Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,898,904	G→C	7.3%	R662G (CGG→GGG)	PP_5173 ←	RND family transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,898,904	0	G	C	7.3%	245.4 / 5.2	78	R662G (CGG→GGG)	PP_5173	RND family transporter
Reads supporting (aligned to +/- strand):  ref base G (34/38);  new base C (3/3);  total (37/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01

ATTTCCACGCGCAGCACCTTACCCGGCTCGTTCCAGTCGTAGATCATCTCGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTGACAATCACCAGCTGCGCGTAGTAAGGG  >  NC_002947/5898756‑5899049                                                                                                                                                       |                                                                                                                                                  aTTTCCACGCGCAGCACCTTACCCGGCTCGTTCCAGTCGTAGATCATCTCGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATcggccg                                                                                                                                                   >  5:313305/1‑149 (MQ=255)             agcaCCTTACCCGGCTCGTTCCAGTCGTAGATCATCTCGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACcggcgg                                                                                                                                       <  5:52661/149‑1 (MQ=255)               caCCTTACCCGGCTCGTTCCAGTCGTAGATCATCTCGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGc                                                                                                                                     <  1:197592/149‑1 (MQ=255)                 ccTTACCCGGCTCGTTCCAGTCGTAGATCATCTCGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGcc                                                                                                                                    >  1:278363/1‑148 (MQ=255)                      cccGGCTCGTTCCAGTCGTAGATCATCTCGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCAtct                                                                                                                               <  1:361341/148‑1 (MQ=255)                       ccGGCTCGTTCCAGTCGTAGATCATCTCGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCAtctc                                                                                                                              >  5:4754/1‑148 (MQ=255)                        cGGCTCGTTCCAGTCGTAGATCATCTCGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGGCTCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTcc                                                                                                                             <  1:109155/149‑1 (MQ=255)                          gCTCGTTCCAGTCGTAGATCATCTCGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGc                                                                                                                          <  1:275407/149‑1 (MQ=255)                              gTTCCAGTCGTAGATCATCTCGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGAtt                                                                                                                      >  8:182815/1‑149 (MQ=255)                                  cAGTCGTAGATCATCTCGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCAc                                                                                                                  >  6:217952/1‑149 (MQ=255)                                    gTCGTAGATCATCTCGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGt                                                                                                                >  4:135014/1‑149 (MQ=255)                                          gATCATCTCGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTgc                                                                                                          <  6:313305/149‑1 (MQ=255)                                              atcTCGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTgccgac                                                                                                      >  1:193861/1‑149 (MQ=255)                                                 tcGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTgccgacgcc                                                                                                   >  3:282536/1‑149 (MQ=255)                                                  cGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTgccgacgcc                                                                                                   >  6:350008/1‑148 (MQ=255)                                                   gCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTgccgacgc                                                                                                    <  5:217952/146‑1 (MQ=255)                                                         gtgCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAAtt                                                                                           >  1:71517/1‑149 (MQ=255)                                                           gCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAAttct                                                                                         >  8:186575/1‑149 (MQ=255)                                                           gCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAAttct                                                                                         >  7:130137/1‑149 (MQ=255)                                                            cTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAAttct                                                                                         >  6:2079/1‑148 (MQ=255)                                                              cGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCa                                                                                      <  3:86586/149‑1 (MQ=255)                                                               gTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCa                                                                                      <  7:266719/148‑1 (MQ=255)                                                               gTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCAt                                                                                     >  3:200571/1‑149 (MQ=255)                                                                ttCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGAtt                                                                                                                      <  8:176315/115‑1 (MQ=255)                                                                ttCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGAtt                                                                                                                      >  7:176315/1‑115 (MQ=255)                                                                     gTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCa                                                                                  <  5:253728/146‑1 (MQ=255)                                                                     gTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCa                                                                                  <  4:282536/146‑1 (MQ=255)                                                                     gTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGa                                                                               >  5:286706/1‑149 (MQ=255)                                                                     gTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGa                                                                               >  6:133307/1‑149 (MQ=255)                                                                          gCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGAtcttc                                                                          <  5:143026/149‑1 (MQ=255)                                                                            aaGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTg                                                                        <  8:29567/149‑1 (MQ=255)                                                                             aGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGGCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGc                                                                       <  8:130137/149‑1 (MQ=255)                                                                                tGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAgg                                                                    <  5:350008/149‑1 (MQ=255)                                                                                  gCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATGGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCTGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCg                                                                  <  4:30427/149‑1 (MQ=255)                                                                                  gCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCg                                                                  >  6:179459/1‑149 (MQ=255)                                                                                  gCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCg                                                                  <  2:243365/149‑1 (MQ=255)                                                                                    cAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGc                                                                                                                                     >  5:293835/1‑80 (MQ=255)                                                                                    cAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGc                                                                                                                                     <  6:293835/80‑1 (MQ=255)                                                                                    cAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCTGGATCTTCTGCAGGCGGt                                                                >  4:112274/1‑149 (MQ=255)                                                                                    cAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAAATCTTCATGCAGGATCTTCTGCAGGAGGt                                                                >  3:134641/1‑149 (MQ=255)                                                                                    cAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCCCTGACC‑CGGTACTGAAACGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGCGGCCTAGGAATTCTTCATGCAGGATCTTATGCAGGCgg                                                                 >  8:292392/1‑148 (MQ=255)                                                                                     aGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTc                                                               <  7:241513/149‑1 (MQ=255)                                                                                      gCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTc                                                               >  3:9715/1‑148 (MQ=255)                                                                                       cTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCgg                                                                 <  6:373594/145‑1 (MQ=255)                                                                                       cTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCgg                                                                 >  5:373594/1‑145 (MQ=255)                                                                                       cTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCca                                                             <  1:182814/149‑1 (MQ=255)                                                                                        tCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACCGCGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCca                                                             >  1:78030/1‑149 (MQ=255)                                                                                        tCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCca                                                             >  1:132808/1‑148 (MQ=255)                                                                                             ggCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAg                                                                     >  4:293233/1‑135 (MQ=255)                                                                                             ggCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAg                                                                     <  3:293233/135‑1 (MQ=255)                                                                                                   tttGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCACGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGa                                                  <  2:193861/148‑1 (MQ=255)                                                                                                   tttGCGCACCTGG‑TCGATGTCGGCACCGCTGAAC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCGCCTCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTGTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGAc                                                 >  2:351709/1‑149 (MQ=255)                                                                                                     tGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACg                                                <  5:2079/148‑1 (MQ=255)                                                                                                       cgcACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGctc                                             <  6:4754/149‑1 (MQ=255)                                                                                                           ccTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCg                                         <  7:186575/149‑1 (MQ=255)                                                                                                            cTGG‑TCGATGTCGGCAGCGCTGACC‑CGGTACTGAATCGTCCGGCCCACCGGCTGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTGTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTc                                          <  3:42015/147‑1 (MQ=255)                                                                                                                 tCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCCGACGCTCTTCGAAGcc                                    <  5:133307/149‑1 (MQ=255)                                                                                                                     tGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTg                                <  5:179459/149‑1 (MQ=255)                                                                                                                       tCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCt                              <  2:278363/149‑1 (MQ=255)                                                                                                                         ggCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCGCAAAATTTATTCATAAAGGATCTTATGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTg                             >  1:123756/1‑148 (MQ=255)                                                                                                                         ggCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTGa                            >  2:51113/1‑149 (MQ=255)                                                                                                                         ggCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTGa                            <  7:182815/149‑1 (MQ=255)                                                                                                                               gCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGACGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTGACAATCa                      >  5:68810/1‑149 (MQ=255)                                                                                                                                cTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTGACAATCAc                     <  2:71517/149‑1 (MQ=255)                                                                                                                                cTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTGACAATCAc                     <  4:9715/149‑1 (MQ=255)                                                                                                                                     c‑cGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTg                             >  6:331652/1‑136 (MQ=255)                                                                                                                                     c‑cGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTg                             <  5:331652/136‑1 (MQ=255)                                                                                                                                        cgctggagatgGGCCCGCCGGTGGGCCGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTGACAATCACCAGCTgc              <  4:81733‑M1/138‑1 (MQ=255)                                                                                                                                          tACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGTATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTGACAATCACCAGCTgcgc            <  2:132808/149‑1 (MQ=255)                                                                                                                                          ctggagatgGGCCCGCCGGTGGGCCGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCa                                                                      <  7:21368‑M1/82‑1 (MQ=255)                                                                                                                                          ctggagatgGGCCCGCCGGTGGGCCGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCa                                                                      >  8:21368‑M1/10‑91 (MQ=255)                                                                                                                                          ctggagatgGGCCCGCCGGTGGGCCGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCca                                                             >  5:195398‑M1/10‑100 (MQ=255)                                                                                                                                          ctggagatgGGCCCGCCGGTGGGCCGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCca                                                             <  6:195398‑M1/91‑1 (MQ=255)                                                                                                                                             tGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTGACAATCACCAGCTGcgcg           >  8:46980/1‑147 (MQ=255)                                                                                                                                               aaTCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTGACAATCACCAGCTGCGCgta         <  3:135014/147‑1 (MQ=255)                                                                                                                                                   GGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTGACAATCACCAGCTGCGCGTAGTAAgg   >  2:78030‑M1/1‑149 (MQ=255)                                                                                                                                                   GGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTGACAATCACCAGCTGCGCGTAGTAAgg   >  8:98042‑M1/1‑149 (MQ=255)                                                                                                                                                   GGCCCGCCCACCGGCGGGCCCATCTCCCGCGATTGCACGGTGGTGCCGACGCCGACGAAGTCTTCATTCCCGATCTTCTGCAGGCGCTCCATCATGGCCTGACGCTCATCGAAGCCCTTGCTGACAATCACCAGCTGCGCGTAGTAAgg   >  6:34628‑M1/1‑149 (MQ=255)                                                                                                                                                    GCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGCCGCTCTTCGAAGCCCTTGCTGACAATCACCAGCTGCGCGTAGTAAggg  <  2:188226‑M1/149‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                  ATTTCCACGCGCAGCACCTTACCCGGCTCGTTCCAGTCGTAGATCATCTCGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTGACAATCACCAGCTGCGCGTAGTAAGGG  >  NC_002947/5898756‑5899049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑