Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,813,227	A→T	5.5%	M50L (ATG→TTG)	argS →	arginine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,813,227	0	A	T	5.5%	226.7 / 2.7	72	M50L (ATG→TTG)	argS	arginine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (37/31);  new base T (2/2);  total (39/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.89e-01

CATGAAAGACACCATTCGCCAGCTGATCCAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAGAACACCGACGCCCTGG  >  NC_002947/5813079‑5813368                                                                                                                                                     |                                                                                                                                              cATGAAAGACACCATTCGCCAGCTGATCCAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCatga                                                                                                                                               >  3:171520/1‑149 (MQ=255)  aTGAAAGACACCATTCGCCAGCTGATCCAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCatgat                                                                                                                                              >  6:64503/1‑149 (MQ=255)    gAAAGACACCATTCGCCAGCTGATCCAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGc                                                                                                                                            >  5:199924/1‑149 (MQ=255)       aGACACCATTCGCCAGCTGATCCAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCATCATGGCGATGTTGCTgg                                                                                                                                         <  2:324504/149‑1 (MQ=255)        gACACCATTCGCCAGCTGATCCAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCATCATGGCGATGTTGCTGGc                                                                                                                                        <  2:193017/149‑1 (MQ=255)           acCATTCGCCAGCTGATCCAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGcc                                                                                                                                       >  7:307412/1‑147 (MQ=255)                tCGCCAGCTGATCCAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAgccgg                                                                                                                                >  5:123020/1‑149 (MQ=255)                   ccAGCTGATCCAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAgccggccg                                                                                                                             <  6:199924/149‑1 (MQ=255)                     aGCTGATCCAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCcggccggc                                                                                                                           <  4:357219/149‑1 (MQ=255)                       cTGATCCAGCAAGCCCTCGCCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCAt                                                                                                                         <  2:87177/149‑1 (MQ=255)                             cAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGcc                                                                                                                   >  8:8179/1‑149 (MQ=255)                               gCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCcgc                                                                                                                 >  1:264794/1‑149 (MQ=255)                                     ccTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGCCCCACGGCGACTTCGCCAGCAACCTCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGAcc                                                                                                           >  2:344161/1‑149 (MQ=255)                                      cTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCt                                                                                                          >  1:207366/1‑149 (MQ=255)                                         aCCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCa                                                                                                                          >  2:364533/1‑130 (MQ=255)                                         aCCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCa                                                                                                                          <  1:364533/130‑1 (MQ=255)                                            cAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCAt                                                                                                                         >  8:47870/1‑128 (MQ=255)                                            cAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCAt                                                                                                                         <  7:47870/128‑1 (MQ=255)                                             aaCTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGa                                                                                                    >  1:338715/1‑148 (MQ=255)                                             aaCTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCAACGGAGACTTCGCCAGCAACATCGCCATGATTCTGGCCAAGCCGGCCGGCATGACGACGCGCGACCAcgacga                                                                                                    >  7:312322/1‑148 (MQ=255)                                              aCTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGaa                                                                                                   >  2:214463/1‑148 (MQ=255)                                                tCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGaaaaa                                                                                                >  8:314298/1‑149 (MQ=255)                                                  gTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGaaaaa                                                                                                <  2:264794/147‑1 (MQ=255)                                                     aCCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGAt                                                                                           <  2:338715/149‑1 (MQ=255)                                                     aCCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGAt                                                                                           <  1:214463/149‑1 (MQ=255)                                                     aCCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGAt                                                                                           <  5:370427/149‑1 (MQ=255)                                                         aCGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAAc                                                                                       <  7:271634/149‑1 (MQ=255)                                                                       gAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAg                                                                         >  5:39071/1‑149 (MQ=255)                                                                        aaGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCa                                                                          <  5:64503/147‑1 (MQ=255)                                                                          gggCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAgcgc                                                                      >  4:286310/1‑149 (MQ=255)                                                                              tGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGa                                                                   >  1:257435/1‑148 (MQ=255)                                                                                   ccGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAg                                                             >  7:149273/1‑149 (MQ=255)                                                                                       cGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAg                                                         >  8:341780/1‑149 (MQ=255)                                                                                        gATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAgg                                                        >  3:54076/1‑149 (MQ=255)                                                                                            cAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCga                                                    <  8:307412/149‑1 (MQ=255)                                                                                             aGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTc                                                      >  1:203602/1‑146 (MQ=255)                                                                                               gTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCa                                                           <  1:70720/139‑1 (MQ=255)                                                                                               gTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCa                                                           >  2:70720/1‑139 (MQ=255)                                                                                                tGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGAtt                                                >  2:68053/1‑149 (MQ=255)                                                                                                   aaaaCGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTg                                               >  4:4666/1‑147 (MQ=255)                                                                                                       cGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTg                                               <  5:116427/143‑1 (MQ=255)                                                                                                       cGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTg                                               >  6:116427/1‑143 (MQ=255)                                                                                                         cccGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGcccc                                       <  6:39071/149‑1 (MQ=255)                                                                                                          ccGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCg                                      >  3:336290/1‑149 (MQ=255)                                                                                                            gggACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGc                                              <  4:290563/139‑1 (MQ=255)                                                                                                            gggACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGc                                              >  3:290563/1‑139 (MQ=255)                                                                                                            gggACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGc                                    <  3:286310/149‑1 (MQ=255)                                                                                                               aCAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTc                                 <  8:149273/149‑1 (MQ=255)                                                                                                                cAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTc                                 <  2:203602/148‑1 (MQ=255)                                                                                                                   gACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTc                              >  2:19506/1‑148 (MQ=255)                                                                                                                      ccACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAAct                          >  4:70257/1‑149 (MQ=255)                                                                                                                        aCGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGCCATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAActtc                        >  7:324218/1‑149 (MQ=255)                                                                                                                        aCGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAActtc                        >  3:91019/1‑149 (MQ=255)                                                                                                                         cGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCCGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCCActtct                       >  8:174086/1‑149 (MQ=255)                                                                                                                          ggCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAActtctt                      >  7:20449/1‑149 (MQ=255)                                                                                                                           gCGACTTCGCCAGCAACATCTCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTTATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACttcttc                     <  8:312322/149‑1 (MQ=255)                                                                                                                           gCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACttcttc                     <  4:171520/149‑1 (MQ=255)                                                                                                                           gCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACttcttc                     <  4:91019/149‑1 (MQ=255)                                                                                                                             gACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCa                   <  7:314298/149‑1 (MQ=255)                                                                                                                             gACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCa                   <  4:54076/149‑1 (MQ=255)                                                                                                                             gACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCa                   <  2:207366/149‑1 (MQ=255)                                                                                                                              aCTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAg                  <  7:8179/149‑1 (MQ=255)                                                                                                                               cTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAg                  >  3:115053/1‑148 (MQ=255)                                                                                                                                  cGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAGaa                <  7:341780/147‑1 (MQ=255)                                                                                                                                   gCCAGCATCATGGCGATGTTGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAGAacac             >  5:223678/1‑149 (MQ=255)                                                                                                                                   gCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTcc                    <  7:336181/142‑1 (MQ=255)                                                                                                                                   gCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTcc                    >  8:336181/1‑142 (MQ=255)                                                                                                                                     cAGCATCATGGCGATGTTGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAGAACACCg           >  7:301206/1‑149 (MQ=255)                                                                                                                                     cAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAGAACAcc            >  6:79455/1‑148 (MQ=255)                                                                                                                                     cAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAGAACACCg           <  6:123020/149‑1 (MQ=255)                                                                                                                                      aGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCTACGGCGACGAAAAACTGATAAACGCACTGCCGGCCAGCGCCGGCATCAGCAAGGGCGAGAGTGCAGGCCAAGGCTTCCTCCAGTGCTTCCAGAACACCgc          >  6:253252/1‑148 (MQ=255)                                                                                                                                            aTCGACATTATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCACTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAGAACACCGACGCCCt    <  3:348762/149‑1 (MQ=255)                                                                                                                                              cGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAGAACACCGACGCCCTgg  >  3:365994/1‑149 (MQ=255)                                                                                                                                               gCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCACGGCTTCCTCAACTTCTTCCAGAACACCGACGCCCTgg  <  8:20449/148‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                              CATGAAAGACACCATTCGCCAGCTGATCCAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAGAACACCGACGCCCTGG  >  NC_002947/5813079‑5813368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑