Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,684,814 G→C 6.6% A693A (GCC→GCG)  PP_4988 ← signal transduction histidine kinase CheA/CheY‑like receiver

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,684,8140GC 6.6% 193.4 / 4.0 61A693A (GCC→GCG) PP_4988signal transduction histidine kinase CheA/CheY‑like receiver
Reads supporting (aligned to +/- strand):  ref base G (28/29);  new base C (2/2);  total (30/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.44e-01

CTGTTGCTGCCAGCGCGACAACAACGTCTCTGCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGAACAACGACCGCGCAGCCTCCAGCCATTCCATTTCGGCCATTTGCAGTGGCAGTTGATGG  >  NC_002947/5684667‑5684958
                                                                                                                                                   |                                                                                                                                                
cTGTTGCTGCCAGCGCGACAACAACGTCTCTGCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACgg                                                                                                                                                 <  5:233182/149‑1 (MQ=255)
    tgctgcCAGCGCGACAACAACGTCTCTGCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCTGTCCACGGCCt                                                                                                                                              <  2:229314/148‑1 (MQ=255)
      ctgcCAGCGCGACAACAACGTCTCTGCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGTTGGCCAGGCCGTGGACCGCCGCCTGGc                                                                                                                                           <  2:246988/149‑1 (MQ=255)
       tgcCAGCGCGACAACAACGTCTCTGCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGc                                                                                                                                           >  5:33553/1‑148 (MQ=255)
        gcCAGCGCGACAACAACGTCTCTGCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGcc                                                                                                                                          >  1:213685/1‑148 (MQ=255)
           aGCGCGACAACAACGTCTCTGCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGTTGGCCAGGCCGTGGACCGCCGCCTGGCCAGcc                                                                                                                                      <  3:147937/149‑1 (MQ=38)
            gcgcGACAACAACGTCTCTGCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCt                                                                                                                                     >  6:225396/1‑149 (MQ=255)
               cgACAACAACGTCTCTGCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCt                                                                                                                                  >  4:16667/1‑149 (MQ=255)
                gACAACAACGTCTCTGCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTCGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTc                                                                                                                                 <  1:311179/149‑1 (MQ=255)
                  caacaaCGTCTCTGCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGa                                                                                                                               <  5:225396/149‑1 (MQ=255)
                   aacaagGTGTCTGCATCGCCCCGGATTGGCATGGCATGGGCCAGTAAGGTGGCCACCAACTGCGGATCCCGCTTGGTGGGGCGGGCATGGTGCGGGTATTCATGCAGTGGGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTctaa                                                                                                                              <  2:240462/149‑4 (MQ=255)
                   aacaacGTCTCTGCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGAt                                                                                                                              >  4:100458/1‑149 (MQ=255)
                   aacaacGTCTCTGCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGAt                                                                                                                              <  4:87236/149‑1 (MQ=255)
                    acaacGTCTCTGCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATc                                                                                                                             <  7:257195/149‑1 (MQ=255)
                      aacGTCTCTGCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAg                                                                                                                           >  1:96759/1‑149 (MQ=255)
                         gTCTCTGCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTc                                                                                                                        >  7:136776/1‑149 (MQ=255)
                              tGCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCcgc                                                                                                                     <  4:81032/147‑1 (MQ=255)
                              tGCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCcg                                                                                                                      <  2:155664/146‑1 (MQ=255)
                              tGCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCgg                                                                                                                   >  3:231937/1‑149 (MQ=255)
                                cATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGAt                                                                                                                              >  2:87894/1‑136 (MQ=255)
                                cATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGAt                                                                                                                              <  1:87894/136‑1 (MQ=255)
                                    gAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTg                                                                                                                                             >  7:75324/1‑117 (MQ=255)
                                    gAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTg                                                                                                                                             <  8:75324/117‑1 (MQ=255)
                                      gCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGGATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCggg                                                                                                            >  8:233181/1‑148 (MQ=255)
                                             gTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCgggg                                                                                                           >  8:227584/1‑142 (MQ=255)
                                             gTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCgggg                                                                                                           <  7:227584/142‑1 (MQ=255)
                                                catggcatggGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATggcggc                                                                                                   >  6:355433/1‑147 (MQ=255)
                                                catggcatggGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATggcggc                                                                                                   >  5:123447/1‑147 (MQ=255)
                                                catggcatggGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATggcggc                                                                                                   >  5:51989/1‑147 (MQ=255)
                                                  tggcatggGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAgg                                                                                               <  4:231937/149‑1 (MQ=255)
                                                    gcatggGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAgggg                                                                                             >  4:267720/1‑149 (MQ=255)
                                                    gcatggGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAggg                                                                                              <  7:87588/148‑1 (MQ=255)
                                                       tggGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGt                                                                                          <  7:233181/149‑1 (MQ=255)
                                                         ggCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGc                                                                                        <  7:9239/149‑1 (MQ=255)
                                                               gAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGTTATc                                                                                    >  3:102415/1‑147 (MQ=255)
                                                                     ggCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTgcg                                                                            >  4:82073/1‑149 (MQ=255)
                                                                      gCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTgc                                                                             <  6:206893/147‑1 (MQ=255)
                                                                          ccAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAg                                                                       <  8:304598/149‑1 (MQ=255)
                                                                           cAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGAACCGGGGATGGAGGCCAGGGGCGTGCTATCGAGTAGCGCCAAga                                                                      >  8:260708/1‑148 (MQ=255)
                                                                              cTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGcc                                                                     >  7:336418/1‑147 (MQ=255)
                                                                              cTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCa                                                                   <  3:100458/149‑1 (MQ=255)
                                                                               tGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGcc                                                                     >  3:18566/1‑146 (MQ=255)
                                                                                 cGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGAt                                                                <  2:213685/149‑1 (MQ=255)
                                                                                     tCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGaa                                                            <  6:123447/149‑1 (MQ=255)
                                                                                       aCGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGAt                                                                >  6:206872/1‑143 (MQ=255)
                                                                                       aCGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGAt                                                                <  5:206872/143‑1 (MQ=255)
                                                                                       aCGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGACAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGaac                                                           >  7:90059/1‑148 (MQ=255)
                                                                                         gCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGaacaac                                                        <  3:16667/149‑1 (MQ=255)
                                                                                             gctgcGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGAACAACGAc                                                     <  5:355433/148‑1 (MQ=255)
                                                                                              ctgcGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGAACAACGACcg                                                   >  3:55148/1‑149 (MQ=255)
                                                                                              ctgcCGCCGGCATGGTGAGGGTCTTCATGCAGGCAGGCCAGGCGGCGGTCCACGCCCTGGCCAACCTGCTCGATCAGATCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGAACAACGACcg                                                   >  2:8352/1‑149 (MQ=255)
                                                                                                  ggccggcATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGAACAACGACCGCGCa                                               <  3:267720/149‑1 (MQ=255)
                                                                                                                       cATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGAACAACGACCGCGCAGCCTCCAGCCATTCCATTTc                           <  8:336418/148‑1 (MQ=255)
                                                                                                                        aTGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGAACAACGACCGCGCAGCCTCCAGCCATTCCATTTCg                          <  2:96759/148‑1 (MQ=255)
                                                                                                                            aGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGAACAACGACCGCGCAGCCTCCAGCCATTCCATTTCGGCCAt                     <  2:266140/149‑1 (MQ=255)
                                                                                                                             ggttGGCCAGGCCGTGGACCGCCGCCTGGCCAGCCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGAACAACGACCGCGCAGCCTCCAGCCATTCCATTTCGGCCAtt                    >  4:175373/4‑149 (MQ=38)
                                                                                                                             ggCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGAACAACGACCGCGCAGCCTCCAGCCATTCCATTTCGGCCAtt                    >  2:378266/1‑149 (MQ=255)
                                                                                                                              gttGGCCAGGCCGTGGACCGCCGCCTGGCCAGCCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGAACAACGACCGCGCAGCCTCCAGCCATTCCATTTCGGCCAttt                   >  5:205239/3‑149 (MQ=39)
                                                                                                                                   ccAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGcc                                                                     <  7:54802/94‑1 (MQ=255)
                                                                                                                                   ccAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGcc                                                                     >  8:54802/1‑94 (MQ=255)
                                                                                                                                            ggTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGAACAACGACCGCGCAGCCTCCAGCCATTCCATTTCGGCCATTTGCAGTGGCAGTTGa     <  8:136776/149‑1 (MQ=255)
                                                                                                                                               ccACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGAACAACGACCGCGCAGCCTCCAGCCATTCCATTTCGGCCATTTGCAGTGGCAGTTGATgg  >  6:288248/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CTGTTGCTGCCAGCGCGACAACAACGTCTCTGCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGAACAACGACCGCGCAGCCTCCAGCCATTCCATTTCGGCCATTTGCAGTGGCAGTTGATGG  >  NC_002947/5684667‑5684958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑