Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,886,769	C→G	7.3%	A83P (GCA→CCA)	PP_5161 ←	lysophospholipase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,886,769	0	C	G	7.3%	214.6 / 6.0	68	A83P (GCA→CCA)	PP_5161	lysophospholipase
Reads supporting (aligned to +/- strand):  ref base C (33/30);  new base G (2/3);  total (35/33)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.16e-01

GACAGGCCGTGGCCGGGCAGGTCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACGGCGCCAGGCGGGTGCGCAGGCGG  >  NC_002947/5886624‑5886917                                                                                                                                                  |                                                                                                                                                     gACAGGCCGTGGCCGGGCAGGTCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGCAACAGCGTCGCGCTTAGCTGCTCGGGCAGACAtgcctg                                                                                                                                                   >  4:22378/1‑149 (MQ=255)  aCAGGCCGTGGCCGGGCAGGTCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCAtgcctgc                                                                                                                                                  <  2:211283/149‑1 (MQ=255)   cAGGCCGTGGCCGGGCAGGTCAGAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCAtgcctgcc                                                                                                                                                 >  7:55594/1‑149 (MQ=255)    aGGCCGTGGCCGGGCAGGTCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGccc                                                                                                                                                >  4:64932/1‑149 (MQ=255)    aGGCCGTGGCCGGGCAGGTCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGccc                                                                                                                                                >  4:311253/1‑149 (MQ=255)        cGTGGCCGGGCAGGTCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGAc                                                                                                                                             >  1:25268/1‑148 (MQ=255)         gTGGCCGGGCAGGTCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGGCATGGCTGCCCGAGCa                                                                                                                                           <  1:204077/149‑1 (MQ=255)             ccGGGCAGGTCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTc                                                                                                                                       <  3:311253/149‑1 (MQ=255)              cGGGCAGGTCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTc                                                                                                                                       >  3:180547/1‑148 (MQ=255)              cGGGCAGGTCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTc                                                                                                                                       >  6:303740/1‑148 (MQ=255)                      tCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCAtgcctg                                                                                                                                                   <  1:148675/128‑1 (MQ=255)                       cacaGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCAtgc                                                                                                                                                      >  3:214219/1‑124 (MQ=255)                       cacaGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCAtgc                                                                                                                                                      <  4:214219/124‑1 (MQ=255)                        acaGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTg                                                                                                                            >  3:258542/1‑149 (MQ=255)                          aGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGAc                                                                                                                                             <  4:326785/130‑1 (MQ=255)                          aGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGAc                                                                                                                                             >  3:326785/1‑130 (MQ=255)                          aGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGcc                                                                                                                          <  6:286391/149‑1 (MQ=255)                               aTCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGa                                                                                                                       <  3:64932/147‑1 (MQ=255)                                    ggCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTAc                                                                                                                >  3:254644/1‑149 (MQ=255)                                    ggCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTAc                                                                                                                >  1:378565/1‑149 (MQ=255)                                       gAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCt                                                                                                                         >  4:241466/1‑137 (MQ=255)                                       gAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCt                                                                                                                         <  3:241466/137‑1 (MQ=255)                                               gCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGTGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGc                                                                                                                           <  4:10191/127‑1 (MQ=255)                                               gCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGTGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGc                                                                                                                           >  3:10191/1‑127 (MQ=255)                                                    aGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGc                                                                                                >  1:218284/1‑149 (MQ=255)                                                        cccACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCgg                                                                                             >  2:287786/1‑148 (MQ=255)                                                         ccACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCgg                                                                                             >  2:55539/1‑147 (MQ=255)                                                             tCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGtt                                                                                       >  7:311405/1‑149 (MQ=255)                                                                aTCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAg                                                                                    <  4:180547/149‑1 (MQ=255)                                                                 tCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGGCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAgg                                                                                   <  5:363863/149‑1 (MQ=255)                                                                   acaTGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGc                                                                                  >  1:291105/1‑148 (MQ=255)                                                                   acaTGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCAGTGTGCCGGCAGGTTCAGGc                                                                                  >  4:222544/1‑148 (MQ=255)                                                                    caTGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCa                                                                                <  1:55539/149‑1 (MQ=255)                                                                    caTGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCa                                                                                <  3:22378/149‑1 (MQ=255)                                                                      tGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCAt                                                                               >  2:301132/1‑148 (MQ=255)                                                                       gTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAAccc                                                                                                                                 >  7:216316/1‑97 (MQ=255)                                                                       gTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAAccc                                                                                                                                 <  8:216316/97‑1 (MQ=255)                                                                          ggTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGaa                                                                           <  2:25268/148‑1 (MQ=255)                                                                          ggTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGaa                                                                           <  1:146532/148‑1 (MQ=255)                                                                           gTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAgc                                                                         >  2:286485/1‑149 (MQ=255)                                                                               agGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTg                                                                     <  2:16842/149‑1 (MQ=255)                                                                                ggCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTgg                                                                    <  2:291105/149‑1 (MQ=255)                                                                                  cccATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTa                                                                  <  8:311405/149‑1 (MQ=255)                                                                                  cccATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCCGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTa                                                                  <  3:222544/149‑1 (MQ=255)                                                                                   ccATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACcca                                                                                                             >  6:227563/1‑105 (MQ=255)                                                                                   ccATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACcca                                                                                                             <  5:227563/105‑1 (MQ=255)                                                                                   ccATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAg                                                                 >  3:182321/1‑149 (MQ=255)                                                                                    cATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACccagccag                                                                                                        >  7:228425/1‑109 (MQ=255)                                                                                    cATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACccagccag                                                                                                        <  8:228425/109‑1 (MQ=255)                                                                                           tCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTgcgc                                                         >  8:8052/1‑149 (MQ=255)                                                                                                aTAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAg                                                                                    <  8:200688/117‑1 (MQ=255)                                                                                                aTAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAg                                                                                    >  7:200688/1‑117 (MQ=255)                                                                                                aTAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTtgtg                                                    <  5:303740/149‑1 (MQ=255)                                                                                                 tAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTgg                                                                    <  7:161144/132‑1 (MQ=255)                                                                                                 tAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCAGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTgg                                                                    >  8:161144/1‑132 (MQ=255)                                                                                                   gCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGcc                                                  >  1:250865/1‑148 (MQ=255)                                                                                                      gTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTACCGTCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACa                                              <  8:55594/149‑1 (MQ=255)                                                                                                        gcagcaGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGt                                            >  4:65200/1‑149 (MQ=255)                                                                                                         cagcagGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGt                                            <  1:301132/148‑1 (MQ=255)                                                                                                               gAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGtggctggc                                     >  1:276/1‑149 (MQ=255)                                                                                                                   aGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAgt                                 <  2:378565/149‑1 (MQ=255)                                                                                                                      gTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAgtggt                              >  1:300011/1‑149 (MQ=255)                                                                                                                      gTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAgtggt                              >  5:141061/1‑149 (MQ=255)                                                                                                                         gcgcTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGACCCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGaa                           <  8:194964/149‑1 (MQ=255)                                                                                                                           gcTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACg                         <  4:182321/149‑1 (MQ=255)                                                                                                                             atGGCTGCCCGAGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACGgc                       >  2:109126/2‑149 (MQ=255)                                                                                                                               acctggtCGGGCAGGCATGGCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAgtg                                <  6:72609/136‑1 (MQ=255)                                                                                                                               acctggtCGGGCAGGCATGGCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAgtg                                >  5:72609/3‑138 (MQ=255)                                                                                                                                 ctggtCGGGCAGGCATGGCTGCCCGAGCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACGgcgc                     >  6:73145/1‑147 (MQ=255)                                                                                                                                      cGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACGGCGCCAGGCgg              <  4:258542/149‑1 (MQ=255)                                                                                                                                       gggCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACGGCGCCAGGCgg              <  1:261177/148‑1 (MQ=255)                                                                                                                                        ggCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACGGCGCCAGGCGGGt            >  6:189697/1‑149 (MQ=255)                                                                                                                                        ggCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACGGCGCCAGGCGGGt            <  4:116766/149‑1 (MQ=255)                                                                                                                                          cAGGCATGGCTGCCCGAGCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACGGCGCCAGGCGGGTgc          <  5:73145/149‑1 (MQ=255)                                                                                                                                                tgcctgccCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACGGCGCCAGGCGGGTGCGCAGGc    <  5:189697/149‑1 (MQ=255)                                                                                                                                                  cctgccCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACGGCGCCAGGCGGGTGCGCAGGCgg  <  2:218284/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                     GACAGGCCGTGGCCGGGCAGGTCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACGGCGCCAGGCGGGTGCGCAGGCGG  >  NC_002947/5886624‑5886917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑