Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 550,549 C→G 6.0% intergenic (+30/‑112) tufB → / → rpsJ elongation factor Tu‑B/30S ribosomal protein S10

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947550,5490CG 6.0% 284.0 / 5.4 94intergenic (+30/‑112)tufB/rpsJelongation factor Tu‑B/30S ribosomal protein S10
Reads supporting (aligned to +/- strand):  ref base C (43/45);  new base G (2/4);  total (45/49)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

GATGACTGTTACCCTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAG  >  NC_002947/550408‑550693
                                                                                                                                             |                                                                                                                                                    
gATGACTGTTACCCTGATCAAGACCATCGCAATGGAAGTCTGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTTAAAAAACCCCCGCTCAGCggg‑g                                                                                                                                              <  3:46700/149‑1 (MQ=255)
gATGACTGTTACCCTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCggg‑g                                                                                                                                              <  7:162778/149‑1 (MQ=255)
gATGACTGTTACCCTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCggg‑g                                                                                                                                              >  2:180093/1‑149 (MQ=255)
  tGACTGTTACCCTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCgg                                                                                                                                                 <  1:147241/145‑1 (MQ=255)
  tGACTGTTACCCTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCgg                                                                                                                                                 >  2:147241/1‑145 (MQ=255)
   gACTGTTACCCTGATCAAGACAATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCTATTGAAAAAACCCCCGCACAGCGGG‑GG‑tt                                                                                                                                          >  2:186599/1‑149 (MQ=255)
    aCTGTTACCCTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTGAGCGGG‑GG‑tt                                                                                                                                          <  5:60681/148‑1 (MQ=255)
    aCTGTTACCCTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGGACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑ttt                                                                                                                                         <  7:242859/149‑1 (MQ=255)
      tGTTACCCTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑Gg‑ttgt‑t                                                                                                                                      >  6:100762/1‑149 (MQ=255)
       gTTACCCTGCTCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGGGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTGAGCGGG‑GG‑tttt‑tt                                                                                                                                     <  3:236885/149‑1 (MQ=255)
        ttACCCTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑tttt‑ttt                                                                                                                                    <  1:147189/149‑1 (MQ=255)
        ttACCCTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑tttt‑ttt                                                                                                                                    <  5:370638/149‑1 (MQ=255)
        ttACCCTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑tttt‑t                                                                                                                                      <  4:361999/147‑1 (MQ=255)
        ttACCCTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑tttt                                                                                                                                        <  7:14856/146‑1 (MQ=255)
        ttACCCTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑tttt                                                                                                                                        >  8:14856/1‑146 (MQ=255)
          aCCCTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑tttt‑tttt                                                                                                                                   <  2:228331/148‑1 (MQ=255)
          aCCCTGATCAAGACCATCGCAAAGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGAAAACACACGACCGATTGAAAAAACCCCCCCTCAGCGGG‑GC‑TTTT‑TTTTa                                                                                                                                  >  6:358688/1‑149 (MQ=21)
           cccTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTa                                                                                                                                  <  8:191625/148‑1 (MQ=255)
           cccTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTAt                                                                                                                                 <  4:192632/149‑1 (MQ=255)
            ccTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTGAGCGGG‑GG‑t                                                                                                                                           <  6:127231/139‑1 (MQ=255)
            ccTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTGAGCGGG‑GG‑t                                                                                                                                           >  5:127231/1‑139 (MQ=255)
            ccTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTAtt                                                                                                                                >  3:338743/1‑149 (MQ=255)
            ccTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTAtt                                                                                                                                >  8:277882/1‑149 (MQ=255)
                aTCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTggg                                                                                                                             <  7:277882/148‑1 (MQ=255)
                aTCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTggg                                                                                                                             <  8:303630/148‑1 (MQ=255)
                      aCCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGacac                                                                                                                      <  8:332831/149‑1 (MQ=255)
                       ccATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGacac                                                                                                                      >  2:260963/1‑148 (MQ=255)
                        cATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACt                                                                                                                     >  3:189877/1‑148 (MQ=255)
                        cATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑a                                                                                                                   >  8:121965/1‑149 (MQ=255)
                        cATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACAGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACt                                                                                                                     >  6:221434/1‑148 (MQ=255)
                         aTCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑‑TTT‑TTTTATTGGGTTGACACT‑AAt                                                                                                                 <  6:60194/149‑1 (MQ=255)
                         aTCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑‑TTT‑TTTTATTGGGTTGACACT‑AAt                                                                                                                 >  5:60194/1‑149 (MQ=255)
                          tCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AAt                                                                                                                 >  3:126390/1‑149 (MQ=255)
                             cAATGGAAGACGGTCTGCGCTTCGCTATCCGAGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAAACATCGAGTAAACACTCGACCGATTGAAAAAACCCCCGCTCAGCGGC‑GG‑TTTA‑TTATATTCGGTTGACACT‑AATTgg                                                                                                              >  3:75483/1‑149 (MQ=25)
                              aaTTGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGTCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTggg                                                                                                             <  5:200322/149‑1 (MQ=255)
                              aaTGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTggg                                                                                                             <  1:371709/149‑1 (MQ=255)
                               aTGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTgggg                                                                                                            <  3:105238/149‑1 (MQ=255)
                                  gAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGc                                                                                                          <  7:207839/148‑1 (MQ=255)
                                     gACGCTCTGCGCTTCGCTATCCGTGAACTCGGTCGTACCGTCGGCGCCGGCGTTGTGTCCAAAATCATCGAGTAATCCCTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCt                                                                                                      <  2:263136/149‑1 (MQ=38)
                                         gTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TATT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCta                                                                                                     >  6:226142/1‑146 (MQ=255)
                                          tCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCGCTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TGTT‑TTTGATTGGGTTGACACT‑AATTGGGGGCGTCTATAg                                                                                                  >  6:146469/1‑148 (MQ=255)
                                          tCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAg                                                                                                  >  5:116314/1‑148 (MQ=255)
                                          tCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAg                                                                                                  >  5:193820/1‑148 (MQ=255)
                                              cgcTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAGAATCa                                                                                             <  1:180093/149‑1 (MQ=255)
                                              cgcTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAGAATCa                                                                                             >  3:239982/1‑149 (MQ=255)
                                              cgcTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAGAATCa                                                                                             <  6:193820/149‑1 (MQ=255)
                                               gcTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAGAATCAc                                                                                            <  1:148760/149‑1 (MQ=255)
                                                 ttCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GGTTTTT‑TTTTTTTGGGTTGCCACT‑AATTGGGGGCGTCTATAGAATCaag                                                                                           >  8:165093/1‑147 (MQ=255)
                                                 ttCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAGAATCACGc                                                                                          >  7:59626/1‑149 (MQ=255)
                                                     cTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAGAATCACGcctcc                                                                                      <  5:100762/149‑1 (MQ=255)
                                                     cTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAGAATCACGcctcc                                                                                      >  4:9625/1‑149 (MQ=255)
                                                      tATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAGAATCACGcctcct                                                                                     >  1:229548/1‑149 (MQ=255)
                                                           gTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGAGTTGACACTAAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTa                                                                                 <  1:50650/149‑1 (MQ=255)
                                                             gAAGGCGGTCGGACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGGTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCg                                                                              <  5:358688/149‑1 (MQ=255)
                                                                 gCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCg                                                                          >  3:181397/1‑149 (MQ=255)
                                                                   ggTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGATTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTa                                                                        <  4:189877/149‑1 (MQ=255)
                                                                   ggTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTa                                                                        <  5:48843/149‑1 (MQ=255)
                                                                       gTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GGTTTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGt                                                                      <  7:165093/148‑1 (MQ=255)
                                                                         aCCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTTTTTGGTTGAAACT‑AATTTGGGGCGTCTATTGAATAAAGGCTACGTTTTGGGGGGGTAGTGCGt                                                                  >  5:35234/1‑149 (MQ=255)
                                                                             tCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGt                                                              >  6:163832/1‑149 (MQ=255)
                                                                             tCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGt                                                              >  1:2823/1‑149 (MQ=255)
                                                                               ggCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTg                                                            <  7:121965/149‑1 (MQ=255)
                                                                                gcgcCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTgg                                                           <  5:112156/149‑1 (MQ=255)
                                                                                    cGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAAc                                                       <  5:163832/149‑1 (MQ=255)
                                                                                     ggCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGaa                                                        >  3:278323/1‑147 (MQ=255)
                                                                                        gTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGcc                                                   >  5:27581/1‑149 (MQ=255)
                                                                                           gtgGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTg                                                 <  1:186599/148‑1 (MQ=255)
                                                                                            tgGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGa                                               <  4:239982/149‑1 (MQ=255)
                                                                                              gCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAACCCCCCGCTCAGCGGGAAG‑TTTT‑TTTTATTGGGTTGACAAT‑AATTGGTGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGGGCGTCCGTTGGGAACCGCCTGGAg                                              >  1:103840/1‑149 (MQ=255)
                                                                                                 caaaTCATCGAGTAATCACTCGACCGATTGAAAAAACCCCAGCTCAGCGGG‑GG‑TTTTATTTTATTGGGTTTACACT‑AATTTGGGTCGTATAAAGAATCACGAATCATTTTAGCGGGCGTATTGCGTCCGTTGGGAACAGCATGGAGTCt                                           >  5:354697/2‑149 (MQ=255)
                                                                                                 aaaaTCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTg                                          <  6:27581/149‑1 (MQ=255)
                                                                                                    atcatcGAGTAATCACTCGTCCGTTTGAAAATACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑ATTTGGGGGCGTCTATAGATTGACGCCTCGTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGTGTCTGtaa                                       <  4:75483/149‑1 (MQ=255)
                                                                                                    atcatcGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGaaa                                       >  3:186036/1‑149 (MQ=255)
                                                                                                    atcatcGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGaaa                                       <  7:16855/149‑1 (MQ=255)
                                                                                                       atcGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATc                                     <  4:126390/148‑1 (MQ=255)
                                                                                                          gAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAAt                                 >  6:334878/1‑149 (MQ=255)
                                                                                                           aGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGCGTCTGAAATCCAATg                                >  2:293088/1‑149 (MQ=255)
                                                                                                           aGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGAAACT‑AATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATg                                >  2:297803/1‑149 (MQ=255)
                                                                                                             tAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCaa                                  <  8:296010/145‑1 (MQ=255)
                                                                                                             tAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCaa                                  >  7:296010/1‑145 (MQ=255)
                                                                                                              aaTCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCa                              <  4:186036/148‑1 (MQ=255)
                                                                                                               aTCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCaa                             >  4:36068/1‑148 (MQ=255)
                                                                                                                      gACCGATAGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTCCCCCT‑AATTGGGGGCGTCTATAGAATCAAGCCTCCTTTTAGAGGGCGTAGTTCGTCCGTTGGGAACAGCCTGGAATCAGAAATCCAATGCACAATCAGc                      >  6:163351/1‑148 (MQ=255)
                                                                                                                       aCCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCa                     >  5:102168/1‑148 (MQ=255)
                                                                                                                          gATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATc                 >  3:39/1‑149 (MQ=255)
                                                                                                                               aaaaaaCCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTa             >  6:122836/1‑148 (MQ=255)
                                                                                                                                acaaaCCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTAt            <  3:9625/146‑1 (MQ=255)
                                                                                                                                aaaaaCCCCCGCTGAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTAt            >  1:107630/1‑148 (MQ=255)
                                                                                                                                   aaCCCCCGCTGAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAgg        <  2:107630/149‑1 (MQ=255)
                                                                                                                                       cccGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCaa                                  <  6:234235/119‑1 (MQ=255)
                                                                                                                                       cccGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCaa                                  >  5:234235/1‑119 (MQ=255)
                                                                                                                                        ccGCTCAGCGGG‑TG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGaa   <  5:221434/149‑1 (MQ=255)
                                                                                                                                        ccGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGaa   <  8:59626/149‑1 (MQ=255)
                                                                                                                                         cGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAg  <  5:226142/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                    
GATGACTGTTACCCTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGG‑GG‑TTTT‑TTTTATTGGGTTGACACT‑AATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAG  >  NC_002947/550408‑550693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑