| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,962,276 | G→T | 10.0% | G391V (GGC→GTC) | lysA‑II → | diaminopimelate decarboxylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,962,276 | 0 | G | T | 10.0% | 171.1 / 6.8 | 57 | G391V (GGC→GTC) | lysA‑II | diaminopimelate decarboxylase |
| Reads supporting (aligned to +/- strand): ref base G (22/29); new base T (2/4); total (24/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.68e-01 | |||||||||||
TGCGAGACTGGCGACTTCCTCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCATGGTCCTGGACCTGGTCAGCCA > NC_002947/5962128‑5962424 | tGCGAGACTGGCGACTTCCTCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACgg > 6:6927/1‑149 (MQ=255)tGCGAGACTGGCGACTTCCTCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACgg > 8:62304/1‑149 (MQ=255) cGAGACTGGCGAGTTCCTCGGCAAGGACCGCGTCCTGATCCTGGCCTAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCTCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGc < 7:164168/148‑1 (MQ=255) gagaCTGGCGACTTCCTCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGCCGTCGa > 4:224979/1‑149 (MQ=255) gCGACTTCCTCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATTGTTTTGTCATGAGCTCCAACTACAACACCCGCTGCCGTTGCGCTGAAATCCTGGTCGCCGTCGACCAggat < 2:266009/149‑3 (MQ=255) cTTCCTCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGCCGTCGACCAggatttca < 8:208668/149‑7 (MQ=255) cTTCCTCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGCCGTCGACCAggatttca < 3:224979/149‑7 (MQ=255) cTTCCTCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGCCGTCGACCAggatttca < 5:380546/149‑7 (MQ=255) ttCCTCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCg > 5:268630/1‑148 (MQ=255) ttCCTCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCg > 3:201368/1‑148 (MQ=255) tCCTCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCg > 3:141758/1‑147 (MQ=255) ccTCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGaa < 7:62304/148‑1 (MQ=255) tCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTa > 2:31388/1‑149 (MQ=255) ccTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCg > 5:62113/1‑149 (MQ=255) tCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTc > 1:156155/1‑149 (MQ=255) aaCCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGc < 6:27867/149‑1 (MQ=255) ccTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGAACGCCGCCGCGAGACCGTCGcc > 6:123890/1‑148 (MQ=255) gCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTTCGCCGCCGCGAGACCGTCGCCGAAc < 2:183923/148‑1 (MQ=255) gCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGaa < 8:187322/147‑1 (MQ=255) gCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACt < 4:336012/149‑1 (MQ=255) gCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACt < 1:189992/149‑1 (MQ=255) gAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTa > 8:236069/1‑149 (MQ=255) gTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCt < 3:24017/149‑1 (MQ=255) tGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTg < 1:31388/149‑1 (MQ=255) tGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGAACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTg < 5:123890/149‑1 (MQ=255) gACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTgg > 5:20044/1‑149 (MQ=255) ctgGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGaaa < 5:214881/148‑1 (MQ=255) cGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCctgc > 7:289879/1‑149 (MQ=255) cGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCctgc > 6:148325/1‑149 (MQ=255) ggcCTATGGTTTTGTCTTGAGCTCCAACTACAACACCGGCGGCGGTTGCGCTGAAATCGTGGTCGACGGCGACCAGGCGTTCGTAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGccc < 4:267243/148‑1 (MQ=255) cgcCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGccc < 2:82707/149‑1 (MQ=255) ccTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCAt < 5:180080/149‑1 (MQ=255) cTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCAtg > 3:355403/1‑149 (MQ=255) cTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCAtg < 5:148325/149‑1 (MQ=255) tATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCAtgc > 4:186558/1‑149 (MQ=255) gTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCAtgctgc < 4:141758/148‑1 (MQ=255) tCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGttt < 6:20044/149‑1 (MQ=255) tCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGtt < 2:156155/148‑1 (MQ=255) aTGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGTGTAAGCCCATGCTGCTGCGTTTTa < 7:236069/149‑1 (MQ=255) tGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTa > 8:143704/1‑100 (MQ=255) tGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTAc < 5:6927/149‑1 (MQ=255) ccAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGAt > 3:27456/1‑149 (MQ=255) cAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGGAAGCCCATGCTGGTGCGTTGTACCAAGATg > 8:286279/1‑149 (MQ=255) aCTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAAc > 3:210992/1‑87 (MQ=255) aCTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAAc < 4:210992/87‑1 (MQ=255) acaacaCCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCAtgg > 6:369659/1‑149 (MQ=255) caacaCCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCAtg < 4:27456/147‑1 (MQ=255) cccGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCAtgggctggg < 1:299442/149‑1 (MQ=255) ttGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCCAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTc < 8:194979/149‑1 (MQ=255) gcgcTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCa < 3:186558/148‑1 (MQ=255) tcgaacgccTGGTCGCCGTCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCATGGt > 1:350462/8‑149 (MQ=255) tGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTAc > 6:353378/1‑115 (MQ=255) tGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTAc < 5:353378/115‑1 (MQ=255) tCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCATGGTCCTGGa > 2:373420/1‑149 (MQ=255) gTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCATGGTCCTGGACCTg < 1:373420/148‑1 (MQ=255) gACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCATGGTCCTGGACCTGGt > 7:196828/1‑147 (MQ=255) aCGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCATgg > 3:213288/1‑133 (MQ=255) aCGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCATgg < 4:213288/133‑1 (MQ=255) cGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCATGGTCCTGGACCTGGTcagc < 6:62113/149‑1 (MQ=255) gCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCATGGTCCTGGACCTGGTcagcca < 4:201368/149‑1 (MQ=255) | TGCGAGACTGGCGACTTCCTCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCATGGTCCTGGACCTGGTCAGCCA > NC_002947/5962128‑5962424 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |