Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,741,209	A→G	5.5%	L41L (TTG→CTG)	PP_5038 ←	lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,741,209	0	A	G	5.5%	220.5 / 2.4	73	L41L (TTG→CTG)	PP_5038	lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (31/38);  new base G (2/2);  total (33/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

CGCCTCATTTGAGGCCCTGCCCGGAATACATCAGCACCTTGTCAGCTGCGTACTGCACGCTGATGAAGCTCTTCTCGTCCCCCCAGGTGCAGCTGCTCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTGCTGCAGGCAGTCAGCACGACGCACAGCGACAACAGGGCGAGGGAACGCAACGACATGAAGGGACACTCCTGAAAGATGATACAG  >  NC_002947/5741071‑5741357                                                                                                                                           |                                                                                                                                                     cgcCTCATTTGAGGCCCTGCCCGGAATACATCAGCACCTTGTCAGCTGCGTACTGCACGCTGATGAAGCTCTTCTCGTCCCCCCAGGTGCAGCTGCTCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGAcc                                                                                                                                            <  2:333151/149‑1 (MQ=255) cgcCTCATTTGAGGCCCTGCCCGGAATACATCAGCACCTTGTCAGCTGCGTACTGCACGCTGATGAAGCTCTTCTCGTCCCCCCAGGTGCAGCTGCTCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGAcc                                                                                                                                            <  3:287339/149‑1 (MQ=255)  gcCTCATTTGAGGCCCTGCCCGGAATACATCAGCACCTTGTCAGCTGCGTACTGCACGCTGATGAAGCTCTTCTCGTCCCCCCAGGTGCAGCTGCTCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAGTTGCTCggcac                                                                                                                                           <  3:279254/149‑5 (MQ=255)    cTCATTTGAGGCCCTGCCCGGAATACATCAGCACCTTGTCAGCTGCGTACTGCACGCTGATGAAGCTCTTCTCGTCCCCCCAGGTGCAGCTGCTCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCg                                                                                                                                         >  5:268394/1‑149 (MQ=255)            aGGCCCTGCCCGGAATACATCAGCACCTTGTCAGCTGCGTACTGCACGCTGATGAAGCTCTTCTCGTCCCCCCAGGTGCAGCTGCTCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGtt                                                                                                                                 >  2:274228/1‑149 (MQ=255)            aGGCCCTGCCCGGAATACATCAGCACCTTGTCAGCTGCGTACTGCACGCTGATGAAGCTCTTCTCGTCCCCCCAGGTGCAGCTGCTCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGt                                                                                                                                  <  6:41751/148‑1 (MQ=255)             ggCCCTGCCCGGAATACATCAGCACCTTGTCAGCTGCGTACTGCACGCTGATGAAGCTCTTCTCGTCCCCCCAGGTGCAGCTGCTCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTc                                                                                                                                <  3:203330/149‑1 (MQ=255)             ggCCCTGCCCGGAATACATCAGCACCTTGTCAGCTGCGTACTGCACGCTGATGAAGCTCTTCTCGTCCCCCCAGGTGCAGCTGCTCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTc                                                                                                                                <  6:268394/149‑1 (MQ=255)                    cccGGAATACATCAGCACCTTGTCAGCTGCGTACTGCACGCTGATGAAGCTCTTCTCGTCCCCCCAGGTGCAGCTGCTCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCgg                                                                                                                         >  7:82304/1‑149 (MQ=255)                       ggAATACATCAGCACCTTGTCAGCTGCGTACTGCACGCTGATGAAGCTCTTCTCGTCCCCCCAGGTGCAGCTGCTCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCt                                                                                                                      <  1:274228/149‑1 (MQ=255)                           tACATCAGCACCTTGTCAGCTGCGTACTGCACGCTGATGAAGCTCTTCTCGTCCCCCCAGGTGCAGCTGCTCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGAt                                                                                                                  <  3:348952/149‑1 (MQ=255)                              aTCAGCACCTTGTCAGCTGCGTACTGCACGCTGATGAAGCTCTTCTCGTCCCCCCAGGTGCAGCTGCTCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCtt                                                                                                               >  4:293465/1‑149 (MQ=255)                               tCAGCACCTTGTCAGCTGCGTACTGCACGCTGATGAAGCTCTTCTCGTCCCCCCAGGTGCAGCTGCTCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAAGTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCt                                                                                                                >  6:196951/1‑147 (MQ=255)                               tCAGCACCTTGTCAGCTGCGTACTGCACGCTGATGAAGCTCTTCTCGTCCCCCCAGGTGCAGCTGCTCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTg                                                                                                              >  4:348783/1‑149 (MQ=255)                               tCAGCACCTTGTCAGCTGCGTACTGCACGCTGATGAAGCTCTTCGCTTCCCCCCAGGTGCAGCTGCTCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTg                                                                                                              <  1:263048/149‑1 (MQ=255)                               tCAGCACCTTGTCACCTGCGTACTGCACGCTGATGAAGCTCTTCTCGTCCCCCCAGGGGCAGCTGCTCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTg                                                                                                              <  4:338481/149‑1 (MQ=255)                                      cTTGTCAGCTGCGTACTGCACGCTGATGAAGCTCTTCTCGTCCCCCCAGGTGCAGCTGCTCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGt                                                                                                       <  5:59187/149‑1 (MQ=255)                                         gTCAGCTGCGTACTGCACGCTGATGAAGCTCTTCTCGTCCCCCCAGGTGCAGCTGCTCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGttt                                                                                                     <  7:113400/148‑1 (MQ=255)                                            aGCTGCGTACTGCACGCTGATGAAGCTCTTCTCGTCCCCCCAGGTGCAGCTGCTCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTcc                                                                                                  <  1:380239/148‑1 (MQ=255)                                             gCTGCGTACTGCACGCTGATGAAGCTCTTCTCGTCCCCCCAGGTGCAGCTGCTCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGc                                                                                                                                                  <  2:12555/99‑1 (MQ=255)                                             gCTGCGTACTGCACGCTGATGAAGCTCTTCTCGTCCCCCCAGGTGCAGCTGCTCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGc                                                                                                                                                  >  1:12555/1‑99 (MQ=255)                                                 cGTACTGCACGCTGATGAAGCTCTTCTCGTCCCCCCAGGTGCAGCTGCTCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCtgat                                                                                              <  2:34647/147‑1 (MQ=255)                                                 cGTACTGCACGCTGATGAAGCTCTTCTCGTCCCCCCAGGTGCAGCTGCTCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCtgat                                                                                              <  5:240165/147‑1 (MQ=255)                                                     cTGCACGCTGATGAAGCTCTTCTCGTCCCCCCAGGTGCAGCTGCTCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATc                                                                                         <  1:11957/148‑1 (MQ=255)                                                      tGCACGCTGATGAAGCTCTTCTCGTCCCCCCAGGTGCAGCTGCTCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCtt                                                                                       >  8:23209/1‑149 (MQ=255)                                                      tGCACGCTGATGAAGCTCTTCTCGTCCCCCCAGGTGCAGCTGCTCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCtt                                                                                       >  2:232684/1‑149 (MQ=255)                                                         aCGCTGATGAAGCTCTTCTCGTCCCCCCAGGTGCAGCTGCTCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTtgct                                                                                    <  8:82304/149‑1 (MQ=255)                                                              gatgaAGCTCTTCTCGTCCCCCCAGGTGCAGCTGCTCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTGCTgcagg                                                                               >  1:335107/1‑149 (MQ=255)                                                                  aaGCTCTTCTCGTCCCCCCAGGTGCAGCTGCTCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTGCTgcaggcag                                                                            >  2:180900/1‑148 (MQ=255)                                                                     ctctTCTCGTCCCCCCAGGTGCAGCTGCTCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTGCTGCAGGCAGTCAg                                                                        <  3:348783/149‑1 (MQ=255)                                                                       cttctCGTCCCCCCAGGTGCAGCTGCTCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTGCTGCAGGCAGTCAGCa                                                                      >  1:18197/1‑149 (MQ=255)                                                                        ttctCGTCCCCCCAGGTGCAGCTGCTCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTGCTGATTGAACTTGCTGCAGGCAGTCAGCac                                                                     >  8:344433/1‑149 (MQ=255)                                                                        ttctCGTCCCCCCAGGTGCAGCTGCTCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTGCTGCAGGCAGTCAGCac                                                                     >  3:362956/1‑149 (MQ=255)                                                                        ttctCGTCCCCCCAGGTGCAGCTGCTCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTGCTGCAGGCAGTCAGCac                                                                     >  3:49027/1‑149 (MQ=255)                                                                             gTCCCCCCAGGTGCAGCTGCTCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTGCTGCAGGCAGTCAGCacgacg                                                                 >  1:10844/1‑148 (MQ=255)                                                                                cccccAGGTGCAGCTGCTCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTGCTGCAGGCAGTCAGCACGACGca                                                               >  4:368081/1‑147 (MQ=255)                                                                                 ccccAGGTGCAGCTGCGCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTGCTGCAGGCAGTCAGCACGACGCACAg                                                            <  6:231848/149‑1 (MQ=255)                                                                                  cccAGGTGCAGCTGCTCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTGCTGCAGGCAGTCAGCAGGACGCACAGc                                                           >  7:207088/1‑149 (MQ=255)                                                                                              tgctCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTGCTGCAGGCAGTCAGCACGACGCACAGCGACAACAGGGc                                                >  6:304774/1‑148 (MQ=255)                                                                                                 tCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCtga                                                                                               <  4:131524/98‑1 (MQ=255)                                                                                                 tCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCtga                                                                                               >  3:131524/1‑98 (MQ=255)                                                                                                 tCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTGCTGCAGGCAGTCAGCa                                                                      <  6:383574/123‑1 (MQ=255)                                                                                                 tCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTGCTGCAGGCAGTCAGCa                                                                      >  5:383574/1‑123 (MQ=255)                                                                                                 tCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTGCTGCAGGCAGTCAGCACGACGCACAGCGacaaca                                                    <  6:99161/141‑1 (MQ=255)                                                                                                 tCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTGCTGCAGGCAGTCAGCACGACGCACAGCGacaaca                                                    >  5:99161/1‑141 (MQ=255)                                                                                                 tCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTGCTGCAGGCAGTCAGCACGACGCACAGCGACAACAGGGCGAgg                                            >  8:288499/1‑149 (MQ=255)                                                                                                 tCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTGCTGCAGGCAGTCAGCACGACGCACAGCGACAACAGGGCGAgg                                            >  7:49732/1‑149 (MQ=255)                                                                                                   aTGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTGCTGCAGGCAGTCAGCACGACGCACAGCGACAACAGGGCGAggg                                           >  2:306234/1‑148 (MQ=255)                                                                                                   aTGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTGCTGCAGGCAGTCAGCACGACGCACAGCGACAACAGGGCGAggg                                           >  2:310889/1‑148 (MQ=255)                                                                                                   aTGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTGCTGCAGGCAGTCAGCACGACGCACAGCGACAACAGGGCGAgg                                            <  2:18197/147‑1 (MQ=255)                                                                                                   aTGCCCAACGCGCCAGAACACTCGCTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCCGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTGCTGCAGGCAGTCAGCACGACGCACAGCGACAACAGGGCGAgg                                            <  6:121108/147‑1 (MQ=255)                                                                                                   aTGCCCAACGCGCAAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCTGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTGCTGCAGTCAGTCAGCACTACGAACAGCGACAACAGGGAGAgg                                            <  3:223560/147‑1 (MQ=255)                                                                                                    tGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGAAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTCCTGCAGGCAGTCAGCACGACGCACAGCGACAACAGGGCGAGGCaa                                         >  5:372523/1‑149 (MQ=255)                                                                                                       ccAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTGCTGCAGGCAGTCAGCAGGACGCACAGCGACAACAGGGCGAGGGAACGc                                      <  8:207088/149‑1 (MQ=255)                                                                                                       ccAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTGCTGCAGGCAGTCAGCACGACGCACAGCGACAACAGGGCGAGGGAACg                                       >  3:50668/1‑148 (MQ=255)                                                                                                         aaCGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTGCTGCAGGCAGTCAGCACGACGCACAGCGACAACAGGGCGAGGGAACGCaa                                    >  7:272482/1‑149 (MQ=255)                                                                                                          aCGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGTCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTGCTGCAGGCAGTCAGCGCGACGCACAGCGACAACAGGGCGAGGGAACGCAAc                                   <  8:276756/149‑1 (MQ=255)                                                                                                          aCGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTGCTGCAGGCAGTCAGCACGACGCACAGCGACAACAGGGCGAGGGAACGCAAc                                   <  2:335107/149‑1 (MQ=255)                                                                                                          aCGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTGCTGCAGGCAGTCAGCACGACGCACAGCGACAACAGGGCGAGGGAACGCAAc                                   <  1:232684/149‑1 (MQ=255)                                                                                                             cgcCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTGCTGCAGGCAGTCAGCACGACGCACAGCGACAACAGGGCGAGGGAACGCAACGAc                                <  8:49732/149‑1 (MQ=255)                                                                                                             cgcCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTGCTGCAGGCAGTCAGCACGACGCACAGCGACAACAGGGCGAGGGAACGCAACGAc                                <  4:362956/149‑1 (MQ=255)                                                                                                                   aaCAGTCGGTCGGCGTGCCGAGCAACTGCTCGAACTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTGCTGCAGGCAGTCAGCACGACGCACAGCGACAACAGGGCGAGGGAACGCAACGACATGAAg                          <  6:372523/149‑1 (MQ=255)                                                                                                                     cacTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTGCTGCAGGCAGTCAGCACGACGCACAGCGACAACAGGGCGAGGGAACGCAACGACATGAAggg                        <  4:50668/149‑1 (MQ=255)                                                                                                                      acTCGGTCGGCGTGCCGAGCAACTGCTCGACCACGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTGCTGCAGGCAGTCAGCACGACGCACAGCGACAACAGGGCGAGGGAACGCAACGACATGAAGGGa                       >  2:107936/1‑149 (MQ=255)                                                                                                                          ggtcggCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTGCTGCAGGCAGTCAGCACGACGCACAGCGACAACAGGGCGAGGGAACGCAACGa                                 <  3:220128/135‑1 (MQ=255)                                                                                                                          ggtcggCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTGCTGCAGGCAGTCAGCACGACGCACAGCGACAACAGGGCGAGGGAACGCAACGa                                 >  4:220128/1‑135 (MQ=255)                                                                                                                                 gTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTGCTGCAGGCAGTCAGCACGACGCACAGCGACAACAGGGCGAGGGAACGCAACGACATGAAGGGACACTCCTGaaa            <  3:293465/149‑1 (MQ=255)                                                                                                                                 aggtcGAGCAGTTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTGCTGCAGGCAGTCAGCACGACGCACAGCGACAACAGGGCGAGGGAACGCAACGACATGAAGGGACACTCCTGa              >  8:289589/5‑147 (MQ=255)                                                                                                                                   gtcGAGCAGTTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTGCTGCAGGCAGTCAGCACGACGCACAGCGACAACAGGGCGAGGGAACGCAACGACATGAAGGGACACTCCTGa              >  2:287997/3‑145 (MQ=255)                                                                                                                                    tcGAGCAGTTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTGCTGCAGGCAGTCAGCACGACGCACAGCGACAACAGGGCGAGGGAACGCAACGACATGAAGGGACACTCCTGAAAgat         <  7:289589/148‑1 (MQ=255)                                                                                                                                     cGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTGCTGCAGGCAGTCAGCACGACGCACAGCGACAACAGGGCGAGGGAACGCAACGACATGAAGGGACACTCCTGAAAgatg        <  2:10844/149‑1 (MQ=255)                                                                                                                                      gAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTGCTGCAGGCAGTCAGCACGACGCACAGCGACAACAGGGCGAGGGAACGCAACGACATGAAGGGACACTCCTGAAAgatga       <  3:368081/149‑1 (MQ=255)                                                                                                                                      gAGCAACTGCTCGACCACGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTGCTGCAGGCAGTCAGCACGACGCACAGCGACAACAGGGCGAGGGAACGCAACGACATGAAGGGACACTCCTGAAAgatga       <  1:107936/149‑1 (MQ=255)                                                                                                                                           aCTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTGCTGCAGGCAGTCAGCACGACGCACAGCGACAACAGGGCGAGGGAACGCAACGACATGAAGGGACACTCCTGAAAGATGATACAg  >  4:220984/1‑149 (MQ=255)                                                                                                                                           |                                                                                                                                                     CGCCTCATTTGAGGCCCTGCCCGGAATACATCAGCACCTTGTCAGCTGCGTACTGCACGCTGATGAAGCTCTTCTCGTCCCCCCAGGTGCAGCTGCTCATGCCCAACGCGCCAGAACACTCGGTCGGCGTGCCGAGCAACTGCTCGACCTCGGCCTTGTTCATACCGGCCTTGATCTTGGAATAGTTTTCCTGATTGATCTTGCTGCAGGCAGTCAGCACGACGCACAGCGACAACAGGGCGAGGGAACGCAACGACATGAAGGGACACTCCTGAAAGATGATACAG  >  NC_002947/5741071‑5741357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑