Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,871,634 G→C 5.6% A340G (GCC→GGC)  ptsP ← phosphoenolpyruvate‑dependent regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,871,6340GC 5.6% 219.7 / 3.1 72A340G (GCC→GGC) ptsPphosphoenolpyruvate‑dependent regulator
Reads supporting (aligned to +/- strand):  ref base G (36/32);  new base C (2/2);  total (38/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.88e-01

TCGACCTTGGAGTATGGCAGGTCGACCAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGCTCACGCAGGTAGTCGTCGTCCATC  >  NC_002947/5871490‑5871777
                                                                                                                                                |                                                                                                                                               
tCGACCGTGGAGTATGGCAGGTCGACCAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGCTGGCGCCATGGGAGGTGCCCGAGCCGAGTACCGAGACCAGGCCGCCCAGCTTGCCTTCCGGCCCTTCACCGAGCATGGCCgg                                                                                                                                             >  6:116810/1‑149 (MQ=255)
  gACCTTGGAGTATGGCAGGTCGACCAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGgtg                                                                                                                                           >  5:260098/1‑149 (MQ=255)
    ccTTGGAGTATGGCAGGTCGACCAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGGCCATGCTCGgtgaa                                                                                                                                         <  3:271110/149‑3 (MQ=255)
    ccTTGGAGTATGGCAGGTCGACCAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGGCCATGCTCGgtgaa                                                                                                                                         <  1:365174/149‑3 (MQ=255)
     cTTGGAGTATGGCAGGTCGACCAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGgtgt                                                                                                                                          >  2:157547/1‑147 (MQ=255)
     cTTGGAGTATGGCAGGTCGACCAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGACGATGGCGCCATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGgtgt                                                                                                                                          >  6:384127/1‑147 (MQ=255)
        ggAGTATGGCAGGTCGACCAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGt                                                                                                                                      <  3:58584/148‑1 (MQ=255)
           gTATGGCAGGTCGACCAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCt                                                                                                                                  <  1:69527/149‑1 (MQ=255)
               ggCAGGTCGACCAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACt                                                                                                                              >  1:225088/1‑149 (MQ=255)
                gCAGGTCGACCAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCa                                                                                                                                        >  8:119469/1‑138 (MQ=255)
                gCAGGTCGACCAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCa                                                                                                                                        <  7:119469/138‑1 (MQ=255)
                gCAGGTCGACCAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACt                                                                                                                              <  3:322676/148‑1 (MQ=255)
                gCAGGTCGACCAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACt                                                                                                                              >  2:225976/1‑148 (MQ=255)
                     tCGACCAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAg                                                                                                                        >  5:338669/1‑149 (MQ=255)
                         ccAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATg                                                                                                                    >  2:255175/1‑149 (MQ=255)
                          cAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATgg                                                                                                                   >  5:35211/1‑149 (MQ=255)
                          cAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATgg                                                                                                                   <  8:72477/149‑1 (MQ=255)
                                 aTCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTc                                                                                                            >  7:267686/1‑149 (MQ=255)
                                    aCCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGc                                                                                                         <  1:225976/149‑1 (MQ=255)
                                       gTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATa                                                                                                      >  6:336334/1‑149 (MQ=255)
                                        tGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGACTTCCGTCACTTCACCGAGCTTGGCCGTTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATac                                                                                                     <  8:125392/149‑1 (MQ=255)
                                           ggATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATacac                                                                                                   >  2:87214/1‑148 (MQ=255)
                                                   aTGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTg                                                                                          >  4:342793/1‑149 (MQ=255)
                                                       cccGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAg                                                                                      <  3:342793/149‑1 (MQ=255)
                                                           cgcgAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAgcgcg                                                                                  <  5:384127/149‑1 (MQ=255)
                                                            gcgAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAgcgcgc                                                                                 <  3:339623/149‑1 (MQ=255)
                                                               aGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTc                                                                              <  2:225088/149‑1 (MQ=255)
                                                                ggATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTcc                                                                             <  1:157547/149‑1 (MQ=255)
                                                                 gATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACGAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCt                                                                            <  8:267686/149‑1 (MQ=255)
                                                                 gATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCt                                                                            <  1:87214/149‑1 (MQ=255)
                                                                                        agccgagTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGgcgc                                                     <  7:147998/149‑1 (MQ=255)
                                                                                           cgagTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTCCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGccc                                                   >  3:349901/1‑148 (MQ=255)
                                                                                            gagTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCa                                                 <  6:338669/149‑1 (MQ=255)
                                                                                             agTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAg                                                <  6:35211/149‑1 (MQ=255)
                                                                                             agTACCGAGACCAGGCCGACCAGCTTGCCTTCCCGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAg                                                <  6:372907/149‑1 (MQ=255)
                                                                                                 ccGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTcc                                            <  3:262035/149‑1 (MQ=255)
                                                                                                  cGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCt                                           >  4:88404/1‑149 (MQ=255)
                                                                                                  cGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCt                                           >  1:71083/1‑149 (MQ=255)
                                                                                                  cGAGACCAGGCCGACCAGCTTGCCTTCCGGCACATCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCt                                           >  5:336211/1‑149 (MQ=255)
                                                                                                  cGAGACCAGGCCGAACACCTTCCCTTCCGGCACATCAAAGAGCAAGGAAGGAGTAAGTTCCTCACTGACCAGCATGGTCTTGTCGGCATACACCACCGACTACGCCAGCGCATCCTGCACGTAGGCCAAAAGGCGGCGCCCCAGGACCt                                           >  8:230238/1‑149 (MQ=255)
                                                                                                    agaCCAGGCCGACCAGCTTGCCTTCCGTCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTg                                         <  1:380214/149‑1 (MQ=255)
                                                                                                     gaCCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGa                                        <  1:255175/149‑1 (MQ=255)
                                                                                                        cAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGTGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCtt                                          >  1:185191/1‑144 (MQ=255)
                                                                                                        cAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGTGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCtt                                          <  2:185191/144‑1 (MQ=255)
                                                                                                        cAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGt                                     >  8:327396/1‑149 (MQ=255)
                                                                                                          ggCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCg                                   >  5:310174/1‑149 (MQ=255)
                                                                                                          ggCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCg                                   >  3:168440/1‑149 (MQ=255)
                                                                                                           gCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCtt                                          <  5:134121/141‑1 (MQ=255)
                                                                                                           gCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCtt                                          >  6:134121/1‑141 (MQ=255)
                                                                                                           gCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCg                                   >  3:289402/1‑148 (MQ=255)
                                                                                                             cGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCaaa                                                              >  2:350451/1‑119 (MQ=255)
                                                                                                             cGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCaaa                                                              <  1:350451/119‑1 (MQ=255)
                                                                                                             cGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTc                                    >  4:313880/1‑145 (MQ=255)
                                                                                                             cGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTc                                    <  3:313880/145‑1 (MQ=255)
                                                                                                                       gCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGCTCAc                      >  1:349900/1‑149 (MQ=255)
                                                                                                                         cTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCTGCATACACCAGCGACTGCGa                                                                                       <  2:151801/82‑1 (MQ=255)
                                                                                                                         cTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGa                                                                                       >  1:151801/1‑82 (MQ=255)
                                                                                                                         cTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGCTCACg                     >  1:290923/1‑148 (MQ=255)
                                                                                                                          ttCCGGCACTTCACCGAGCATGGCCGGTGTCGGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGCTCACg                     >  3:181078/1‑147 (MQ=255)
                                                                                                                            ccGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGCTCACGCAgg                 >  2:162745/1‑149 (MQ=255)
                                                                                                                            ccGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGCTCACGCAg                  <  2:290923/148‑1 (MQ=255)
                                                                                                                               gCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGCTCACGCAGGTAg              <  5:112260/149‑1 (MQ=255)
                                                                                                                                  tgacACCGGCCATGCTCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGCTCACGCAGGTAgtcg           >  2:366477/4‑149 (MQ=255)
                                                                                                                                  cTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGCTCACGCAGGTAgtcg           <  2:233754/149‑1 (MQ=255)
                                                                                                                                   ttCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGCTCACGCAGGTAgtcgt          >  8:318402/1‑149 (MQ=255)
                                                                                                                                   gacACCGGCCATGCTCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGCTCACGCAGGTAgtcgt          >  6:78124/3‑149 (MQ=255)
                                                                                                                                     cACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGCTCACGCAGGTAgtcgtcg        >  7:60826/1‑149 (MQ=255)
                                                                                                                                      aCCGAGCATGGCCGGTGTCAGTCCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGCTCACGCAGGTAgtcgtcgt       <  4:349901/149‑1 (MQ=255)
                                                                                                                                        cGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGCTCACGCAGGTAgtcgtcgtc      >  6:5965/1‑148 (MQ=255)
                                                                                                                                         gAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGCTCACGCAGGTAGTCGTCGTCCa    >  5:285550/1‑149 (MQ=255)
                                                                                                                                          aGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGCTCACGCAGGTAGTCGTCGTCCAt   <  2:349900/149‑1 (MQ=255)
                                                                                                                                           gCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGCTCACGCAGGTAGTCGTCGTCCATc  <  6:336211/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                               
TCGACCTTGGAGTATGGCAGGTCGACCAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGCTCACGCAGGTAGTCGTCGTCCATC  >  NC_002947/5871490‑5871777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑