Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,684,809 T→A 6.5% D695V (GAC→GTC)  PP_4988 ← signal transduction histidine kinase CheA/CheY‑like receiver

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,684,8090TA 6.5% 180.3 / 2.9 62D695V (GAC→GTC) PP_4988signal transduction histidine kinase CheA/CheY‑like receiver
Reads supporting (aligned to +/- strand):  ref base T (28/30);  new base A (2/2);  total (30/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.47e-01

CGGGCTGTTGCTGCCAGCGCGACAACAACGTCTCTGCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGAACAACGACCGCGCAGCCTCCAGCCATTCCATTTCGGCCATTTGCAGTGGCAGTTGA  >  NC_002947/5684663‑5684955
                                                                                                                                                  |                                                                                                                                                  
cGGGCTGTTGCTGCCAGCGCGACAACAACGTCTCTGCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTc                                                                                                                                                   >  1:229314/1‑148 (MQ=255)
    cTGTTGCTGCCAGCGCGACAACAACGTCTCTGCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACgg                                                                                                                                              <  5:233182/149‑1 (MQ=255)
        tgctgcCAGCGCGACAACAACGTCTCTGCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCTGTCCACGGCCt                                                                                                                                           <  2:229314/148‑1 (MQ=255)
          ctgcCAGCGCGACAACAACGTCTCTGCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGTTGGCCAGGCCGTGGACCGCCGCCTGGc                                                                                                                                        <  2:246988/149‑1 (MQ=255)
           tgcCAGCGCGACAACAACGTCTCTGCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGc                                                                                                                                        >  5:33553/1‑148 (MQ=255)
            gcCAGCGCGACAACAACGTCTCTGCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGcc                                                                                                                                       >  1:213685/1‑148 (MQ=255)
               aGCGCGACAACAACGTCTCTGCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGTTGGCCAGGCCGTGGACCGCCGCCTGGCCAGcc                                                                                                                                   <  3:147937/149‑1 (MQ=38)
                gcgcGACAACAACGTCTCTGCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCt                                                                                                                                  >  6:225396/1‑149 (MQ=255)
                   cgACAACAACGTCTCTGCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCt                                                                                                                               >  4:16667/1‑149 (MQ=255)
                    gACAACAACGTCTCTGCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTCGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTc                                                                                                                              <  1:311179/149‑1 (MQ=255)
                      caacaaCGTCTCTGCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGa                                                                                                                            <  5:225396/149‑1 (MQ=255)
                       aacaagGTGTCTGCATCGCCCCGGATTGGCATGGCATGGGCCAGTAAGGTGGCCACCAACTGCGGATCCCGCTTGGTGGGGCGGGCATGGTGCGGGTATTCATGCAGTGGGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTctaa                                                                                                                           <  2:240462/149‑4 (MQ=255)
                       aacaacGTCTCTGCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGAt                                                                                                                           >  4:100458/1‑149 (MQ=255)
                       aacaacGTCTCTGCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGAt                                                                                                                           <  4:87236/149‑1 (MQ=255)
                        acaacGTCTCTGCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATc                                                                                                                          <  7:257195/149‑1 (MQ=255)
                          aacGTCTCTGCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAg                                                                                                                        >  1:96759/1‑149 (MQ=255)
                             gTCTCTGCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTc                                                                                                                     >  7:136776/1‑149 (MQ=255)
                                  tGCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCcgc                                                                                                                  <  4:81032/147‑1 (MQ=255)
                                  tGCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCcg                                                                                                                   <  2:155664/146‑1 (MQ=255)
                                  tGCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCgg                                                                                                                >  3:231937/1‑149 (MQ=255)
                                    cATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGAt                                                                                                                           >  2:87894/1‑136 (MQ=255)
                                    cATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGAt                                                                                                                           <  1:87894/136‑1 (MQ=255)
                                        gAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTg                                                                                                                                          >  7:75324/1‑117 (MQ=255)
                                        gAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTg                                                                                                                                          <  8:75324/117‑1 (MQ=255)
                                          gCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGGATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCggg                                                                                                         >  8:233181/1‑148 (MQ=255)
                                                 gTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCgggg                                                                                                        <  7:227584/142‑1 (MQ=255)
                                                 gTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCgggg                                                                                                        >  8:227584/1‑142 (MQ=255)
                                                    catggcatggGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATggcggc                                                                                                >  6:355433/1‑147 (MQ=255)
                                                    catggcatggGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATggcggc                                                                                                >  5:51989/1‑147 (MQ=255)
                                                    catggcatggGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATggcggc                                                                                                >  5:123447/1‑147 (MQ=255)
                                                      tggcatggGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAgg                                                                                            <  4:231937/149‑1 (MQ=255)
                                                        gcatggGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAgggg                                                                                          >  4:267720/1‑149 (MQ=255)
                                                        gcatggGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAggg                                                                                           <  7:87588/148‑1 (MQ=255)
                                                           tggGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGt                                                                                       <  7:233181/149‑1 (MQ=255)
                                                             ggCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGc                                                                                     <  7:9239/149‑1 (MQ=255)
                                                                   gAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGTTATc                                                                                 >  3:102415/1‑147 (MQ=255)
                                                                         ggCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTgcg                                                                         >  4:82073/1‑149 (MQ=255)
                                                                          gCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTgc                                                                          <  6:206893/147‑1 (MQ=255)
                                                                              ccAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAg                                                                    <  8:304598/149‑1 (MQ=255)
                                                                               cAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGAACCGGGGATGGAGGCCAGGGGCGTGCTATCGAGTAGCGCCAAga                                                                   >  8:260708/1‑148 (MQ=255)
                                                                                  cTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGcc                                                                  >  7:336418/1‑147 (MQ=255)
                                                                                  cTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCa                                                                <  3:100458/149‑1 (MQ=255)
                                                                                   tGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGcc                                                                  >  3:18566/1‑146 (MQ=255)
                                                                                     cGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGAt                                                             <  2:213685/149‑1 (MQ=255)
                                                                                         tCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGaa                                                         <  6:123447/149‑1 (MQ=255)
                                                                                           aCGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGAt                                                             <  5:206872/143‑1 (MQ=255)
                                                                                           aCGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGAt                                                             >  6:206872/1‑143 (MQ=255)
                                                                                           aCGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGACAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGaac                                                        >  7:90059/1‑148 (MQ=255)
                                                                                             gCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGaacaac                                                     <  3:16667/149‑1 (MQ=255)
                                                                                                 gctgcGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGAACAACGAc                                                  <  5:355433/148‑1 (MQ=255)
                                                                                                  ctgcGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGAACAACGACcg                                                >  3:55148/1‑149 (MQ=255)
                                                                                                  ctgcCGCCGGCATGGTGAGGGTCTTCATGCAGGCAGGCCAGGCGGCGGTCCACGCCCTGGCCAACCTGCTCGATCAGATCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGAACAACGACcg                                                >  2:8352/1‑149 (MQ=255)
                                                                                                      ggccggcATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGAACAACGACCGCGCa                                            <  3:267720/149‑1 (MQ=255)
                                                                                                                           cATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGAACAACGACCGCGCAGCCTCCAGCCATTCCATTTc                        <  8:336418/148‑1 (MQ=255)
                                                                                                                            aTGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGAACAACGACCGCGCAGCCTCCAGCCATTCCATTTCg                       <  2:96759/148‑1 (MQ=255)
                                                                                                                                aGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGAACAACGACCGCGCAGCCTCCAGCCATTCCATTTCGGCCAt                  <  2:266140/149‑1 (MQ=255)
                                                                                                                                 ggttGGCCAGGCCGTGGACCGCCGCCTGGCCAGCCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGAACAACGACCGCGCAGCCTCCAGCCATTCCATTTCGGCCAtt                 >  4:175373/4‑149 (MQ=38)
                                                                                                                                 ggCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGAACAACGACCGCGCAGCCTCCAGCCATTCCATTTCGGCCAtt                 >  2:378266/1‑149 (MQ=255)
                                                                                                                                  gttGGCCAGGCCGTGGACCGCCGCCTGGCCAGCCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGAACAACGACCGCGCAGCCTCCAGCCATTCCATTTCGGCCAttt                >  5:205239/3‑149 (MQ=39)
                                                                                                                                       ccAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGcc                                                                  <  7:54802/94‑1 (MQ=255)
                                                                                                                                       ccAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGcc                                                                  >  8:54802/1‑94 (MQ=255)
                                                                                                                                                ggTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGAACAACGACCGCGCAGCCTCCAGCCATTCCATTTCGGCCATTTGCAGTGGCAGTTGa  <  8:136776/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CGGGCTGTTGCTGCCAGCGCGACAACAACGTCTCTGCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGAACAACGACCGCGCAGCCTCCAGCCATTCCATTTCGGCCATTTGCAGTGGCAGTTGA  >  NC_002947/5684663‑5684955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑