Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,861,063	G→C	6.8%	F222L (TTC→TTG)	PP_5136 ←	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,861,063	0	G	C	6.8%	177.7 / 3.7	59	F222L (TTC→TTG)	PP_5136	ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (26/29);  new base C (2/2);  total (28/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.59e-01

GCCGGCAACAGCAGCACCATGCCGATCAGCGCGCCACGGCCGAACTGTTGCTGGCCCACCACCGCCTTGTAGGCTTCCAGCGCCAGCACTTGGTAGTCGCCCCCCACCACGACCGGTACACCGAAGTCTGTGATAGTCAGCGTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAGGCCGGCCAGGTGATGCTGGTGAACGCACGCCACGAGCCTGCCCCCATGCTGGAGGCCGCATCGAACAGGCGAGCATCGGCCAGTGACAGTGCCGACAGCAGAATCATC  >  NC_002947/5860920‑5861210                                                                                                                                                |                                                                                                                                                    gCCGGCAACAGCAGCACCATGCCGATCAGCGCGCCACGGCCGAACTGTTGCTGGCCCACCACCGCCTTGTAGGCTTCCAGCGCCAGCACTTGGTAGTCGCCCCCCACCACGACCGGTACACCGAAGTCTGTGATAGTCAGCGTGAATAc                                                                                                                                                >  7:307059/1‑149 (MQ=255)   cGGCAACAGCAGCACCATGCCGATCAGCGCGCCACGGCCGGACTGTTGCTGGCCCACCACCGCCTTGTATGCTTCCAGCGCCAGCACTTGGTAGTCGCCCCCCACCACGACCGGTACACCGAAGTCTGTGATAGTCAGCGTGAATACCa                                                                                                                                              <  3:333992/149‑1 (MQ=255)    ggCAACAGCAGCACCATGCCGATCAGCGCGCCACGGCCGAACTGTTGCTGGCCCACCACCGCCTTGTAGGCTTCCAGCGCCAGCACTTGGTAGTCGCCCCCCACCACGACCGGTACACCGAAGTCTGTGATAGTCAGCGTGAATACCAg                                                                                                                                             <  3:222532/149‑1 (MQ=255)    ggCAACAGCAGCACCATGCCGATCAGCGCGCCACGGCCGAACTGTTGCTGGCCCACCACCGCCTTGTAGGCTTCCAGCGCCAGCACTTGGTAGTCGCCCCCCACCACGACCGGTACACCGAAGTCTGTGATAGTCAGCGTGAATACCAg                                                                                                                                             <  6:146849/149‑1 (MQ=255)       aaCAGCAGCACCATGCCGATCAGCGCGCCACGGCCGAACTGTTGCTGGCCCACCACCGCCTTGTAGGCTTCCAGCGCCAGCACTTGGTAGTCGCCCCCCACCACGACCGGTACACAGAAGTCTGTGATAGTCAGCGTGAATACCAGGc                                                                                                                                           >  3:151266/1‑148 (MQ=255)            cagcaCCATGCCGATCAGCGCGCCACGGCCGAACTGTTGCTGGCCCACCACCGCCTTGTAGGCTTCCAGCGCCAGCACTTGGTAGTCGCCCCCCACCACGACCGGTACACCGAAGTCTGTGATAGTCAGCGTGAATACCAGGCAAAAcg                                                                                                                                     <  8:127296/149‑1 (MQ=255)                   aTGCCGATCAGCGCGCCACGGCCGAACTGTTGCTGGCCCACCACCGCCTTGTAGGCTTCCAGCGCCAGCACTTGGTAGTCGCCCCCCACCACGACCGGTACACCGAAGTCTGTGATAGTCAGCGTGAATACCAGGCAAAACGCCGCGaa                                                                                                                              >  7:195439/1‑149 (MQ=255)                     gCCGATCAGCGCGCCACGGCCGAACTGTTGCTGGCCCACCACCGCCTTGTAGGCTTCCAGCGCCAGCACTTGGTAGTCGCCCCCCACCACGACCGGTACACCGAAGTCTGTGATAGTCAGCGTGAATACCAGGCAAAACGCCGCGAATa                                                                                                                            >  8:69578/1‑149 (MQ=255)                        gATCAGCGCGCCACGGCCGAACTGTTGCTGGCCCACCACCGCCTTGTAGGCTTCCAGCGCCAGCACTTGGTAGTCGCCCCCCACCACGACCGGTACACCGAAGTCTGTGATAGTCAGCGTCAATACCAGGCAAAACGCCGCGAATACCg                                                                                                                         >  6:279463/1‑149 (MQ=255)                        gATCAGCGCGCCACGGCCGAACTGTTGCTGGCCCACCACCGCCTTGTAGGCTTCCAGCGCCAGCACTTGGTAGTCGCCCCCCACCACGACCGGTACACCGAAGTCTGTGATAGTCAGCGTCAATACCAGGCAAAACGCCGCGAATACCg                                                                                                                         >  6:284565/1‑149 (MQ=255)                                    aCGGCCGAACTGTTGCTGGCCCACCACCGCCTTGTAGGCTTCCAGCGCCAGCACTTGGTAGTCGCCCCCCACCACGACCGGTACACCGAAGTCTGTGATAGTCAGCGTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAgg                                                                                                             <  1:68127/149‑1 (MQ=255)                                       gCCGAACTGTTGCTGGCCCACCACCGCCTTGTAGGCTTCCAGCGCCAGCACTTGGTAGTCGCCCCCCACCACGACCGGTACACCGAAGTCTGTGATAGTCAGCGTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAggccg                                                                                                          <  6:252349/149‑1 (MQ=255)                                         cGAACTGTTGCTGGCCCACCACCGCCTTGTAGGCTTGCAGCGCGAGCACTTGGTAGTCGCCCCCCACCACGACCGGTACAGCGAAGTCTGTGATAGTCAGCGTGAAGACCAGGCAAAACGCCGCGATTACCGCCTGGCGCGcggccggc                                                                                                        >  6:286885/1‑149 (MQ=255)                                         cGAACTGTTGCTGGCCCACCACCGCCTTGTAGGCTTCCAGCGCCAGCACTTGGTAGTCGCCCCCCACCACGACCGGTACACCGAAGTCTGTGATAGTCAGCGTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAggccggc                                                                                                        >  2:355790/1‑149 (MQ=255)                                             cTGTTGCTGGCCCACCACCGCCTTGTAGGCTTCCAGCGCCAGCACTTGGTAGTCGCCCCCCACCACGACCGGTACACCGAAGTCTGTGATAGTCAGCGTCAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAGGCCGGCCAgg                                                                                                    <  5:284565/149‑1 (MQ=255)                                             cTGTTGCTGGCCCACCACCGCCTTGTAGGCTTCCAGCGCCAGCACTTGGTAGTCGCCCCCCACCACGACCGGTACACCGAAGTCTGTGATAGTCAGCGTCAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAGGCCGGCCAgg                                                                                                    <  5:279463/149‑1 (MQ=255)                                                        ccaccaCCGCCTTGTAGGCTTCCAGCGCCAGCACTTGGTAGTCGCCCCCCACCACGACCGGTACACCGAAGTCTGTGATAGTCAGCGTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAGGCCGGCCAGGTGATGCTGGt                                                                                          >  6:240158/1‑148 (MQ=255)                                                           ccaccGCCTTGTAGGCTTCCAGCGCCAGCACTTGGTAGTCGCCCCCCACCACGACCGGTACACCGAAGTCTGTGATAGTCAGCGTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAGGCCGGCCAGGTGATGCTGGTGAac                                                                                      <  3:155364/149‑1 (MQ=255)                                                            caccGCCTTGTAGGCTTCCAGCGCCAGCACTTGGTAGTCGCCCCCCACCACGACCGGTACACCGAAGTCTGTGATAGTCAGCGTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAGGCCGGCCAGGTGATGCTGGTGAacg                                                                                     >  1:300244/1‑149 (MQ=255)                                                                     gTAGGCTTCCAGCGCCAGCACTTGGTAGTCGCCCCCCACCACGACCGGTACACCGAAGTCTGTGATAGTCAGCGTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAGGCCGGCCAGGTGATGCTGGTGAACGCACGcc                                                                               >  2:231783/1‑146 (MQ=255)                                                                     gTAGGCTTCCAGCGCCAGCACTTGGTAGTCGCCCCCCACCACGACCGGTACACCGAAGTCTGTGATAGTCAGCGTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAGGCCGGCCAGGTGATGCTGGTGAACGCACGcc                                                                               <  1:231783/146‑1 (MQ=255)                                                                     gTAGGCTTCCAGCGCCAGCACTTGGTAGTCGCCCCCCACCACGACCGGTACACCGAAGTCTGTGATAGTCAGCGTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAGGCCGGCCAGGTGATGCTGGTGAACGCACGCCAc                                                                             >  8:257502/1‑148 (MQ=255)                                                                        ggCTTCCAGCGCCAGCACTTGGTAGTCGCCCCCCACCACGACCGGTACACCGAAGTCTGTGATAGTCAGCGTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAGGCCGGCCAGGTGATGCTGGTGAACGCACGCCACGAGc                                                                         <  1:355790/149‑1 (MQ=255)                                                                                   ccAGCACTTGGTAGTCGCCCCCCACCACGACCGGTACACCGAAGTCTGTGATAGTCAGCGTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAGGCCGGCCAGGTGATGCTGGTGAACGCACGCCACGAGCCTGCCCCCATg                                                              >  7:278776/1‑149 (MQ=255)                                                                                      gCACTTGGTAGTCGCCCCCCACCACGACCGGTACACCGAAGTCTGTGATAGTCAGCGTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAGGCCGGCCAGGTGATGCTGGTGAACGCACGCCACGAGCCTGCCCCCATGCTg                                                           >  5:187720/1‑149 (MQ=255)                                                                                       cACTTGGTAGTCGCCCCCCACCACGACCGGTACACCGAAGTCTGTGATAGTCAGCGTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGATGCCGGCCAGGTGATGCTGGTGAacgcac                                                                                  <  2:143282/125‑1 (MQ=255)                                                                                       cACTTGGTAGTCGCCCCCCACCACGACCGGTACACCGAAGTCTGTGATAGTCAGCGTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAGGCCGGCCAGGTGATGCTGGTGAacgcac                                                                                  >  1:143282/1‑125 (MQ=255)                                                                                        aCTTGGTAGTCGCCCCCCACCACGACCGGTACACCGAAGTCTGTGATAGTCAGCGTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAGGCCGGCCAGGTGATGCTGGTGAACGCACGCCACGAGCCTGCCCCCATGCTg                                                           >  2:361117/1‑147 (MQ=255)                                                                                                 tCGCCCCCCACCACGACCGGTACACCGAAGTCTGTGATAGTCAGCGTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAGGCCGGCCAGGTGATGCTGGTGAACGCACGCCACGAGCCTGCCCCCATGCTGGAGGCCGCATc                                                <  7:257502/149‑1 (MQ=255)                                                                                                    ccccccACCACGACCGGTACACCGAAGTCTGTGATAGTCAGCGTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAGGCCGGCCAGGTGATGCTGGTGAACGCACGCCACGAGCCTGCCCCCATGCTGGAGGCCGCATCGaa                                             <  5:240158/149‑1 (MQ=255)                                                                                                     cccccACCACGACCGGTACACCGAAGTCTGTGATAGTCAGCGTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAGGCCGGCCAGGTGATGCTGGTGAACGCACGCCACGAGCCTGCCCCCATGCTGGAGGCCGCATCGaa                                             >  2:43868/1‑148 (MQ=255)                                                                                                     cccccACCACGACCGGTACACCGAAGTCTGTGATAGTCAGCGTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAGGCCGGCCAGGTGATGCTGGTGAACGCACGCCACGAGCCTGCCCCCATGCTGGAGGCCGCATCGAAc                                            <  8:195439/149‑1 (MQ=255)                                                                                                      ccccACCACGACCGGTACACCGAAGTCTGTGATAGTCAGCGTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAGGCCGGCCAGGTGATGCTGGTGAACGCACGCCACGAGCCTGCCCCCATGCTGGAGGCCGCATCGaa                                             >  1:373735/1‑147 (MQ=255)                                                                                                       cccACCACGACCGGTACACCGAAGTCTGTGATAGTCAGCGTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAGGCCGGCCAGGTGATGCTGGTGAACGCACGCCACGAGc                                                                         <  8:324220/118‑1 (MQ=255)                                                                                                       cccACCACGACCGGTACACCGAAGTCTGTGATAGTCAGCGTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAGGCCGGCCAGGTGATGCTGGTGAACGCACGCCACGAGc                                                                         >  7:324220/1‑118 (MQ=255)                                                                                                         caccacGACCGGTACACCGAAGTCTGTGATAGTCAGCGTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAGGCCGGCCAGGTGATGCTGGTGAACGCACGCCACGAGCCTGCCCCCATGCTGGAGGCCGCATCGAACAGGc                                        >  6:136467/1‑149 (MQ=255)                                                                                                         caccacGACCGGTACACCGAAGTCTGTGATAGTCAGCGTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAGGCCGGCCAGGTGATGCTGGTGAACGCACGCCACGAGCCTGCCCCCATGCTGGAGGCCGCATCGAACAGGc                                        <  5:136467/149‑1 (MQ=255)                                                                                                         caccacGACCGGTACACCGAAGTCTGTGATAGTCAGCGTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAGGCCGGCCAGGTGATGCTGGTGAACGCACGCCACGAGCCTGCCCCCATGCTGGAGGCCGCATCGAACAGGc                                        <  4:130669/149‑1 (MQ=255)                                                                                                           ccacGACCGGAACACCGCAGTCTGTGAAAGTCAGCGTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAGGCCGGCCAGGTGATGCTGGTGAACGCACGCCACGAGCCTGCCCCCATGCTGGAGGCCGCATCGAACAGGCGa                                      <  8:169630/149‑1 (MQ=255)                                                                                                                        acCGAAGTCTGTGATAGTCAGCGTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAGGCCGGCCAGGTGATGCTGGTGAACGCACGCCACGAGCCTGCCCCCATGCTGGAGGCCGCATCGAACa                                           >  3:42574/1‑131 (MQ=255)                                                                                                                        acCGAAGTCTGTGATAGTCAGCGTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAGGCCGGCCAGGTGATGCTGGTGAACGCACGCCACGAGCCTGCCCCCATGCTGGAGGCCGCATCGAACa                                           <  4:42574/131‑1 (MQ=255)                                                                                                                        acCGAAGTCTGTGATAGTCAGCGTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAGGCCGGCCAGGTGATGCTGGTGAACGCACGCCACGAGCCTGCCCCAATGCTGGAGGCCGCATCGAACAGGCGAGCATCGGCCAGt                          >  1:126621/1‑148 (MQ=255)                                                                                                                          cGAAGTCTGTGATAGTCAGCGTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAGGCCGGCCAGGTGATGCTGGTGAACGCACGCCACGAGCCTGCCCCCATGCTGGAGGCCGCATCGAACAGGCGAGCATCGGCCAGt                          <  3:105932/146‑1 (MQ=255)                                                                                                                          cGAAGTCTGTGATAGTCAGCGTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAGGCCGGCCAGGTGATGCTGGTGAACGCACGCCACGAGCCTGCCCCCATGCTGGAGGCCGCATCGAACAGGCGAGCATCGGCCAGt                          >  4:105932/1‑146 (MQ=255)                                                                                                                          cGAAGTCTGTGATAGTCAGCGTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAGGCCGGCCAGGTGATGCTGGTGAACGCACGCCACGAGCCTGCCCCCATGCTGGAGGCCGCATCGAACAGGCGAGCATCGGCCAGTGa                        >  4:212948/1‑148 (MQ=255)                                                                                                                           gAAGTCTGTGATAGTCAGCGTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAGGCCGGCCAGGTGATGCTGGTGAACGCACGCCACGAGCCTGCCCCCATGCTGGAGGCCGCATCGAACAGGCGAGCATCGGCCAGTGACa                      <  6:33939/149‑1 (MQ=255)                                                                                                                           gAAGTCGGTGATAGTCAGCGTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAGGCCGGCCAGGTGATGCTGGTGAACGCACGCCACGAGCCTGCCCCCATGCTGGAGGCCGCATCGAACAGGCGAGCATCGGCCAGTGACa                      <  6:39258/149‑1 (MQ=255)                                                                                                                              gTCTGTGATAGTCAGCGTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAGGCCGGCCAGGTGATGCTGGTGAACGCACGCCACGAGCCTGCCCCCATGCTGGAGGCCGCATCGAACAGGCGAGCATCGGCCAGTGACAGTg                   <  3:212948/149‑1 (MQ=255)                                                                                                                                 tgtgATAGTCAGCGTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAGGCCGGCCAGGTGATGCTGGTGAACGCACGCCACGAGCCTGCCCCCATGCTGGAGGCCGCATCGAACAGGCGAGCATCGGCCa                            >  7:272505/1‑137 (MQ=255)                                                                                                                                 tgtgATAGTCAGCGTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAGGCCGGCCAGGTGATGCTGGTGAACGCACGCCACGAGCCTGCCCCCATGCTGGAGGCCGCATCGAACAGGCGAGCATCGGCCa                            <  8:272505/137‑1 (MQ=255)                                                                                                                                 tgtgATAGTCAGCGTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAGGCCGGCCAGGTGATGCTGGTGAACGCACGCCACGAGCCTGCCCCCATGCTGGAGGCCGCATCGAACAGGCGAGCATCGGCCAGTGACAGTGcc                 >  7:76548/1‑148 (MQ=255)                                                                                                                                   tgATAGTCAGCGTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAGGCCGGCCAGGTGATGCTGGTGAACGCACGCCACGAGCCTGCCCCCATGCTGGAGGCCGCATCGAACAGGCGAGCATCGGCCAGTGACAGTGCCGAc              <  2:113574/149‑1 (MQ=255)                                                                                                                                   tgATAGTCAGCGTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAGGACGGCCAGGTGATGCTGGTGAACGCACGCCACGAGCCTGCCCCCATGCTGGAGGCCGCATCGAACAGGCGAGCATCGGCCAGTGACAGTGCCGa               >  3:141301/1‑148 (MQ=255)                                                                                                                                        gTCAGCGTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAGGCCGGCCAGGTGATGCTGGTGAACGCACGCCACGAGCCTGCCCCCATGCTGGAGGCCGCATCGAACAGGCGAGCATCGGCCAGTGACAGTGCCGAcagca          <  2:346960/148‑1 (MQ=255)                                                                                                                                         tCAGCGTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAGGCCGGCCAGGTGATGCTGGTGAACGCACGCCACGAGCCTGCCCCCATGCTGGAGGCCGCATCGAACAGGCGAGCATCGGCCAGTGACAGTGCCGACAGCAGa        <  7:248556/149‑1 (MQ=255)                                                                                                                                         tCAGCGTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAGGCCGGCCAGGTGATGCTGGTGAACGCACGCCACGAGCCTGCCCCCATGCTGGAGGCCGCATCGAACAGGCGAGCATCGGCCAGTGACAGTGCCGACAGCAGa        <  2:373735/149‑1 (MQ=255)                                                                                                                                              gTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAGGCCGGCCAGGTGATGCTGGTGAACGCACGCCACGAGCCTGCCCCCATGCTGGAGGCCGCATCGAACAGGCGAGCATCGGCCAGTGACAGTGCCGACAGCAGAatca    >  2:172730/1‑148 (MQ=255)                                                                                                                                               tGAATACCATGCAAAACGCCGCGAATACCGCCTGGCGCGAGGCCGGCCAGGTGATGCTGGTGAACGCACGCCACGAGCCTGCCCCCATGCTGGAGGCCGCATCGAACAGGCGAGCATCGGCCAGTGACAGTGCCGACAGCAGAatcatc  <  5:286885/149‑1 (MQ=255)                                                                                                                                               tGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAGGCCGGCCAGGTGATGCTGGTGAACGCACGCCACGAGCCTGCCCCCATGCTGGAGGCCGCATCGAACAGGCGAGCATCGGCCAGTGACAGTGCCGACAGCAGAatcatc  <  8:278776/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                    GCCGGCAACAGCAGCACCATGCCGATCAGCGCGCCACGGCCGAACTGTTGCTGGCCCACCACCGCCTTGTAGGCTTCCAGCGCCAGCACTTGGTAGTCGCCCCCCACCACGACCGGTACACCGAAGTCTGTGATAGTCAGCGTGAATACCAGGCAAAACGCCGCGAATACCGCCTGGCGCGAGGCCGGCCAGGTGATGCTGGTGAACGCACGCCACGAGCCTGCCCCCATGCTGGAGGCCGCATCGAACAGGCGAGCATCGGCCAGTGACAGTGCCGACAGCAGAATCATC  >  NC_002947/5860920‑5861210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑