Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,982,808 C→G 5.7% N1151K (AAC→AAG)  PP_5242 → GAF domain‑containing sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,982,8080CG 5.7% 318.7 / 4.1 97N1151K (AAC→AAG) PP_5242GAF domain‑containing sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base C (45/46);  new base G (3/3);  total (48/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGCCGCACCGGCGCACATCAACCTGGCCGACTATCACCTGTTCCGCGGCCTGGCCCTGGGCAGCCCCGAGGCCCACGGCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAGGCGCAAATCGCCGCCACCGCTGCCGGCTTCATCCACGAGCAGGCGCTGGCCCATG  >  NC_002947/5982660‑5982956
                                                                                                                                                    |                                                                                                                                                    
gggCCGCACCGGCGCACATCAACCTGGCCGACTATCACCTGTTCCGCGGCCTGGCCCTGGGCAGCCCCGAGGCCCACGGCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAAc                                                                                                                                                      >  3:48476/1‑149 (MQ=255)
    cGCACCGGCGCACATCAACCTGGCCGACTATCACCTGTTCCGCGGCCTGGCCCTGGGCAGCCCCGAGGCCCACGGCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCgg                                                                                                                                                  <  4:333858/149‑1 (MQ=255)
      cACCGGCGCACATCAACCTGGCCGACTATCACCTGTTCCGCGGCCTGGCCCTGGGCAGCCCCGAGGCCCACGGCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGcc                                                                                                                                                >  3:89074/1‑149 (MQ=255)
       aCCGGCGCACATCAACCTGGCCGACTATCACCTGTTCCGCGGCCTGGCCCTGGGCAGCCCCGAGGCCCACGGCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCa                                                                                                                                               <  1:259713/149‑1 (MQ=255)
       aCCGGCGCACATCAACCTGGCCGACTATCACCTGTTCCGCGGCCTGGCCCTGGGCAGCCCCGAGGCCCACGGCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCa                                                                                                                                               <  4:85952/149‑1 (MQ=255)
         cGGCGCACATCAACCTGGCCGACTATCACCTGTTCCGCGGCCTGGCCCTGGGCAGCCCCGAGGCCCACGGCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGGTTGAAGCCGGcccag                                                                                                                                             <  7:79059/149‑4 (MQ=255)
          ggCGCACATCAACCTGGCCGACTATCACCTGTTCCGCGGCCTGGCCCTGGGCAGCCCCGAGGCCCACGGCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCt                                                                                                                                            >  6:242428/1‑149 (MQ=255)
           gcgcACATCAACCTGGCCGACTATCACCTGTTCCGCGGCCTGGCCCTGGGCAGCCCCGAGGCCCACGGCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGGTTGAAGCCGGcccagcg                                                                                                                                           <  7:67901/149‑6 (MQ=255)
              cacaTCAACCTGGCCGACTATCACCTGTTCCGCGGCCTGGCCCTGGGCAGCCCCGAGGCCCACGGCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCg                                                                                                                                        <  1:229409/149‑1 (MQ=255)
              cacaTCAACCTGGCCGACTATCACCTGTTCCGCGGCCTGGCCCTGGGCAGCCCCGAGGCCCACGGCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCg                                                                                                                                        <  4:48476/149‑1 (MQ=255)
               acaTCAACCTGGCCAACTATCACCTGTTCCGCGGCCTGGCCCTGGGCAGCCCCGAGGCCCACGGCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCg                                                                                                                                        >  3:27402/1‑148 (MQ=255)
                caTCAACCTGGCCGACTATCACCTGTTCCGCGGCCTGGCCCTGGGCAGCCCCGAGGCCCACGGCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCgca                                                                                                                                      <  5:229575/149‑1 (MQ=255)
                 aTCAACCTGGCCGACTATCACCTGTTCCGCGGCCTGGCCCTGGGCAGCCCCGAGGCCCACGGCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCgcag                                                                                                                                     >  1:53123/1‑149 (MQ=255)
                  tCAACCTGGCCGACTATCACCTGTTCCGCGGCCTGGCCCTGGGCAGCCCCGAGGCCCACGGCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCgcagc                                                                                                                                    <  6:289547/149‑1 (MQ=255)
                  tCAACCTGGCCGACTATCACCTGTTCCGCGGCCTGGCCCTGGGCAGCCCCGAGGCCCACGGCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCgcag                                                                                                                                     >  3:67680/1‑148 (MQ=255)
                      ccTGGCCGACTATCACCTGTTCCGCGGCCTGGCCCTGGGCAGCCCCGAGGCCCACGGCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGGTTGAAGCCGGcccagc                                                                                                                                            <  3:338555/137‑5 (MQ=255)
                      ccTGGCCGACTATCACCTGTTCCGCGGCCTGGCCCTGGGCAGCCCCGAGGCCCACGGCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGGTTGAAGCCGGcccagc                                                                                                                                            >  4:338555/1‑133 (MQ=255)
                         ggCCGACTATCACCTGTTCCGCGGCCTGGCCCTGGGCAGCCCCGAGGCCCACGGCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGt                                                                                                                             >  5:198939/1‑149 (MQ=255)
                          gCCGACTATCACCTGTTCCGCGGCCTGGCCCTGGGCAGCCCCGAGGCCCACGGCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGt                                                                                                                             <  2:53123/148‑1 (MQ=255)
                            cGACTATCACCTGTTCCGCGGCCTGGCCCTGGGCAGCCCCGAGGCCCACGGCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCtt                                                                                                                                           <  3:333078/132‑1 (MQ=255)
                            cGACTATCACCTGTTCCGCGGCCTGGCCCTGGGCAGCCCCGAGGCCCACGGCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCtt                                                                                                                                           >  4:333078/1‑132 (MQ=255)
                                 aTCACCTGTTCCGCGGCCTGGCCCTGGGCAGCCCCGAGGCCCACGGCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATc                                                                                                                     >  3:56684/1‑149 (MQ=255)
                                  tCACCTGTTCCGCGGCCTGGCCCTGGGCAGCCCCGAGGCCCACGGCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATc                                                                                                                     >  1:224651/1‑148 (MQ=255)
                                    aCCTGTTCCGCGGCCTGGCCCTGGGCAGCCCCGAGGCCCACGGCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGa                                                                                                                   <  4:67680/148‑1 (MQ=255)
                                     ccTGTTCCGCGGCCTGGCCCTGGGCAGCCCCGAGGCCCACGGCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAgg                                                                                                                 <  2:224651/149‑1 (MQ=255)
                                      cTGTTCCGCGGCCTGGCCCTGGGCAGCCCCGAGGCCCACGGCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAggg                                                                                                                >  4:299696/1‑149 (MQ=255)
                                         ttCCGCGGCCTGGCCCTGGGCAGCCCCGAGGCCCACGGCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGt                                                                                                             <  2:354216/149‑1 (MQ=255)
                                             gcgGCCTGGCCCTGGGCAGCCCCGAGGCCCACGGCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATc                                                                                                         <  4:89074/149‑1 (MQ=255)
                                                 ccTGGCCCTGGGCAGCCCCGAGGCCCACGGCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGcc                                                                                                      >  5:36845/1‑148 (MQ=255)
                                                   tGGCCCTGGGCAGCCCCGAGGCCCACGGCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAg                                                                                                   >  5:379920/1‑149 (MQ=255)
                                                     gCCCTGGGCAGCCCCGAGGCCCACGGCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAg                                                                                                   <  8:292434/147‑1 (MQ=255)
                                                           ggCAGCCCCGAGGCCCACGTCAGCCTTGCCGACAAGCTGAGCGAGCTGCAGGCCCTGCGCTAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAg                                                                                            <  3:288656/148‑1 (MQ=255)
                                                           ggCAGCCCCGAGGCCCACGGCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAg                                                                                            <  3:293694/148‑1 (MQ=255)
                                                               gCCCCGAGGCCCACGGCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGAc                                                                                       >  6:29133/1‑149 (MQ=255)
                                                                ccccGAGGCCCACGGCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACg                                                                                      <  4:186246/149‑1 (MQ=255)
                                                                  ccGAGGCCCACGGCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGc                                                                                    >  1:243106/1‑149 (MQ=255)
                                                                  ccGAGGCCCACGGCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGACCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACACGGCCACCTTCCGCACCAACCACTTGCTAATCGAGGGCGTGATCGCCAACCTCGAAGGCGACGGc                                                                                    >  1:243104/1‑149 (MQ=255)
                                                                       gCCCACGGCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGc                                                                               <  5:242709/149‑1 (MQ=255)
                                                                         ccACGGCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCcg                                                                             <  8:30757/149‑1 (MQ=255)
                                                                         ccACGGCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCcg                                                                             >  2:143099/1‑149 (MQ=255)
                                                                         ccACGGCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCcg                                                                             >  7:253341/1‑149 (MQ=255)
                                                                          cACGGCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGa                                                                                              <  8:282182/131‑1 (MQ=255)
                                                                          cACGGCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGa                                                                                              >  7:282182/1‑131 (MQ=255)
                                                                           aCGGCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCcgc                                                                            >  3:153047/1‑148 (MQ=255)
                                                                              gCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATc                                                                        >  3:166281/1‑149 (MQ=255)
                                                                               cAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATcc                                                                       <  3:93291/149‑1 (MQ=255)
                                                                               cAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATcc                                                                       <  7:212738/149‑1 (MQ=255)
                                                                               cAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATcc                                                                       <  2:12014/149‑1 (MQ=255)
                                                                                  ccTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCgct                                                                    <  8:127952/149‑1 (MQ=255)
                                                                                  ccTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCgct                                                                    <  6:36845/149‑1 (MQ=255)
                                                                                         gACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGa                                                             <  4:166281/149‑1 (MQ=255)
                                                                                              gCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGATGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAgg                                                        >  8:329200/1‑149 (MQ=255)
                                                                                              gCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAgg                                                        >  3:220320/1‑149 (MQ=255)
                                                                                               cTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAGgc                                                       <  4:56684/149‑1 (MQ=255)
                                                                                                tGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAGgcg                                                      >  3:373155/1‑149 (MQ=255)
                                                                                                    cgAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGG‑GTTATTGCCAAGCTAGAAGGCGAAGGCCATGCCGCGATACGCTTCTTAGTACAGGCCCaaa                                                  >  8:11511/1‑148 (MQ=255)
                                                                                                          gCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAGGCGCAAATcgccg                                            >  4:129063/1‑149 (MQ=255)
                                                                                                              gCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAGGCGCAAATCGCCGCCAc                                        >  2:32804/1‑149 (MQ=255)
                                                                                                               cccTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAGGCGCAAATCGCCGCCAcc                                       <  8:253341/149‑1 (MQ=255)
                                                                                                                ccTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCCAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAGGCGCAAATCGCCGCCACCg                                      >  2:18610/1‑149 (MQ=255)
                                                                                                                ccTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAGGCGCAAATCGCCGCCACCg                                      >  1:322113/1‑149 (MQ=255)
                                                                                                                    cgcgAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAGGCGCAAATCGCCGCCACCGCTGc                                  <  1:125269/149‑1 (MQ=255)
                                                                                                                    cgcgAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAGGCGCAAATCGCCGCCACCGCTGc                                  <  5:242428/149‑1 (MQ=255)
                                                                                                                       gagcgaTTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAGGCGCAAATcgcc                                             >  3:68430/1‑135 (MQ=255)
                                                                                                                       gagcgaTTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAGGCGCAAATcgcc                                             <  4:68430/135‑1 (MQ=255)
                                                                                                                       gagcgaTTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAGGCGCAAATCGCCGCCACCGCTGCCgg                               >  1:248752/1‑149 (MQ=255)
                                                                                                                       gagcgaTTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAGGCGCAAATCGCCGCCACCGCTGCCgg                               >  4:7484/1‑149 (MQ=255)
                                                                                                                        agcgaTTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAGGCGCAAATCGCCGCCACCGCTGCCGGc                              <  4:153047/149‑1 (MQ=255)
                                                                                                                         gcgaTTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAGGCGCAAATCGCCGCCACCGCTGCCGGCt                             >  3:215786/1‑149 (MQ=255)
                                                                                                                          cgaTTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAGGCGCAAATCGCCGCCACCGCTGCCGGCtt                            <  6:198939/149‑1 (MQ=255)
                                                                                                                           gaTTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAGGCGCAAATCGCCGCCACCGCTGCCGGCtt                            >  6:75251/1‑148 (MQ=255)
                                                                                                                           gaTTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAGGCGCAAATCGCCGCCACCGCTGCCGGCTTc                           <  3:196505/149‑1 (MQ=255)
                                                                                                                              tCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGAcc                                                           <  3:16404/114‑1 (MQ=255)
                                                                                                                              tCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGAcc                                                           >  4:16404/1‑114 (MQ=255)
                                                                                                                                 gCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAGGCGCAAATCGCCGCCACCGCTGCCGGCTTCATCCAc                     <  7:329200/149‑1 (MQ=255)
                                                                                                                                 gCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAGGCGCAAATCGCCGCCACCGCTGCCGGCTTCATCCAc                     >  6:74992/1‑149 (MQ=255)
                                                                                                                                  ccGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAGGCGCAAATCGCCGCCACCGCTGCCGGc                              <  3:357068/139‑1 (MQ=255)
                                                                                                                                  ccGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAGGCGCAAATCGCCGCCACCGCTGCCGGc                              >  4:357068/1‑139 (MQ=255)
                                                                                                                                   cGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAGGCGCAAATCGCCGCCACCGCTGCCGGCTTCATCCACGa                   <  2:322113/149‑1 (MQ=255)
                                                                                                                                   cGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAGGCGCAAATCGCCGCCACCGCTGCCGGCTTCATCCACGa                   <  3:299696/149‑1 (MQ=255)
                                                                                                                                   aaggtggCCGGGTTGAAGCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAGGCGCAAATCGCCGCCACCGCTGCCGGCTTCATCCAc                     >  5:306978/6‑147 (MQ=255)
                                                                                                                                    gCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAGGCGCAAATCGCCGCCACCGCTGCCGGCTTCATCCACGAg                  <  3:176142/149‑1 (MQ=255)
                                                                                                                                    gCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAGGCGCAAATCGCCGCCACCGCTGCCGGCTTCATCCACGAg                  >  7:312828/1‑149 (MQ=255)
                                                                                                                                    gCTGGGCCAGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTACTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAGGCGCAAATCGCCGCCACCGCTGCCGGCTTCATCCACGAg                  >  8:55955/1‑149 (MQ=255)
                                                                                                                                      tGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAGGCGCAAATCGCCGCCACCGCTGCCGGCTTCATCCACGAg                  >  3:141127/1‑147 (MQ=255)
                                                                                                                                      gtggCCGGGTTGAAGCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCCAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAGGCGCAAATCGCCGCCACCGCTGCCGGCTTCATCCACGAg                  >  5:188131/3‑147 (MQ=255)
                                                                                                                                       gggCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAGGCGCAAATCGCCGCCACCGCTGCCGGCTTCATCCACGAGCAg               >  3:245237/1‑149 (MQ=255)
                                                                                                                                        ggCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAGGCGCAAATCGCCGCCACCGCTGCCGGCTTCATCCACGAGCa                <  6:379920/147‑1 (MQ=255)
                                                                                                                                          ccGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAGGCGCAAATCGCCGCCACCGCTGCCGGCTTCATCCACGAGCAGgcg            >  7:27786/1‑149 (MQ=255)
                                                                                                                                          ccGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAGGCGCAAATCGCCGCCACCGCTGCCGGCTTCATCCACGAGCAGgcg            >  3:216457/1‑149 (MQ=255)
                                                                                                                                             gCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAGGCGCAAATCGCCGCCACCGCTGCCGGCTTCATCCACGAGCAGGCGCTg         >  7:258745/1‑149 (MQ=255)
                                                                                                                                              cTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAGGCGCAAATCGCCGCCACCGCTGCCGGCTTCATCCACGAGCAGGCGCTgg        <  4:373155/149‑1 (MQ=255)
                                                                                                                                               ttCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGACTGGCCGCGATCCGCTGCTTCGACCAGGCGCAAATCGCCGCCACCGCTGCCGGCTTCATCCACGAGCAGGCGCTGGc       >  6:269474/1‑149 (MQ=255)
                                                                                                                                                 cAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAGGCGCAAATCGCCGCCACCGCTGCCGGCTTCATCCACGAGCAGGCGCTGGc       <  4:215786/147‑1 (MQ=255)
                                                                                                                                                  aaCCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAGGCGCAAATCGCCGCCACCGCTGCCGGCTTCATCCACGAGCAGGCGCTGGccc     <  4:27402/148‑1 (MQ=255)
                                                                                                                                                  aaCCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAGGCGCAAATCGCCGCCACCGCTGCCGGCTTCATCCACGAGCAGGCGCTGGCCCa    >  8:262675/1‑149 (MQ=255)
                                                                                                                                                  aaCCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAGGCGCAAATCGCCGCCACCGCTGCCGGCTTCATCCACGAGCAGGCGCTGGCCCa    <  2:318325/149‑1 (MQ=255)
                                                                                                                                                  aaCCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAGGCGCAAATCGCCGCCACCGCTGCCGGCTTCATCCACGAGCAGGCGCTGGCCCa    <  1:143099/149‑1 (MQ=255)
                                                                                                                                                    cccGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAGGCGCAAATCGCCGCCACCGCTGCCGGCTTCATCCACGAGCAGGCGCTGGCCCATg  <  8:258745/149‑1 (MQ=255)
                                                                                                                                                    cccGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAGGCGAAAATCGCCGCCACCGCTGCCGGCTTCATCCACGAGCAGGCGCTGGCCCAt   >  7:331976/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GGGCCGCACCGGCGCACATCAACCTGGCCGACTATCACCTGTTCCGCGGCCTGGCCCTGGGCAGCCCCGAGGCCCACGGCAGCCTTGCCGACAAGCTGCGCGAGCTGCAGGCCCTGCGCGAGCGATTCAGCCGCTGGGCCGGCTTCAACCCGGCCACCTTCCGCAGCAAGCAGTTGCTAATCGAGGGCGTGATCGCCAAGCTCGAAGGCGACGGCCTGGCCGCGATCCGCTGCTTCGACCAGGCGCAAATCGCCGCCACCGCTGCCGGCTTCATCCACGAGCAGGCGCTGGCCCATG  >  NC_002947/5982660‑5982956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑