Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,792,846 A→C 5.2% L162R (CTG→CGG)  gltD ← glutamate synthase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,792,8460AC 5.2% 281.6 / 4.4 91L162R (CTG→CGG) gltDglutamate synthase subunit beta
Reads supporting (aligned to +/- strand):  ref base A (45/41);  new base C (3/2);  total (48/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01

GTGAACACTTCACGGCGGTTGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACCGCGCCGAAACCGTCGTTCAG  >  NC_002947/5792702‑5792994
                                                                                                                                                |                                                                                                                                                    
gTGAACACTTCACGGCGGTTGCTGAGCACAGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGcc                                                                                                                                                  >  4:45971/1‑149 (MQ=255)
 tGACCACTTCACGGCGGTTGCTGCGCACGGTCTTTTCCAGCTTGAAGTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGccc                                                                                                                                                 <  3:306187/149‑1 (MQ=255)
       cTTCACGGCGGTTGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCccgccg                                                                                                                                             <  1:241211/147‑1 (MQ=255)
       cTTCACGGCGGTTGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCccgccg                                                                                                                                             <  1:246501/147‑1 (MQ=255)
       cTTCACGGCGGTTGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCccgccg                                                                                                                                             >  2:241211/1‑147 (MQ=255)
       cTTCACGGCGGTTGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCccgccg                                                                                                                                             >  2:246501/1‑147 (MQ=255)
        ttCACGGCGGTTGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCgccgggc                                                                                                                                          >  3:261795/1‑149 (MQ=255)
        ttCACGGCGGTTGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCgccgggc                                                                                                                                          >  4:272960/1‑149 (MQ=255)
           aCGGCGGTTGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCgccgggccgg                                                                                                                                       <  6:301045/149‑1 (MQ=255)
            cggcggTTGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGCTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGgc                                                                                                                                      >  4:41539/1‑149 (MQ=255)
                 gTTGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCga                                                                                                                                 <  7:96696/149‑1 (MQ=255)
                  ttGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCCGGCCCGGCGGGCCTGGGCTGCGCCgac                                                                                                                                <  3:74118/149‑2 (MQ=38)
                     cTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCAGCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCgatgat                                                                                                                             >  5:261633/1‑149 (MQ=255)
                     cTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCgatgat                                                                                                                             <  3:334896/149‑1 (MQ=255)
                      tGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGatgatg                                                                                                                            >  3:212042/1‑149 (MQ=255)
                      tGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGatgatg                                                                                                                            >  3:361573/1‑149 (MQ=255)
                        aGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATgg                                                                                                                           <  7:179688/148‑1 (MQ=255)
                            cGGTC‑TTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAAcgc                                                                                                                     >  1:363458/1‑149 (MQ=255)
                             ggTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAAcgc                                                                                                                     >  5:180408/1‑149 (MQ=255)
                             ggTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAAcgc                                                                                                                     >  6:63221/1‑149 (MQ=255)
                             ggTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACATCGGCGCCGGCCCGGCGGGCCTGGGCTGCGCCgacg                                                                                                                               >  5:109388/1‑137 (MQ=37)
                             ggTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACATCGGCGCCGGCCCGGCGGGCCTGGGCTGCGCCgacg                                                                                                                               <  6:109388/139‑3 (MQ=37)
                              gTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCccgc                                                                                                                                               <  7:53335/122‑1 (MQ=255)
                               tCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACgcgc                                                                                                                   >  4:20521/1‑149 (MQ=255)
                                cTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCt                                                                                                                  <  3:272960/149‑1 (MQ=255)
                                 ttttCCAGCTTGAACTCGGGGATATCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCtt                                                                                                                 >  5:305496/1‑149 (MQ=255)
                                   ttCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCtt                                                                                                                 <  2:61776/147‑1 (MQ=255)
                                      cAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCgg                                                                                                            >  8:166033/1‑149 (MQ=255)
                                              aCTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTg                                                                                                    <  3:45971/149‑1 (MQ=255)
                                               cTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGa                                                                                                   <  3:20521/149‑1 (MQ=255)
                                                tCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGAc                                                                                                  >  5:211947/1‑149 (MQ=255)
                                                  ggggATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGAc                                                                                                  <  7:39058/147‑1 (MQ=255)
                                                   gggCTACCGAAGGGCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGGCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCtt                                                                                               <  5:374290/149‑1 (MQ=255)
                                                   gggATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCGAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCtt                                                                                               <  2:144993/149‑1 (MQ=255)
                                                    ggATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAAc                                                                                                                       <  4:123213/124‑1 (MQ=255)
                                                    ggATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAAc                                                                                                                       >  3:123213/1‑124 (MQ=255)
                                                    ggATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTg                                                                                              <  5:270595/149‑1 (MQ=255)
                                                     gATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTgg                                                                                             <  4:261795/149‑1 (MQ=255)
                                                          cGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATg                                                                                        >  1:277135/1‑149 (MQ=255)
                                                          cGAAGGTCAGCAGGCCACCGATATCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCTGCTCCGATGATGGCAACGCGCTTGCCCGTTGGCTTGACCTTGGACATg                                                                                        >  5:154086/1‑149 (MQ=255)
                                                             aGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTcc                                                                                     <  3:58674/149‑1 (MQ=255)
                                                               gTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCgg                                                                                   <  6:261633/149‑1 (MQ=255)
                                                                       gCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGc                                                                           >  6:87193/1‑149 (MQ=255)
                                                                                 tcAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCg                                                                   >  3:367139/1‑147 (MQ=255)
                                                                                   aGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAgg                                                               >  3:84992/1‑149 (MQ=255)
                                                                                   aGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAgg                                                               >  3:107/1‑149 (MQ=255)
                                                                                   aGGGTGTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAg                                                                <  4:53002/148‑1 (MQ=255)
                                                                                     ggTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCg                                                             <  4:367139/149‑1 (MQ=255)
                                                                                               gAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTg                                                    <  4:212042/148‑1 (MQ=255)
                                                                                               gAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGa                                                   >  7:286083/1‑149 (MQ=255)
                                                                                               gAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGa                                                   <  6:180408/149‑1 (MQ=255)
                                                                                               gAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGa                                                   >  3:216498/1‑149 (MQ=255)
                                                                                               gAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGa                                                   <  7:166033/149‑1 (MQ=255)
                                                                                                 acacCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATg                                                 >  5:11882/1‑149 (MQ=255)
                                                                                                   accaccGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTg                                                    <  4:47729/144‑1 (MQ=255)
                                                                                                   accaccGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGaagat                                               >  3:47729/1‑145 (MQ=255)
                                                                                                    ccaccGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTAc                                              <  2:363458/149‑1 (MQ=255)
                                                                                                    ccaccGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTAc                                              <  6:335191/149‑1 (MQ=255)
                                                                                                    ccaccGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTAc                                              <  4:216498/149‑1 (MQ=255)
                                                                                                     caccGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGCTGGCAACGCGCTTGCCGGTTGGCTGGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACt                                             >  5:357262/1‑149 (MQ=255)
                                                                                                     caccGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAAGGCGCTTGCCGGTTGGCTTGACCTTGGACTTGTCCGGACGCCAGCCCATGGCGAAGGCGGAGTCGGTGATGTACt                                             <  1:268701/149‑1 (MQ=255)
                                                                                                     caccGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACt                                             >  4:302468/1‑149 (MQ=255)
                                                                                                     caccGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACt                                             >  3:341343/1‑149 (MQ=255)
                                                                                                      accGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACtt                                            <  3:322090/149‑1 (MQ=255)
                                                                                                      accGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACtt                                            <  4:322892/149‑1 (MQ=255)
                                                                                                      accGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACtt                                            >  4:322090/1‑149 (MQ=255)
                                                                                                           ggTAACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGAt                                                  <  5:268631/138‑1 (MQ=255)
                                                                                                           ggTAACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGAt                                                  >  6:268631/1‑138 (MQ=255)
                                                                                                                 cccGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGc                                 >  1:1869/1‑149 (MQ=255)
                                                                                                                 cccGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGc                                 <  5:63221/149‑1 (MQ=255)
                                                                                                                  ccGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGcc                                >  7:149398/1‑149 (MQ=255)
                                                                                                                   cGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCAGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGcc                                >  6:215695/1‑148 (MQ=255)
                                                                                                                     gcgcgcACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGAt                             <  2:277135/149‑1 (MQ=255)
                                                                                                                        cgcACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGt                          <  4:84992/149‑1 (MQ=255)
                                                                                                                             cAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACcg                     >  1:80424/1‑149 (MQ=255)
                                                                                                                             cAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACcg                     >  2:90620/1‑149 (MQ=255)
                                                                                                                               gCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACcgcg                   >  8:5219/1‑149 (MQ=255)
                                                                                                                                 tcatCGGCGCCGGCCCGGCGGGCCTGGGCTGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGAACTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACCGCGcc                 >  5:266868/4‑149 (MQ=38)
                                                                                                                                 aCGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCAcc                      >  1:218328/1‑144 (MQ=255)
                                                                                                                                 aCGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCAcc                      <  2:218328/144‑1 (MQ=255)
                                                                                                                                 aCGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACCGCGcc                 <  8:286083/149‑1 (MQ=255)
                                                                                                                                   gTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACCGCGCCg                >  7:293836/1‑148 (MQ=255)
                                                                                                                                     cGGCGCCGGCCCGGCGGGCCTGGGCTGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACCGCGCCGaaa             >  5:16446/1‑149 (MQ=255)
                                                                                                                                      ggCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACCGCGCCGAAAc            >  1:143450/1‑149 (MQ=255)
                                                                                                                                       gggcAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACCGCGCCGAAAcc           <  6:226503/147‑1 (MQ=255)
                                                                                                                                       gcgcAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACCGCGCCGAAAcc           <  3:41539/149‑1 (MQ=255)
                                                                                                                                             cccAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACCGCGCCGAAACCGTCGtt     <  3:302468/149‑1 (MQ=255)
                                                                                                                                             cccAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACCGCGCCGAAACCGTCGtt     >  6:73335/1‑149 (MQ=255)
                                                                                                                                               cAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACCGCGCCGAAACCGTCGtt     >  7:295958/1‑147 (MQ=255)
                                                                                                                                               cAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACCGCGCCGAAACCGTCGtt     >  4:311476/1‑147 (MQ=255)
                                                                                                                                               cAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACCGCGCCGAAACCGTCGtt     >  4:316829/1‑147 (MQ=255)
                                                                                                                                                aGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACCGCGCCGAAACCGTCGTTCAg  <  5:73335/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
GTGAACACTTCACGGCGGTTGCTGAGCACGGTCTTTTCCAGCTTGAACTCGGGGATACCGAAGGTCAGCAGGCCACCGATCTCAGGGTTTTTGTCGAACACCACCGGGGTTACCCCGGCGCGCACCAGCACGTCGGCGCAGCCCAGGCCCGCCGGGCCGGCGCCGATGATGGCAACGCGCTTGCCGGTTGGCTTGACCTTGGACATGTCCGGACGCCAGCCCATGGCGAAGGCGGTGTCGGTGATGTACTTCTCCACCGAGCCGATGGTCACCGCGCCGAAACCGTCGTTCAG  >  NC_002947/5792702‑5792994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑