGTCATAGGCATCg  >  1:119828/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TTGCCAACCACCAGTTCCACCTGCTTGCCCAGCTCGCTGGCCACTTGCCGCACCACGCGCTGCAAGCGCGGCACCAGGCGCTCGAACGGCACCATCAGGGTGGCCGTCAGGCCCTCCTGCAACTGGCTGTTCACCCGCGCCTGCTGTTGCAGCAGGCTGTAGGCCTCCTGAGCGCGCTGAGCCAGGGTCTCCTTCAGGTCGAGCAGGTCCGAGGCAGACTCGAAGAGGGCCCGTGACAGCTGCTGCAGCTGGGAGTGGCGGTCCATTTCCAGCGGGTCGAAGTCGTCATAGGCATCG  >  NC_002947/5683270‑5683566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑