Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,947,318 G→C 5.4% L386V (CTG→GTG)  rho ← transcription termination factor Rho

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,947,3180GC 5.4% 306.5 / 5.6 92L386V (CTG→GTG) rhotranscription termination factor Rho
Reads supporting (aligned to +/- strand):  ref base G (38/49);  new base C (2/3);  total (40/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.04e-01

GCCGCAAGGCGGCCCCAATCAATCCAGCCTGAAACGCTCGCTTACTTGCGCTTCATCGACAGGAAGAACTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCGGAGCGGTTGATGTTGATGGCCGGGAACACACGCTTCTCAGCGATGCGGCGGTCCAGCGGCAGCTCCATGTTGCCGGTAC  >  NC_002947/5947173‑5947464
                                                                                                                                                 |                                                                                                                                                  
gCCGCAAGGCGGCCCCAATCAATCCAGCCTGAAACGCTCGCTTACTTGCGCTTCATCGACAGGAAGAACTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCtt                                                                                                                                                 >  2:237290/1‑149 (MQ=255)
   gCAAGGCGGCCCCAATCAATCCAGCCTGAAACGCTCGCTTACTTGCGCTTCATCGACAGGAAGAACTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTgcg                                                                                                                                              <  3:242909/149‑1 (MQ=255)
    gaaGGCGTCCCCAATCAATCCAGCCTGAAACGCTCGCTTACTTGCGCTTCATCGACAGGATGAACTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTgcgc                                                                                                                                             <  7:256712/148‑1 (MQ=255)
      aGGCGGCCCCAATCAATCCAGCCTGAAACGCTCGCTTACTTGCGCTTCATCGACAGGAAGAACTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAg                                                                                                                                           <  7:204408/149‑1 (MQ=255)
        gcggcCCCAATCAATCCAGCCTGAAACGCTCGCTTACTTGCGCTTCATCGACAGGAAGAACTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGaa                                                                                                                                         <  6:101615/149‑1 (MQ=255)
          ggcCCCAATCAATCCAGCCTGAAACGCTCGCTTACTTGCGCTTCATCGACAGGAAGAACTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATc                                                                                                                                       <  5:144811/149‑1 (MQ=255)
          ggcCCCAATCAATCCAGCCTGAAACGCTCGCTTACTTGCGCTTCATCGACAGGAAGAACTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATc                                                                                                                                       >  4:262296/1‑149 (MQ=255)
             cccAATCAATCCAGCCTGAAACGCTCGCTTACTTGCGCTTCATCGACAGGAAGAACTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCcac                                                                                                                                    <  3:154166/149‑1 (MQ=255)
             cccAATCAATCCAGCCTGAAACGCTCGCTTACTTGCGCTTCATCGACAGGAAGAACTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCca                                                                                                                                     >  5:105906/1‑148 (MQ=255)
             cccAATCAATCCAGCCTGAAACGCTCGCTTACTTGCGCTTCATCGACAGGAAGAACTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCca                                                                                                                                     >  8:29168/1‑148 (MQ=255)
              ccAATCAATCCAGCCTGAAACGCTCGCTTACTTGCGCTTCATCGACAGGAAGAACTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCcaca                                                                                                                                   >  4:134857/1‑149 (MQ=255)
               caatcaatCCAGCCTGAAACGCTCGCTTACTTGCGCTTCATCGACAGGAAGAACTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACAt                                                                                                                                  <  3:5031/149‑1 (MQ=255)
                 atcaatcCAGCCTGAAACGCTCGCTTACTTGCTCTTCATCGACAGGAAGAACTCAACGTGGGTCTTGGTCTGCTTGAGCTTCTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGGAGCAGCTTGCGCAGAATCCAGATAc                                                                                                                                <  6:265783/149‑1 (MQ=255)
                 atcaatcCAGCCTGAAACGCTCGCTTACTTGCGCTTCATCGACAGGAAGAACTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACAt                                                                                                                                  >  6:13025/1‑147 (MQ=255)
                 atcaatcCAGCCTGAAACGCTCGCTTACTTGCGCTTCATCGACAGGAAGAACTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACAt                                                                                                                                  <  5:13025/147‑1 (MQ=255)
                  tcaatcCAGCCTGAAACGCTCGCTTACTTGCGCTTCATCGACAGGAAGAACTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACg                                                                                                                               <  6:105906/149‑1 (MQ=255)
                    aatcCAGCCTGAAACGCTCGCTTACTTGCGCTTCATCGACAGGAAGAACTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGc                                                                                                                              <  2:96658/148‑1 (MQ=255)
                     atcCAGCCTGAAACGCTCGCTTACTTGCGCTTCATCGACAGGAAGAACTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGTTGCAGCAGCTTGCGCAGAAGCCACATACGCTg                                                                                                                            >  2:316978/1‑149 (MQ=255)
                        cAGCCTGAAACGCTCGCTTACTTGCGCTTCATCGACAGGAAGAACTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTAGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAg                                                                                                                         >  6:188913/1‑149 (MQ=255)
                         aGCCTGAAACGCTCGCTTACTTGCGCTTCATCGACAGGAAGAACTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCACCTTGCGCAGAATCCACATACGCTGCAGc                                                                                                                        <  4:127265/149‑1 (MQ=255)
                           ccTGAAACGCTCGCTTACTTGCGCTTCATCGACAGGAAGAACTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTg                                                                                                                            >  2:230114/1‑143 (MQ=255)
                           ccTGAAACGCTCGCTTACTTGCGCTTCATCGACAGGAAGAACTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTg                                                                                                                            <  1:230114/143‑1 (MQ=255)
                            cTGAAACGCTCGCTTACTTGCGCTTCATCGACAGGAAGAACTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCa                                                                                                                                            <  7:89880/126‑1 (MQ=255)
                            cTGAAACGCTCGCTTACTTGCGCTTCATCGACAGGAAGAACTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCa                                                                                                                                            >  8:89880/1‑126 (MQ=255)
                               aaaCGCTCGCTTACTTGCGCTTCATCGACAGGAAGAACTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCtcgtc                                                                                                                   <  5:93010/148‑1 (MQ=255)
                               aaaCGCTCGCTTACTTGCGCTTCATCGACAGGAAGAACTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCtcgtc                                                                                                                   <  7:292298/148‑1 (MQ=255)
                                 aCGCTCGCTTACTTGCGCTTCATCGACAGGAAGAACTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTcgtcgt                                                                                                                 >  3:215329/1‑148 (MQ=255)
                                     tcgctTACTTGCGCTTCATCGACAGGAAGAACTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCg                                                                                                            >  2:162506/1‑149 (MQ=255)
                                       gctTACTTGCGCTTCATCGACAGGAAGAACTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGt                                                                                                          <  1:301113/149‑1 (MQ=255)
                                           aCTTGCGCTTCATCGACAGGAAGAACTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCa                                                                                                        <  6:138026/147‑1 (MQ=255)
                                           aCTTGCGCTTCATCGACAGGAAGAACTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAg                                                                                                       <  6:244287/148‑1 (MQ=255)
                                           aCTTGCGCTTCATCGACAGGAAGAACTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAg                                                                                                       <  4:60196/148‑1 (MQ=255)
                                            cTTGCGCTTCATCGACAGGAAGAACTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGc                                                                                                      <  3:134857/148‑1 (MQ=255)
                                            cTTGCGCTTCATCGACAGGAAGAACTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGc                                                                                                      <  4:81323/148‑1 (MQ=255)
                                             ttGCGCTTCATCGACAGGAAGAACTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCaa                                                                                                    >  4:319515/1‑149 (MQ=255)
                                                cgcTTCATCGACAGGAAGAACTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCACCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAActc                                                                                                 >  7:60247/1‑149 (MQ=255)
                                                      aTCGACAGGAAGAACTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTcgcg                                                                                           <  4:258814/149‑1 (MQ=255)
                                                      aTCGACAGGAAGAACTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTcgc                                                                                            <  4:215329/148‑1 (MQ=255)
                                                         gACAGGAAGAACTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCgcggc                                                                                         <  6:75029/148‑1 (MQ=255)
                                                         gACAGGAAGAACTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCgcggc                                                                                         >  5:9909/1‑148 (MQ=255)
                                                            aGGAATAACTCCTCGTTGGTCGTGGTCTGCTTGAGCTTGTCGCCCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCTCATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGt                                                                                     <  6:283742/149‑1 (MQ=255)
                                                              gaagaaCTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGa                                                                                       >  4:227412/1‑145 (MQ=255)
                                                              gaagaaCTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGa                                                                                       <  3:227412/145‑1 (MQ=255)
                                                              gaagaaCTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGc                                                                                   <  6:94158/149‑1 (MQ=255)
                                                              gaagaaCTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGc                                                                                   >  8:253029/1‑149 (MQ=255)
                                                                agaaCTCATCGTTGGTCTTGGTCTGCTTGAGCGTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCg                                                                                 <  5:35439/149‑1 (MQ=255)
                                                                    cTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCGGAGc                                                                             >  8:727/1‑149 (MQ=255)
                                                                     tCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCGGAGCg                                                                            >  2:25497/1‑149 (MQ=255)
                                                                          gTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCa                                                                                                        >  4:30503/1‑116 (MQ=255)
                                                                          gTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCa                                                                                                        <  3:30503/116‑1 (MQ=255)
                                                                          gTTGGTCATGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGCCGAGTGCCGGAGCGgttga                                                                       <  2:182640/149‑1 (MQ=255)
                                                                          cttggtcttgggcTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCGGAGCGgttg                                                                        <  2:326482/147‑1 (MQ=255)
                                                                           ttggtcttggtcTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCGGAGCGgttgat                                                                      <  1:275657/149‑1 (MQ=255)
                                                                             ggtcttggtctGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCGGAGCGgttgatg                                                                     <  1:365446/148‑1 (MQ=255)
                                                                             ggtcttggtctGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCGGAGCGgttgatg                                                                     <  3:370017/148‑1 (MQ=255)
                                                                              gtcttggtctGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCGGAGCGgttgatgt                                                                    <  1:162506/148‑1 (MQ=255)
                                                                               tcttggtctGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCGGAGCGgttgatgtt                                                                   >  1:168398/1‑148 (MQ=255)
                                                                               tcttggtctGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCACCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCGGAGCGgttgatgttg                                                                  <  8:60247/149‑1 (MQ=255)
                                                                                         cTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCGGAGCGgt                                                                          <  6:298085/131‑1 (MQ=255)
                                                                                         cTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCGGAGCGGTTGATGTTGATGGCCggg                                                         >  7:320260/1‑148 (MQ=255)
                                                                                             aGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCGGAGCGGTTGATGTTGATGGCCGGGAaca                                                     <  7:727/148‑1 (MQ=255)
                                                                                              gCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCGGAGCGGTTGATGTTGATGGCCGGGAacaca                                                   >  5:70836/1‑149 (MQ=255)
                                                                                                   tCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCGGAGCGGTTGATGTTGATGGCCGGGAACACACGCt                                               >  7:122642/1‑148 (MQ=255)
                                                                                                   tCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCGGAGCGGTTGATGTTGATGGCCGGGAACACACGCt                                               >  3:197997/1‑148 (MQ=255)
                                                                                                     gACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCGGAGCGGTTGATGTTGATGGCCGGGAACACACGCTtc                                             >  3:316021/1‑148 (MQ=255)
                                                                                                        cAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGGAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCGGAGCGGTTGATGTTGATGGCCGGGAACACACGCTTCTCAg                                         >  7:123109/1‑149 (MQ=255)
                                                                                                          ggAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCGGAGCGGTTGATGTTGATGGCCGGGAACACACGCTTCTCAGc                                        >  3:215515/1‑148 (MQ=255)
                                                                                                           gAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCGGAGCGGTTGATGTTGATGGCCGGGAACACACGCTTCTCAGCGa                                      >  6:321366/1‑149 (MQ=255)
                                                                                                           gAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCGGAGCGGTTGATGTTGATGGCCGGGAACACACGCTTCTCAGCGa                                      >  8:354203/1‑149 (MQ=255)
                                                                                                            aaCTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCGGAGCGGTTGATGTTGATGGCCGGGAACACACGCTTCTCAGCGAt                                     <  8:320260/149‑1 (MQ=255)
                                                                                                            aaCTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCGGAGCGGTTGATGTTGATGGCCGGGAACACACGCTTCTCAGCGAt                                     <  5:321366/149‑1 (MQ=255)
                                                                                                                cGATGGCAGCGAGTTCGTCCATCGGGTGCAGGAGCTTCCTCAGACTCCACATACGCTGCAGCTCGTCTTCAGCGGTCAGCAACTCTTCGCTGCGAGTGCCGGAGCGGTTGATGTTGATGTCCGGGAACACACGCTTCTCAGCTATgcgt                                 <  8:304048/149‑2 (MQ=255)
                                                                                                                 gATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCGGAGCGGTTGATGTTGATGGCCGGGAACACACGCTTCTCAGCGATgcggc                                >  8:294942/1‑149 (MQ=255)
                                                                                                                 gATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCGGAGCGGTTGATGTTGATGGCCGGGAACACACGCTTCTCAGCGATgcgg                                 <  8:122642/148‑1 (MQ=255)
                                                                                                                 gATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCGGAGCGGTTGATGTTGATGGCCGGGAACACACGCTTCTCAGCGATgcgg                                 <  7:29168/148‑1 (MQ=255)
                                                                                                                  aTGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCGGAGCGGTTGATGTTGATGGCCGGGAACACACGCTTCTCAGCGATgcggcg                               >  6:170420/1‑149 (MQ=255)
                                                                                                                   tGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCGGAGCGGTTGATGTTGATGGCCGGGAACACACGCTTCTCAGCGATGcggcgg                              <  3:319515/149‑1 (MQ=255)
                                                                                                                   tGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTAGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCGGAGCGGTTGATGTTGATGGCCGGGAACACACGCTTCTCAGCGATGcggcgg                              >  3:351352/1‑149 (MQ=255)
                                                                                                                      cAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCGGAGCGGTTGATGTTGATGGCCGGGAACACACGCTTCTCAGCGATGCGGCGGTc                            >  8:161337/1‑148 (MQ=255)
                                                                                                                       aGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCGGAGCGGTTGATGTTGATGGCCGGGAACACACGCTTCTCAGCGATGCGGCGGTCCa                          <  7:161337/149‑1 (MQ=255)
                                                                                                                       aGCGATTTCGTCCATCGGGTGCAGCACCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCGGAGCGgtt                                                                         <  1:301398/102‑1 (MQ=255)
                                                                                                                       aGCGATTTCGTCCATCGGGTGCAGCACCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCGGAGCGgtt                                                                         >  2:301398/1‑102 (MQ=255)
                                                                                                                             ttCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCGGAGCGGTTGATGTTGATGGCCGGGAACACACGCTTCTCAGCGATGCGGCGGTCCAGCgg                      >  1:188321/1‑147 (MQ=255)
                                                                                                                               cGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGATGCAGCTCGTCCTCAGCGGTAAGCATCTCTTCGCGGCGAGTGCCGGAGCGGTTGATGTTGATGGACGGGAACACACGCATCTCAGCGATGCGGCGGTCCAGCGGCAGc                  >  8:165404/1‑149 (MQ=255)
                                                                                                                                gTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCGGAGCGGTTGATGTTGATGGCCGGGAACACACGCTTCTCAGCGATGCGGCGGTCCAGCGGCAGCt                 >  5:134808/1‑149 (MQ=255)
                                                                                                                                gTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCGGAGCGGTTGATGTTGATGGCCGGGAACACACGCTTCTCAGCGATGCGGCGGTCCAGCGGCAGCt                 >  5:187861/1‑149 (MQ=255)
                                                                                                                                   cATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCGGAGCGGTTGATGTTGATGGCCGGGAACACACGCTTCTCAGCGATGCGGCGGTCCAGCGGCAGCTcc               <  3:262296/148‑1 (MQ=255)
                                                                                                                                       gggTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCGGAGCGGTTGATGTTGATGGCCGGGAACACACGCTTCTCAGCGATGCGGCGGTCCAGCGGCAGCTCCATGtt          >  4:302135/1‑149 (MQ=255)
                                                                                                                                         gTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCGGAGCGGTTGATGTTGATGGCCGGGAACACACGCTTCTCAGCGATGCGGCGGTCCAGCGGCAGCTCCATGTTGc        <  4:215515/149‑1 (MQ=255)
                                                                                                                                         gTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCGGAGCGGTTGATGTTGATGGCCGGGAACACACGCTTCTCAGCGATGCGGCGGTCCAGCGGCAGCTCCATGTTGc        <  6:9909/149‑1 (MQ=255)
                                                                                                                                         gTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCGGAGCGGTTGATGTTGATGGCCGGGAACACACGCTTCTCAGCGATGCGGCGGTCCAGCGGCAGCTCCATGTTGc        <  4:316021/149‑1 (MQ=255)
                                                                                                                                          tGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGGCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCGGAGCGGTGGATGTTGATGGCCGGGAACACACGCTTCTCAGCGATGCGGCGGTCCAGCGGCAGCTCCATGTTGcc       <  6:140505/149‑1 (MQ=255)
                                                                                                                                            cagcagCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCGGAGCGGTTGATGTTGATGGCCGGGAACACACGCTTCTCAGCGATGCGGCGGTCCAGCGGCAGCTCCATGTTGCCg      <  6:187861/148‑1 (MQ=255)
                                                                                                                                              gcagcTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCGGAGCGGTTGATGTTGATGGCCGGGAACACACGCTTCTCAGCGATGCGGCGGTCCAGCGGCAGCTCCATGTTGCCGGTa   >  7:253462/1‑149 (MQ=255)
                                                                                                                                               cagcTTGCGCAGAATCAACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCACGAGCGGTTGATGTTGATGGCCGGGAACACACGCTACTCAGCGATGCGGCGCTCCCGCGGCACATCCATCTTGCCGGAAc  >  4:108332/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GCCGCAAGGCGGCCCCAATCAATCCAGCCTGAAACGCTCGCTTACTTGCGCTTCATCGACAGGAAGAACTCATCGTTGGTCTTGGTCTGCTTGAGCTTGTCGACCAGGAACTCGATGGCAGCGATTTCGTCCATCGGGTGCAGCAGCTTGCGCAGAATCCACATACGCTGCAGCTCGTCGTCAGCGGTCAGCAACTCTTCGCGGCGAGTGCCGGAGCGGTTGATGTTGATGGCCGGGAACACACGCTTCTCAGCGATGCGGCGGTCCAGCGGCAGCTCCATGTTGCCGGTAC  >  NC_002947/5947173‑5947464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑