Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,813,216	A→T	5.5%	N46I (AAC→ATC)	argS →	arginine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,813,216	0	A	T	5.5%	222.4 / 2.7	72	N46I (AAC→ATC)	argS	arginine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (38/30);  new base T (2/2);  total (40/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.59e-01

CCATGAAAGACACCATTCGCCAGCTGATCCAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAGAACACCGA  >  NC_002947/5813078‑5813360                                                                                                                                           |                                                                                                                                                 ccATGAAAGACACCATTCGCCAGCTGATCCAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCatg                                                                                                                                        <  8:231222/149‑1 (MQ=255)  cATGAAAGACACCATTCGCCAGCTGATCCAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCatga                                                                                                                                       >  3:171520/1‑149 (MQ=255)   aTGAAAGACACCATTCGCCAGCTGATCCAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCatgat                                                                                                                                      >  6:64503/1‑149 (MQ=255)     gAAAGACACCATTCGCCAGCTGATCCAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGc                                                                                                                                    >  5:199924/1‑149 (MQ=255)        aGACACCATTCGCCAGCTGATCCAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCATCATGGCGATGTTGCTgg                                                                                                                                 <  2:324504/149‑1 (MQ=255)         gACACCATTCGCCAGCTGATCCAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCATCATGGCGATGTTGCTGGc                                                                                                                                <  2:193017/149‑1 (MQ=255)          acacCATTCGCCAGCTGATCCAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCaa                                                                                                                                                  >  4:264013/1‑130 (MQ=255)          acacCATTCGCCAGCTGATCCAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCaa                                                                                                                                                  <  3:264013/130‑1 (MQ=255)            acCATTCGCCAGCTGATCCAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGcc                                                                                                                               >  7:307412/1‑147 (MQ=255)                 tCGCCAGCTGATCCAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAgccgg                                                                                                                        >  5:123020/1‑149 (MQ=255)                    ccAGCTGATCCAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAgccggccg                                                                                                                     <  6:199924/149‑1 (MQ=255)                      aGCTGATCCAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCcggccggc                                                                                                                   <  4:357219/149‑1 (MQ=255)                        cTGATCCAGCAAGCCCTCGCCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCAt                                                                                                                 <  2:87177/149‑1 (MQ=255)                              cAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGcc                                                                                                           >  8:8179/1‑149 (MQ=255)                                gCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCcgc                                                                                                         >  1:264794/1‑149 (MQ=255)                                      ccTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGCCCCACGGCGACTTCGCCAGCAACCTCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGAcc                                                                                                   >  2:344161/1‑149 (MQ=255)                                       cTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCt                                                                                                  >  1:207366/1‑149 (MQ=255)                                          aCCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCa                                                                                                                  >  2:364533/1‑130 (MQ=255)                                          aCCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCa                                                                                                                  <  1:364533/130‑1 (MQ=255)                                             cAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCAt                                                                                                                 >  8:47870/1‑128 (MQ=255)                                             cAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCAt                                                                                                                 <  7:47870/128‑1 (MQ=255)                                              aaCTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGa                                                                                            >  1:338715/1‑148 (MQ=255)                                              aaCTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCAACGGAGACTTCGCCAGCAACATCGCCATGATTCTGGCCAAGCCGGCCGGCATGACGACGCGCGACCAcgacga                                                                                            >  7:312322/1‑148 (MQ=255)                                               aCTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGaa                                                                                           >  2:214463/1‑148 (MQ=255)                                                 tCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGaaaaa                                                                                        >  8:314298/1‑149 (MQ=255)                                                   gTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGaaaaa                                                                                        <  2:264794/147‑1 (MQ=255)                                                      aCCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGAt                                                                                   <  5:370427/149‑1 (MQ=255)                                                      aCCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGAt                                                                                   <  1:214463/149‑1 (MQ=255)                                                      aCCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGAt                                                                                   <  2:338715/149‑1 (MQ=255)                                                          aCGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAAc                                                                               <  7:271634/149‑1 (MQ=255)                                                                        gAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAg                                                                 >  5:39071/1‑149 (MQ=255)                                                                         aaGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCa                                                                  <  5:64503/147‑1 (MQ=255)                                                                           gggCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAgcgc                                                              >  4:286310/1‑149 (MQ=255)                                                                               tGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGa                                                           >  1:257435/1‑148 (MQ=255)                                                                                    ccGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAg                                                     >  7:149273/1‑149 (MQ=255)                                                                                        cGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAg                                                 >  8:341780/1‑149 (MQ=255)                                                                                         gATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAgg                                                >  3:54076/1‑149 (MQ=255)                                                                                             cAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCga                                            <  8:307412/149‑1 (MQ=255)                                                                                              aGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTc                                              >  1:203602/1‑146 (MQ=255)                                                                                                gTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCa                                                   >  2:70720/1‑139 (MQ=255)                                                                                                gTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCa                                                   <  1:70720/139‑1 (MQ=255)                                                                                                 tGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGAtt                                        >  2:68053/1‑149 (MQ=255)                                                                                                    aaaaCGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTg                                       >  4:4666/1‑147 (MQ=255)                                                                                                        cGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTg                                       <  5:116427/143‑1 (MQ=255)                                                                                                        cGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTg                                       >  6:116427/1‑143 (MQ=255)                                                                                                          cccGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGcccc                               <  6:39071/149‑1 (MQ=255)                                                                                                           ccGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCg                              >  3:336290/1‑149 (MQ=255)                                                                                                             gggACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGc                                      <  4:290563/139‑1 (MQ=255)                                                                                                             gggACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGc                                      >  3:290563/1‑139 (MQ=255)                                                                                                             gggACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGc                            <  3:286310/149‑1 (MQ=255)                                                                                                                aCAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTc                         <  8:149273/149‑1 (MQ=255)                                                                                                                 cAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTc                         <  2:203602/148‑1 (MQ=255)                                                                                                                    gACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTc                      >  2:19506/1‑148 (MQ=255)                                                                                                                       ccACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAAct                  >  4:70257/1‑149 (MQ=255)                                                                                                                         aCGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGCCATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAActtc                >  7:324218/1‑149 (MQ=255)                                                                                                                         aCGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAActtc                >  3:91019/1‑149 (MQ=255)                                                                                                                          cGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCCGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCCActtct               >  8:174086/1‑149 (MQ=255)                                                                                                                           ggCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAActtctt              >  7:20449/1‑149 (MQ=255)                                                                                                                            gCGACTTCGCCAGCAACATCTCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTTATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACttcttc             <  8:312322/149‑1 (MQ=255)                                                                                                                            gCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACttcttc             <  4:91019/149‑1 (MQ=255)                                                                                                                            gCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACttcttc             <  4:171520/149‑1 (MQ=255)                                                                                                                              gACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCa           <  7:314298/149‑1 (MQ=255)                                                                                                                              gACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCa           <  2:207366/149‑1 (MQ=255)                                                                                                                              gACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCa           <  4:54076/149‑1 (MQ=255)                                                                                                                               aCTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAg          <  7:8179/149‑1 (MQ=255)                                                                                                                                cTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAg          >  3:115053/1‑148 (MQ=255)                                                                                                                                   cGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAGaa        <  7:341780/147‑1 (MQ=255)                                                                                                                                    gCCAGCATCATGGCGATGTTGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAGAacac     >  5:223678/1‑149 (MQ=255)                                                                                                                                    gCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTcc            <  7:336181/142‑1 (MQ=255)                                                                                                                                    gCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTcc            >  8:336181/1‑142 (MQ=255)                                                                                                                                      cAGCATCATGGCGATGTTGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAGAACACCg   >  7:301206/1‑149 (MQ=255)                                                                                                                                      cAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAGAACAcc    >  6:79455/1‑148 (MQ=255)                                                                                                                                      cAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAGAACACCg   <  6:123020/149‑1 (MQ=255)                                                                                                                                       aGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCTACGGCGACGAAAAACTGATAAACGCACTGCCGGCCAGCGCCGGCATCAGCAAGGGCGAGAGTGCAGGCCAAGGCTTCCTCCAGTGCTTCCAGAACACCgc  >  6:253252/1‑148 (MQ=255)                                                                                                                                           |                                                                                                                                                 CCATGAAAGACACCATTCGCCAGCTGATCCAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAGAACACCGA  >  NC_002947/5813078‑5813360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑