Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,898,910	C→T	6.2%	V660M (GTG→ATG) ‡	PP_5173 ←	RND family transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,898,910	0	C	T	6.2%	236.1 / 4.2	80	V660M (GTG→ATG) ‡	PP_5173	RND family transporter
Reads supporting (aligned to +/- strand):  ref base C (36/39);  new base T (2/3);  total (38/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

AGCACCTTACCCGGCTCGTTCCAGTCGTAGATCATCTCGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTGACAATCACCAGCTGCGCGTAGTAAGGGTTCTGC  >  NC_002947/5898768‑5899055                                                                                                                                                 |                                                                                                                                                  agcaCCTTACCCGGCTCGTTCCAGTCGTAGATCATCTCGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACcggcgg                                                                                                                                             <  5:52661/149‑1 (MQ=255)   caCCTTACCCGGCTCGTTCCAGTCGTAGATCATCTCGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGc                                                                                                                                           <  1:197592/149‑1 (MQ=255)     ccTTACCCGGCTCGTTCCAGTCGTAGATCATCTCGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGcc                                                                                                                                          >  1:278363/1‑148 (MQ=255)          cccGGCTCGTTCCAGTCGTAGATCATCTCGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCAtct                                                                                                                                     <  1:361341/148‑1 (MQ=255)           ccGGCTCGTTCCAGTCGTAGATCATCTCGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCAtctc                                                                                                                                    >  5:4754/1‑148 (MQ=255)            cGGCTCGTTCCAGTCGTAGATCATCTCGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGGCTCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTcc                                                                                                                                   <  1:109155/149‑1 (MQ=255)              gCTCGTTCCAGTCGTAGATCATCTCGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGc                                                                                                                                <  1:275407/149‑1 (MQ=255)                  gTTCCAGTCGTAGATCATCTCGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGAtt                                                                                                                            >  8:182815/1‑149 (MQ=255)                      cAGTCGTAGATCATCTCGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCAc                                                                                                                        >  6:217952/1‑149 (MQ=255)                        gTCGTAGATCATCTCGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGt                                                                                                                      >  4:135014/1‑149 (MQ=255)                              gATCATCTCGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTgc                                                                                                                <  6:313305/149‑1 (MQ=255)                                  atcTCGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTgccgac                                                                                                            >  1:193861/1‑149 (MQ=255)                                     tcGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTgccgacgcc                                                                                                         >  3:282536/1‑149 (MQ=255)                                      cGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTgccgacgcc                                                                                                         >  6:350008/1‑148 (MQ=255)                                       gCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTgccgacgc                                                                                                          <  5:217952/146‑1 (MQ=255)                                             gtgCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAAtt                                                                                                 >  1:71517/1‑149 (MQ=255)                                               gCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAAttct                                                                                               >  8:186575/1‑149 (MQ=255)                                               gCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAAttct                                                                                               >  7:130137/1‑149 (MQ=255)                                                cTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAAttct                                                                                               >  6:2079/1‑148 (MQ=255)                                                  cGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCa                                                                                            <  3:86586/149‑1 (MQ=255)                                                   gTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCa                                                                                            <  7:266719/148‑1 (MQ=255)                                                   gTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCAt                                                                                           >  3:200571/1‑149 (MQ=255)                                                    ttCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGAtt                                                                                                                            >  7:176315/1‑115 (MQ=255)                                                    ttCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGAtt                                                                                                                            <  8:176315/115‑1 (MQ=255)                                                         gTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCa                                                                                        <  5:253728/146‑1 (MQ=255)                                                         gTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCa                                                                                        <  4:282536/146‑1 (MQ=255)                                                         gTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGa                                                                                     >  5:286706/1‑149 (MQ=255)                                                         gTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGa                                                                                     >  6:133307/1‑149 (MQ=255)                                                              gCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGAtcttc                                                                                <  5:143026/149‑1 (MQ=255)                                                                aaGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTg                                                                              <  8:29567/149‑1 (MQ=255)                                                                 aGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGGCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGc                                                                             <  8:130137/149‑1 (MQ=255)                                                                    tGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAgg                                                                          <  5:350008/149‑1 (MQ=255)                                                                      gCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATGGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCTGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCg                                                                        <  4:30427/149‑1 (MQ=255)                                                                      gCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCg                                                                        >  6:179459/1‑149 (MQ=255)                                                                      gCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCg                                                                        <  2:243365/149‑1 (MQ=255)                                                                        cAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGc                                                                                                                                           >  5:293835/1‑80 (MQ=255)                                                                        cAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGc                                                                                                                                           <  6:293835/80‑1 (MQ=255)                                                                        cAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCTGGATCTTCTGCAGGCGGt                                                                      >  4:112274/1‑149 (MQ=255)                                                                        cAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAAATCTTCATGCAGGATCTTCTGCAGGAGGt                                                                      >  3:134641/1‑149 (MQ=255)                                                                        cAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCCCTGACC‑CGGTACTGAAACGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGCGGCCTAGGAATTCTTCATGCAGGATCTTATGCAGGCgg                                                                       >  8:292392/1‑148 (MQ=255)                                                                         aGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTc                                                                     <  7:241513/149‑1 (MQ=255)                                                                          gCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTc                                                                     >  3:9715/1‑148 (MQ=255)                                                                           cTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCgg                                                                       <  6:373594/145‑1 (MQ=255)                                                                           cTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCgg                                                                       >  5:373594/1‑145 (MQ=255)                                                                           cTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCca                                                                   <  1:182814/149‑1 (MQ=255)                                                                            tCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACCGCGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCca                                                                   >  1:78030/1‑149 (MQ=255)                                                                            tCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCca                                                                   >  1:132808/1‑148 (MQ=255)                                                                                 ggCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAg                                                                           >  4:293233/1‑135 (MQ=255)                                                                                 ggCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAg                                                                           <  3:293233/135‑1 (MQ=255)                                                                                       tttGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCACGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGa                                                        <  2:193861/148‑1 (MQ=255)                                                                                       tttGCGCACCTGG‑TCGATGTCGGCACCGCTGAAC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCGCCTCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTGTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGAc                                                       >  2:351709/1‑149 (MQ=255)                                                                                         tGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACg                                                      <  5:2079/148‑1 (MQ=255)                                                                                           cgcACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGctc                                                   <  6:4754/149‑1 (MQ=255)                                                                                               ccTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCg                                               <  7:186575/149‑1 (MQ=255)                                                                                                cTGG‑TCGATGTCGGCAGCGCTGACC‑CGGTACTGAATCGTCCGGCCCACCGGCTGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTGTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTc                                                <  3:42015/147‑1 (MQ=255)                                                                                                     tCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCCGACGCTCTTCGAAGcc                                          <  5:133307/149‑1 (MQ=255)                                                                                                         tGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTg                                      <  5:179459/149‑1 (MQ=255)                                                                                                           tCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCt                                    <  2:278363/149‑1 (MQ=255)                                                                                                             ggCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCGCAAAATTTATTCATAAAGGATCTTATGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTg                                   >  1:123756/1‑148 (MQ=255)                                                                                                             ggCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTGa                                  <  7:182815/149‑1 (MQ=255)                                                                                                             ggCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTGa                                  >  2:51113/1‑149 (MQ=255)                                                                                                                   gCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGACGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTGACAATCa                            >  5:68810/1‑149 (MQ=255)                                                                                                                    cTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTGACAATCAc                           <  4:9715/149‑1 (MQ=255)                                                                                                                    cTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTGACAATCAc                           <  2:71517/149‑1 (MQ=255)                                                                                                                         c‑cGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTg                                   >  6:331652/1‑136 (MQ=255)                                                                                                                         c‑cGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTg                                   <  5:331652/136‑1 (MQ=255)                                                                                                                            cgctggagatgGGCCCGCCGGTGGGCCGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTGACAATCACCAGCTgc                    <  4:81733‑M1/138‑1 (MQ=255)                                                                                                                              tACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGTATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTGACAATCACCAGCTgcgc                  <  2:132808/149‑1 (MQ=255)                                                                                                                              ctggagatgGGCCCGCCGGTGGGCCGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCa                                                                            >  8:21368‑M1/10‑91 (MQ=255)                                                                                                                              ctggagatgGGCCCGCCGGTGGGCCGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCa                                                                            <  7:21368‑M1/82‑1 (MQ=255)                                                                                                                              ctggagatgGGCCCGCCGGTGGGCCGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCca                                                                   >  5:195398‑M1/10‑100 (MQ=255)                                                                                                                              ctggagatgGGCCCGCCGGTGGGCCGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCca                                                                   <  6:195398‑M1/91‑1 (MQ=255)                                                                                                                                 tGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTGACAATCACCAGCTGcgcg                 >  8:46980/1‑147 (MQ=255)                                                                                                                                   aaTCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTGACAATCACCAGCTGCGCgta               <  3:135014/147‑1 (MQ=255)                                                                                                                                       GGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTGACAATCACCAGCTGCGCGTAGTAAgg         >  2:78030‑M1/1‑149 (MQ=255)                                                                                                                                       GGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTGACAATCACCAGCTGCGCGTAGTAAgg         >  8:98042‑M1/1‑149 (MQ=255)                                                                                                                                       GGCCCGCCCACCGGCGGGCCCATCTCCCGCGATTGCACGGTGGTGCCGACGCCGACGAAGTCTTCATTCCCGATCTTCTGCAGGCGCTCCATCATGGCCTGACGCTCATCGAAGCCCTTGCTGACAATCACCAGCTGCGCGTAGTAAgg         >  6:34628‑M1/1‑149 (MQ=255)                                                                                                                                        GCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGCCGCTCTTCGAAGCCCTTGCTGACAATCACCAGCTGCGCGTAGTAAggg        <  2:188226‑M1/149‑1 (MQ=255)                                                                                                                                             cccACGGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGTGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTGACAATCACCAGCTGCGCGTAGTAAGGGTTCTg   <  4:200571/149‑1 (MQ=255)                                                                                                                                               cACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTGACAATCACCAGCTGCGCGTAGTAAGGGTTCTGc  >  1:255178/1‑148 (MQ=255)                                                                                                                                                 |                                                                                                                                                  AGCACCTTACCCGGCTCGTTCCAGTCGTAGATCATCTCGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTGACAATCACCAGCTGCGCGTAGTAAGGGTTCTGC  >  NC_002947/5898768‑5899055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑