BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,959,995	A→T	5.0%	noncoding (74/103 nt)	PP_mr63 ←	rnk_pseudo ncRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,959,995	0	A	T	5.0%	350.4 / 2.1	115	noncoding (74/103 nt)	PP_mr63	rnk_pseudo ncRNA
Reads supporting (aligned to +/- strand): ref base A (61/48); new base T (4/2); total (65/50)
Fisher's exact test for biased strand distribution p-value = 6.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

GTACTCTCGTCGAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCGGCGCCGTTCG  >  NC_002947/5959850‑5960142

gTACTCTCGTCGAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAgg                                                                                                                                                   <  3:81058/149‑1 (MQ=255)

    tctcGTCGAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCgg                                                                                                                                               >  2:80705/1‑149 (MQ=255)

    tctcGTCGAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCACAAGGGCgg                                                                                                                                               >  6:32566/1‑149 (MQ=255)

      tcgtcgAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCATACTCCTTTGAACAGGCATTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGTCgggg                                                                                                                                             >  4:25248/1‑149 (MQ=255)

        gtcgAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGATGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGtt                                                                                                                                           <  6:7222/149‑1 (MQ=255)

        gtcgAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGtt                                                                                                                                           >  5:132689/1‑149 (MQ=255)

         ccgAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGACGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGttt                                                                                                                                          <  3:281881/148‑1 (MQ=255)

           gAGGCTGTCGATCAGACGCTCCAGCCGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTga                                                                                                                                        <  1:336519/149‑1 (MQ=255)

           gAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGttt                                                                                                                                          >  4:297099/1‑147 (MQ=255)

             ggCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGATGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTgaga                                                                                                                                      <  3:317891/149‑1 (MQ=255)

             ggCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTgag                                                                                                                                       >  2:206907/1‑148 (MQ=255)

              gCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTgag                                                                                                                                       >  3:98370/1‑147 (MQ=255)

                         gACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCTTGGGCGGGGttttgct                                                                                                                                      <  2:356344/137‑5 (MQ=255)

                         gACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCTTGGGCGGGGttttgct                                                                                                                                      >  1:356344/1‑133 (MQ=255)

                         gACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑Gt                                                                                                                         >  8:117974/1‑149 (MQ=255)

                         gACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑Gt                                                                                                                         >  1:230493/1‑149 (MQ=255)

                          aCGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGaa                                                                                                                                     <  5:139611/137‑1 (MQ=255)

                          aCGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGaa                                                                                                                                     >  6:139611/1‑137 (MQ=255)

                              tCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCAcc                                                                                                                     >  8:84966/1‑148 (MQ=255)

                                 aGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACg                                                                                                                 >  7:311745/1‑149 (MQ=255)

                                   aCGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAATCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCa                                                                                                               <  8:304658/149‑1 (MQ=255)

                                   aCGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCa                                                                                                               <  6:170469/149‑1 (MQ=255)

                                     gCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACACCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAg                                                                                                              >  7:142329/1‑148 (MQ=255)

                                      cTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGaa                                                                                                            <  8:41259/149‑1 (MQ=255)

                                         tACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAAccc                                                                                                         <  2:70029/149‑1 (MQ=255)

                                         tACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAAccc                                                                                                         >  1:221206/1‑149 (MQ=255)

                                           cGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGa                                                                                                       <  4:2532/149‑1 (MQ=255)

                                            gTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGa                                                                                                       >  1:155298/1‑148 (MQ=255)

                                             tCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGaca                                                                                                     >  7:243870/1‑149 (MQ=255)

                                             tCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGaca                                                                                                     >  1:128849/1‑149 (MQ=255)

                                               cAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGgcac                                                                                                    >  8:57679/1‑148 (MQ=255)

                                               cAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACt                                                                                                   <  1:198726/149‑1 (MQ=255)

                                                    gggTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACcac                                                                                              <  2:63102/149‑1 (MQ=255)

                                                    gggTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACca                                                                                               <  4:297570/148‑1 (MQ=255)

                                                          ggATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGGGGGGTTTGAGATGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCg                                                                                         <  1:250616/148‑1 (MQ=255)

                                                          ggATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCgg                                                                                        <  2:230493/149‑1 (MQ=255)

                                                            aTGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGc                                                                                      <  8:262044/149‑1 (MQ=255)

                                                               aGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGTCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCaaa                                                                                   <  5:169222/149‑1 (MQ=255)

                                                                gggAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCaaa                                                                                   >  5:243542/1‑148 (MQ=255)

                                                                 ggAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATa                                                                                 <  6:243542/149‑1 (MQ=255)

                                                                    aGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTc                                                                              <  6:94070/149‑1 (MQ=255)

                                                                         tGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGc                                                                          <  2:155298/148‑1 (MQ=255)

                                                                            tGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACTCAGAACCCGACACTACCACAGACGGGCAAATACTCAAGCCCCt                                                                      >  3:241528/1‑149 (MQ=255)

                                                                                 aTGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACg                                                                                                                 <  1:239222/101‑1 (MQ=255)

                                                                                  tGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCt                                                                 >  1:102566/1‑148 (MQ=255)

                                                                                    aTCCAGTCAGACTCCTTTGAACAGGAAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCa                                                               <  3:25248/148‑1 (MQ=255)

                                                                                     tCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAgt                                                             <  3:297099/149‑1 (MQ=255)

                                                                                     tCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAgt                                                             >  7:273519/1‑149 (MQ=255)

                                                                                     tCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCACCTGCGCTCAgt                                                             >  6:166495/1‑149 (MQ=255)

                                                                                     tCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGATTTGGC‑GTCACCGAAGCAGAACCCGACACTACCACAGCCGGGCAAATACTAAAGCCCCTGCGCTCAgt                                                             >  7:268507/1‑149 (MQ=255)

                                                                                        aGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGtgtg                                                          >  6:186095/1‑149 (MQ=255)

                                                                                        aGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGtgtg                                                          >  1:114943/1‑149 (MQ=255)

                                                                                             gACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTa                                                     <  7:117974/149‑1 (MQ=255)

                                                                                             gACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTa                                                     <  1:80705/149‑1 (MQ=255)

                                                                                             gACTCCTTTCAACAGTCAGTTTTCAATGTGCAAATAAAGCAAAACCCCCCCCATGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTa                                                     <  8:243870/149‑1 (MQ=255)

                                                                                              aCTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGC‑AATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGc                                                   <  2:128849/149‑1 (MQ=255)

                                                                                                tCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGcc                                                  >  8:171210/1‑149 (MQ=255)

                                                                                                  cTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTg                                                >  6:262554/1‑149 (MQ=255)

                                                                                                  cTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTg                                                >  5:311677/1‑149 (MQ=255)

                                                                                                   tttGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAAACCCGCCCAAGGGCGGGGTTTGAGATGGCTTTGGC‑GTCACAGACGCAGAACCCGACACTACCACAGCCGGGAAAATACTCAAGACCCTGCGCTCAGTGTGCCTTAGCCTGc                                               >  8:330395/1‑149 (MQ=255)

                                                                                                       aaCAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTcgc                                           >  3:28458/1‑149 (MQ=255)

                                                                                                       aaCAGGCAGATTTCAATGTGAAAATAAAGCAAAAACCCGCCCAAGGGCGGGGTTTCAGAAGTCATTGCC‑GTCACCGACGCAGAACCCGACACTAACACACACGGGCAAAAACTCAAGCCCCACCGCACAGATTGCCTAAGCCtcatc                                             >  2:218317/1‑143 (MQ=255)

                                                                                                         cAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCgcgc                                         <  6:294853/149‑1 (MQ=255)

                                                                                                            gCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGTC‑GTCACCGAAGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCACTGCGCTCAGTGTGCCTTAGCCTGCTCgcgc                                         >  5:79149/1‑146 (MQ=255)

                                                                                                            gCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTGCCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCgcgc                                         >  5:254546/1‑146 (MQ=255)

                                                                                                            gCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCgcgc                                         >  8:192791/1‑146 (MQ=255)

                                                                                                             cAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGa                                     <  3:295618/149‑1 (MQ=255)

                                                                                                                ttttCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTa                                                     >  6:372196/1‑130 (MQ=255)

                                                                                                                     aaTGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGc                              <  2:114943/148‑1 (MQ=255)

                                                                                                                     aaTGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGc                              <  7:171210/148‑1 (MQ=255)

                                                                                                                         tgCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTcgc                         >  6:104513/1‑149 (MQ=255)

                                                                                                                         tgCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTcgc                         <  7:84966/149‑1 (MQ=255)

                                                                                                                         tgCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTcgc                         >  2:163123/1‑149 (MQ=255)

                                                                                                                          gCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCATGCGCTCAGTGTGCCTTAGCATGGTCGCGCAAGACATGGGCATcgc                         >  1:93805/1‑149 (MQ=255)

                                                                                                                          gCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAACTACTCACGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACGTGGGCCTcgcg                        >  2:368752/1‑149 (MQ=255)

                                                                                                                             aaTAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCgg                       >  7:209145/1‑147 (MQ=255)

                                                                                                                             aaTAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCgg                       >  8:50154/1‑147 (MQ=255)

                                                                                                                              aTAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAg                    <  7:57679/149‑1 (MQ=255)

                                                                                                                                 aaGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATc                 >  2:204823/1‑149 (MQ=255)

                                                                                                                                  aGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCt                >  7:173256/1‑149 (MQ=255)

                                                                                                                                   gCAAAACCCCGCCCAAGGGGGGGGTTTGAGATGGCTTTGGC‑GTCACCGACGCAGCACCCGTCACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGGCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTg               >  6:109538/1‑149 (MQ=255)

                                                                                                                                    cAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGTGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGc              <  2:221206/149‑1 (MQ=255)

                                                                                                                                    cAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGc              >  5:244016/1‑149 (MQ=255)

                                                                                                                                     aaaaCCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGcc             >  3:327456/1‑149 (MQ=255)

                                                                                                                                      aaaCCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCGAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTggg                               <  1:110500/130‑1 (MQ=255)

                                                                                                                                      aaaCCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCGAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTggg                               >  2:110500/1‑130 (MQ=255)

                                                                                                                                      aaaCCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCg            <  5:104513/149‑1 (MQ=255)

                                                                                                                                       aaCCCCGCCCTTGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCGAGACCTGGGCCTCGCGGCAGATCTGCCgg           >  1:97003/1‑149 (MQ=255)

                                                                                                                                        aCCCCGCCCTTGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCGgc          >  5:228375/1‑149 (MQ=255)

                                                                                                                                         ccccGCCCTTGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTggg                               >  7:74356/1‑127 (MQ=255)

                                                                                                                                         ccccGCCCTTGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTggg                               <  8:74356/127‑1 (MQ=255)

                                                                                                                                          cccGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCgcgag        <  5:262554/149‑5 (MQ=255)

                                                                                                                                           ccGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCTGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCGgcgc        >  1:237118/1‑149 (MQ=255)

                                                                                                                                           ccGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCGGCGcc       >  4:119935/1‑149 (MQ=255)

                                                                                                                                            cGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTa                                                     >  1:151576/1‑102 (MQ=255)

                                                                                                                                             gCCCAATGGCTGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACATCCGGGCAAATTCTCAAGGCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCGGCGCCGt     <  7:330395/149‑1 (MQ=255)

                                                                                                                                             gCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCGGCGCCGt     <  8:173256/149‑1 (MQ=255)

                                                                                                                                              cccAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCGGAGCCGtt    >  4:239665/1‑149 (MQ=255)

                                                                                                                                               ccAAGGGGGGGGTTTGAGAAGGCATTGGC‑TTCACCGACGCATAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCGGCGCCGTTc   <  4:98370/149‑1 (MQ=255)

                                                                                                                                                cAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACAGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGAATTAGCCTGCTCGCGCAAGACCTGGGCCGCGCGGCAGAACTGCCGGCGCCGTTCg  >  4:290604/1‑149 (MQ=255)

GTACTCTCGTCGAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGC‑GTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCGGCGCCGTTCG  >  NC_002947/5959850‑5960142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`