Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,792,841 A→C 5.2% C164G (TGC→GGC)  gltD ← glutamate synthase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,792,8410AC 5.2% 288.1 / 4.1 91C164G (TGC→GGC) gltDglutamate synthase subunit beta
Reads supporting (aligned to +/- strand):  ref base A (44/42);  new base C (3/2);  total (47/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCATGCCGGTGAACACTTCACGGCGGTTGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACCGCGCCGAAACC  >  NC_002947/5792694‑5792985
                                                                                                                                                   |                                                                                                                                                
ccATGCCGGTGAACACTTCACGGCGGTTGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAg                                                                                                                                                 >  2:321746/1‑149 (MQ=255)
ccATGCCGGTGAACACTTCACGGCGGTTGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAg                                                                                                                                                 <  6:114659/149‑1 (MQ=255)
 cATGCCGGTGAACACTTCACGGCGGTTGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGc                                                                                                                                                >  5:335191/1‑149 (MQ=255)
        gTGAACACTTCACGGCGGTTGCTGAGCACAGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGcc                                                                                                                                         >  4:45971/1‑149 (MQ=255)
         tGACCACTTCACGGCGGTTGCTGCGCACGGTCTTTTCCAGCTTGAAGTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGccc                                                                                                                                        <  3:306187/149‑1 (MQ=255)
               cTTCACGGCGGTTGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCccgccg                                                                                                                                    <  1:241211/147‑1 (MQ=255)
               cTTCACGGCGGTTGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCccgccg                                                                                                                                    <  1:246501/147‑1 (MQ=255)
               cTTCACGGCGGTTGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCccgccg                                                                                                                                    >  2:246501/1‑147 (MQ=255)
               cTTCACGGCGGTTGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCccgccg                                                                                                                                    >  2:241211/1‑147 (MQ=255)
                ttCACGGCGGTTGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCgccgggc                                                                                                                                 >  4:272960/1‑149 (MQ=255)
                ttCACGGCGGTTGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCgccgggc                                                                                                                                 >  3:261795/1‑149 (MQ=255)
                   aCGGCGGTTGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCgccgggccgg                                                                                                                              <  6:301045/149‑1 (MQ=255)
                    cggcggTTGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGCTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGgc                                                                                                                             >  4:41539/1‑149 (MQ=255)
                         gTTGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCga                                                                                                                        <  7:96696/149‑1 (MQ=255)
                          ttGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCCGGCCCGGCGGGCCTGGGCTGCGCCgac                                                                                                                       <  3:74118/149‑2 (MQ=38)
                             cTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCAGCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCgatgat                                                                                                                    >  5:261633/1‑149 (MQ=255)
                             cTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCgatgat                                                                                                                    <  3:334896/149‑1 (MQ=255)
                              tGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGatgatg                                                                                                                   >  3:212042/1‑149 (MQ=255)
                              tGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGatgatg                                                                                                                   >  3:361573/1‑149 (MQ=255)
                                aGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGcc                                                                                                                                               >  7:182377/1‑119 (MQ=255)
                                aGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGcc                                                                                                                                               <  8:182377/119‑1 (MQ=255)
                                aGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATgg                                                                                                                  <  7:179688/148‑1 (MQ=255)
                                    cGGTC‑TTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAAcgc                                                                                                            >  1:363458/1‑149 (MQ=255)
                                     ggTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAAcgc                                                                                                            >  6:63221/1‑149 (MQ=255)
                                     ggTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAAcgc                                                                                                            >  5:180408/1‑149 (MQ=255)
                                     ggTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACATCGGCGCCGGCCCGGCGGGCCTGGGCTGCGCCgacg                                                                                                                      <  6:109388/139‑3 (MQ=37)
                                     ggTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACATCGGCGCCGGCCCGGCGGGCCTGGGCTGCGCCgacg                                                                                                                      >  5:109388/1‑137 (MQ=37)
                                      gTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCccgc                                                                                                                                      <  7:53335/122‑1 (MQ=255)
                                       tCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACgcgc                                                                                                          >  4:20521/1‑149 (MQ=255)
                                        cTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCt                                                                                                         <  3:272960/149‑1 (MQ=255)
                                         ttttCCAGCTTGAACTCGGGGATATCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCtt                                                                                                        >  5:305496/1‑149 (MQ=255)
                                           ttCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCtt                                                                                                        <  2:61776/147‑1 (MQ=255)
                                              cAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCgg                                                                                                   >  8:166033/1‑149 (MQ=255)
                                                      aCTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTg                                                                                           <  3:45971/149‑1 (MQ=255)
                                                       cTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGa                                                                                          <  3:20521/149‑1 (MQ=255)
                                                        tCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGAc                                                                                         >  5:211947/1‑149 (MQ=255)
                                                          ggggATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGAc                                                                                         <  7:39058/147‑1 (MQ=255)
                                                           gggCTACCGAAGGGCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGGCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCtt                                                                                      <  5:374290/149‑1 (MQ=255)
                                                           gggATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCGAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCtt                                                                                      <  2:144993/149‑1 (MQ=255)
                                                            ggATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAAc                                                                                                              <  4:123213/124‑1 (MQ=255)
                                                            ggATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAAc                                                                                                              >  3:123213/1‑124 (MQ=255)
                                                            ggATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTg                                                                                     <  5:270595/149‑1 (MQ=255)
                                                             gATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTgg                                                                                    <  4:261795/149‑1 (MQ=255)
                                                                  cGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATg                                                                               >  1:277135/1‑149 (MQ=255)
                                                                  cGAAGGTCAGCAGGCCACCGATATCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCTGCTCCGATGATGGCAACGCGCTTGCCCGTTGGCTTGACCTTGGACATg                                                                               >  5:154086/1‑149 (MQ=255)
                                                                     aGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTcc                                                                            <  3:58674/149‑1 (MQ=255)
                                                                       gTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCgg                                                                          <  6:261633/149‑1 (MQ=255)
                                                                               gCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGc                                                                  >  6:87193/1‑149 (MQ=255)
                                                                                         tcAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCg                                                          >  3:367139/1‑147 (MQ=255)
                                                                                           aGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAgg                                                      >  3:107/1‑149 (MQ=255)
                                                                                           aGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAgg                                                      >  3:84992/1‑149 (MQ=255)
                                                                                           aGGGTGTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAg                                                       <  4:53002/148‑1 (MQ=255)
                                                                                             ggTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCg                                                    <  4:367139/149‑1 (MQ=255)
                                                                                                       gAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTg                                           <  4:212042/148‑1 (MQ=255)
                                                                                                       gAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGa                                          >  7:286083/1‑149 (MQ=255)
                                                                                                       gAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGa                                          >  3:216498/1‑149 (MQ=255)
                                                                                                       gAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGa                                          <  7:166033/149‑1 (MQ=255)
                                                                                                       gAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGa                                          <  6:180408/149‑1 (MQ=255)
                                                                                                         acacCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATg                                        >  5:11882/1‑149 (MQ=255)
                                                                                                           accaccGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTg                                           <  4:47729/144‑1 (MQ=255)
                                                                                                           accaccGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGaagat                                      >  3:47729/1‑145 (MQ=255)
                                                                                                            ccaccGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTAc                                     <  4:216498/149‑1 (MQ=255)
                                                                                                            ccaccGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTAc                                     <  2:363458/149‑1 (MQ=255)
                                                                                                            ccaccGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTAc                                     <  6:335191/149‑1 (MQ=255)
                                                                                                             caccGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGCTGGCAACGCGCTTGCCGGTTGGCTGGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACt                                    >  5:357262/1‑149 (MQ=255)
                                                                                                             caccGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAAGGCGCTTGCCGGTTGGCTTGACCTTGGACTTGTCCGGACGCCAGCCCATGGCGAAGGCGGAGTCGGTGATGTACt                                    <  1:268701/149‑1 (MQ=255)
                                                                                                             caccGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACt                                    >  3:341343/1‑149 (MQ=255)
                                                                                                             caccGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACt                                    >  4:302468/1‑149 (MQ=255)
                                                                                                              accGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACtt                                   <  3:322090/149‑1 (MQ=255)
                                                                                                              accGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACtt                                   <  4:322892/149‑1 (MQ=255)
                                                                                                              accGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACtt                                   >  4:322090/1‑149 (MQ=255)
                                                                                                                   ggTAACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGAt                                         <  5:268631/138‑1 (MQ=255)
                                                                                                                   ggTAACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGAt                                         >  6:268631/1‑138 (MQ=255)
                                                                                                                         cccGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGc                        >  1:1869/1‑149 (MQ=255)
                                                                                                                         cccGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGc                        <  5:63221/149‑1 (MQ=255)
                                                                                                                          ccGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGcc                       >  7:149398/1‑149 (MQ=255)
                                                                                                                           cGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCAGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGcc                       >  6:215695/1‑148 (MQ=255)
                                                                                                                             gcgcgcACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGAt                    <  2:277135/149‑1 (MQ=255)
                                                                                                                                cgcACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGt                 <  4:84992/149‑1 (MQ=255)
                                                                                                                                     cAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACcg            >  2:90620/1‑149 (MQ=255)
                                                                                                                                     cAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACcg            >  1:80424/1‑149 (MQ=255)
                                                                                                                                       gCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACcgcg          >  8:5219/1‑149 (MQ=255)
                                                                                                                                         tcatCGGCGCCGGCCCGGCGGGCCTGGGCTGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGAACTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACCGCGcc        >  5:266868/4‑149 (MQ=38)
                                                                                                                                         aCGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCAcc             <  2:218328/144‑1 (MQ=255)
                                                                                                                                         aCGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCAcc             >  1:218328/1‑144 (MQ=255)
                                                                                                                                         aCGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACCGCGcc        <  8:286083/149‑1 (MQ=255)
                                                                                                                                           gTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACCGCGCCg       >  7:293836/1‑148 (MQ=255)
                                                                                                                                             cGGCGCCGGCCCGGCGGGCCTGGGCTGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACCGCGCCGaaa    >  5:16446/1‑149 (MQ=255)
                                                                                                                                              ggCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACCGCGCCGAAAc   >  1:143450/1‑149 (MQ=255)
                                                                                                                                               gggcAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACCGCGCCGAAAcc  <  6:226503/147‑1 (MQ=255)
                                                                                                                                               gcgcAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACCGCGCCGAAAcc  <  3:41539/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CCATGCCGGTGAACACTTCACGGCGGTTGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACCGCGCCGAAACC  >  NC_002947/5792694‑5792985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑