Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,871,628 A→G 5.6% L342P (CTC→CCC) ‡ ptsP ← phosphoenolpyruvate‑dependent regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,871,6280AG 5.6% 207.9 / 2.5 71L342P (CTC→CCC) ‡ptsPphosphoenolpyruvate‑dependent regulator
Reads supporting (aligned to +/- strand):  ref base A (36/31);  new base G (2/2);  total (38/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.68e-01

TTCAATACCGTCGACCTTGGAGTATGGCAGGTCGACCAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGCTCACGCAGGTAGTCGTCGTCCAT  >  NC_002947/5871480‑5871776
                                                                                                                                                    |                                                                                                                                                    
ttCAATACCGTCGACCTTGGAGTATGGCAGGTCGACCAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGa                                                                                                                                                      <  6:96942/149‑1 (MQ=255)
  cAATACCGTCGACCTTGGAGTATGGCAGGTCGACCAGGCCCATCACCGTGGGAATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAg                                                                                                                                                     <  8:133219/148‑1 (MQ=255)
          tCGACCGTGGAGTATGGCAGGTCGACCAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGCTGGCGCCATGGGAGGTGCCCGAGCCGAGTACCGAGACCAGGCCGCCCAGCTTGCCTTCCGGCCCTTCACCGAGCATGGCCgg                                                                                                                                            >  6:116810/1‑149 (MQ=255)
            gACCTTGGAGTATGGCAGGTCGACCAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGgtg                                                                                                                                          >  5:260098/1‑149 (MQ=255)
              ccTTGGAGTATGGCAGGTCGACCAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGGCCATGCTCGgtgaa                                                                                                                                        <  3:271110/149‑3 (MQ=255)
              ccTTGGAGTATGGCAGGTCGACCAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGGCCATGCTCGgtgaa                                                                                                                                        <  1:365174/149‑3 (MQ=255)
               cTTGGAGTATGGCAGGTCGACCAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGgtgt                                                                                                                                         >  2:157547/1‑147 (MQ=255)
               cTTGGAGTATGGCAGGTCGACCAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGACGATGGCGCCATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGgtgt                                                                                                                                         >  6:384127/1‑147 (MQ=255)
                  ggAGTATGGCAGGTCGACCAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGt                                                                                                                                     <  3:58584/148‑1 (MQ=255)
                     gTATGGCAGGTCGACCAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCt                                                                                                                                 <  1:69527/149‑1 (MQ=255)
                         ggCAGGTCGACCAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACt                                                                                                                             >  1:225088/1‑149 (MQ=255)
                          gCAGGTCGACCAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCa                                                                                                                                       <  7:119469/138‑1 (MQ=255)
                          gCAGGTCGACCAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCa                                                                                                                                       >  8:119469/1‑138 (MQ=255)
                          gCAGGTCGACCAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACt                                                                                                                             <  3:322676/148‑1 (MQ=255)
                          gCAGGTCGACCAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACt                                                                                                                             >  2:225976/1‑148 (MQ=255)
                               tCGACCAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAg                                                                                                                       >  5:338669/1‑149 (MQ=255)
                                   ccAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATg                                                                                                                   >  2:255175/1‑149 (MQ=255)
                                    cAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATgg                                                                                                                  <  8:72477/149‑1 (MQ=255)
                                    cAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATgg                                                                                                                  >  5:35211/1‑149 (MQ=255)
                                           aTCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTc                                                                                                           >  7:267686/1‑149 (MQ=255)
                                              aCCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGc                                                                                                        <  1:225976/149‑1 (MQ=255)
                                                 gTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATa                                                                                                     >  6:336334/1‑149 (MQ=255)
                                                  tGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGACTTCCGTCACTTCACCGAGCTTGGCCGTTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATac                                                                                                    <  8:125392/149‑1 (MQ=255)
                                                     ggATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATacac                                                                                                  >  2:87214/1‑148 (MQ=255)
                                                             aTGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTg                                                                                         >  4:342793/1‑149 (MQ=255)
                                                                 cccGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAg                                                                                     <  3:342793/149‑1 (MQ=255)
                                                                     cgcgAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAgcgcg                                                                                 <  5:384127/149‑1 (MQ=255)
                                                                      gcgAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAgcgcgc                                                                                <  3:339623/149‑1 (MQ=255)
                                                                         aGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTc                                                                             <  2:225088/149‑1 (MQ=255)
                                                                          ggATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTcc                                                                            <  1:157547/149‑1 (MQ=255)
                                                                           gATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACGAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCt                                                                           <  8:267686/149‑1 (MQ=255)
                                                                           gATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCt                                                                           <  1:87214/149‑1 (MQ=255)
                                                                                                  agccgagTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGgcgc                                                    <  7:147998/149‑1 (MQ=255)
                                                                                                     cgagTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTCCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGccc                                                  >  3:349901/1‑148 (MQ=255)
                                                                                                      gagTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCa                                                <  6:338669/149‑1 (MQ=255)
                                                                                                       agTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAg                                               <  6:35211/149‑1 (MQ=255)
                                                                                                       agTACCGAGACCAGGCCGACCAGCTTGCCTTCCCGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAg                                               <  6:372907/149‑1 (MQ=255)
                                                                                                           ccGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTcc                                           <  3:262035/149‑1 (MQ=255)
                                                                                                            cGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCt                                          >  4:88404/1‑149 (MQ=255)
                                                                                                            cGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCt                                          >  1:71083/1‑149 (MQ=255)
                                                                                                            cGAGACCAGGCCGACCAGCTTGCCTTCCGGCACATCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCt                                          >  5:336211/1‑149 (MQ=255)
                                                                                                            cGAGACCAGGCCGAACACCTTCCCTTCCGGCACATCAAAGAGCAAGGAAGGAGTAAGTTCCTCACTGACCAGCATGGTCTTGTCGGCATACACCACCGACTACGCCAGCGCATCCTGCACGTAGGCCAAAAGGCGGCGCCCCAGGACCt                                          >  8:230238/1‑149 (MQ=255)
                                                                                                              agaCCAGGCCGACCAGCTTGCCTTCCGTCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTg                                        <  1:380214/149‑1 (MQ=255)
                                                                                                               gaCCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGa                                       <  1:255175/149‑1 (MQ=255)
                                                                                                                  cAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGTGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCtt                                         >  1:185191/1‑144 (MQ=255)
                                                                                                                  cAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGTGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCtt                                         <  2:185191/144‑1 (MQ=255)
                                                                                                                  cAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGt                                    >  8:327396/1‑149 (MQ=255)
                                                                                                                    ggCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCg                                  >  3:168440/1‑149 (MQ=255)
                                                                                                                    ggCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCg                                  >  5:310174/1‑149 (MQ=255)
                                                                                                                     gCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCtt                                         <  5:134121/141‑1 (MQ=255)
                                                                                                                     gCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCtt                                         >  6:134121/1‑141 (MQ=255)
                                                                                                                     gCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCg                                  >  3:289402/1‑148 (MQ=255)
                                                                                                                       cGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCaaa                                                             <  1:350451/119‑1 (MQ=255)
                                                                                                                       cGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCaaa                                                             >  2:350451/1‑119 (MQ=255)
                                                                                                                       cGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTc                                   >  4:313880/1‑145 (MQ=255)
                                                                                                                       cGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTc                                   <  3:313880/145‑1 (MQ=255)
                                                                                                                                 gCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGCTCAc                     >  1:349900/1‑149 (MQ=255)
                                                                                                                                   cTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCTGCATACACCAGCGACTGCGa                                                                                      <  2:151801/82‑1 (MQ=255)
                                                                                                                                   cTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGa                                                                                      >  1:151801/1‑82 (MQ=255)
                                                                                                                                   cTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGCTCACg                    >  1:290923/1‑148 (MQ=255)
                                                                                                                                    ttCCGGCACTTCACCGAGCATGGCCGGTGTCGGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGCTCACg                    >  3:181078/1‑147 (MQ=255)
                                                                                                                                      ccGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGCTCACGCAgg                >  2:162745/1‑149 (MQ=255)
                                                                                                                                      ccGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGCTCACGCAg                 <  2:290923/148‑1 (MQ=255)
                                                                                                                                         gCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGCTCACGCAGGTAg             <  5:112260/149‑1 (MQ=255)
                                                                                                                                            tgacACCGGCCATGCTCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGCTCACGCAGGTAgtcg          >  2:366477/4‑149 (MQ=255)
                                                                                                                                            cTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGCTCACGCAGGTAgtcg          <  2:233754/149‑1 (MQ=255)
                                                                                                                                             ttCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGCTCACGCAGGTAgtcgt         >  8:318402/1‑149 (MQ=255)
                                                                                                                                             gacACCGGCCATGCTCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGCTCACGCAGGTAgtcgt         >  6:78124/3‑149 (MQ=255)
                                                                                                                                               cACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGCTCACGCAGGTAgtcgtcg       >  7:60826/1‑149 (MQ=255)
                                                                                                                                                aCCGAGCATGGCCGGTGTCAGTCCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGCTCACGCAGGTAgtcgtcgt      <  4:349901/149‑1 (MQ=255)
                                                                                                                                                  cGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGCTCACGCAGGTAgtcgtcgtc     >  6:5965/1‑148 (MQ=255)
                                                                                                                                                   gAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGCTCACGCAGGTAGTCGTCGTCCa   >  5:285550/1‑149 (MQ=255)
                                                                                                                                                    aGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGCTCACGCAGGTAGTCGTCGTCCAt  <  2:349900/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TTCAATACCGTCGACCTTGGAGTATGGCAGGTCGACCAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGCTCACGCAGGTAGTCGTCGTCCAT  >  NC_002947/5871480‑5871776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑