Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,750,060	C→T	7.1%	intergenic (‑81/+48)	typA ← / ← thiI	ribosome associated GTPase/tRNA sulfurtransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,750,060	0	C	T	7.1%	163.2 / 2.2	56	intergenic (‑81/+48)	typA/thiI	ribosome associated GTPase/tRNA sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base C (27/25);  new base T (2/2);  total (29/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.84e-01

TCGACCAGAGTGGTTTTACCATGGTCAACGTGGGCGATGATGGCGATGTTACGCAGATTTTCGATCACAAGTGTATCTCGATCAGAGGATTCGGTTGCCGCCCAGTGTAGGCGGCCAATATGTACCGTTTAAGGCTCAGCGGGCCCGGCGGTCGGGAGGGCGATGGCGAATACTGCCGCCATACAGCCCCGGCGTCTTATGTCGGACGATAAA‑CACGCACATTGGCATGTCCCTCACTGAGCAGATGGTGTGCGTGCAAACGGCTCATCACACCCTTGTCGCAATACAGCAAGT  >  NC_002947/5749912‑5750205                                                                                                                                                     |                                                                                                                                                   tCGACCAGAGTGGTTTTACCATGGTCAACGTGGGCGATGATGGCGATGTTACGCAGATTTTCGATCACAAGTGTATCTCGATCAGAGGATTCGGTTGCCGCCCAGTGTAGGCGGCCAATATGTACCGTTTAAGGCTCAGCGGGCCcggc                                                                                                                                                    >  5:332231/1‑149 (MQ=255)   gACCAGAGTGGTTTTACCATGGTCAACGTGGGCGATGATGGCGATGTTACGCAGATTTTCGATCACAAGTGTATCTCGATCAGAGGATTCGGTTGCCGCCCAGTGTAGGCGGCCAATATGTACCGTTTAAGGCTCAGCGGGCCcggcgg                                                                                                                                                  >  2:161807/1‑149 (MQ=255)   gACCAGAGTGGTTTTACCATGGTCAACGTGGGCGATGATGGCGATGTTACGCAGATTTTCGATCACAAGTGTATCTCGATCAGAGGATTCGGTTGCCGCCCAGTGTAGGCGGCCAATATGTACCGTTTAAGGCTCAGCGGGCCcggcgg                                                                                                                                                  >  3:118833/1‑149 (MQ=255)    aCCAGAGTGGTTTTACCATGGTCAACGTGGGCGATGATGGCGATGTTACGCAGATTTTCGATCACAAGTGTATCTCGATAAGAGGATTCGGTTGCCGCCCAGTGTAGGCGGCCAATATGTACCGTTTAAGGCTCAGCGGGCCCGGCGGt                                                                                                                                                 >  6:235961/1‑149 (MQ=255)      cAGAGTGGTTTTACCATGGTCAACGTGGGCGATGATGGCGATGTTACGCAGATTTTCGATCACAAGTGTATCTCGATCAGAGGATTCGGTTGCCGCCCAGTGTAGGCGGCCAATATGTACCGTTTAAGGCTCAGCGGGCCCGGCGGTCg                                                                                                                                               <  8:340539/149‑1 (MQ=255)             gTTTTACCATGGTCAACGTGGGCGATGATGGCGATGTTACGCAGATTTTCGATCACAAGTGTATCTCGATCAGAGGATTCGGTTGCCGCCCAGTGTAGGCGGCCAATATGTACCGTTTAAGGCTCAGCGGGCCCGGCGGTCGGGAGGGc                                                                                                                                        >  2:136705/1‑149 (MQ=255)                  aCCATGGTCAACGTGGGCGATGATGGCGATGTTACGCAGATTTTCGATCACAAGTGTATCTCGATCAGAGGATTCGGTTGCCGCCCAGTGTAGGCGGCCAATATGTACCGTTTAAGGCTCAGCGGGCCCGGCGGTCGGGAGGGCGATgg                                                                                                                                   >  8:245077/1‑149 (MQ=255)                   ccATGGTCAACGTGGGCGATGATGGCGATGTTACGCAGATTTTCGATCACAAGTGTATCTCGATCAGAGGATTCGGTTGCCGCCCAGTGTAGGCGGCCAATATGTACCGTTTAAGGCTCAGCGGGCCCGGCGGTCGGGAGGGCGATgg                                                                                                                                   <  5:260053/148‑1 (MQ=255)                      tGGTCAACGTGGGCGATGATGGCGATGTTACGCAGATTTTCGATCACAAGTGTATCTCGATCAGAGGATTCGGTTGCCGCCCAGTGTAGGCGGCCAATATGTACCGTTTAAGGCTCAGCGGGCCCGGCGGTCGGGAGGGCGATGGCGaa                                                                                                                               <  5:67171/149‑1 (MQ=255)                        gTCAACGTGGGCGATGATGGCGATGTTACGCAGATTTTCGATCACAAGTGTATCTCGATCAGAGGATTCGGTTGCCGCCCAGTGTAGGCGGCCAATATGTACCGTTTAAGGCTCAGCGGGCCCGGCGGTCGGGAGGGCGATGGCGAATa                                                                                                                             <  3:78521/149‑1 (MQ=255)                        gTCAACGTGGGCGATGATGGCGATGTTACGCAGATTTTCGATCACAAGTGTATCTCGATCAGAGGATTCGGTTGCCGCCCAGTGTAGGCGGCCAATATGTACCGTTTAAGGCTCAGCGGGCCCGGCGGTCGGGAGGGCGATGGCGAATa                                                                                                                             <  4:118833/149‑1 (MQ=255)                        gTCAACGTGGGCGATGATGGCGAAGTTACGCAGATTTTCGATCACAAGTGTATCTCGATCAGAGGATTCGGTTGCCGCCCAGTGTAGGCGGCCAATATGTACCGTTTAAGGCTCAGCGGGCCCGGCGGTCGGGAGGGCGATGGCGAATa                                                                                                                             <  8:311253/149‑1 (MQ=255)                         tCAACGTGGGCGATGATGGCGATGTTACGCAGATTTTCGATCACAAGTGTATCTCGATCAGAGGATTCGGTTGCCGCCCAGTGTAGGCGGCCAATATGTACCGTTTAAGGCTCAGCGGGCCCGGCGGTCGGGAGGGCGATGGCGAATAc                                                                                                                            >  7:345908/1‑149 (MQ=255)                                  gCGATGATGGCGATGTTACGCAGATTTTCGATCACAAGTGTATCTCGATCAGAGGATTCGGTTGCCGCCCAGTGTAGGCGGCCAATATGTACCGTTTAAGGCTCAGCGGGCCCGGCGGTCGGGAGGGCGATGGCGAATACTGCCGCCAt                                                                                                                   <  7:245077/149‑1 (MQ=255)                                   cGATGATGGCGATGTTACGCAGATTTTCGATCACAAGTGTATCTCGATCAGAGGATTCGGTTGCCGCCCAGTGTAGGCGGCCAATATGTACCGTTTAAGTCTCAGCGGGCCCGGCGGTCGGGAGGGCGATGGCGAATACTGCAGCCAt                                                                                                                   >  5:215727/1‑148 (MQ=255)                                            cGATGTTACGCAGATTTTCGATCACAAGTGTATCTCGATCAGAGGATTCGGTTGCCGCCCAGTGTAGGCGGCCAATATGTACCGTTTAAGGCTCAGCGGGCCCGGCGGTCGGGAGGGCGATGGCGAATACTGCCGCCATACAGCCCCgg                                                                                                         <  8:345908/149‑1 (MQ=255)                                            cGATGTTACGCAGATTTTCGATCACAAGTGTATCTCGATCAGAGGATTCGGTTGCCGCCCAGTGTAGGCGGCCAATATGTACCCTTTAAGGCTCAGCGGGCCCGGCGGTCGGGAGGGCGATGGCGAATACTGCCGCCATACAGCCCCgg                                                                                                         >  7:208358/1‑149 (MQ=255)                                                     gCAGATTTTCGATCACAAGTGTATCTCGATCAGAGGATTCGGTTGCCGCCCAGTGTAGGCGGCCAATATGTACCGTTTAAGGCTCAGCGGGCCCGGCGGTCGGGAggg                                                                                                                                         >  8:184763/1‑108 (MQ=255)                                                     gCAGATTTTCGATCACAAGTGTATCTCGATCAGAGGATTCGGTTGCCGCCCAGTGTAGGCGGCCAATATGTACCGTTTAAGGCTCAGCGGGCCCGGCGGTCGGGAggg                                                                                                                                         <  7:184763/108‑1 (MQ=255)                                                     gCAGATTTTCGATCACAAGTGTATCTCGATCAGAGGATTCGGTTGCCGCCCAGTGTAGGCGGCCAATATGTACCGTTTAAGGCTCAGCGGGCCCGGCGGTCGGGAGGGCGATGGCGAATACTGCCGCCATACAGCCCCGGCGTCTTATg                                                                                                >  1:91167/1‑149 (MQ=255)                                                     gCAGATTTTCGATCACAAGTGTATCTCGATCAGAGGATTCGGTTGCCGCCCAGTGCAGGCGGCCAATATGTACCGTTTAAGGCTCAGCGGGCCCGGCGGTCGGGAGGGCGATGGCGaa                                                                                                                               >  8:214086/1‑118 (MQ=255)                                                         aTTTTCGATCACAAGTGTATCTCGATCAGAGGATTCGGTTGCCGCCCAGTGTAGGCGGCCAATATGTACCGTTTAAGGCTCAGCGGGCCCGGCGGTCGGGAGGGCGATGGCGAATACTGCCGCCATACAGCCCCGGc                                                                                                        <  6:63790/137‑1 (MQ=255)                                                         aTTTTCGATCACAAGTGTATCTCGATCAGAGGATTCGGTTGCCGCCCAGTGTAGGCGGCCAATATGTACCGTTTAAGGCTCAGCGGGCCCGGCGGTCGGGAGGGCGATGGCGAATACTGCCGCCATACAGCCCCGGc                                                                                                        >  5:63790/1‑137 (MQ=255)                                                               gATCACAAGTGTATCTCGATCAGAGGATTCGGTTGCCGCCCAGTGTAGGCGGCCAATATGTACCGTTTAAGGCTCAGCGGGCCCGGCGGTCGGGAGGGCGATGGCGAATACTGCCGCCATACAGCCCCGGCGTCTTATGTCGGACGATa                                                                                      <  6:110545/149‑1 (MQ=255)                                                                             ctcGATCAGAGGATTCGGTTGCCGCCCAGTGTAGGCGGCCAATATGTACCGTTTAAGGCTCCGCGGGCCCGGCGGTCGGGAGGGCGATGGCGATTACTGCCGCCATACAGCCCCGGCGTCTTATGTCGGACGATAAA‑CACGCACATTgg                                                                       >  6:292362/1‑149 (MQ=255)                                                                               cGATCAGAGGATTCGGTTGCCGCCCAGTGTAGGCGGCCAATATGTACCGTTTAAGGCTCAGCGGGCCCGGCGGTCGGGAGGGCGATGGCGAATACTGCCGCCATACAGCCCCGGCGTCTTATGTCGGACGATAAA‑CACGCACATTGGc                                                                      <  1:136705/148‑1 (MQ=255)                                                                                gATCAGAGGTTTCGGGTGCCGGCCAGTGTATGCGGCCAATATGTACCGTTTAAGGCTCAGCGGGCCCGGCGGTCGGGAGGGCGATTGCGAATACTGCCGCCATACATCCCCGGCGTCTTATTTCGGAAGATAAA‑CACGCACATTg                                                                        <  6:215727/145‑1 (MQ=255)                                                                                gATCAGAGGATTCGGTTGCCGCCCAGTGTAGGCGGCCAATATGTACCGTTTAAGGCTCAGCGGGCCCGGCGGTCGGGAGGGCGATGGCGAATACTGCCGCCATACAGCCCCGGCGTCTTATGTCGGACGATAAA‑CACGCACATTGGCAt                                                                    >  5:134797/1‑149 (MQ=255)                                                                                    agagGATTCGGTTGCCGCCCAGTGTAGGCGGCCAATATGTACCGTTTAAGGCTCAGCGGGCCCGGCGGTCGGGAGGGCGATGGCGAATACTGCCGCCATACAGCCCCGGCGTCTTATGTCGGACGATAAA‑CACGCACATTGGCATGTcc                                                                >  7:353701/1‑149 (MQ=255)                                                                                     gagGATTCGGTTGCCGCCCAGTGTAGGCGGCCAATATGTACCGTTTAAGGCTCAGCGGGCCCGGCGGTCGGGAGGGCGATGGCGAATACTGCCGCCATACAGCCCCGGCGTCTTATGTCGGACGATAAA‑CACGCACATTGGCATGTccc                                                               >  2:288392/1‑149 (MQ=255)                                                                                        gATTCGGTTGCCGCCCAGTGTAGGCGGCCAATATGTACCGTTTAAGGCTCAGCGGGCCCGGCGGTCGGGAGGGCGATGGCGAATACTGCCGCCATACAGCCCCGGCGTCTTATGTCGGACGATAAA‑CACGCACATTGGCATGTCCCTCa                                                            >  1:195744/1‑149 (MQ=255)                                                                                         aTTCGGTTGCCGCCCAGTGTAGGCGGCCAATATGTACCGTTTAAGGCTCAGCGGGCCCGGCGGTCGGGAGGGCGATGGCGAATACTGCCGCCATACAGCCCCGGCGTCTTATGTCGGACGATAAA‑CACGCACATTGGCATGTCCCTCAc                                                           >  8:325691/1‑149 (MQ=255)                                                                                          ttCGGTTGCCGCCCAGTGTAGGCGGCCAATATGTACCGTTTAAGGCTCAGCGGGCCCGGCGGTCGGGAGGGCGATGGCGAATACTGCCGCCATACAGCCCCGGCGTCTTATGTCGGACGATAAA‑CACGCACATTGGCATGTCCCTCACt                                                          >  1:9640/1‑149 (MQ=255)                                                                                             ggTTGCCGCCCAGTGTAGGCGGCCAATATGTACCGTTTAAGGCTCAGCGGGCCCGGCGGTCGGGAGGGCGATGGCGAATACTGCCGCCATACAGCCCCGGCGTCTTATGTCGGACGATAAA‑CACGCACATTGGCATGTCCCTCACTGa                                                        <  8:208358/148‑1 (MQ=255)                                                                                              gTTGCCGCCCAGTGTAGGCGGCCAATATGTACCGTTTAAGGCTCAGCGGGCCCGGCGGTCGGGAGGGCGATGGCGAATACTGCCGCCATACAGCCCCTGCGTCTTATGTCGGACGATAAA‑CACGCACATTGGCATGTCCCTCACTGAGc                                                      <  5:235961/149‑1 (MQ=255)                                                                                                  gctcccAGTGTATGCGGCCAATAGGTACCGTTTAATGCTCAGCGGGCCCGGCGTTCGGGAGGGCGATTGCTAATACTGCAGCCATACATCCCCGGCGTCTTATGTCGGACGATAAA‑CACGCACATTGGCATGTACCTCACTGAGCAGAt                                                  <  8:110425/146‑1 (MQ=255)                                                                                                   cgccCAGTGTAGGCGGCCAATATGTACCGTTTAAGGCTCAGCGGGCCCGGCGGTCGGGAGGGCGATGGCGAATACTGCCGCCATACAGCCCCGGCGTCTTATGTCGGACGATAAA‑CACGCACATTGGCATGTCCCTCACTGAGCAGATg                                                 <  7:100358/149‑1 (MQ=255)                                                                                                       cAGTGTAGGCGGCCAATATGTACCGTTTAACGCTCAGCGGGCCCGGCGGTCGGGAGGGCGATGGCGAATACTGCCGCCATACAGCCCCGGCGTCTTATGTCGGACGATAAA‑CACGCACATTGGCATGTCCCTCACTGAGCAGATGgtgt                                             <  4:338466/149‑1 (MQ=255)                                                                                                              ggcggcCAATATGTACCGTTTAAGGCTCAGCGGGCCCGGCGGTCGGGAGGGCGATGGCGAATACTGCCGCCATACAGCCCCGGCGTCTTATGTCGGACGATAAA‑CACGCACATTGGCATGTCCCTCACTGAGCAGATGGTgtgcgtgc                                       <  1:161807/148‑1 (MQ=255)                                                                                                                  gcCAATATGTACCGTTTAAGGCTCAGCGGGCCCGGCGGTCGGGAGGGCGATGGCGAATACTGCCGCCATACAGCCCCGGCGTCTTATGTCGGACGATAAA‑CACGCACATTGGCATGTCCCTCACTGAGCAGATGGTGTGCGTGCAAACg                                  >  7:94102/1‑149 (MQ=255)                                                                                                                     aaTATGTACCGTTTAAGGCTCAGCGGGCCCGGCGGTCGGGAGGGCGATGGCGAATACTGCCGCCATACAGCCCCGGCGTCTTATGTCGGACGATAAA‑CACGCACATTGGCATGTCCCTCACTGAGCAGATGGTGTGCGTGCAAACGGCt                               >  4:299268/1‑149 (MQ=255)                                                                                                                         tGTACCGTTTAAGGCTCAGCGGGCCCGGCGGTCGGGAGGGCGATGGCGAATACTGCCGCCATACAGCCCCGGCGTCTTATGTCGGACGATAAACCACGCACATTGGCATGTCCCTCACTGAGCAGATGGTGTGCGTGCAAACGGCtca                             <  1:288392/148‑1 (MQ=255)                                                                                                                         tGTACCGTTTAAGGCTCAGCGGGCCCGGCGGTCGGGAGGGCGATGGCGAATACTGCCGCCATACAGCCCCGGCGTCTTATGTCGGACGATAAA‑CACGCACATTGGCATGTCCCTCACTGAGCAGATGGTGTGCGTGCAAACGGCtcatc                           <  8:94102/149‑1 (MQ=255)                                                                                                                           tACCGTTTAAGGCTCAGCGGGCCCGGCGGTCGGGAGGGCGATGGCGAATACTGCCGCCATACAGCCCCGGCGTCTTATGTCGGACGATAAA‑CACGCACATTGGCATGTCCCTCACTGAGCAGATGGTGTGCGTGCAAACGGCtcatca                          >  6:115030/1‑148 (MQ=255)                                                                                                                           tACCGTTTAAGGCTCAGCGGGCCCGGCGGTCGGGAGGGCGATGGCGAATACTGCCGCCATACAGCCCCGGCGTCTTATGTCGGACGATAAA‑CACGCACATTGGCATGTCCCTCACTGAGCAGATGGTGTGCGTGCAAACGGCtcatca                          >  8:29868/1‑148 (MQ=255)                                                                                                                             acGTTTAAGGCTCAGCGGGCCCCGCGGTCGGGAGGGCGAGGGCGAATACTGCCGCCATACAGCCCCGGCGTCTTATGTCGGACGATAAA‑CACGCACATTGGCATGTCCCTCACTGAGCAGATGGTGTGCGTGCAAACGGCTCATcaca                        <  6:231144/147‑1 (MQ=255)                                                                                                                              cGTTTAAGGCTCAGCGGGCCCGGTGGTCGGGAGGGCGATGGCGAATACTGCCGCCATACAGCCCCGGCGTCTTATGTCGGACGATAAA‑CACGCACATTGGCATGTCCCTCACTGAGCAGATGgtgt                                             <  5:177814/126‑1 (MQ=255)                                                                                                                              cGTTTAAGGCTCAGCGGGCCCGGTGGTCGGGAGGGCGATGGCGAATACTGCCGCCATACAGCCCCGGCGTCTTATGTCGGACGATAAA‑CACGCACATTGGCATGTCCCTCACTGAGCAGATGgtgt                                             >  6:65551/1‑126 (MQ=255)                                                                                                                              cGTTTAAGGCTCAGCGGGCCCGGTGGTCGGGAGGGCGATGGCGAATACTGCCGCCATACAGCCCCGGCGTCTTATGTCGGACGATAAA‑CACGCACATTGGCATGTCCCTCACTGAGCAGATGgtgt                                             >  6:177814/1‑126 (MQ=255)                                                                                                                              cGTTTAAGGCTCAGCGGGCCCGGTGGTCGGGAGGGCGATGGCGAATACTGCCGCCATACAGCCCCGGCGTCTTATGTCGGACGATAAA‑CACGCACATTGGCATGTCCCTCACTGAGCAGATGgtgt                                             <  5:65551/126‑1 (MQ=255)                                                                                                                                       cTCAGCGGGCCCGGCGGTCGGGAGGGCGATGGCGAATACTGCCGCCATACAGCCCCGGCGTCTTATGTCGGACGATAAA‑CACGCACAATGGCATGTCCCTCACTGAGCAGATGGTGTGCGTGCAAACGGCTCATCACACCCTTGTCGc              >  5:228357/1‑148 (MQ=255)                                                                                                                                        tCAGCGGGCCCGGCGGTCGGGAGGGCGATGGCGAATACTGCCGCCATACAGCCCCGGCGTCTTATGTCgg                                                                                            >  7:160895/1‑70 (MQ=255)                                                                                                                                        tCAGCGGGCCCGGCGGTCGGGAGGGCGATGGCGAATACTGCCGCCATACAGCCCCGGCGTCTTATGTCgg                                                                                            <  8:160895/70‑1 (MQ=255)                                                                                                                                        tCAGCGGGCCCGGCGGTCGGGAGGGCGATGGCGAATACTGCCGCCATACAGCCCCGGCGTCTTATGTCGGACGATAAA‑CACGCACATTGGCATGTCCCt                                                              >  6:34224/1‑99 (MQ=255)                                                                                                                                        tCAGCGGGCCCGGCGGTCGGGAGGGCGATGGCGAATACTGCCGCCATACAGCCCCGGCGTCTTATGTCGGACGATAAA‑CACGCACATTGGCATGTCCCt                                                              <  5:34224/99‑1 (MQ=255)                                                                                                                                          aGCGGGCCCGGCGGTCGGGAGGGCGATGGCGAATACTGCCGCCATACAGCCCCGGCGTCTTATGTCGGACGATAAA‑CACGCACATTGGCATGTCCCTCACTGAGCAGATGGTGTGCGTGCAAACGGCTCATCACACCCTTGTCGCAAt           <  7:29868/148‑1 (MQ=255)                                                                                                                                             gggCCCGGCGGTCGGGAGGGCGATGGCGAATACTGCCGCCATACAGCCCCGGCGTCTTATGTCGGACGATAAA‑CACGCACATTGGCATGTCCCTCACTGAGCAGATGGTGTGCGTGCAAACGGCTCATCACACCCTTGTCGCAATACAg       >  4:234967/1‑149 (MQ=255)                                                                                                                                                 ccGGCGGTCGGGAGGGCGATGGCGAATACTGCCGCCATACAGCCCCGGCGTCTTATGTCGGACGATAAA‑CACGCACATTGGCATGTCCCTCACTGAGCAGATGGTGTGCGTGCAAACGGCTCATCACACCCTTGTCGCAATACAGCAAg   >  7:102054/1‑149 (MQ=255)                                                                                                                                                 ccGGCGGTCGGGAGGGCGATGGCGAATACTGCCGCCATACAGCCCCGGCGTCTTATGTCGGACGATAAA‑CACGCACATTGGCATGTCCCTCACTGAGCAGATGGTGTGCGTGCAAACGGCTCATCACACCCTTGTCGCAATACAGCAAg   >  2:96590/1‑149 (MQ=255)                                                                                                                                                   ggcggTCGGGAGGGCGATGGCGAATACTGCCGCCATACAGCCCCGGCGTCTTATGTCGGACGATAAA‑CACGCACATTGGCATGTCCCTCACTGAGCAGATGGTGTGCGTGCAAACGGCTCATCACACCCTTGTCGCAATACAGCAAGt  >  2:368725/1‑148 (MQ=255)                                                                                                                                                     |                                                                                                                                                   TCGACCAGAGTGGTTTTACCATGGTCAACGTGGGCGATGATGGCGATGTTACGCAGATTTTCGATCACAAGTGTATCTCGATCAGAGGATTCGGTTGCCGCCCAGTGTAGGCGGCCAATATGTACCGTTTAAGGCTCAGCGGGCCCGGCGGTCGGGAGGGCGATGGCGAATACTGCCGCCATACAGCCCCGGCGTCTTATGTCGGACGATAAA‑CACGCACATTGGCATGTCCCTCACTGAGCAGATGGTGTGCGTGCAAACGGCTCATCACACCCTTGTCGCAATACAGCAAGT  >  NC_002947/5749912‑5750205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑