Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,073,448 G→A 8.3% Q270* (CAG→TAG) ‡ PP_5327 ← phosphate ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,073,4480GA 8.3% 277.8 / 10.2 96Q270* (CAG→TAG) ‡PP_5327phosphate ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (48/40);  new base A (6/2);  total (54/42)
Fisher's exact test for biased strand distribution p-value = 4.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCAGGGTCATCATCACGGTACCGAAGATGGCCGGGAAGATACCGCCCTCGGTGTTGGCTTCACGCGGGTCGTCGCTGAGGAACTCCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCTGGTGCAGGCCGGTCAGGCGGTCTTCGATAGCCTTGTAGCGGCTGTTCAGTTCGGCACGGTC  >  NC_002947/6073307‑6073595
                                                                                                                                             |                                                                                                                                                   
tCAGGGTCATCATCACGGTACCGAAGATGGCCGGGAAGATACCGCCCTCGGTGTTGGCTTCACGCGGGTCGTCGCTGAGGAACTCCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCa                                                                                                                                              >  4:256421/1‑149 (MQ=255)
           atcaCGGTACCGAAGATGGCCGGGAAGATACCGCCCTCGGTGTTGGCTTCACGCGGGTCGTCGCTGAGGAACTCCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCtt                                                                                                                                   >  8:294306/1‑149 (MQ=255)
            tcaCGGTACCGAAGATGGCCGGGAAGATACCGCCCTCGGTGTTGGCTTCACGCGGGTCGTCGCTGAGGAACTCCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTg                                                                                                                                  <  4:176710/149‑1 (MQ=255)
             caCGGTACCGAAGATGGCCGGGAAGATACCGCCCTCGGTGTTGGCTTCACGCGGGTAGTCCCTGAGGAACTCCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGc                                                                                                                                 >  2:57613/1‑149 (MQ=255)
                  tACCGAAGATGGCCGGGAAGATACCGCCCTCGGTGTTGGCTTCACGCGGGTCGTCGCTGAGGAACTCCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAgg                                                                                                                            <  6:360765/149‑1 (MQ=255)
                    ccGAAGATGGCCGGGAAGATACCGCCCTCGGTGTTGGCTTCACGCGGGTCGTCGATGAGGAACTCCCAGACATTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGATGAACGGCATGCACTACCTTGCTCAGGtt                                                                                                                          >  5:318483/1‑149 (MQ=255)
                      gAAGATGGCCGGGAAGATACCGCCCTCGGTGTTGGCTTCACGCGGGTCGTCGCTGAGGAACTCCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGa                                                                                                                        >  7:90601/1‑149 (MQ=255)
                         gATGGCCGGGAAGATACCGCCCTCGGTGTTGGCTTCACGCGGGTCGTCGCTGAGGAACTCCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGAttt                                                                                                                     >  3:89349/1‑149 (MQ=255)
                            ggCCGGGAAGATACCGCCCTCGGTGTTGGCTTCACGCGGGTCGTCGCTGAGGAACTCCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCa                                                                                                                  >  3:38334/1‑149 (MQ=255)
                             gCCGGGAAGATACCGCCCTCGGTGTTGGCTTCACGCGGGTCGTCGCTGAGGAACTCCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCa                                                                                                                  <  7:99427/148‑1 (MQ=255)
                             gCCGGGAAGATACCGCCCTCGGTGTTGGCTTCACGCGGGTCGTCGCTGAGGAACTCCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCa                                                                                                                  <  2:64594/148‑1 (MQ=255)
                              ccGGGAAGATACCGCCCTCGGTGTTGGCTTCACGCGGGTCGTCGCTGAGGAACTCCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACt                                                                                                                >  1:205681/1‑149 (MQ=255)
                               cGGGAAGATACCGCCCTCGGTGTTGGCTTCACGCGGGTCGTCGCTGAGGAACTCCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACtt                                                                                                               >  6:334747/1‑149 (MQ=255)
                                  gAAGATACCGCCCTCGGTGTTGGCTTCACGCGGGTCGTCGCTGAGGAACTCCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCAc                                                                                                            <  3:256421/149‑1 (MQ=255)
                                      aTACCGCCCTCGGTGTTGGCTTCACGCGGGTCGTCGCTGAGGAACTCCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGcc                                                                                                        >  8:278189/1‑149 (MQ=255)
                                       tACCGCCCTCGGTTGTGGCTTCACGCGGGTCCTCGCTGAGGAACTGCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCg                                                                                                       <  8:34012/149‑1 (MQ=255)
                                        aCCGCCCTCGGTGTTGGCTTCACGCGGGTCGTCGCTGAGGAACTCCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGt                                                                                                      >  2:95223/1‑149 (MQ=255)
                                          cGCCCTCGGTGTTGGCTTCACGCGGGTCGTCGCTGAGGAACTCCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTg                                                                                                    >  5:165536/1‑149 (MQ=255)
                                          cGCCCTCGGTGTTGGCTTCACGCGGGTCGTCGCTGAGGAACTCCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTg                                                                                                    >  6:172239/1‑149 (MQ=255)
                                           gCCCTCGGTGTTGGCTTCACGCGGGTCGTCGCTGAGGAACTCCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGc                                                                                                   <  4:155579/149‑1 (MQ=255)
                                                   tgtTGGCTTCACGCGGGTCGTCGCTGAGGAACTCCCAGACCTTGCCGAAGTAGGTGCCCAAATTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCAGTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCgg                                                                                           >  1:106904/1‑149 (MQ=255)
                                                     ttGGCTTCACGCGGGTCGTCGCTGAGGAACTCCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCggg                                                                                          <  1:95223/148‑1 (MQ=255)
                                                        gCTTCACGCGGGTCGTCGCTGAGGAACTCCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGccac                                                                                      >  3:238089/1‑149 (MQ=255)
                                                          ttCACGCGGGTCGTCGCTGAGGAACTCCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGccac                                                                                      >  1:180986/1‑147 (MQ=255)
                                                          ttCACGCGGGTCGTCGCTGAGGAACTCCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGccac                                                                                      >  6:32203/1‑147 (MQ=255)
                                                             aCGCGGGTCGTCGCTGAGGAACTCCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGc                                                                                 >  7:224448/1‑149 (MQ=255)
                                                             aCGCGGGTCGTCGCTGAGGAACTCCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGc                                                                                 >  3:136717/1‑149 (MQ=255)
                                                             aCGCGGGTCGTCGCTGAGGAACTCCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGc                                                                                 >  7:353432/1‑149 (MQ=255)
                                                             aCGCGGGTCGTCGCTGAGGAACTCCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGc                                                                                 >  6:213723/1‑149 (MQ=255)
                                                                   gtcgtcGCTGAGGAACTCCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTcgc                                                                           >  7:214310/1‑149 (MQ=255)
                                                                      gtcgCTGAGGAACTCCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCgg                                                                         <  4:38334/148‑1 (MQ=255)
                                                                      gtcgCTGAGGAACTCCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCgg                                                                         <  1:57613/148‑1 (MQ=255)
                                                                         gCTGAGGAACTCCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGaa                                                                      <  2:180986/148‑1 (MQ=255)
                                                                            gAGGAACTCCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCgg                                                                                           >  5:321031/1‑124 (MQ=255)
                                                                            gAGGAACTCCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCgg                                                                                           <  6:321031/124‑1 (MQ=255)
                                                                             aGGAACTCCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGaaaaa                                                                   <  6:165536/147‑1 (MQ=255)
                                                                             aGGAACTCCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGGGAACGCCGACCTGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCTGGaaaaacc                                                                 <  5:116443/149‑3 (MQ=255)
                                                                               gAACTCCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCt                                                               >  4:171671/1‑149 (MQ=255)
                                                                               gAACTCCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCt                                                               >  3:115320/1‑149 (MQ=255)
                                                                                 aCTCCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCTgg                                                             <  5:172239/149‑1 (MQ=255)
                                                                                   tCCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCTGGt                                                            >  3:226437/1‑148 (MQ=255)
                                                                                   tCCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCTGGTg                                                           >  5:280587/1‑149 (MQ=255)
                                                                                   tCCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCTGGTg                                                           >  2:328418/1‑149 (MQ=255)
                                                                                     ccAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCgg                                                                                           <  5:182967/115‑1 (MQ=255)
                                                                                     ccAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCgg                                                                                           >  6:182967/1‑115 (MQ=255)
                                                                                     ccAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGccac                                                                                      >  4:255054/1‑120 (MQ=255)
                                                                                     ccAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGccac                                                                                      <  3:255054/120‑1 (MQ=255)
                                                                                     ccAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGaaaaa                                                                   >  7:166237/1‑139 (MQ=255)
                                                                                     ccAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGaaaaa                                                                   <  8:166237/139‑1 (MQ=255)
                                                                                     ccAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCTGGt                                                            <  4:226437/146‑1 (MQ=255)
                                                                                     ccAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCTGGTg                                                           >  2:91844/1‑147 (MQ=255)
                                                                                      cAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCTGGTGCAg                                                        <  5:32203/149‑1 (MQ=255)
                                                                                      cAGACCTTGCCGAAGTAGGTG‑CCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCTGGTGCAgg                                                       >  6:281053/1‑149 (MQ=255)
                                                                                       aGACCTTGCCGAAGTAGGTGCCCAACTCGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCTGGTGCAgg                                                       >  3:352538/1‑149 (MQ=255)
                                                                                            ttGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCTGGTGCAGGCCGGt                                                  >  2:101044/1‑149 (MQ=255)
                                                                                               ccGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCgg                                                                                           >  7:291749/1‑105 (MQ=255)
                                                                                               ccGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTGTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCgg                                                                                           <  8:291749/105‑1 (MQ=255)
                                                                                               acGAAGTAGGTGACCAAATTGGTGAACGCCGACATGCCGTTTTGCTGAACGGCATGCACTACCTTTCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCTGGTGCAGGCCGGTCa                                                <  6:318483/147‑1 (MQ=255)
                                                                                                cGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCTGGTGCAGGCCGGTCAgg                                              >  2:63493/1‑149 (MQ=255)
                                                                                                 gAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCTGGTGCAGGCCGGTCAGGc                                             <  5:334747/149‑1 (MQ=255)
                                                                                                  aaGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCTGGTGCAGGCCGGTCAGGCg                                            >  2:292630/1‑149 (MQ=255)
                                                                                                          gCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCTGGTGCAGGCCGGTCAGGCGGTCTTCGa                                    >  2:189381/1‑149 (MQ=255)
                                                                                                           cccAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCTGGTGCAGGCCGGTCAGGCGGTCTTCGAt                                   <  7:294306/149‑1 (MQ=255)
                                                                                                            ccAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCTGGTGCAGGCCGGTCAGGCGGTCTTCGATa                                  <  1:91844/149‑1 (MQ=255)
                                                                                                            ccAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCTGGTGCAGGCCGGTCAGGCGGTCTTCGATa                                  <  8:353432/149‑1 (MQ=255)
                                                                                                              aaCTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCTGGTGCAGGCCGGTCAGGCGGTCTTCGATAGc                                <  8:214310/149‑1 (MQ=255)
                                                                                                               aCTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCTGGTGCAGGCCGGTCAGGCGGTCTTCGATAGcc                               <  8:224448/149‑1 (MQ=255)
                                                                                                               aCTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCTGGTGCAGGCCGGTCAGGCGGTCTTCGATAGcc                               <  4:136717/149‑1 (MQ=255)
                                                                                                                 ttGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCTGGTGCAGGCCGGTCAGGCGGTCTTCGATAGCCtt                             <  4:89349/149‑1 (MQ=255)
                                                                                                                   ggTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCTGGTGCAGGCCGGTCAGGCGGTCTTCGATAGCCtt                             <  1:189381/147‑1 (MQ=255)
                                                                                                                   ggTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCTGGTGCAGGCCGGTCAGGCGGTCTTCGATAGCCTTg                            <  4:238089/148‑1 (MQ=255)
                                                                                                                     tcaaCTCCGACATGACTTTTGGCTGAACGGCATGCACAAACTTCCTAAGGTTGATTTCCACTTCACGGACGTTGACATCCCCGGACACAAGGATGTATCGCGAAAAAGCCTGGTGCAGGCCGCTAAGGCGGTCTTCGATAGCCttc                            >  6:222348/3‑145 (MQ=255)
                                                                                                                       aaCGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGTTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCTGGTGCAGGCCGGTCAGGCGGTCTTCGATAGCCTTGTAGCg                       >  1:60068/1‑149 (MQ=255)
                                                                                                                        aCGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCTGGTGCAGGCCGGTCAGGCGGTCTTCGATAGCCTTGTAGCgg                      <  6:82848/149‑1 (MQ=255)
                                                                                                                         cGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCTGGTGCAGGCCGGTCAGGCGGTCTTCGATAGCCTTGTAGCGGc                     <  5:281053/149‑1 (MQ=255)
                                                                                                                          gCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCTGGTGCAGGCCGGTCAGGCGGTCTTCGATAGCCTTGTAGCGGCt                    >  6:41795/1‑149 (MQ=255)
                                                                                                                             tgcATGCCGTTCAGCCAAACGGCATGTACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCt                                                               <  6:262799/101‑1 (MQ=38)
                                                                                                                             tgcATGCCGTTCAGCCAAACGGCATGTACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCt                                                               >  5:262799/3‑103 (MQ=38)
                                                                                                                             tgcATGCCGTTCAGCCAAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCTGGTGCAGGCCGGTCAGGCGGTCTTCGATAGCCTTGTAGCGGCTGtt                 >  5:328767/3‑149 (MQ=255)
                                                                                                                             gACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCTGGTGCAGGCCGGTCAGGCGGTCTTCGATAGCCTTGTAGCGGCTGtt                 <  4:115320/149‑1 (MQ=255)
                                                                                                                               cATGCCGTTCAGCCAAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCTGGTGCAGGCCGGTCAGGCGGTCTTCGATAGCCTTGTAGCGGCTGtt                 >  6:179796/1‑147 (MQ=255)
                                                                                                                               cATGCCGTTCAGCCAAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCTGGTGCAGGCCGGTCAGGCGGTCTTCGATAGCCTTGTAGCGGCTGTTCa               >  3:21349/1‑149 (MQ=255)
                                                                                                                                aTGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAGAGCCTGGTGCAGGCCGGTCAGGc                                             >  8:97013/1‑118 (MQ=255)
                                                                                                                                aTGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAACAGCCTGGTGCAGGCCGGTCAGGc                                             <  7:97013/118‑1 (MQ=255)
                                                                                                                                aTGCCGTTCAGCCAAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCTGGTGCAGGCCGGTCAGGCGGTCTTCGATAGCCTTGTAGCGGCTGTTCAg              >  4:184491/1‑149 (MQ=255)
                                                                                                                                aTGCCGTTCAGCCAAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCTGGTGCAGGCCGGTCAGGCGGTCTTCGATAGCCTTGTAGCGGCTGTTCAg              <  3:184491/149‑1 (MQ=255)
                                                                                                                                 tGCCGTTCAGCCAAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCTGGTGCAGGCCGGTCAGGCGGTCTTCGATAGCCTTGTAGCGGCTGTTCAGt             >  2:252861/1‑149 (MQ=255)
                                                                                                                                  gCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCTGGTGCAGGCCGGTCAGGCGGTCTTCGATAGCCTTGTa                          <  4:158922/135‑1 (MQ=255)
                                                                                                                                  gCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCTGGTGCAGGCCGGTCAGGCGGTCTTCGATAGCCTTGTa                          >  3:158922/1‑135 (MQ=255)
                                                                                                                                  gCCGTTTGGCTGAACGGCATGCACTACCGTGCTCAGGGTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAACGCCTGGTGCAGGCCGGTCAGGCGGACTACGATAGCCTTGTAGCGGCTGtt                 >  5:282544/1‑144 (MQ=255)
                                                                                                                                  gCCGGTTGGCTGATCGGCATGCACTACCTTGCTCAGGTTGTTTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCTGGTGCAGGCCGGTCAGGCGGTCTTCGATAGCCTTGTAGCGGCTGtt                 <  6:282544/144‑1 (MQ=255)
                                                                                                                                   ccGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCTGGTGCAGGCCGGTCAGGCGGTCTTCGATAGCCTTGTAGCGGCTGTTCAGTTc           >  1:98909/1‑149 (MQ=255)
                                                                                                                                          gCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCTGGTGCAGGCCGGTCAGGCGGTCTTCGATAGCCTTGTAGCGGCTGTTCAGTTCGGCACg     <  6:280587/148‑1 (MQ=255)
                                                                                                                                           cTGAAGGGCATGAACTTCCTTGATCAGGTTTATTTCCAGTTTACGGCCGTAGCCATGCGGGTCCACCAGGCTGTAGCTGGAAAAAGACTGGTGCAGGCGGTTCATGAGGTCTTCTATAGCCTTGTAGCGGCTGTTCAGTTCGGCACgg    <  5:222348/148‑1 (MQ=255)
                                                                                                                                            tGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCTGGTGCAGGCCGGTCAGGCGGTCTTCGATAGCCTTGTAGCGGCTGTTCAGTTCGGCACGGTc  >  5:341150/1‑149 (MQ=255)
                                                                                                                                            tGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCTGGTGCAGGCCGGTCAGGCGGTCTTCGATAGCCTTGTAGCGGCTGTTCAGTTCGGCACGGTc  >  1:16960/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                   
TCAGGGTCATCATCACGGTACCGAAGATGGCCGGGAAGATACCGCCCTCGGTGTTGGCTTCACGCGGGTCGTCGCTGAGGAACTCCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACATGCCGTTTGGCTGAACGGCATGCACTACCTTGCTCAGGTTGATTTCCACTTCACGGCCGTTGCCATCCCGGGCCACCAGGCTGTCGCGGGAAAAAGCCTGGTGCAGGCCGGTCAGGCGGTCTTCGATAGCCTTGTAGCGGCTGTTCAGTTCGGCACGGTC  >  NC_002947/6073307‑6073595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑