Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,792,843 C→G 5.7% G163A (GGC→GCC)  gltD ← glutamate synthase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,792,8430CG 5.7% 280.7 / 4.2 86G163A (GGC→GCC) gltDglutamate synthase subunit beta
Reads supporting (aligned to +/- strand):  ref base C (41/40);  new base G (3/2);  total (44/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

CATGCCGGTGAACACTTCACGGCGGTTGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACCGCGCCGAAACCGTCGTT  >  NC_002947/5792695‑5792991
                                                                                                                                                    |                                                                                                                                                    
cATGCCGGTGAACACTTCACGGCGGTTGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGc                                                                                                                                                      >  5:335191/1‑149 (MQ=255)
       gTGAACACTTCACGGCGGTTGCTGAGCACAGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGcc                                                                                                                                               >  4:45971/1‑149 (MQ=255)
        tGACCACTTCACGGCGGTTGCTGCGCACGGTCTTTTCCAGCTTGAAGTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGccc                                                                                                                                              <  3:306187/149‑1 (MQ=255)
              cTTCACGGCGGTTGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCccgccg                                                                                                                                          <  1:241211/147‑1 (MQ=255)
              cTTCACGGCGGTTGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCccgccg                                                                                                                                          <  1:246501/147‑1 (MQ=255)
              cTTCACGGCGGTTGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCccgccg                                                                                                                                          >  2:246501/1‑147 (MQ=255)
              cTTCACGGCGGTTGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCccgccg                                                                                                                                          >  2:241211/1‑147 (MQ=255)
               ttCACGGCGGTTGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCgccgggc                                                                                                                                       >  4:272960/1‑149 (MQ=255)
               ttCACGGCGGTTGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCgccgggc                                                                                                                                       >  3:261795/1‑149 (MQ=255)
                  aCGGCGGTTGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCgccgggccgg                                                                                                                                    <  6:301045/149‑1 (MQ=255)
                   cggcggTTGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGCTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGgc                                                                                                                                   >  4:41539/1‑149 (MQ=255)
                        gTTGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCga                                                                                                                              <  7:96696/149‑1 (MQ=255)
                         ttGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCCGGCCCGGCGGGCCTGGGCTGCGCCgac                                                                                                                             <  3:74118/149‑2 (MQ=38)
                            cTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCAGCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCgatgat                                                                                                                          >  5:261633/1‑149 (MQ=255)
                            cTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCgatgat                                                                                                                          <  3:334896/149‑1 (MQ=255)
                             tGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGatgatg                                                                                                                         >  3:361573/1‑149 (MQ=255)
                             tGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGatgatg                                                                                                                         >  3:212042/1‑149 (MQ=255)
                               aGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGcc                                                                                                                                                     <  8:182377/119‑1 (MQ=255)
                               aGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGcc                                                                                                                                                     >  7:182377/1‑119 (MQ=255)
                               aGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATgg                                                                                                                        <  7:179688/148‑1 (MQ=255)
                                   cGGTC‑TTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAAcgc                                                                                                                  >  1:363458/1‑149 (MQ=255)
                                    ggTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAAcgc                                                                                                                  >  5:180408/1‑149 (MQ=255)
                                    ggTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAAcgc                                                                                                                  >  6:63221/1‑149 (MQ=255)
                                    ggTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACATCGGCGCCGGCCCGGCGGGCCTGGGCTGCGCCgacg                                                                                                                            >  5:109388/1‑137 (MQ=37)
                                    ggTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACATCGGCGCCGGCCCGGCGGGCCTGGGCTGCGCCgacg                                                                                                                            <  6:109388/139‑3 (MQ=37)
                                     gTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCccgc                                                                                                                                            <  7:53335/122‑1 (MQ=255)
                                      tCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACgcgc                                                                                                                >  4:20521/1‑149 (MQ=255)
                                       cTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCt                                                                                                               <  3:272960/149‑1 (MQ=255)
                                        ttttCCAGCTTGAACTCGGGGATATCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCtt                                                                                                              >  5:305496/1‑149 (MQ=255)
                                          ttCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCtt                                                                                                              <  2:61776/147‑1 (MQ=255)
                                             cAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCgg                                                                                                         >  8:166033/1‑149 (MQ=255)
                                                     aCTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTg                                                                                                 <  3:45971/149‑1 (MQ=255)
                                                      cTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGa                                                                                                <  3:20521/149‑1 (MQ=255)
                                                       tCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGAc                                                                                               >  5:211947/1‑149 (MQ=255)
                                                         ggggATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGAc                                                                                               <  7:39058/147‑1 (MQ=255)
                                                          gggCTACCGAAGGGCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGGCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCtt                                                                                            <  5:374290/149‑1 (MQ=255)
                                                          gggATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCGAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCtt                                                                                            <  2:144993/149‑1 (MQ=255)
                                                           ggATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAAc                                                                                                                    <  4:123213/124‑1 (MQ=255)
                                                           ggATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAAc                                                                                                                    >  3:123213/1‑124 (MQ=255)
                                                           ggATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTg                                                                                           <  5:270595/149‑1 (MQ=255)
                                                            gATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTgg                                                                                          <  4:261795/149‑1 (MQ=255)
                                                                 cGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATg                                                                                     >  1:277135/1‑149 (MQ=255)
                                                                 cGAAGGTCAGCAGGCCACCGATATCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCTGCTCCGATGATGGCAACGCGCTTGCCCGTTGGCTTGACCTTGGACATg                                                                                     >  5:154086/1‑149 (MQ=255)
                                                                    aGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTcc                                                                                  <  3:58674/149‑1 (MQ=255)
                                                                      gTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCgg                                                                                <  6:261633/149‑1 (MQ=255)
                                                                              gCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGc                                                                        >  6:87193/1‑149 (MQ=255)
                                                                                        tcAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCg                                                                >  3:367139/1‑147 (MQ=255)
                                                                                          aGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAgg                                                            >  3:107/1‑149 (MQ=255)
                                                                                          aGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAgg                                                            >  3:84992/1‑149 (MQ=255)
                                                                                          aGGGTGTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAg                                                             <  4:53002/148‑1 (MQ=255)
                                                                                            ggTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCg                                                          <  4:367139/149‑1 (MQ=255)
                                                                                                      gAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTg                                                 <  4:212042/148‑1 (MQ=255)
                                                                                                      gAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGa                                                <  6:180408/149‑1 (MQ=255)
                                                                                                      gAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGa                                                <  7:166033/149‑1 (MQ=255)
                                                                                                      gAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGa                                                >  3:216498/1‑149 (MQ=255)
                                                                                                      gAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGa                                                >  7:286083/1‑149 (MQ=255)
                                                                                                        acacCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATg                                              >  5:11882/1‑149 (MQ=255)
                                                                                                          accaccGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTg                                                 <  4:47729/144‑1 (MQ=255)
                                                                                                          accaccGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGaagat                                            >  3:47729/1‑145 (MQ=255)
                                                                                                           ccaccGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTAc                                           <  4:216498/149‑1 (MQ=255)
                                                                                                           ccaccGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTAc                                           <  2:363458/149‑1 (MQ=255)
                                                                                                           ccaccGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTAc                                           <  6:335191/149‑1 (MQ=255)
                                                                                                            caccGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGCTGGCAACGCGCTTGCCGGTTGGCTGGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACt                                          >  5:357262/1‑149 (MQ=255)
                                                                                                            caccGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAAGGCGCTTGCCGGTTGGCTTGACCTTGGACTTGTCCGGACGCCAGCCCATGGCGAAGGCGGAGTCGGTGATGTACt                                          <  1:268701/149‑1 (MQ=255)
                                                                                                            caccGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACt                                          >  4:302468/1‑149 (MQ=255)
                                                                                                            caccGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACt                                          >  3:341343/1‑149 (MQ=255)
                                                                                                             accGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACtt                                         <  3:322090/149‑1 (MQ=255)
                                                                                                             accGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACtt                                         <  4:322892/149‑1 (MQ=255)
                                                                                                             accGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACtt                                         >  4:322090/1‑149 (MQ=255)
                                                                                                                  ggTAACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGAt                                               <  5:268631/138‑1 (MQ=255)
                                                                                                                  ggTAACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGAt                                               >  6:268631/1‑138 (MQ=255)
                                                                                                                        cccGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGc                              >  1:1869/1‑149 (MQ=255)
                                                                                                                        cccGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGc                              <  5:63221/149‑1 (MQ=255)
                                                                                                                         ccGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGcc                             >  7:149398/1‑149 (MQ=255)
                                                                                                                          cGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCAGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGcc                             >  6:215695/1‑148 (MQ=255)
                                                                                                                            gcgcgcACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGAt                          <  2:277135/149‑1 (MQ=255)
                                                                                                                               cgcACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGt                       <  4:84992/149‑1 (MQ=255)
                                                                                                                                    cAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACcg                  >  1:80424/1‑149 (MQ=255)
                                                                                                                                    cAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACcg                  >  2:90620/1‑149 (MQ=255)
                                                                                                                                      gCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACcgcg                >  8:5219/1‑149 (MQ=255)
                                                                                                                                        tcatCGGCGCCGGCCCGGCGGGCCTGGGCTGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGAACTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACCGCGcc              >  5:266868/4‑149 (MQ=38)
                                                                                                                                        aCGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCAcc                   <  2:218328/144‑1 (MQ=255)
                                                                                                                                        aCGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCAcc                   >  1:218328/1‑144 (MQ=255)
                                                                                                                                        aCGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACCGCGcc              <  8:286083/149‑1 (MQ=255)
                                                                                                                                          gTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACCGCGCCg             >  7:293836/1‑148 (MQ=255)
                                                                                                                                            cGGCGCCGGCCCGGCGGGCCTGGGCTGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACCGCGCCGaaa          >  5:16446/1‑149 (MQ=255)
                                                                                                                                             ggCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACCGCGCCGAAAc         >  1:143450/1‑149 (MQ=255)
                                                                                                                                              gggcAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACCGCGCCGAAAcc        <  6:226503/147‑1 (MQ=255)
                                                                                                                                              gcgcAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACCGCGCCGAAAcc        <  3:41539/149‑1 (MQ=255)
                                                                                                                                                    cccAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACCGCGCCGAAACCGTCGtt  <  3:302468/149‑1 (MQ=255)
                                                                                                                                                    cccAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACCGCGCCGAAACCGTCGtt  >  6:73335/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CATGCCGGTGAACACTTCACGGCGGTTGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACCGCGCCGAAACCGTCGTT  >  NC_002947/5792695‑5792991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑