Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,096,220 A→T 8.0% S160T (TCC→ACC)  pycA ← pyruvate carboxylase subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,096,2200AT 8.0% 203.6 / 4.5 71S160T (TCC→ACC) pycApyruvate carboxylase subunit A
Reads supporting (aligned to +/- strand):  ref base A (31/34);  new base T (3/3);  total (34/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGTGCTTAGGGTTGACGATGCATTTCTCCAGGAACACTTCGGCCGAACCGAATGCCTTGGTCGCCTCGGAAATGACCCGTGGGAAGTTCTGTTCCAGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTCAGGGCTTCATGAATATCGGCAACGTTACCTTCGGTGCCCGGGGTTACCGGCACACCGGCGGCGATCATGGTGCGGCGCGCTTCGGTCTTGTCGCCCATACGG  >  NC_002947/6096075‑6096368
                                                                                                                                                 |                                                                                                                                                    
aTGTGCTTAGGGTTGACGATGCATTTCTCCAGGAACACTTCGGCCGAACCGAATGCCTTGGTCGCCTCGGAAATGACCCGTGGGAAGTTCTGTTCCAGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGt                                                                                                                                                   <  2:280219/149‑1 (MQ=255)
 tgtgCTTAGGGTTGACGATGCATTTCTCCAGGAACACTTCGGCCGAACCGAATGCCTTGGTCGCCTCGGAAATGACCCGTGGGAAGTTCTGTTCCAGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTg                                                                                                                                                  <  5:116518/149‑1 (MQ=255)
    gCTTAGGGTTGACGATGCATTTCTCCAGGAACACTTCGGCCGAACCGAATGCCTTGGTCGCCTCGGAAATGACCCGTGGGAAGTTCTGTTCCAGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGcc                                                                                                                                               >  6:342964/1‑149 (MQ=255)
    gCTTAGGGTTGACGATGCATTTCTCCAGGAACACTTCGGCCGAACCGAATGCCTTGGTCGCCTCGGAAATGACCCGTGGGAAGTTCTGTTCCAGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGcc                                                                                                                                               >  4:297397/1‑149 (MQ=255)
        aGGGTTGACGATGCATTTCTCCAGGAACACTTCGGCCGAACCGAATGCCTTGGTCGCCTCGGAAATGCCCCGTGGGAAGTTCTGTTCCAGTTCTTCGCGGCTATTGCACCGGCGATTACCACGGCCGCCACCACCGGAGGTGGCCtt                                                                                                                                             >  2:309573/1‑147 (MQ=255)
        aGGGTTGACGATGCATTTCTCCAGGAACACTTCGGCCGAACCGAATGCCTTGGTCGCCTCGGAAATGACCCGTGGGAAGTTCTGTTCCAGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCtt                                                                                                                                             <  3:252386/147‑1 (MQ=255)
         gggTTGAGGATGCATTTCTCGAGGAAGACTTCGGCCGAACCGAATTCCTTGGTCTCCTCGGAAATGACGCGTTGGAAGTTCTGTTCGAGTTCTTCGCGGCTATTGCAACGGCGAATACGTGGGTCGCCAGGACCGTTGGTGGCGTTGAg                                                                                                                                          <  4:335100/149‑1 (MQ=255)
         gggTTGACGATGCATTTCTCCAGGAACACTTCGGCCGAACCGAATGCCTTGGTCGCCTCGGAAATGACCCGTGGGAAGTTCTGTTCCAGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGa                                                                                                                                           <  4:353786/148‑1 (MQ=255)
          ggTTGACGATGCATTTCTCCAGGAACACTTCGGCCGAACCGAATGCCTTGGTCGCCTCGGAAATGACCCGTGGGAAGTTCTGTTCCAGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAg                                                                                                                                          >  7:235533/1‑148 (MQ=255)
               aCGATGCATTTCTCCAGGAACACTTCGGCCGAACCGAATGCCTTGGTCTCCTCGGAAATGACCCGTGGGAAGTTCTGTTCCAGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCAc                                                                                                                                    <  1:104/149‑1 (MQ=255)
                cGATGCATTTCTCCAGGAACACTTCGGCCGAACCGAATGCCTTGGTCGCCTCGGAAATGACCCGTGGGAAGTTCTGTTCCAGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCAcc                                                                                                                                   <  8:235533/149‑1 (MQ=255)
                 gATGCATTTCTCCAGGAACACTTCGGCCGAACCGAATGCCTTGGTCGCCTCGGAAATGACCCGTGGGAAGTTCTGTTCCAGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCg                                                                                                                                  <  8:73477/149‑1 (MQ=255)
                   tGCATTTCTCCAGGAACACTTCGGCCGAACCGAATGCCTTGGTCGCCTCGGAAATGACCCGTGGGAAGTTCTGTTCCAGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGa                                                                                                                                <  8:98228/149‑1 (MQ=255)
                      aTTTCTCCAGGAACACTTCGGCCGAACCGAATGCCTTGGTCGCCTCGGAAATGACCCGTGGGAAGTTCTTTTCCAGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATaa                                                                                                                             >  4:275565/1‑149 (MQ=255)
                      aTTTCTCCAGGAACACTTCGGCCGAACCGAATGCCTTGGTCGCCTCGGAAATGACCCGTGGGAAGTTCTGTTCCAGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATaa                                                                                                                             >  8:76387/1‑149 (MQ=255)
                       tttCTCCAGGAACACTTCGGCCGAACCGAATGCCTTGGTCGCCTCGGAAATGACCCGTGGGAAGTTCTGTTCCAGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAAc                                                                                                                            <  2:111936/149‑1 (MQ=255)
                       tttCTCCAGGAACACTTCGGCCGAACCGAATGCCTTGGTCGCCTCGGAAATGACCCGTGGGAAGTTCTGTTCCAGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAAc                                                                                                                            >  6:7823/1‑149 (MQ=255)
                         tctcCAGGAACACTTCGGCCGAACCGAATGCCTTGGTCGCCTCGGAAATGACCCGTGGGAAGTTCTGTTCCAGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAAcc                                                                                                                           <  4:150140/148‑1 (MQ=255)
                               ggAACACTTCGGCCGAACCGAATGCCTTGGTCGCCTCGGAAATGACCCGTGGGAAGTTCTGTTCCAGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAACCGATGCgg                                                                                                                    <  4:178348/149‑1 (MQ=255)
                                gAACACTTCGGCCGAACCGAATGCCTTGGTCGCCTCGGAAATGACCCGTGGGAAGTTCTGTTCCAGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAACCGATGCGGt                                                                                                                   <  8:237129/149‑1 (MQ=255)
                                gAACACTTCGGCCGAACCGAATGCCTTGGTCGCCTCGGAAATGACCCGTGGGAAGTTCTATTCCAGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAACCGATGCGGt                                                                                                                   <  3:275565/149‑1 (MQ=255)
                                 aaCACTTCGGCCGAACCGAATGCCTTGGTCGCCTCGGAAATGACCCGTGGGAAGTTCTGTTCCAGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTc                                                                                                                  <  4:77064/149‑1 (MQ=255)
                                 aaCACTTCGGCCGAACCGAATGCCTTGGTCGCCTCGGAAATGACCCGTGGGAAGTTCTGTTCCAGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTc                                                                                                                  <  4:119355/149‑1 (MQ=255)
                                             gaaccgaaTGCCTTGGTCGCCTCGGAAATGACCCGTGGGAAGTTCTGTTCCAGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTCAg                                                                                                      >  2:163943/1‑149 (MQ=255)
                                              aaccgaaTGCCTTGGTCGCCTCGGAAATGACCCGTGGGAAGTTCTGTTCCAGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTCAgg                                                                                                     >  7:106990/1‑149 (MQ=255)
                                                        cTTGGTCGCCTCGGAAATGACCCGTGGGAAGTTCTGTTCCAGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAACCGAt                                                                                                                        >  2:366084/1‑120 (MQ=255)
                                                        cTTGGTCGCCTCGGAAATGACCCGTGGGAAGTTCTGTTCCAGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAACCGAt                                                                                                                        <  1:366084/120‑1 (MQ=255)
                                                        cTTGGTCGCCTCGGAAATGACCCGTGGGAAGTTCTGTTCCAGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTCAGGGCTTCATGaa                                                                                           >  1:349145/1‑149 (MQ=255)
                                                         ttGGTCGCCTCGGAAATGACCCGTGGGAAGTTCTGTTCCAGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGTCCTTGAGCATCACCGGATAACCGATGCGGTc                                                                                                                  >  6:31319/1‑125 (MQ=255)
                                                         ttGGTCGCCTCGGAAATGACCCGTGGGAAGTTCTGTTCCAGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTc                                                                                                                  <  5:31319/125‑1 (MQ=255)
                                                            gTCGCCTCGGAAATGACCCGTGGGAAGTTCTGTTCCAGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTCAGGGCTTCATGAATATc                                                                                       <  3:297397/149‑1 (MQ=255)
                                                               gCCTCGGAAATGACCCGTGGGAAGTTCTGTTCCAGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTCAGGGCTTCATGAATATCgg                                                                                     <  2:109870/148‑1 (MQ=255)
                                                               gCCTCGGAAATGACCCGTGGGAAGTTCTGTTCCAGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTCAGGGCTTCATGAATATCgg                                                                                     >  2:266090/1‑148 (MQ=255)
                                                               gCCTCGGAAATGACCCGTGGGAAGTTCTGTTCCAGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAAACGATGCGGTCACCTTCGCTCAGGGCTTCATGAATATCgg                                                                                     >  1:343683/1‑148 (MQ=255)
                                                                ccTCGGAAATGACCCGTGGGAAGTTCTGTTCCAGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTCAGGGCTTCATGAATATCGGCa                                                                                   >  8:22452/1‑149 (MQ=255)
                                                                 cTCGGAAATGACCCGTGGGAAGTTCTGTTCCAGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTCAGGGCTTCATGAATATCGGCaa                                                                                  <  7:76387/149‑1 (MQ=255)
                                                                               gTGGGAAGTTCTGTTCCAGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTCAGGGCTTCATGAATATCGGCAACGTTACCTTCGGTg                                                                    >  8:218417/1‑149 (MQ=255)
                                                                                    aaGTTCTGTTCCAGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTCAGGGCTTCATGAATATCGGCAACGTTACCTTCGGTGCCCgg                                                               <  8:259787/149‑1 (MQ=255)
                                                                                     aGTTCTGTTCCAGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTCAGGGCTTCATGAATATCGGCAACGTTACCTTCGGTGCCCggg                                                              <  6:5732/149‑1 (MQ=255)
                                                                                      gTTCTGTTCCAGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTCAGGGCTTCATGAATATCGGCAACGTTACCTTCGGTGCCCgggg                                                             <  2:37851/149‑1 (MQ=255)
                                                                                            ttCCAGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTCAGGGCTTCATGAATATCGGCAACGTTACCTTCGGTGCCCGGGGTTAcc                                                        <  5:7823/148‑1 (MQ=255)
                                                                                            ttCCAGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTCAGGGCTTCATGAATATCGGCAACGTTACCTTCGGTGCCCGGGGTTACCg                                                       >  6:46025/1‑149 (MQ=255)
                                                                                             tCCAGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTCAGGGCTTCATGAATATCGGCAACG‑TACCTTCGGTGCCCGGGGTTACCGGc                                                     >  1:115665/1‑149 (MQ=255)
                                                                                              ccAGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTCAGGGCTTCATGAATATCgg                                                                                     <  6:2980/117‑1 (MQ=255)
                                                                                              ccAGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTCAGGGCTTCATGAATATCgg                                                                                     >  5:2980/1‑117 (MQ=255)
                                                                                                aGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTCAGGGCTTCATGAATATCGGCaa                                                                                  <  6:147941/118‑1 (MQ=255)
                                                                                                     ttcGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTCAGGGCTTCATGAATATCGGCAACGTTACCTTCGGTGCCCGGGGTTACCGGCACACcgg                                              >  7:34899/1‑149 (MQ=255)
                                                                                                        gcgGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTCAGGGCTTCATGAATATCGGCAACGTTACCTTCGGTGCCCGGGGTTACCGGCACACcggcgg                                           >  8:264359/1‑149 (MQ=255)
                                                                                                                 gCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTCAGGGCTTCATGAATATCGGCAACGTTACCTTCGGTGCCCGGGGTTACCGGCACACCGGCGGCGATCATg                                   <  5:342964/148‑1 (MQ=255)
                                                                                                                  cAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTCAGGGCTTCATg                                                                                             >  3:11383/1‑89 (MQ=255)
                                                                                                                  cAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTCAGGGCTTCATg                                                                                             <  4:11383/89‑1 (MQ=255)
                                                                                                                   aaCGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTCAGGGCTTCATGAATATCGGCAACGTTACCTTCGGTGCCCGGGGTTACCGGCACACCGGCGGCGATCATGGTg                                >  1:168610/1‑149 (MQ=255)
                                                                                                                   aaCGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTCAGGGCTTCATGAATATCGGCAACGTTACCTTCGGTGCCCGGGGTTACCGGCACACCGGCGGCGATCATGGTg                                >  4:333517/1‑149 (MQ=255)
                                                                                                                     cGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTCAGGGCTTCATGAATATCGGCACCGTTACCTTCGGTGCCCGGGGTTACCGGCACACCGGCGGCGATCATGGTgcg                              >  3:278634/1‑149 (MQ=255)
                                                                                                                         gAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTCAGGGCTTCATGAATATCGGCAACGTTACCTTCGGTGCCCGGGGTTACCGGCACACCGGCGGCGATCATGGTGCGgcgc                          >  7:99002/1‑149 (MQ=255)
                                                                                                                           aTACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTCAGGGCTTCATGAATATCGGCAACGTTACCTTCGGTGCCCGGGGTTACCGGCACACCGGCGGCGATCATGGTGCGgcgcgc                        <  1:163943/149‑1 (MQ=255)
                                                                                                                             aCCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTCAGGGCTTCATGAATATCGGCAACGTTACCTTCGGTGCCCGGGGTTACCGGCACACCGGCGGCGATCATGGTGCGGCGCGCtt                      >  1:369857/1‑149 (MQ=255)
                                                                                                                               cACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTCAGGGCTTCATGAATATCGGCAACGTTACCTTCGGTGCCCGGGGTTACCGGCACACCGGCGGCGATCATGGTGCGGCGCGCTTCg                    >  1:349369/1‑149 (MQ=255)
                                                                                                                                aCGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTCAGGGCTTCATGAATATCGGCAACGTTACCTTCGGTGCCCGGGGTTACCGGCACACCGGCGGCGATCATGGTGCGGCGCGCTTCgg                   >  2:63416/1‑149 (MQ=255)
                                                                                                                                 tcaaggccACCTCCGGTGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTTAGGGCTTCATGAATATCGGCAACGTTACCTTCGGTGCCCGGGGTTACCGGCACACCGGCGGCGATCATGGTGCGGCGCGCtt                      >  8:112761/6‑145 (MQ=255)
                                                                                                                                 tcaaggccACCTCCGGTGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTTAGGGCTTCATGAATATCGGCAACGTTACCTTCGGTGCCCGGGGTTACCGGCACACCGGCGGCGATCATGGTGCGGCGCGCtt                      <  7:112761/140‑1 (MQ=255)
                                                                                                                                 tcaaggccACCTCCGGTGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTCAGGGCTTCATGAATATCGGCAACGTTACCTTCGGTGCCCGGGGTTACCGGCACACCGGCGGCGATCATGGTGCGGCGCGCTTCGGt                  >  7:225807/6‑149 (MQ=255)
                                                                                                                                  ggCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTCAGGGCTTCATGAATATCGGCAACGTTACCTTCGGTGCCCGGGGTTACCGGCACACCGGCGGCGATCATGGTGCGGCGCGCTTCGGTc                 <  8:34899/149‑1 (MQ=255)
                                                                                                                                   aaggccACCTCCGGTGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTCAGGGCTTCATGAATATGGGCAACGTTACCTTCGGTGCCCGGGGTTACCGGCACACcggc                                             >  1:119812/4‑120 (MQ=255)
                                                                                                                                   aaggccACCTCCGGTGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTCAGGGCTTCATGAATATCGGCAACGTTACCTTCGGTGCCCGGGGTTACCGGCACACcggc                                             <  2:119812/117‑1 (MQ=255)
                                                                                                                                    ccgccACCACCGGAGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTCAGGGCTTCATGAATATCGGCAACGTTACCTTCGGTGCCCGGGGTTACCGGCACACCGGCGGCGATCATGGTGCGGCGCGCTTCGGTCtt               <  2:369857/149‑1 (MQ=255)
                                                                                                                                          ccaccGGAGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTCAGGGCTTCATGAATATCGGCAACGTTACCTTCGGTGCCCGGGGTTACCGGCACACCGGCGGCGATCATGGTGCGGCGCGCTTCg                    >  4:37626/1‑138 (MQ=255)
                                                                                                                                          ccaccGGAGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTCAGGGCTTCATGAATATCGGCAACGTTACCTTCGGTGCCCGGGGTTACCGGCACACCGGCGGCGATCATGGTGCGGCGCGCTTCg                    <  3:37626/138‑1 (MQ=255)
                                                                                                                                          ccaccGGAGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTCAGGGCTTCATGAATATCGGCAACGTTACCTTCGGTGCCCGGGGTTACCGGCACACCGGCGGCGATCATGGTGCGGCGCGCTTCGGTCTTGTCGcc         <  7:264359/149‑1 (MQ=255)
                                                                                                                                            accGGAGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTCAGGGCTTCATGAATATCGGCAACGTTACCTTCGGTGCCCGGGGTTACCGGCACACCGGCGGCGATCATGGTGCGGCGCGCTTCGGTCTTGTCGCCCa       >  6:264807/1‑149 (MQ=255)
                                                                                                                                                gAGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTCAGGGCTTCATGAATATCGGCAACGTTACCTTCGGTGCCCGGGGTTACCGGCACACCGGCGGCGATCATGGTGCGGCGCGCTTCGGTCTTGTCGCCCATACg   <  2:122741/149‑1 (MQ=255)
                                                                                                                                                 aGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTCAGGGCTTCATGAATATCGGCAACGTTACCTTCGGTGCCCGGGGTTACCGGCACACCGGCGGCGATCATGGTGCGGCGCGCTTCGGTCTTGTCGCCCATACgg  >  6:205249/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
ATGTGCTTAGGGTTGACGATGCATTTCTCCAGGAACACTTCGGCCGAACCGAATGCCTTGGTCGCCTCGGAAATGACCCGTGGGAAGTTCTGTTCCAGTTCTTCGCGGCTATTGCAACGGCGAATACCACGGCCGCCACCACCGGAGGTGGCCTTGAGCATCACCGGATAACCGATGCGGTCACCTTCGCTCAGGGCTTCATGAATATCGGCAACGTTACCTTCGGTGCCCGGGGTTACCGGCACACCGGCGGCGATCATGGTGCGGCGCGCTTCGGTCTTGTCGCCCATACGG  >  NC_002947/6096075‑6096368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑