Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,856,783 C→A 5.1% P237H (CCC→CAC)  PP_5133 → membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,856,7830CA 5.1% 256.9 / 3.2 78P237H (CCC→CAC) PP_5133membrane protein
Reads supporting (aligned to +/- strand):  ref base C (42/32);  new base A (2/2);  total (44/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.37e-01

TGCTCACCGCGCTGGGCATGCTACTGGTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCT  >  NC_002947/5856636‑5856929
                                                                                                                                                    |                                                                                                                                                  
tGCTCACCGCGCTGGGCATGCTACTGGTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGcc                                                                                                                                                    <  4:234973/148‑1 (MQ=255)
     aCCGCGCTGGGCATGCTACTGGTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCcgacg                                                                                                                                               >  3:48948/1‑148 (MQ=255)
      ccGCGCTGGGCATGCTACTGGTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGaa                                                                                                                                             <  1:159309/149‑1 (MQ=255)
       cgcgCTGGGCATGCTACTGGTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAg                                                                                                                                            <  5:313271/149‑1 (MQ=255)
            tGGGCATGCTACTGGTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCTCCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGTCGGGCACGGCGAAGcccag                                                                                                                                       <  3:30134/149‑4 (MQ=255)
              ggCATGCTACTGGTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGTCGGGCACGGCGAAGcccagca                                                                                                                                     <  2:120821/149‑6 (MQ=255)
                  tGCTACTGGTCTTGCTGGCGCCCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCcgc                                                                                                                                 <  4:174576/149‑1 (MQ=255)
                  tGCTACTGGTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCcgc                                                                                                                                 <  6:68204/149‑1 (MQ=255)
                        tGGTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAgc                                                                                                                           >  5:40477/1‑149 (MQ=255)
                         ggTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAgcg                                                                                                                          >  8:183639/1‑149 (MQ=255)
                          gTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGCCCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGAGGAAGCCATGATCCGCGCCAgcgg                                                                                                                         >  8:308725/1‑149 (MQ=255)
                           tCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAgcggc                                                                                                                        <  1:27607/149‑1 (MQ=255)
                             ttGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TTCCGTCGTTGCTGGTCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCTGCGa                                                                                                                      >  8:196510/1‑149 (MQ=255)
                             ttGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGa                                                                                                                      >  8:159313/1‑149 (MQ=255)
                               gCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGa                                                                                                                      <  2:334027/147‑1 (MQ=255)
                                 tGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGc                                                                                                                  >  7:96830/1‑149 (MQ=255)
                                 tGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGc                                                                                                                  >  1:139869/1‑149 (MQ=255)
                                 tGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGc                                                                                                                  >  6:99269/1‑149 (MQ=255)
                                  ggCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGcc                                                                                                                 >  8:25330/1‑149 (MQ=255)
                                   gCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCt                                                                                                                >  3:155748/1‑149 (MQ=255)
                                     gACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGc                                                                                                              <  8:96830/149‑1 (MQ=255)
                                          ggCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGcc                                                                                                         >  2:61394/1‑149 (MQ=255)
                                          ggCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGcc                                                                                                         <  5:120725/149‑1 (MQ=255)
                                             gcTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTg                                                                                                      <  2:139869/149‑1 (MQ=255)
                                               tATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGa                                                                                                    >  6:177056/1‑149 (MQ=255)
                                               tATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGa                                                                                                    >  5:270610/1‑149 (MQ=255)
                                                 tGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGAc                                                                                                   <  7:198696/148‑1 (MQ=255)
                                                   gCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTg                                                                                                <  7:131927/149‑1 (MQ=255)
                                                     tttGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGc                                                                                              >  3:132896/1‑149 (MQ=255)
                                                         tCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TCCCGGCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGc                                                                                          <  6:290596/149‑1 (MQ=255)
                                                           tGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGc                                                                                          >  7:188647/1‑147 (MQ=255)
                                                           tGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCa                                                                                        <  4:155748/149‑1 (MQ=255)
                                                           tGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAACACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGc                                                                                          >  1:87944/1‑147 (MQ=255)
                                                            gggAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAg                                                                                       >  8:61570/1‑149 (MQ=255)
                                                             ggAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAgg                                                                                      >  6:341307/1‑149 (MQ=255)
                                                             ggAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAg                                                                                       <  1:42709/148‑1 (MQ=255)
                                                             ggAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAg                                                                                       <  7:25330/148‑1 (MQ=255)
                                                              gAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGc                                                                                     <  8:188647/149‑1 (MQ=255)
                                                                   caccTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCAACCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTgg                                                                                 >  1:98394/1‑149 (MQ=255)
                                                                   caccTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTggg                                                                                >  3:5844/1‑149 (MQ=255)
                                                                   caccTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTggg                                                                                <  4:48948/149‑1 (MQ=255)
                                                                   caccTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTCCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTggg                                                                                >  4:15037/1‑149 (MQ=255)
                                                                    accTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGc                                                                               >  7:340446/1‑149 (MQ=255)
                                                                      cTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGc                                                                               >  6:101164/1‑147 (MQ=255)
                                                                           tCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAgctgg                                                                        >  7:301651/1‑149 (MQ=255)
                                                                                 ccgACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGCCGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTc                                                                  <  5:177056/149‑1 (MQ=255)
                                                                                       cGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCgtg                                                            <  7:183639/149‑1 (MQ=255)
                                                                                         ttcattcaCCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCgtggt                                                          >  6:222396/1‑149 (MQ=255)
                                                                                            attcaCCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGAGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGc                                                        <  6:291309/148‑1 (MQ=255)
                                                                                                  cTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCACCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGtata                                                 >  7:30033/1‑149 (MQ=255)
                                                                                                       ccAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGcc                                            >  7:90473/1‑149 (MQ=255)
                                                                                                       ccAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGcc                                            >  7:306716/1‑149 (MQ=255)
                                                                                                        cAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCct                                           >  5:195727/1‑149 (MQ=255)
                                                                                                        cAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCct                                           <  4:5844/149‑1 (MQ=255)
                                                                                                        cAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCct                                           >  8:87255/1‑149 (MQ=255)
                                                                                                        cAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCct                                           <  8:90473/149‑1 (MQ=255)
                                                                                                            cACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCctgctg                                       >  8:104952/1‑149 (MQ=255)
                                                                                                              cTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGcc                                     <  4:353708/149‑1 (MQ=255)
                                                                                                                 ggCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCg                                  >  4:128182/1‑149 (MQ=255)
                                                                                                                    gcCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCct                               <  6:270610/149‑1 (MQ=255)
                                                                                                                      cc‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCctgc                             >  7:119133/1‑149 (MQ=255)
                                                                                                                       c‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCgtg                                                            >  3:200839/1‑117 (MQ=255)
                                                                                                                       c‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCgtg                                                            <  4:200839/117‑1 (MQ=255)
                                                                                                                         tCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCAGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCctgctg                           >  3:201230/1‑149 (MQ=255)
                                                                                                                           ccGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCctgctgc                          <  7:87255/148‑1 (MQ=255)
                                                                                                                             gtcgtTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTgg                       >  7:13088/1‑149 (MQ=255)
                                                                                                                              tcgtTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGc                      <  4:132896/149‑1 (MQ=255)
                                                                                                                                gtTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCg                    >  5:176266/1‑149 (MQ=255)
                                                                                                                                gtTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCg                    <  2:98394/149‑1 (MQ=255)
                                                                                                                                 ttGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCgg                   <  5:341307/149‑1 (MQ=255)
                                                                                                                                    cTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCGGGt                 >  3:96274/1‑148 (MQ=255)
                                                                                                                                     tGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCGGGTTa               >  7:96536/1‑149 (MQ=255)
                                                                                                                                       ggCTTCGTCGGGCACGGCGAAGCCCTGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCGGGTTATg             >  5:90574/1‑149 (MQ=255)
                                                                                                                                         cTTCGTCGGGCACGGCGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCGGGTTATGcc           >  7:72643/1‑149 (MQ=255)
                                                                                                                                          ttCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCGGGTTATGCCt          >  3:354385/1‑149 (MQ=255)
                                                                                                                                            cGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCGGGTTATGCCt          <  8:119133/147‑1 (MQ=255)
                                                                                                                                             gCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCGGGTTATGCCtgtg       >  4:253910/1‑149 (MQ=255)
                                                                                                                                             gCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCGGGTTATGCCtgtg       <  2:87944/149‑1 (MQ=255)
                                                                                                                                                  gCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCGGGTTATGCCTGtggcgct  <  7:212487/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TGCTCACCGCGCTGGGCATGCTACTGGTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCC‑TCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCT  >  NC_002947/5856636‑5856929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑