| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,751,740 | C→T | 7.5% | intergenic (‑178/‑293) | thiI ← / → glnA | tRNA sulfurtransferase/glutamine synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,751,740 | 0 | C | T | 7.5% | 146.5 / 2.5 | 54 | intergenic (‑178/‑293) | thiI/glnA | tRNA sulfurtransferase/glutamine synthetase |
| Reads supporting (aligned to +/- strand): ref base C (26/23); new base T (2/2); total (28/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.39e-01 | |||||||||||
AAATGAGGGGCGCGAATTATATCGGAAATTGCTCAGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAG‑‑GCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATCAAAAAACGCATCGTAAGGGTGCACTTTCACCCGCTAACCCAACGCCAAATGCACGCAAAC > NC_002947/5751592‑5751881 | aaaTGAGGGGCGCGAATTATATCGGAAATTGCTCAGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGccc < 1:202182/149‑1 (MQ=255)aaaTGAGGGGCGCGAATTATATCGGAAATTGCTCAGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGccc < 2:86555/149‑1 (MQ=255)aaaTGAGGGGCGCGAATTATATCGGAAATTGCTCAGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGccc < 8:129164/149‑1 (MQ=255) aaTGAGGGGCGCGAATTATATCGGAAATTGCTCAGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCg < 6:162407/149‑1 (MQ=255) aTGAGGGGCGCGAATTATATCGGAAATTGCTCAGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCg > 8:71638/1‑148 (MQ=255) aTGAGGGGCGCGAATTATATCGGAAATTGCTCAGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGt < 5:147203/149‑1 (MQ=255) cgcgAATTATAGCGGAAATTGCTCAGGATATGACCAACTTTTGATCAGCAGCTTTGAGATCAATGCAGAGGCCAGGTTGTGCCGCGCCGGTTCCGGCCTCTTCGCGGGTAAACCCGCGCCTACGGGCTTTGCCCCGCCTGTAGGCGCgg < 5:258615/149‑1 (MQ=21) gcgAATTATATCGGAAATTGCTCAGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCggg > 4:305890/1‑149 (MQ=255) gcgAATTATATCGGAAATTGCTCAGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCggg < 7:71638/149‑1 (MQ=255) tataTCGGAAATTGCTCAGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCTCCTACGGGCTTTGCACCGCCTGTAGgcgc > 4:337576/1‑140 (MQ=37) tataTCGGAAATTGCTCAGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCTCCTACGGGCTTTGCACCGCCTGTAGgcgc < 3:337576/140‑1 (MQ=37) tatCGGAAATTCCTCAGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGTAGAGGCCAGGTTTTGCAGCGCCTGATCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCcg < 8:86710/149‑1 (MQ=255) tCGGAAATTGCTCAGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCcgc < 8:244218/148‑1 (MQ=255) ggAAATTGCTCAGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGACGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACCGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACTCGCGaa < 7:314917/149‑1 (MQ=255) gAAATTGCTCAGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCcgcg > 3:50126/1‑146 (MQ=255) aaaTTGCTCAGGATTTTACAAACTTTTGATCACAAGCTTGTAGATAAATGCAGAGGCCCGGTTTGGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGGGGGTTAACCCGCGAaga < 6:54118/149‑1 (MQ=38) aaaTTGCTCAGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAaga < 6:154748/149‑1 (MQ=255) ttGCTCAGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAG‑‑Gc > 8:274832/1‑149 (MQ=255) tCAGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAG‑‑Gccc > 2:22108/1‑147 (MQ=255) aTTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAG‑‑GCCCTAGAAcc > 6:80229/1‑149 (MQ=255) tttGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAG‑‑GCCCTAGAACCt < 2:216310/149‑1 (MQ=255) aCCAACTTATCATCAGAAGCTTTGACAAAAATGCAGAGGCCAGGTTTTGCAGCCCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAG‑‑GCCCTAGAACCTGGca < 4:305680/149‑1 (MQ=38) cAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAG‑‑GCCCt < 6:117633/136‑1 (MQ=255) cAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAG‑‑GCCCt > 5:117633/1‑136 (MQ=255) cAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAG‑‑GCCCTAGAACCTGGcaca > 5:376672/1‑149 (MQ=255) cAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGACTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGGAAAGCCCGTAGGAGCGGGTTTACACGCGAAGAG‑‑GCCCTAGACCCTGGcac > 8:245577/1‑148 (MQ=255) cTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAG‑‑GCCCTAGAACCTGGCAcacca < 7:114323/149‑1 (MQ=255) ttGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAG‑‑GCCCTAGAACCTGGCACACCACt > 3:346749/1‑148 (MQ=255) tttGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAG‑‑GCCCTAGAACCTGGCACACCACTCACGCCACGCAcc > 1:100645/1‑149 (MQ=255) tttGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAG‑‑GCCCTAGAACCTGGCACACCACTCACGCCACGCAcc > 3:270968/1‑149 (MQ=255) tGAGATAATGGCAGAGGCCCCGTTTTGCCGCGCCTGTCCCGGCCCTTTCGCGGGTAAACCCGCGCATACAGGCGGTGCAAAGCCCTTAGGAGCGGGTTTACCCGCGAAGAG‑‑GCCCTAGAACCTGGCACACCACTCACGCCACGCAcc < 8:328371/147‑1 (MQ=37) aaaTGCAGCGGCCAGTTTTTGCAGCGCGTGTCCCGGCCTTTGCGGGGGAAACCCCGCGCCTACCGGCGGTGAAACGCCGGTAGGCGCGGTTTAACCCGCGAAGAG‑‑GCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGt < 1:183387/149‑1 (MQ=255) gCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAG‑‑GCCCTAGACCCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGAt > 7:79533/1‑149 (MQ=255) gCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAG‑‑GCCCTAGAACCTGGCAcacc < 8:29216/115‑1 (MQ=255) gCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAG‑‑GCCCTAGAACCTGGCAcacc > 7:29216/1‑115 (MQ=255) gCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAG‑‑GCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGAt > 3:268966/1‑149 (MQ=255) gCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTAACCGCGAAGAGTAAACGTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGacac > 1:123571/1‑148 (MQ=38) ccAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAG‑‑GCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATc < 4:346749/149‑1 (MQ=255) ccAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGGTTACCCGCGAAGAG‑‑GCCCGAGAACCTGGCCCCCCGCTGCCGCCACGCCCCCTATTGGTGCGACACGag > 4:60173/1‑147 (MQ=38) gcgcCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAG‑‑GCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATCAAAAAACGCATc < 1:22108/149‑1 (MQ=255) ccTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGGAGGAGCGGGTTTACCCGCGAAGAG‑‑GCCCTAGAACCTGGCACACCACACCCGCCCCGCACCCTATTGGTGCGACACGATCAAAAAACGCATCGt > 2:230366/1‑148 (MQ=255) cTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACCGGCGGTGCAAAGCCCCTAGGAGCGGGTTTACCCGCGAAGAG‑‑GCCCGAGAACCTGGCACACCACTCACGCCACGCACCCAATTGCTGCGACACGATCAAAAACCGCATCGt > 6:77768/1‑147 (MQ=38) cTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAG‑‑GCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATCAAAAAACGCATCGTaa < 3:305890/149‑1 (MQ=255) gTTCCGGCCCTTTCGCGGGTAAACCCGCGCCGACAGGCGGTGCCAAGCCCGTAGGAGCGGGTTTACCCGGGAAGAG‑‑GCCCTTGAACCTGGCACACCCCGACCGCCACGCCCCCTATTGGTGGGACACGCTCAAAAACCGCATCGGAAgg > 8:222004/1‑149 (MQ=37) tCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAG‑‑GCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATCAAAAAACGCATCGTAAGGGTGCACTTTc < 4:50126/147‑1 (MQ=255) ggTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAG‑‑GCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATCAAAAAACGCATCGTAAGGGTGCACTTTCACCCGc > 4:368029/1‑148 (MQ=255) aaCCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAG‑‑GCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATCAAAAAACGCATCGTAAGGGTGCACTTTCACCCGCTAAc < 5:80229/148‑1 (MQ=255) aaCCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAG‑‑GCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATCAAAAAACGCATCGTAAGGGTGCACTTTCACCCGCTAAc < 4:270968/148‑1 (MQ=255) ccGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAG‑‑GCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATCAAAAAACGCATCGTACGGGTGCACTTTCACCCGCTACCCCaa > 7:33162/1‑149 (MQ=255) ccGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAG‑‑GCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATCAAAAAACGCATCGTAAGGGTGCACTTTCACCCGCTAACCCaa > 3:67059/1‑149 (MQ=255) ggccggaaCAGGCGCTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAG‑‑GCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATCAAAAAACGCATCGTAAGGGTGCACTTTCACCCGCTAACCCAAc < 3:172410/142‑1 (MQ=255) cgcgCCTACCGGGGGTGCAAAGCCCGTAGTAGCGGGTTTACCCGCGAAGAG‑‑GCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCTACACGATCAAAAAACGCATCGTAAGGGTGCACTTTCACTCGCTAACCCAAc < 5:62694/149‑1 (MQ=255) gctccTACGGGCTTTGCACCGCCTGTAGGCGCGGGTTTACCCGCGAAAGG‑‑GCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATCAAAAAACGCATCCTAAGGGTGCACTTTCACCCGCTAACCCAACg > 7:255986/4‑149 (MQ=25) gcCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTATACCCGCGAAGAG‑‑GCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATCAAAAAACGCATCGTAAGGGTGCACTTTCACCCGCTAACCCAACGc > 3:257025/1‑148 (MQ=255) gCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAG‑‑GCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATCAAAAAACGCATCGTAAGGGTGCACTTTCACCCGCTAACCCAACGCCAAATGCAc < 4:268966/149‑1 (MQ=255) ggTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAG‑‑GCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATCAAAAAACGCATCGTAAGGGTGCACTTTCACCCGCTAACCCAACGCCAAATGCACg > 7:80066/1‑148 (MQ=255) cAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAG‑‑GCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATCAAAAAACGCATCGTAAGGGTGCACTTTCACCCGCTAACCCAACGCCAAATGCACGCAAAc > 4:25351/1‑149 (MQ=255) | AAATGAGGGGCGCGAATTATATCGGAAATTGCTCAGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAG‑‑GCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATCAAAAAACGCATCGTAAGGGTGCACTTTCACCCGCTAACCCAACGCCAAATGCACGCAAAC > NC_002947/5751592‑5751881 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |