Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,898,915	G→C	6.3%	P658R (CCG→CGG)	PP_5173 ←	RND family transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,898,915	0	G	C	6.3%	242.0 / 6.1	79	P658R (CCG→CGG)	PP_5173	RND family transporter
Reads supporting (aligned to +/- strand):  ref base G (36/37);  new base C (2/3);  total (38/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.94e-01

AGCACCTTACCCGGCTCGTTCCAGTCGTAGATCATCTCGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTGACAATCACCAGCTGCGCGTAGTAAGGGTTCTGCAACTG  >  NC_002947/5898768‑5899060                                                                                                                                                      |                                                                                                                                                   agcaCCTTACCCGGCTCGTTCCAGTCGTAGATCATCTCGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACcggcgg                                                                                                                                                   <  5:52661/149‑1 (MQ=255)   caCCTTACCCGGCTCGTTCCAGTCGTAGATCATCTCGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGc                                                                                                                                                 <  1:197592/149‑1 (MQ=255)     ccTTACCCGGCTCGTTCCAGTCGTAGATCATCTCGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGcc                                                                                                                                                >  1:278363/1‑148 (MQ=255)          cccGGCTCGTTCCAGTCGTAGATCATCTCGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCAtct                                                                                                                                           <  1:361341/148‑1 (MQ=255)           ccGGCTCGTTCCAGTCGTAGATCATCTCGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCAtctc                                                                                                                                          >  5:4754/1‑148 (MQ=255)            cGGCTCGTTCCAGTCGTAGATCATCTCGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGGCTCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTcc                                                                                                                                         <  1:109155/149‑1 (MQ=255)              gCTCGTTCCAGTCGTAGATCATCTCGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGc                                                                                                                                      <  1:275407/149‑1 (MQ=255)                  gTTCCAGTCGTAGATCATCTCGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGAtt                                                                                                                                  >  8:182815/1‑149 (MQ=255)                      cAGTCGTAGATCATCTCGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCAc                                                                                                                              >  6:217952/1‑149 (MQ=255)                        gTCGTAGATCATCTCGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGt                                                                                                                            >  4:135014/1‑149 (MQ=255)                              gATCATCTCGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTgc                                                                                                                      <  6:313305/149‑1 (MQ=255)                                  atcTCGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTgccgac                                                                                                                  >  1:193861/1‑149 (MQ=255)                                     tcGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTgccgacgcc                                                                                                               >  3:282536/1‑149 (MQ=255)                                      cGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTgccgacgcc                                                                                                               >  6:350008/1‑148 (MQ=255)                                       gCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTgccgacgc                                                                                                                <  5:217952/146‑1 (MQ=255)                                             gtgCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAAtt                                                                                                       >  1:71517/1‑149 (MQ=255)                                               gCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAAttct                                                                                                     >  7:130137/1‑149 (MQ=255)                                               gCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAAttct                                                                                                     >  8:186575/1‑149 (MQ=255)                                                cTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAAttct                                                                                                     >  6:2079/1‑148 (MQ=255)                                                  cGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑a                                                                                                 <  3:86586/149‑1 (MQ=255)                                                   gTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑a                                                                                                 <  7:266719/148‑1 (MQ=255)                                                   gTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑At                                                                                                >  3:200571/1‑149 (MQ=255)                                                    ttCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGAtt                                                                                                                                  <  8:176315/115‑1 (MQ=255)                                                    ttCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGAtt                                                                                                                                  >  7:176315/1‑115 (MQ=255)                                                         gTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCa                                                                                             <  5:253728/146‑1 (MQ=255)                                                         gTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCa                                                                                             <  4:282536/146‑1 (MQ=255)                                                         gTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCAGGa                                                                                          >  5:286706/1‑149 (MQ=255)                                                         gTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCAGGa                                                                                          >  6:133307/1‑149 (MQ=255)                                                              gCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCAGGAtcttc                                                                                     <  5:143026/149‑1 (MQ=255)                                                                aaGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCAGGATCTTCTg                                                                                   <  8:29567/149‑1 (MQ=255)                                                                 aGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGGCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCAGGATCTTCTGc                                                                                  <  8:130137/149‑1 (MQ=255)                                                                    tGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCAGGATCTTCTGCAgg                                                                               <  5:350008/149‑1 (MQ=255)                                                                      gCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATGGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCTGACGCCGACGAATTCTTC‑ATGCAGGATCTTCTGCAGGCg                                                                             <  4:30427/149‑1 (MQ=255)                                                                      gCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCAGGATCTTCTGCAGGCg                                                                             <  2:243365/149‑1 (MQ=255)                                                                      gCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCAGGATCTTCTGCAGGCg                                                                             >  6:179459/1‑149 (MQ=255)                                                                        cAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGc                                                                                                                                                 <  6:293835/80‑1 (MQ=255)                                                                        cAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGc                                                                                                                                                 >  5:293835/1‑80 (MQ=255)                                                                        cAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCTGGATCTTCTGCAGGCGGt                                                                           >  4:112274/1‑149 (MQ=255)                                                                        cAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAAATCTTC‑ATGCAGGATCTTCTGCAGGAGGt                                                                           >  3:134641/1‑149 (MQ=255)                                                                        cAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCCCTGACC‑CGGTACTGAAACGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGCGGCCTAGGAATTCTTC‑ATGCAGGATCTTATGCAGGCgg                                                                            >  8:292392/1‑148 (MQ=255)                                                                         aGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCAGGATCTTCTGCAGGCGGTc                                                                          <  7:241513/149‑1 (MQ=255)                                                                          gCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCAGGATCTTCTGCAGGCGGTc                                                                          >  3:9715/1‑148 (MQ=255)                                                                           cTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCAGGATCTTCTGCAGGCgg                                                                            >  5:373594/1‑145 (MQ=255)                                                                           cTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCAGGATCTTCTGCAGGCgg                                                                            <  6:373594/145‑1 (MQ=255)                                                                           cTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCAGGATCTTCTGCAGGCGGTCca                                                                        <  1:182814/149‑1 (MQ=255)                                                                            tCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACCGCGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCAGGATCTTCTGCAGGCGGTCca                                                                        >  1:78030/1‑149 (MQ=255)                                                                            tCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCAGGATCTTCTGCAGGCGGTCca                                                                        >  1:132808/1‑148 (MQ=255)                                                                                 ggCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCAGGATCTTCTGCAg                                                                                <  3:293233/135‑1 (MQ=255)                                                                                 ggCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCAGGATCTTCTGCAg                                                                                >  4:293233/1‑135 (MQ=255)                                                                                       tttGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ACGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGa                                                             <  2:193861/148‑1 (MQ=255)                                                                                       tttGCGCACCTGG‑TCGATGTCGGCACCGCTGAAC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCGCCTCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTGTTC‑ATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGAc                                                            >  2:351709/1‑149 (MQ=255)                                                                                         tGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACg                                                           <  5:2079/148‑1 (MQ=255)                                                                                           cgcACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGctc                                                        <  6:4754/149‑1 (MQ=255)                                                                                               ccTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCg                                                    <  7:186575/149‑1 (MQ=255)                                                                                                cTGG‑TCGATGTCGGCAGCGCTGACC‑CGGTACTGAATCGTCCGGCCCACCGGCTGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTGTTC‑ATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTc                                                     <  3:42015/147‑1 (MQ=255)                                                                                                     tCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCCGACGCTCTTCGAAGcc                                               <  5:133307/149‑1 (MQ=255)                                                                                                         tGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTg                                           <  5:179459/149‑1 (MQ=255)                                                                                                           tCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCt                                         <  2:278363/149‑1 (MQ=255)                                                                                                             ggCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCGCAAAATTTATTC‑ATAAAGGATCTTATGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTg                                        >  1:123756/1‑148 (MQ=255)                                                                                                             ggCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTGa                                       <  7:182815/149‑1 (MQ=255)                                                                                                             ggCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTGa                                       >  2:51113/1‑149 (MQ=255)                                                                                                                   gCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGACGACGAATTCTTC‑ATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTGACAATCa                                 >  5:68810/1‑149 (MQ=255)                                                                                                                    cTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTGACAATCAc                                <  4:9715/149‑1 (MQ=255)                                                                                                                    cTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTGACAATCAc                                <  2:71517/149‑1 (MQ=255)                                                                                                                         c‑cGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTg                                        >  6:331652/1‑136 (MQ=255)                                                                                                                         c‑cGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTg                                        <  5:331652/136‑1 (MQ=255)                                                                                                                            cgctggagatgGGCCCGCCGGTGGGCCGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTGACAATCACCAGCTgc                         <  4:81733‑M1/138‑1 (MQ=255)                                                                                                                              tACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCAGTATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTGACAATCACCAGCTgcgc                       <  2:132808/149‑1 (MQ=255)                                                                                                                              ctggagatgGGCCCGCCGGTGGGCCGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCAGGATCTTCTGCa                                                                                 >  8:21368‑M1/10‑91 (MQ=255)                                                                                                                              ctggagatgGGCCCGCCGGTGGGCCGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCAGGATCTTCTGCa                                                                                 <  7:21368‑M1/82‑1 (MQ=255)                                                                                                                              ctggagatgGGCCCGCCGGTGGGCCGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCAGGATCTTCTGCAGGCGGTCca                                                                        >  5:195398‑M1/10‑100 (MQ=255)                                                                                                                              ctggagatgGGCCCGCCGGTGGGCCGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCAGGATCTTCTGCAGGCGGTCca                                                                        <  6:195398‑M1/91‑1 (MQ=255)                                                                                                                                 tGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTGACAATCACCAGCTGcgcg                      >  8:46980/1‑147 (MQ=255)                                                                                                                                   aaTCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTGACAATCACCAGCTGCGCgta                    <  3:135014/147‑1 (MQ=255)                                                                                                                                       GGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTGACAATCACCAGCTGCGCGTAGTAAgg              >  2:78030‑M1/1‑149 (MQ=255)                                                                                                                                       GGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTGACAATCACCAGCTGCGCGTAGTAAgg              >  8:98042‑M1/1‑149 (MQ=255)                                                                                                                                       GGCCCGCCCACCGGCGGGCCCATCTCCCGCGATTGCACGGTGGTGCCGACGCCGACGAAGTCTTC‑ATTCCCGATCTTCTGCAGGCGCTCCATCATGGCCTGACGCTCATCGAAGCCCTTGCTGACAATCACCAGCTGCGCGTAGTAAgg              >  6:34628‑M1/1‑149 (MQ=255)                                                                                                                                        GCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGCCGCTCTTCGAAGCCCTTGCTGACAATCACCAGCTGCGCGTAGTAAggg             <  2:188226‑M1/149‑1 (MQ=255)                                                                                                                                             cccACGGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCAGGATCTTCTGCAGGTGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTGACAATCACCAGCTGCGCGTAGTAAGGGTTCTg        <  4:200571/149‑1 (MQ=255)                                                                                                                                               cACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTGACAATCACCAGCTGCGCGTAGTAAGGGTTCTGc       >  1:255178/1‑148 (MQ=255)                                                                                                                                                    gcggGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTCGATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTGACAATCACCAGCTGCGCGTAGTAAGGGTTCTGCAActg  >  1:288494/1‑149 (MQ=255)                                                                                                                                                      |                                                                                                                                                   AGCACCTTACCCGGCTCGTTCCAGTCGTAGATCATCTCGCCGATGTGCTCGTTCTGGTCGAGCAAGGTGGCCAGCTCGATGGCGTGTTTGCGCACCTGG‑TCGATGTCGGCACCGCTGACC‑CGGTACTGAATCGGCCGGCCCACCGGCGGGCCCATCTCCAGCGATTGCACGTTGGTGCCGACGCCGACGAATTCTTC‑ATGCAGGATCTTCTGCAGGCGGTCCATCATGGCCTGACGCTCTTCGAAGCCCTTGCTGACAATCACCAGCTGCGCGTAGTAAGGGTTCTGCAACTG  >  NC_002947/5898768‑5899060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑