Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,903,164 G→T 11.6% intergenic (‑128/‑140) PP_5175 ← / → PP_5176 HlyD family secretion protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,903,1640GT11.6% 86.6 / 2.2 40intergenic (‑128/‑140)PP_5175/PP_5176HlyD family secretion protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (12/22);  new base T (3/2);  total (16/24)
Fisher's exact test for biased strand distribution p-value = 3.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTG‑CCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCAT‑CCCCGTTCCAACCGCTACCTTTCACAGGGAGGCTCACATTCAAGGAAGATCAAAATGCCGTTAGCCGAATGCCACC  >  NC_002947/5903019‑5903311
                                                                                                                                                  |                                                                                                                                                    
cGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTG‑CCTGCGGGGGCCTCTTCGCGGGCGAGc                                                                                                                                                   <  2:333101/149‑1 (MQ=255)
 ggtcGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCATGGTTCGATAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTG‑CCTGCGGGGGCCTCTTCGCGGGCGAGcc                                                                                                                                                  <  5:23607/146‑1 (MQ=255)
     gCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGTGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTG‑CCTGCGGGGGCCTCTTCGCGGGCGAGCCcgcg                                                                                                                                              <  4:176333/149‑1 (MQ=255)
       atcatcGCCTTGCGGTCTCCTTGCTTGTGCTCAAATGATTTGTGGTATGCGACTGTGATGGTAGTTCAGGGTTCGATAAACATCTCTGACGGGGGCCATGCTGGCATTTTTGGAG‑CCTGCGGGGGCCTCTTCGCGGGCTCGCCCGCGaa                                                                                                                                            <  4:55812/149‑1 (MQ=255)
       aTCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGAGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTG‑CCTGCGGGGGCCCAATATCGGGCCAGCCcgcgc                                                                                                                                             >  2:155588/1‑147 (MQ=255)
        tCAACCCCATGCTGGCGCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGTGTTCGATAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTG‑CCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAag                                                                                                                                           <  7:244078/149‑1 (MQ=255)
          aaCGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTG‑CCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAagag                                                                                                                                         <  2:83955/149‑1 (MQ=255)
            cGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTG‑CCTGCGGGGGCCTCTTAGCGGGCGCGCCCGCGAAGAGGc                                                                                                                                       >  3:65142/1‑149 (MQ=255)
             gCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTG‑CCTGCGGGGGCCAAATAGCGGGCGACCCCGCGAAGAGGc                                                                                                                                       >  3:167175/1‑148 (MQ=255)
               cATGCTGGCTCCTTGTTTGTGCAAAATTGATTTGGGGCATGGGACTGTCTGCGTAGTTAAGGGTTCGAAAAACATCACTGACGGGGGCCTTGCTGGCATTTTTGGGG‑CCTGGGGGGGCATCTTGGGGGGCGTGCCCGGGATGAGGCCTc                                                                                                                                    <  2:209175/149‑1 (MQ=255)
                   cTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTG‑CCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCca                                                                                                                                  >  5:169690/1‑147 (MQ=255)
                          cttgcttgTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTG‑CCTGCGGGGGCCTCTTAGCGGGCGCGCCCGCGAAGAGGGCGCCACAGCCATGt                                                                                                                         >  4:354977/1‑149 (MQ=255)
                             gtttgTGCACAAATTTTTTGTGGGATGCGACTGTGAGCGTAGTTCAGTTTTCGTTAAACATCACTGCGGGGGGCCTTGCTGGCATTTTTGGTG‑CCTGCGGGGGCCTCTGCGCGGGCGAGCCCGCTAAGAGGCCTCCACAGCCATGTAt                                                                                                                       <  8:256469/146‑1 (MQ=255)
                                        aaTGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTG‑CCTGCGGGGGCCTCTTCGCGGGCTCGCCCGCGa                                                                                                                                             >  4:134558/1‑115 (MQ=255)
                                        aaTGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTG‑CCTGCGGGGGCCTCTTCGCGGGCTCGCCCGCGa                                                                                                                                             <  3:134558/115‑1 (MQ=255)
                                            aTTTGGGGGAGGCGCCTGTGATCGTAGTTCAGGGTTCGATAAACATCTCTGACGGGGGCCTTGCTGGCATTTTTGGTG‑CCTGCGGGGGCCTCTTCGCGGGCGAGCCCTCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATc                                                                                                       <  3:337262/149‑1 (MQ=255)
                                             tttGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTG‑CCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATc                                                                                                       <  8:353570/148‑1 (MQ=255)
                                                 tgGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTG‑CCTGCGGGGGCCTCATCACGGGCGGGCCCGCCACAAGGCCGGCACCACCAAGTTTAAGTATATGGCaaataccga                                                                                                   >  4:173503/1‑143 (MQ=255)
                                                   gtattctaCTGTGAGCGTAGTTCAGGGTTCTATAAACATCACTGACGGGGGCCATGCTGGAATTTTTGGTG‑CCTGCGGGGGCCTCTTCGCGGGCGAGCCGGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAAc                                                                                                <  4:65142/142‑1 (MQ=255)
                                                   ggATGCGACTGTCCGGTTATTCCCGGGTTCCAAAACCATCACTG‑CGGGGGCCCTGGCGGCAATTTTGGGGCCCTGCGGGGCCCTCTCCGCGGGCGCCCCCGCTAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGaaa                                                                                                 <  6:35702/148‑1 (MQ=255)
                                                            tgtgAGCGTAGTTCAGGGTTCGATAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTG‑CCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAagag                                                                                                                                         >  5:383475/1‑99 (MQ=255)
                                                            tgtgAGCGTAGTTCAGGGTTCGATAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTG‑CCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAagag                                                                                                                                         <  6:383475/99‑1 (MQ=255)
                                                                  cGTAGTTCAGGGTTCGATAAACTTCACTGACGGGGGCCATGCTGGCATTTTTGGTG‑CCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGcc                                                                                 <  3:354977/149‑1 (MQ=255)
                                                                  cGTAGTTCAGGGTTCGATAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTG‑CCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAg                                                                                                    <  4:288985/130‑1 (MQ=255)
                                                                  cGTAGTTCAGGGTTCGATAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTG‑CCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAg                                                                                                    >  3:288985/1‑130 (MQ=255)
                                                                                          cACTGACGGGGGCCATGCTGGATTTTTTGGTG‑CCTGGGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCAT‑CCCCGTTCCAACCGCTACCttt                                                        <  4:167175/149‑1 (MQ=255)
                                                                                            cTGACGGGGGCCATGCTGGCATTTTTGGTG‑CCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCAT‑CCCCGTTCCAACCGCTACCTTTca                                                      <  6:331913/149‑1 (MQ=255)
                                                                                             tGACGGGGGCCATGCTGGCATTTTTGGTG‑CCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCAT‑CCCCGTTCCAAc                                                                  <  7:99697/136‑1 (MQ=255)
                                                                                               ccGGGGGCCATGCTGGCATTTTTGGTG‑CCTGCGGGGGCCTCTTCGTGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCAT‑CCCCGTTCCAACCGCTACCTTTcaca                                                    <  8:325482/147‑1 (MQ=255)
                                                                                                    ggCCATGCTGGCATTTTTGGTG‑CCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCAT‑CCCCGTTCCAACCGCTACCTTTCACAGGGAgg                                              <  6:169690/149‑1 (MQ=255)
                                                                                                            tGGCATTTTTGGTG‑CCTGCGGGGGCCTATTAGCGGGCGAGCCCGCGAAGAGGGCTACACAGCCAAGTATCAGTATATGCCATATGCAGAAACCATCAGGCCTCGGCCAT‑CCCCGTTGCCACCGCGACCATTCACACGGAGGCGCACAt                                       >  2:280560/1‑148 (MQ=255)
                                                                                                             ggCATTTTTGGTG‑CCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGGAGCGGCCGCGACAGCCATGTATCAGTATCTGCCAGATCCCGGAACCATCAGGCCTCGGCCAT‑CCCCGTTCCCACCGCTACCGTTGACCGGGAGGCTcacagt                                      >  8:269017/1‑146 (MQ=255)
                                                                                                               cATTTTTGGTG‑CCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCAT‑CCCCGTTCCAACCGCTACCTTTCACAGGGAGGCTCACATTCaa                                   <  5:129320/149‑1 (MQ=255)
                                                                                                                aTTTTTGGTG‑CCTGCGGGGGCCGAATAGCGGGCGGGCCCCCGAAGAGGGCGCCCCACCCAAGTTTAAATATATGGCAAATACAGAAAACCACAGGCATAGGCCCTACCCCGTACC‑ACCCCGAACGTTGACAAGGGGGCGCACATTGAAg                                  >  4:17897/1‑149 (MQ=255)
                                                                                                                           gcTGTGGAGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAg                                                                                          <  7:345739/83‑1 (MQ=255)
                                                                                                                           gcTGTGGAGGCCTCTTAGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGGCATATACAGAAAACATCAg                                                                                          >  8:345739/2‑84 (MQ=37)
                                                                                                                                      ctctTCGCGGGCTCGCCCGCGAAGAGGCCCCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCAT‑CCCCGTTCCAACCGCTACCTTTCACAGGGAGGCTCACATTCAAGGAAGATCAAAATGCCGTTAGc             >  5:326716/1‑149 (MQ=255)
                                                                                                                                        ctTCGCGGGCTCGCCCGCGAAGAGGCCCCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCAT‑CCCCGTTCCAACCGCTACCTTTCACAGGGAGGCTCCCATTCAAGGAAGATAAAAATGCCGGTAGcc            >  7:166631/1‑148 (MQ=255)
                                                                                                                                           cgcgGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCAT‑CCCCGTTCCAACCGCTACCTTTCACAGGGAGGCTCACATTCAAGGAAGATCAAAATGCCGt                 >  7:137271/1‑140 (MQ=255)
                                                                                                                                           cgcgGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCAT‑CCCCGTTCCAACCGCTACCTTTCACAGGGAGGCTCACATTCAAGGAAGATCAAAATGCCGt                 <  8:137271/140‑1 (MQ=255)
                                                                                                                                                  gAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCAT‑CCCCGTTCCAACCGCTACCTTTCACAGGGAGGCTCACATTCAAGGAAGATCAAAATGCCGTTAGCCGAATGCCAcc  >  6:149050/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTG‑CCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCAT‑CCCCGTTCCAACCGCTACCTTTCACAGGGAGGCTCACATTCAAGGAAGATCAAAATGCCGTTAGCCGAATGCCACC  >  NC_002947/5903019‑5903311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑