Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,772,819	C→G	8.6%	V48V (GTG→GTC) ‡	betT‑I ←	choline transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,772,819	0	C	G	8.6%	199.4 / 9.8	70	V48V (GTG→GTC) ‡	betT‑I	choline transporter
Reads supporting (aligned to +/- strand):  ref base C (34/29);  new base G (2/4);  total (36/34)
Fisher's exact test for biased strand distribution p-value = 4.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.96e-01

GCTGAAGTCAGGCTGGTCGTCCTTGCCACCCAGCTTGAGGCTGCCGTAGTCGGAGAAAGCCAGGTAGACGACGAACAGCAGGTATCCGCAGATGACCAGCATGTAGTACCAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCCCAGCACCTGGCCGGCGGTTTCGGGTACAGCGATCAGCAAGGCAGTCAGAACAAGGATCATCAGCGCGGAGGTGAAAAACACCACGCGGTTGACCCGTACCCTCTCGGCGGGGGGCTTGGTAAGTGAGGCAGAACTCATTGCA  >  NC_002947/5772678‑5772966                                                                                                                                              |                                                                                                                                                    gCTGAAGTCAGGCTGGTCGTCCTTGCCACCCAGCTTGAGGCTGCCGTAGTCGGAGAAAGCCAGGTAGACGACGAACAGCAGGTATCCGCAGATGACCAGCATGTAGTACCAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCCCa                                                                                                                                              >  6:337957/1‑149 (MQ=255)  cTGAAGTCAGGCTGGTCGTCCTTGCCACCCAGCTTGAGGCTGCCGTAGTCGGAGAAAGCCAGGTAGACGACGAACAGCAGGTATCCGCAGATGACCAGCATGTAGTACCAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCCCAg                                                                                                                                             >  1:220681/1‑149 (MQ=255)   tGAAGTCAGGCTGGTCGTCCTTGCCACCCAGCTTGAGGCTGCCGTAGTCGGAGAAAGCCAGGTAGACGACGAACAGCAGGTATCCGCAGATGACCAGCATGTAGTACCAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCGTGGCCCAg                                                                                                                                             >  2:89221/1‑148 (MQ=255)    gAAGTCAGGCTGGTCGTCCTTGCCACCCAGCTTGAGGCTGCCGTAGTCGGAGAAAGCCAGGTAGACGACGAACAGCAGGTATCCGCAGATGACCAGCATGTAGTACCAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCCCa                                                                                                                                              <  7:40255/146‑1 (MQ=255)    gAAGTCAGGCTGGTCGTCCTTGCCACCCAGCTTGAGGCTGCCGTAGTCGGAGAAAGCCAGGTAGACGACGAACAGCAGGTATCCGCAGATGACCAGCATGTAGTACCAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCCCa                                                                                                                                              >  8:40255/1‑146 (MQ=255)     aaGTCAGGCTGGTCGTCCTTGCCACCCAGCTTGAGGCTGCCGTAGTCGGAGAAAGCCAGGTAGACGACGAACAGCAGGTATCCGCAGATGACCAGCATGTAGTACCAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCGTGGCCCAgaa                                                                                                                                           <  5:182998/148‑3 (MQ=255)     aaGTCAGGCTGGTCGTCCTTGCCACCCAGCTTGAGGCTGCCGTAGTCGGAGAAAGCCAGGTAGACGACGAACAGCAGGTATCCGCAGATGACCAGCATGTAGTACCAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCGTGGCCCAgaa                                                                                                                                           <  1:89221/148‑3 (MQ=255)     aaGTCAGGCTGGTCGTCCTTGCCACCCAGCTTGAGGCTGCCGTAGTCGGAGAAAGCCAGGTAGACGACGAACAGCAGGTATCCGCAGATGACCAGCATGTAGTACCAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCCCAGc                                                                                                                                            <  2:220681/147‑1 (MQ=255)       gTCAGGCTGGTCGTCCTTGCCACCCAGCTTGAGGCTGCCGTAGTCGGAGAAAGCCAGGTAGACGACGAACAGCAGGTATCCGCAGATGACCAGCATGTAGTACCAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCGTGGCCCAgaagtg                                                                                                                                        <  5:291372/149‑6 (MQ=255)        tCAGGCTGGTCGTCCTTGCCACCCAGCTTGAGGCTGCCGTAGTCGGAGAAAGCCAGGTAGACGACGAACAGCAGGTATCCGCAGATGACCAGCATGTAGTACCAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCCCAGCACCTg                                                                                                                                       >  3:10434/1‑149 (MQ=255)        tCAGGCTGGTCGTCCTTGCCACCCAGCTTGAGGCTGCCGTAGTCGGAGAAAGCCAGGTAGACGACGAACAGCAGGTATCCGCAGATGACCAGCATGTAGTACCAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCCCAGCACCTg                                                                                                                                       >  7:180675/1‑149 (MQ=255)          aGGCTGGTCGTCCTTGCCACCCAGCTTGAGGCTGCCGTAGTCGGAGAAAGCCAGGTAGACGACGAACAGCAGGTATCCGCAGATGACCAGCATGTAGTACCAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCCCAGCACCTGGc                                                                                                                                     >  6:169672/1‑149 (MQ=255)          aGGCTGGTCGTCCTTGCCACCCAGCTTGAGGCTGCCGTAGTCGGAGAAAGCCAGGTAGACGACGAACAGCAGGTATCCGCAGATGACCAGCATGTAGTACCAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCCCAGCACCTGGc                                                                                                                                     <  5:289953/149‑1 (MQ=255)           ggCTGGTCGTCCTTGCCACCCAGCTTGAGGCTGCCGTAGTCGGAGAAAGCCAGGTAGACGACGAACAGCAGGTATCCGCAGATGACCAGCATGTAGTACCAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCCCAGCACCTGGcc                                                                                                                                    >  7:86803/1‑149 (MQ=255)            gCTGGTCGTCCTTGCCACCCAGCTTGAGGCTGCCGTAGTCGGAGAAAGCCAGGTAGACGACGAACAGCAGGTATCCGCAGATGACCAGCATGTAGTACCAGGCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCCCAGCACCTGGCcg                                                                                                                                   >  8:338154/1‑149 (MQ=255)                   gtcCTTGCCACCCAGCTTGAGGCTGCCGTAGTCGGAGAAAGCCAGGTAGACGACGAACAGCAGGTATCCGCAGATGACCAGCATGTAGTACCAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCGTGGCCCAgaa                                                                                                                                           >  8:179860/1‑132 (MQ=255)                   gtcCTTGCCACCCAGCTTGAGGCTGCCGTAGTCGGAGAAAGCCAGGTAGACGACGAACAGCAGGTATCCGCAGATGACCAGCATGTAGTACCAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCGTGGCCCAgaa                                                                                                                                           <  7:179860/134‑3 (MQ=255)                   gtcCTTGCCACCCAGCTTGAGGCTGCCGTAGTCGGAGAAAGCCAGGTAGACGACGAACAGCAGGTATCCGCAGATGACCAGCATGTAGTACCAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCCCAGCACCTGGCCGGCGGttt                                                                                                                            >  7:53558/1‑149 (MQ=255)                   gtcCTTGCCACCCAGCTTGAGGCTGCCGTAGTCGGAGAAAGCCAGGTAGACGACGAACAGCAGGTATCCGCAGATGACCAGCATGTAGTACCAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCCCAGCACCTGGCCGGCGGttt                                                                                                                            >  6:24782/1‑149 (MQ=255)                      cTTGCCACCCAGCTTGAGGCTGCCGTAGTCGGAGAAAGCCAGGTAGACGACGAACAGCAGGTATCCGCAGATGACCAGCATGTAGTACCAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCCCAGCACCTGGCCGGCGGTTTCgg                                                                                                                         <  5:132059/149‑1 (MQ=255)                         gCCACCCAGCTTGAGGCTGCCGTAGTCGGAGAAAGCCAGGTAGACGACGAACAGCAGGTATCCGCAGATGACCAGCATGTAGTACCAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCCCAGCACCTGGCCGGCGGTTTCGGGTa                                                                                                                      >  7:100571/1‑149 (MQ=255)                          ccACCCAGCTTGAGGCTGCCGTAGTCGGAGAAAGCCAGGTAGACGACGAACAGCAGGTATCCGCAGATGACCAGCATGTAGTACCAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCCCAGCACCTGGCCGGCGGTTTCGGGTa                                                                                                                      >  5:317007/1‑148 (MQ=255)                             cccAGCTTGAGGCTGCCGTAGTCGGAGAAAGCCAGGTAGACGACGAACAGCAGGTATCCGCAGATGACCAGCATGTAGTACCAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCGCAGCACCTGGCCGGCGGTTTCGGGTACAGc                                                                                                                  <  5:337957/149‑1 (MQ=255)                             cccAGCTTGAGGCTGCCGTAGTCGGAGAAAGCCAGGTAGACGACGAACAGCAGGTATCCGCAGATGACCAGCATGTAGTACCAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCCCAGCACCTGGCCGGCGGTTTCGGGTACAGc                                                                                                                  >  3:274299/1‑149 (MQ=255)                                     gAGGCTGCCGTAGTCGGAGAAAGCCAGGTAGACGACGAACAGCAGGTATCCGCAGATGACCAGCATGTAGTACCAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCCCAGCACCTGGCCGGTGGTTTCGGGTACAGCGATCAGc                                                                                                           >  6:203799/1‑148 (MQ=255)                                      aGGCTGCCGTAGTCGGAGAAAGCCAGGTAGACGACGAACAGCAGGTATCCGCAGATGACCAGCATGTAGTACCAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCCCAGCACCTGGCCGGCGGTTTCGGGTACAg                                                                                                                   >  6:271921/1‑139 (MQ=255)                                      aGGCTGCCGTAGTCGGAGAAAGCCAGGTAGACGACGAACAGCAGGTATCCGCAGATGACCAGCATGTAGTACCAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCCCAGCACCTGGCCGGCGGTTTCGGGTACAg                                                                                                                   <  5:271921/139‑1 (MQ=255)                                          tGCCGTAGTCGGAGAAAGCCAGGTAGACGACGAACAGCTGGTATCCGCAGATGACCAGCATGTAGTACCAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCCCAGCACCTGGCCGGCGGTTTCGGGTACAGCGATCAGCAAgg                                                                                                       >  2:298635/1‑147 (MQ=255)                                              gTAGTCGGAGAAAGCCAGGTAGACGAAGAACAGCAGGTATCCGCAGATGACCAGCATGTAGTACCAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCCCAGCACCTGGCCGGCGGTTTCGGGTACAGCGATCAGCAAGGCAGTCa                                                                                                 <  8:100571/149‑1 (MQ=255)                                                 gTCGGAGAAAGCCAGGTAGACGACGAACAGCAGGTATCCGCAGATGACCAGCATGTAGTACCAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCCCAGCACCTGGCCGGCGGTTTCGGGTACAGCGATCAGCAAGGCAGTCAGaa                                                                                              <  4:313237/149‑1 (MQ=255)                                                 gTCGGAGAAAGCCAGGTAGACGACGAACAGCAGGTATCCGCAGATGACCAGCATGTAGTACCAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCCCAGCACCTGGCCGGCGGTTTCGGGTACAGCGATCAGCAAGGCAGTCAGaa                                                                                              <  8:86803/149‑1 (MQ=255)                                                             cAGGTAGACGACGAACAGCAGGTATCCGCAGATGACCAGCATGTAGTACCAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCCCAGCACCTGGCCGGCGGTTTCGGGTACa                                                                                                                    <  4:38472/115‑1 (MQ=255)                                                             cAGGTAGACGACGAACAGCAGGTATCCGCAGATGACCAGCATGTAGTACCAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCCCAGCACCTGGCCGGCGGTTTCGGGTACa                                                                                                                    >  3:38472/1‑115 (MQ=255)                                                             cAGGTAGACGACGAACAGCAGGTATCCGCAGATGACAAGCATGTAGTACCAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCCCAGCACCTGGCCGGCGGTTTCGGGTACAGCGATCAGCACGGCAGTCAGAACAAGCAtcatca                                                                                  >  8:297896/1‑149 (MQ=255)                                                                      gacgaACAGCAGGTATCCGCAGATGACCAGCATGTAGTACCAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCCCAGCACCTGGCCGGCGGTTTCGGGTACAGCGATCAGCAAGGCAGTCAGAACAAGGATCATCAGCGCGGAgg                                                                         >  2:271649/1‑149 (MQ=255)                                                                               cagGTATCCGCAGATGACCAGCATGTAGTACCAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACTCCCAGCACCTGGCCGGTGGTTTCGGGTACAGCGATCAGCAAGGCAGTCAGAACAAGGATCATCAGCGCGGAGGTGAAAAaca                                                                <  5:203799/149‑1 (MQ=255)                                                                                       cGCAGATGACCAGCATGTAGTACCAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCCCAGCACCTGGCCGGCGGTTTCGGGTACAGCGATCAGCAAGGCAGTCAGAACAAGGATCATCAGCGCGGAGGTGAAAAACACCACGCgg                                                        >  1:149519/1‑149 (MQ=255)                                                                                       cGCAGATGACCAGCATGTAGTACCAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCCCAGCACCTGGCCGGCGGTTTCGGGTACAGCGATCAGCAAGGCAGTCAGAACAAGGATCATCAGCGCGGAGGTGAAAAACACCACGCgg                                                        >  3:329498/1‑149 (MQ=255)                                                                                             tGACCAGCATGTAGTACCAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCCCAGCACCTGGCCGGCGGTTTCGGGTACAGCGATCAGCAAGGCAGTCAGAACAAGGATCATCAGCGCGGAGGTGAAAAACACCACGCGGTTGAcc                                                  <  2:7664/149‑1 (MQ=255)                                                                                                ccAGCATGTAGTACCAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCCCAGCACCTGGCCGGCGGTTTCGGGTACAGCGATCAGCAAGGCAGTCAGAACAAGGATCATCAGCGCGGAGGTGAAAAACACCACGCGGTTGACCCGt                                               <  5:169672/149‑1 (MQ=255)                                                                                                   gCATGTAGTACCAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCCCAGCACCTGGCCGGCGGTTTCGGGTACAGCGATCAGCAAGGCAGTCAGAACAAGGATCATCAGCGCGGAGGTGAAAAACACCACGCGGTTGACCCGTAcc                                            >  5:296452/1‑149 (MQ=255)                                                                                                          gtaCCAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCCCAGCACCTGGCCGGCGGTTTCGGGTACAGCGATCAGCAAGGCAGTCAGAACAAGGATCATCAGCGCGGAGGTGAAAAACACCACGCGGTTGACCCGTACCCTCTcg                                      <  4:10434/148‑1 (MQ=255)                                                                                                          gtaCCAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCCCAGCACCTGGCCGGCGGTTTCGGGTACAGCGATCAGCAAGGCAGTCAGAACAAGGATCATCAGCGCGGAGGTGAAAAACACCACGCGGTTGACCCGTACCCTCTcg                                      <  4:274299/148‑1 (MQ=255)                                                                                                          ctaCCAGCCAAACGTGCGCGTCAGCCACTTCTGGGCCACTCCCAGCACCTGGCCGGCGGTTTCGGGTACAGCGATCAGCAAGGCAGTCAGAACAAGGATCATCAGCGCGGAGGTGAAAAACACCACGCGGTTGACCCGTACCCTCTcg                                      <  8:180675/147‑1 (MQ=255)                                                                                                            aCCAGCCACAGGTGCGCGGCAGCCACTTCTGGGCAACGCCCAGCACCTGGCCGGCGGTTTCGGGTACAGCGATCAGCAAGGCAGTCAGAACAAGGATCATCAGCGCGGAGGTGAAAAACACCACGCGGTTGACCCGTACCCTCTcggcg                                   <  8:327992/149‑1 (MQ=255)                                                                                                             ccAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCCCAGCACCTGGCCGGCGGTTTCGGGTACAGCGATCAGCAAGGCAGTCAGAACAAGGatc                                                                                      >  2:279717/1‑97 (MQ=255)                                                                                                             ccAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCCCAGCACCTGGCCGGCGGTTTCGGGTACAGCGATCAGCAAGGCAGTCAGAACAAGGATCATCAGCGCGGAGGTGAAAAACACCACGCGGTTGACCCGTACCCTCTcggcgg                                  <  6:38943/149‑1 (MQ=255)                                                                                                             ccAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCCCAGCACCTGGCCGGCGGTTTCGGGTACAGCGATCAGCAAGGCAGTCAGAACAAGGAGCATCAGCGCGGAGGTGAAAAACCCCCCGCGGTTGACCCGTACCCTCgcggcg                                   >  7:327992/1‑148 (MQ=255)                                                                                                              cAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCCCAGCACCTGGCCGGCTGTTTCGGGTACAGCGATCAGCAAGGCAGTCAGAACACGGATCATCAGCGCGGAGGTGAAAAACACCACGCGGTTGACCCGTACCCTCTCGGCggg                                 <  5:282688/149‑1 (MQ=255)                                                                                                              cAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCCCAGCACCTGGCCGGCGGTTTCGGGTACAGCGATCAGCAAGGCAGTCAGAACCAGGATCATCAGCGCGGAGGTGAAAAACACCACGCGGTTGACCCGTACCCTCTCGGCggg                                 <  1:298635/149‑1 (MQ=255)                                                                                                              cAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCCCAGCACCTGGCCGGCGGTTTCGGGTACAGCGATCAGCAAGGCAGTCAGAACAAGGATCATCAGCGCGGAGGTG‑AAAACACCACGCGGTTGACCCGTACCCTCTCGGCgggg                                >  3:35286/1‑149 (MQ=255)                                                                                                               aGCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCCCAGCACCTGGCCGGCGGTTTCGGGTACAGCGATCAGCAAGGCAGTCAGAACAAGGATCATCAGCGCGGAGGTGAAAAACACCACGCGGTTGACCCGTACCCTCTCGGCgggg                                <  6:317007/149‑1 (MQ=255)                                                                                                                gCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCCCAGCACCTGGCCGGCGGTTTCGGGTACAGCGATCAGCAAGGCAGTCAGAACAAGGATCATCAGCGCGGAGGTGAAAAACACCACGCGGTTGACCCGTACCCTCTCGGCggggg                               <  5:24782/149‑1 (MQ=255)                                                                                                                   aaaGGTGCGCGTCAGCCACTTCTGGGCCACGCCCAGCACCTGGCCGGCGGTTTCGTGTACAGCGATCAGCAAGGCAGTCAGAACAAGGATCATCAGCGCGGAGGTGAAAAACACCACGCGGTTGACCCGTACCCTCTCGGCGGCGGGCt                            >  8:284118/1‑149 (MQ=255)                                                                                                                   aaaGGTGCGCGTCAGCAACTTCTGGGCCACGCCCAGCAGCTGGCCGGCGGTTTCGGGTACAGCGATCAGCAAGGCAGTCAGAAAATGGATCATCAGCGCGGAGGTGAAAAACCCCACGCGGTTGACCCGTCCCCGGTCGGCGGGGGGcg                            >  6:272517/1‑148 (MQ=255)                                                                                                                    aaGGTGCGCGTCAGCCACTTCTGGGCCACGCCCAGCACCTGGCCGGCGGTTTCGGGTACAGCGATCAGCAAGGCAGTCAGAACAAGGATCATCAGCGCGGAGGTGAAAAACACCACGCGGTTGACCCGTACCCTCTCGGCGGGGGGCtt                           >  2:32788/1‑149 (MQ=255)                                                                                                                       gTGCGCGTCAGCCACTTCTGGGCCACGCCCAGCACCTGGCCGGCGGTTTCGGGTACAGCGATCAGCAAGGCAGTCAGCACAAGGATCATCAGCGCGGAGGTGAAAAACACCACGCGGTTGACCCGTACCCTCTCGGCGGGGGGCTTgg                         >  5:250018/1‑148 (MQ=255)                                                                                                                       gTGCGCGTCAGCCACTTCTGGGCCACGCCCAGCACCTGGCCGGCGGTTTCGGGTACAGCGATCAGCAAGGCAGTCAGAACAAGGATCATCAGCGCGGAGGTGAAAAACACCACGCGGTTGACCCGTACCCTCTCGGCGGGGGGCTTGGt                        <  8:53558/149‑1 (MQ=255)                                                                                                                          cgcgTCAGCCACTTCTGGGCCACGCCCAGCACCTGGCCGGCGGTTTCGGGTACAGCGATCAGCAAGGCAGTCAGAACAAGGATCATCAGCGCGGAGGTG‑AAAACACCACGCGGTTGACCCGTACCCTCTCGGCGGGGGGCTTGGTAAGt                    <  4:35286/149‑1 (MQ=255)                                                                                                                           gcgTCAGCCACTTCTTGGCCACGCCCAGCACCTGGCCGGCGGTTTCGGGTACAGCGATCAGCAAGGCAGTCAGAACAAGGATCATCAGCGCGGAGGTGAAAAACACCACGCGGTTGACCCGTACCCTCTCGGCGGGGGGCTTGGTAAGt                    <  6:296452/149‑1 (MQ=255)                                                                                                                           gcgTCAGCCACTTCTGGGCCACGCCCAGCACCTGGCCGGCGGTTTCGGGTACAGCGATCAGCAAGGCAGTCAGAACAAGGATCATCAGCGCGGAGGTGAAAAACACCACGCGGTTGACCCGTACCCTCTCGGCGGGGGGCTTGGTAAGt                    >  7:292740/1‑149 (MQ=255)                                                                                                                                 gCCACTTCTGGGCCACGCCCAGCACCTGGCCGGCGGTTTCGGGTACAGCGATCAGCAAGGCAGTCAg                                                                                                >  6:191158/1‑67 (MQ=255)                                                                                                                                 gCCACTTCTGGGCCACGCCCAGCACCTGGCCGGCGGTTTCGGGTACAGCGATCAGCAAGGCAGTCAGAACAAGGATCATCAGCGCGGAGGTGAAAAACACCACGCGGTTGACCCGTACCCTCTCGGCGGGGGGCTTGGTAAGTGAGGCa              <  2:338597/149‑1 (MQ=255)                                                                                                                                  ccACTTCTGGGCCACGCCCAGCACCTGGCCGGCGGTTTCGGGTACAGCGATCAGCAAGGCAGTCAGAACAAGGATCATCAGCGCGGAGGTGAAAAACACCACGCGGTTGACCCGTACCCTCTCGGCGGGGGGCTTGGTAAGTGAgg                >  5:155753/1‑146 (MQ=255)                                                                                                                                   cACTTCTGGGCCACGCCCAGCACCTGGCCGGCGGTTTCGGGTACAGCGATCAGCAAGGCAGTCAGAACAAGGATCATCAGCGCGGAGGTGAAAAACACCACGCGGTTGACCCGTACACTCTCGGCGGGGGGCTTGGTAAGTGAGGCAg             >  6:94249/1‑148 (MQ=255)                                                                                                                                    aCTTCTGGGCCACGCCCAGCACCTGGCCGGCGGTTTCGGGTACAGCGATCAGCAAGGCATTCAGAACAAGGATCATCAGCGCGGAGGTGAAAAACACCACGCGGTTGACCCGTACCCTCTCGGCGGGGGGCTTGGTAAGTGAGGCAGaa           <  7:338154/149‑1 (MQ=255)                                                                                                                                    aCTTCTGGGCCACGCCCAGCACCTGGCCGGCGGTTTCGGGTACAGCGATCAGCAAGGCAGTCAGAACAAGGATCATCAGCGCGGAGGTGAAAAACACCACGCGGTTGACCCGTACCCTCTCGGCGGGGGGCTTGGTAAGTGAGGCAGaa           <  1:173639/149‑1 (MQ=255)                                                                                                                                           ggCCACGCCCAGCACCTGGCCGGCGGTTTCGGGTACAGCGATCAGCAAGGCAGTCAGAACAAGGATCATCAGCGCGGAGGTGAAAAACACCACGCGGTTGACCCGTACCCTCTCGGCGGGGGGCTTGGTAAGTGAGGCAGAACTCAtt     <  7:297896/148‑1 (MQ=255)                                                                                                                                            gCCACGCCCAGCACCTGGCCGGCGGTTTCGGGTACAGCGATCAGCAAGGCAGTCAGAACAAGGATCATCAGCGCGGAGGTGAAAAACACCACGCGGTTGACCCGTa                                              >  6:311203/1‑106 (MQ=255)                                                                                                                                            gCCACGCCCAGCACCTGGCCGGCGGTTTCGGGTACAGCGATCAGCAAGGCAGTCAGAACAAGGATCATCAGCGCGGAGGTGAAAAACACCACGCGGTTGACCCGTa                                              <  5:311203/106‑1 (MQ=255)                                                                                                                                             ccACGCCCAGCACCTGGCCGGCGGTTTCGGGTACAGCGATCAGCAAGGCAGTCAGAACAAGGATCATCAGCGCGGAGGTGAAAAACACCACGCGGTTGACCCGTACCCTCTCGGCGGGGGGCTTGGTAAGTGAGGCAGAACTCATTGCa  >  5:35450/1‑149 (MQ=255)                                                                                                                                              |                                                                                                                                                    GCTGAAGTCAGGCTGGTCGTCCTTGCCACCCAGCTTGAGGCTGCCGTAGTCGGAGAAAGCCAGGTAGACGACGAACAGCAGGTATCCGCAGATGACCAGCATGTAGTACCAGCCAAAGGTGCGCGTCAGCCACTTCTGGGCCACGCCCAGCACCTGGCCGGCGGTTTCGGGTACAGCGATCAGCAAGGCAGTCAGAACAAGGATCATCAGCGCGGAGGTGAAAAACACCACGCGGTTGACCCGTACCCTCTCGGCGGGGGGCTTGGTAAGTGAGGCAGAACTCATTGCA  >  NC_002947/5772678‑5772966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑