Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,103,973	A→T	5.4%	Q269L (CAA→CTA) ‡	PP_5353 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,103,973	0	A	T	5.4%	285.9 / 4.6	92	Q269L (CAA→CTA) ‡	PP_5353	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (41/46);  new base T (3/2);  total (44/48)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.75e-01

ATCGATAACCTGGAAGTGCAACTGGACGGTGTCGATGACCGCGCCGACCGCACCGACGCTACCCTGACCTTCCGTGGCGTGTCGAAGAAT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGT  >  NC_002947/6103828‑6104120                                                                                                                                                    |                                                                                                                                                    aTCGATAACCTGGAAGTGCAACTGGACGGTGTCGATGACCGCGCCGACCGCACCGACGCTACCCTGACCTTCCGTGGCGTGTCGAAGAAT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCaatg                                                                                                                                                  <  2:250075/149‑3 (MQ=255) aTCGATAACCTGGAAGTGCAACTGGACGGTGTCGATGACCGCGCCGACCGCACCGACGCTACCCTGACCTTCCGTGGCGTGTCGAAGAAT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAgg                                                                                                                                                  >  5:249035/1‑149 (MQ=255)  tCGATAACCTGGAAGTGCAACTGGACGGTGTCGATGACCGCGCCGACCGCACCGACGCTACCCTGACCTTCCGTGGCGTGTCGAAGAATAACGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAgg                                                                                                                                                  <  1:298302/149‑1 (MQ=255)    gATAACCTGGAAGTGCAACTGGACGGTGTCGATGACCGCGCCGACCGCACCGACGCTACCCTGACCTTCCGTGGCGTGTCGAAGAAT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCga                                                                                                                                               <  4:59204/149‑1 (MQ=255)    gATAACCTGGAAGTGCAACTGGACGGTGTCGATGACCGCGCCGACCGCACCGACGCTACCCTGACCTTCCGTGGCGTGTCGAAGAAT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGCTCGCGCCCAAGGCga                                                                                                                                               <  7:4900/149‑1 (MQ=255)         ccTGGAAGTGCAACTGGACGGTGTCGATGACCGCGCCGACCGCACCGACGCTACCCTGACCTTCCGTGGCGTGTCGAAGAAT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAAc                                                                                                                                           >  4:59887/1‑148 (MQ=255)          cTGGAAGTGCAACTGGACGGTGTCGATGACCGCGCCGACCGCACCGACGCTACCCTGACCTTCCGTGGCGTGTCGAAGAAT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAAc                                                                                                                                           >  5:243608/1‑147 (MQ=255)                 tGCAACTGGACGGTGTCGATGACCGCGCCGACCGCACCGACGCTACCCTGACCTTCCGTGGCGTGTCGAAGAAT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCTCGCCTTGGGCGCGAACCatgtgcca                                                                                                                                  <  8:336343/149‑8 (MQ=255)                 tGCAACTGGACGGTGTCGATGACCGCGCCGACCGCACCGACGCTACCCTGACCTTCCGTGGCGTGTCGAAGAAT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTgg                                                                                                                                  <  4:241805/149‑1 (MQ=255)                  gCAACTGGACGGTGTCGATGACCGCGCCGACCGCACCGACGCTACCCTGACCTTCCGTGGCGTGTCGAAGAAT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCTCGCCTTGGGCGCGAACCatgtgcca                                                                                                                                  <  4:307558/148‑8 (MQ=255)                   cAACTGGACGGTGTCGATGACCGCGCCGACCGCACCGACGCTACCCTGACCTTCCGTGGCGTGTCGAAGAAT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGc                                                                                                                                 <  4:354260/148‑1 (MQ=255)                   cAAATGGACGGTGTCGATGACCGCGCCGACCGCACCGACGCTACCCTGACCTTCCGTGGCGTTTCGAAGATT‑TCGCGCTTCGACGAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATTGTTCGCGGCCAAGGCGAGAACCAGCCTTGGc                                                                                                                                 <  3:122498/148‑1 (MQ=255)                    aaCTGGACGGTGTCGATGACCGCGCCGACCGCACCGACGCTACCCTGACCTTCCGTGGCGTGTCGAAGAAT‑TCGCGCTGCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTg                                                                                                                               <  3:34012/149‑1 (MQ=255)                     aCTGGACGGTGTCGATGACCGCGCCGACCGCACCGACGCTACCCTGACCTTCCGTGGCGTGTCGAAGAAT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTgg                                                                                                                              <  6:249035/149‑1 (MQ=255)                     aCTGGACGGTGTCGATGACCGCGCCGACCGCACCGACGCTACCCTGACCTTCCGTGGCGTGTCGAAGAAT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTgg                                                                                                                              >  5:247633/1‑149 (MQ=255)                           cGGTGTCGATGACCGCGCCGACCGCACCGACGCTACCCTGACCTTCCGTGGCGTGTCGAAGAAT‑TCTCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCGCAAGTCGAGAACCAGCCTTGGCTGGTAGCCg                                                                                                                        <  8:149030/149‑1 (MQ=255)                           cGGTGTCGATGACCGCGCCGACCGCACCGACGCTACCCTGACCTTCCGTGGCGTGTCGAAGAAT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCg                                                                                                                        >  3:181409/1‑149 (MQ=255)                            ggTGTCGATGACCGCGCCGACCGCACCGACGCTACCCTGACCTTCCGTGGCGTGTCGAAGAAT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCgg                                                                                                                       <  5:251697/149‑1 (MQ=255)                                tCGATGACCGCGCCGAGCGGACCGACGCTACCCTGACCTTCCGGGGCGTGTCGAAGAAT‑TCGCGCTTCGACCAGGGCTAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCTCCCAAGGCGAGAACCAGCGTTGGCTGGTAGCCGGTAt                                                                                                                    <  4:324506/148‑1 (MQ=255)                                  gATGACCGCGCCGACCGCACCGACGCTACCCTGACCTTCCGTGGCGTGTCGAAGAAT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTgg                                                                                                                              <  2:301422/136‑1 (MQ=255)                                  gATGACCGCGCCGACCGCACCGACGCTACCCTGACCTTCCGTGGCGTGTCGAAGAAT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTgg                                                                                                                              >  1:301422/1‑136 (MQ=255)                                  gATGACCGCGCCGACCGCACCGACGCTACCCTGACCTTCCGTGGCGTGTCGAAGAAT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCg                                                                                                                 >  4:185334/1‑149 (MQ=255)                                       ccGCGCCGACCGCACCGACGCTACCCTGACCTTCCGTGGCGTGTCGAAGAAT‑TCGCGCTTCGACCACGGCGAA‑GTGTTCAGCGAAACCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCAGTCaa                                                                                                             >  6:174235/1‑148 (MQ=255)                                           gcCGACCGCACCGACGCTACCCTGACCTTCCGTGGCGTGTCGAAGAAT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAg                                                                                                        <  3:132764/149‑1 (MQ=255)                                           gcCCACCGCACCTAACGTACCCTGTCCTTACGTGGGGTTTCGAATAAT‑TGGGGGTTTGCTCATGGGGAATGTCTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACa                                                                                                         <  1:117811/149‑1 (MQ=255)                                                   cACCGACGCTACCCTGACCTTCCGTGGCGTGTCGAAGAAT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGc                                                                                                >  7:127943/1‑149 (MQ=255)                                                   cACCGACGCTACCCTGACCTTCCGTGGCGTGTCGAAGAAT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGc                                                                                                >  3:296732/1‑149 (MQ=255)                                                      cGACGCTACCCTGACCTTCCGTGGCGTGTCGAAGAAT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGc                                                                                             >  5:328896/1‑149 (MQ=255)                                                      cGACGCTACCCTGACCTTCCGTGGCGTGTCGAAGAAT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGc                                                                                             >  5:133575/1‑149 (MQ=255)                                                         cGCTACCCTGACCTTCCGTGGCGTGTCGAAGAAT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTg                                                                                          >  5:70200/1‑149 (MQ=255)                                                              cccTGACCTTCCGTGGCGTGTCGAAGAAT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTaa                                                                                      <  4:287399/148‑1 (MQ=255)                                                              cccTGACCTTCCGTGGCGTGTCGAAGAAT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTaa                                                                                      <  2:346478/148‑1 (MQ=255)                                                               ccTGACCTTCCGTGGCGTGTCGAAGAAT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTaaa                                                                                     >  7:345296/1‑148 (MQ=255)                                                               ccTGACCTTCCGTGGCGTGTCGAAGAAT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTaaa                                                                                     >  2:166267/1‑148 (MQ=255)                                                                 tGACCTTCCGTGGCGTGTCGAAGAAT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCGTTGTCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGc                                                                                             <  3:351565/138‑1 (MQ=255)                                                                 tGACCTTCCGTGGCGTGTCGAAGAAT‑TCGCGCTTCGACAAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTCGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCgacaacgg                                                                                     >  4:351565/1‑139 (MQ=255)                                                                    ccTTCCGTGGCGTGTCGAAGAAT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAg                                                                                >  5:342965/1‑148 (MQ=255)                                                                    ccTTCCGTGGCGTGTCGAAGAAT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAg                                                                                >  7:346079/1‑148 (MQ=255)                                                                      ttCCGTGGCGTGTCGAAGAAT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAAc                                                                             >  3:63542/1‑149 (MQ=255)                                                                      ttCCGTGGCGTGTCGAAGAAT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAAc                                                                             >  4:276051/1‑149 (MQ=255)                                                                         cGTGGCGTGTCGAAGAAT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAAcccc                                                                          <  6:247633/149‑1 (MQ=255)                                                                             gCGTGTCGAAGAAT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAATCAGCTAATCGCTGCGTGCTAAACACAGAACCCCggg                                                                       <  6:243608/148‑1 (MQ=255)                                                                             gCGTGTCGAAGAAT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCggg                                                                       <  2:254878/148‑1 (MQ=255)                                                                             gCGTGTCGAAGAAT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCggg                                                                       <  8:132991/148‑1 (MQ=255)                                                                             gCGTGTCGAAGAAT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTCGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCACGGGc                                                                      >  6:264426/1‑149 (MQ=255)                                                                                  tCGAAGAAT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCg                                                                                                                        >  3:95013/1‑94 (MQ=255)                                                                                  tCGAAGAAT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCg                                                                                                                        <  4:95013/94‑1 (MQ=255)                                                                                  tCGAAGAAT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGcc                                                                 >  1:259659/1‑149 (MQ=255)                                                                                    gaagaaT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCg                                                               <  4:102833/149‑1 (MQ=255)                                                                                     aagaaT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCgg                                                              >  6:190046/1‑149 (MQ=255)                                                                                     aagaaT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCgg                                                              >  6:219508/1‑149 (MQ=255)                                                                                     aagaaT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCAGGTATCCGTCAAAACAGCTAATCGCAGCGTGCTAAACACAGAACCCCGGGCTTGCCCgg                                                              >  6:346944/1‑149 (MQ=255)                                                                                     aagaaT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCACCGAAAGCTGGCACATGGTAAGCGCCCAAGGGGAGAACCAGCCTTGGCTGGTAGCCGCTATCCGTCAAAACAGCTAATCGCTGCCTGCTAAACACAGACCCACGGGCTTGCCCgg                                                              >  4:31648/1‑149 (MQ=255)                                                                                      agaaT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCggg                                                             <  8:346079/149‑1 (MQ=255)                                                                                      agaaT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCggg                                                             >  1:144031/1‑149 (MQ=255)                                                                                      agaaT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCggg                                                             <  6:342965/149‑1 (MQ=255)                                                                                      agaaT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCAAGCCCggg                                                             <  4:296732/149‑1 (MQ=255)                                                                                       gaaT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCgggg                                                            >  2:95508/1‑149 (MQ=255)                                                                                        aaT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGt                                                           <  6:70200/149‑1 (MQ=255)                                                                                              gcgcTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAAAACCCCGGGCAAGCCCGGGGttctgt                                                      <  1:145511/149‑5 (MQ=255)                                                                                                 cTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTttttgctt                                                   >  5:161083/1‑149 (MQ=255)                                                                                                    cGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTttttgcttt                                                  <  5:190046/147‑1 (MQ=255)                                                                                                     gACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTttttgcttttg                                                <  4:181409/148‑1 (MQ=255)                                                                                                             cGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTATTCTTTTGCCAAAGTgg                                       >  7:7162/1‑149 (MQ=255)                                                                                                               aa‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAac                                                                                    >  5:94509/1‑102 (MQ=255)                                                                                                               aa‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAac                                                                                    <  6:94509/102‑1 (MQ=255)                                                                                                                    gtTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGctac                                  >  7:17414/1‑148 (MQ=255)                                                                                                                    gtTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGctac                                  >  5:213418/1‑148 (MQ=255)                                                                                                                     ttCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGctac                                  >  6:14685/1‑147 (MQ=255)                                                                                                                        aGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAggg                             <  3:276051/149‑1 (MQ=255)                                                                                                                        aGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAgg                              <  6:180329/148‑1 (MQ=255)                                                                                                                        aGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAgg                              <  8:345296/148‑1 (MQ=255)                                                                                                                        aGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAgg                              <  2:259659/148‑1 (MQ=255)                                                                                                                        aGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAgg                              <  5:219508/148‑1 (MQ=255)                                                                                                                            aaaGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAgg                              >  2:34280/1‑144 (MQ=255)                                                                                                                            aaaGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGCTTTTTGCTTTTGCCAAAGTGGGCTACTAgg                              <  1:34280/144‑1 (MQ=255)                                                                                                                              aGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAg                       <  5:310025/149‑1 (MQ=255)                                                                                                                               gCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAgg                      <  3:237052/149‑1 (MQ=255)                                                                                                                                cTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGgc                     >  8:160733/1‑149 (MQ=255)                                                                                                                                   aaggctGGTTCTCGCCTTGGGCGCGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGgcg                    >  2:372894/6‑147 (MQ=255)                                                                                                                                    aggctGGTTCTCGCCTTGGGCGCGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACACCGGGCTTGGCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAg                 >  5:242158/5‑149 (MQ=255)                                                                                                                                     acaTGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGc                >  2:127263/1‑149 (MQ=255)                                                                                                                                     acaTGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGc                <  8:17414/149‑1 (MQ=255)                                                                                                                                        tGGTTCTCGCCTTGGGCGCGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGtt             >  8:99709/1‑149 (MQ=255)                                                                                                                                          gTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGt           <  5:224881/149‑1 (MQ=255)                                                                                                                                          gTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGt           >  6:71724/1‑149 (MQ=255)                                                                                                                                           ttCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGt           >  7:128062/1‑148 (MQ=255)                                                                                                                                            tCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCTGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTc          <  6:328896/148‑1 (MQ=255)                                                                                                                                              gcgcCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATc       <  6:213418/149‑1 (MQ=255)                                                                                                                                                 cccAATGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAg    <  3:59887/149‑1 (MQ=255)                                                                                                                                                  ccAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATACGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCAGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAgg   >  5:298397/1‑149 (MQ=255)                                                                                                                                                   cAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGt  <  8:128062/149‑1 (MQ=255)                                                                                                                                                   cAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGt  <  1:166267/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    ATCGATAACCTGGAAGTGCAACTGGACGGTGTCGATGACCGCGCCGACCGCACCGACGCTACCCTGACCTTCCGTGGCGTGTCGAAGAAT‑TCGCGCTTCGACCAGGGCGAA‑GTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGT  >  NC_002947/6103828‑6104120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑