Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,143,642 A→G 9.1% T75A (ACC→GCC) ‡ cusF → copper efflux protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,143,6420AG 9.1% 208.7 / 9.8 77T75A (ACC→GCC) ‡cusFcopper efflux protein
Reads supporting (aligned to +/- strand):  ref base A (30/40);  new base G (4/3);  total (34/43)
Fisher's exact test for biased strand distribution p-value = 6.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.30e-01

GGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAGGTGGGAGACAACGTAGAGTTTGATTTCCGGATGGATGGCAGCACGGCAACGATTGTTGATATTCGCAAACAGTAATTCGCCTAATTATCCTGTCTGCCAA  >  NC_002947/6143495‑6143786
                                                                                                                                                   |                                                                                                                                                
gggATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGAc                                                                                                                                                 <  7:305600/149‑1 (MQ=255)
   aTGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCAt                                                                                                                                              <  8:199132/149‑1 (MQ=255)
       gCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGcc                                                                                                                                          <  6:349669/149‑1 (MQ=255)
         atggatggAATGGATATGAAGGAAACCCAATCAGCACCTTCCGCTCTTGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGACGGCGATGACCATGGCCtt                                                                                                                                        >  4:197916/1‑149 (MQ=255)
         atggatggAATGGATATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGGTCATGGccgg                                                                                                                                        <  1:303619/149‑3 (MQ=255)
          tggatggaATGGAAATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGGTCATGGccggc                                                                                                                                       <  5:122889/149‑4 (MQ=255)
              tggaatggaTATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCaaa                                                                                                                                    <  2:75395/148‑1 (MQ=255)
              tggaatggaTATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAg                                                                                                                                   >  2:297568/1‑149 (MQ=255)
                 aatggaTATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCct                                                                                                                                >  6:349561/1‑149 (MQ=255)
                   tggaTATGAAGGAAACCCAATCAGCACGTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCctct                                                                                                                              <  1:229599/149‑1 (MQ=255)
                   tggaTATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCAGGCCATGGTCATGGccggccat                                                                                                                                    <  1:266836/143‑7 (MQ=255)
                   tggaTATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCAGGCCATGGTCATGGccggccat                                                                                                                                    >  2:266836/1‑137 (MQ=255)
                        atGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTgcgc                                                                                                                          >  4:335211/1‑148 (MQ=255)
                         tGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCaa                                                                                                                        <  3:137317/149‑1 (MQ=255)
                          gAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCaa                                                                                                                        >  1:286563/1‑148 (MQ=255)
                          gAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCaa                                                                                                                        >  4:313130/1‑148 (MQ=255)
                          gAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCaa                                                                                                                        >  6:253434/1‑148 (MQ=255)
                             ggAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACGGGTTGCTGCGTTGAAAAGGCCGGCCAAGACCATGGCCTAGAACGCCTCTGCGCAACAGc                                                                                                                    >  8:317835/1‑149 (MQ=255)
                               aaaCCCAATCAGCACGTTCCGCTGATGCAGAAGGGACTGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCGTCAAAGCCTCTGCGCAACAGCTc                                                                                                                  <  5:101016/149‑1 (MQ=255)
                               aaaCCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCATTTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTc                                                                                                                  <  3:261040/149‑1 (MQ=255)
                                aaCCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCg                                                                                                                 <  5:349561/149‑1 (MQ=255)
                                 aCCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGa                                                                                                                >  3:139936/1‑149 (MQ=255)
                                            aCCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGTATTGAAgg                                                                                                     <  6:327666/149‑1 (MQ=255)
                                              cTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAGGTg                                                                                                   >  8:175477/1‑149 (MQ=255)
                                               ttCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAGGTgg                                                                                                  >  3:288171/1‑149 (MQ=255)
                                               ttCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAGGTgg                                                                                                  >  8:331279/1‑149 (MQ=255)
                                                 cctcTCATGCAGAAGGGACTGTAATGGCAATTGACGCCCAAGGCGTCACAGTTACCCTGATGAATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATTGATTGAAGGTgg                                                                                                  <  3:205589/144‑1 (MQ=255)
                                                 ccGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAGGTgg                                                                                                  <  5:367663/147‑1 (MQ=255)
                                                 ccGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAGGTgg                                                                                                  <  7:331279/147‑1 (MQ=255)
                                                  cGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAGGTGGga                                                                                                <  5:253434/148‑1 (MQ=255)
                                                  cGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAGGTGGga                                                                                                <  7:175477/148‑1 (MQ=255)
                                                  cGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAGGTGGga                                                                                                <  4:139936/148‑1 (MQ=255)
                                                          cggAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAGGTGGGAGACAACGTa                                                                                       <  6:224745/147‑1 (MQ=255)
                                                          cAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGTACCGTTTGCTGCGTTGAAATTGCCGGCCATGACCATGGCCTTCAAATCCTCTGCGCAACAGCTCGATGGATTGAAGGTGGGAGACAACg                                                                                         >  8:68383/1‑147 (MQ=255)
                                                          cAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAGGTGGGAGACAACGTa                                                                                       <  3:98877/149‑1 (MQ=255)
                                                          cAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAGGTGGGAGACAACGTa                                                                                       <  2:286563/149‑1 (MQ=255)
                                                             aaGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAGGTGGGAGACAACGTagag                                                                                    <  1:36262/149‑1 (MQ=255)
                                                               gggACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAGGTGGGAGACAACGTAGAGtt                                                                                  >  2:195802/1‑149 (MQ=255)
                                                               gggACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAGGTGGGAGACAACGTAGAGtt                                                                                  <  2:94467/149‑1 (MQ=255)
                                                                 gACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAg                                                                                                      >  4:111550/1‑127 (MQ=255)
                                                                 gACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAg                                                                                                      <  3:111550/127‑1 (MQ=255)
                                                                 gACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAGGTGGGAGACAACGTAGAGTTTg                                                                                <  2:238752/149‑1 (MQ=255)
                                                                    ggTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGAt                                                                                                           <  4:95696/119‑1 (MQ=255)
                                                                    ggTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGAt                                                                                                           >  3:95696/1‑119 (MQ=255)
                                                                        aaGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAGGTGGGAGACAACGTAGAGTTTGATTTcc                                                                          <  6:12117/148‑1 (MQ=255)
                                                                          ggCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGAt                                                                                                           >  6:59653/1‑113 (MQ=255)
                                                                          ggCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGAt                                                                                                           <  5:59653/113‑1 (MQ=255)
                                                                                  aCGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAGGTGGGAGACAACGTAGAGTTTGATTTCCGGatggatgg                                                                >  2:275834/1‑148 (MQ=255)
                                                                                    gCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAGGTGGGAGACAACGTAGAGTTTGATTTCCGGATGGATGgcag                                                             >  6:129507/1‑149 (MQ=255)
                                                                                     cccAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAGGTGGGAGACAACGTAGAGTTTGATTTCCGGATGGATGgcagc                                                            <  5:129507/149‑1 (MQ=255)
                                                                                              gcACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAGGTGGGAGACAACGTAGAGTTTGATTTCCggatgga                                                                   >  4:17569/1‑133 (MQ=255)
                                                                                              gcACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAGGTGGGAGACAACGTAGAGTTTGATTTCCggatgga                                                                   <  3:17569/133‑1 (MQ=255)
                                                                                                  aGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAGGTGGGAGACAACGTAGAGTTTGATTTCCGGATGGATGGCAGCACGGCAACGAttg                                               <  1:195802/149‑1 (MQ=255)
                                                                                                      aCCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAGGTGGGAGACAACGTAGAGTTTGATTTCCGGATGGATGGCAGCACGGCAACGATTGTTGa                                           <  3:335211/149‑1 (MQ=255)
                                                                                                       cccTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAGGTGGGAGACAACGTAGAGTTTGATTTCCGGATGGATGGCAGCACGGCAACGATTGTTGat                                          <  1:275834/149‑1 (MQ=255)
                                                                                                        ccTGATGCATGGACCGGTTGCTGCGTTGATATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAGGt                                                                                                    <  5:318569/90‑1 (MQ=255)
                                                                                                        ccTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAGGt                                                                                                    >  6:318569/1‑90 (MQ=255)
                                                                                                            aTGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAGGTGGGAGACAACGTaga                                                                                     <  8:315966/101‑1 (MQ=255)
                                                                                                            aTGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAGGTGGGAGACAACGTaga                                                                                     >  7:315966/1‑101 (MQ=255)
                                                                                                            aTGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAGGTGGGAGACAACGTAGAGTTTGATTTCCGGATGGATGGCAGCACGGCAACGATTGTTGATATTc                                      <  4:288171/148‑1 (MQ=255)
                                                                                                                aTGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAGGTGGGAGACAACGTAGAGTTTGATTTCCGGATGGATGGCAGCACGGCAACGAttgttg                                            <  6:221280/138‑1 (MQ=255)
                                                                                                                aTGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAGGTGGGAGACAACGTAGAGTTTGATTTCCGGATGGATGGCAGCACGGCAACGAttgttg                                            >  5:221280/1‑138 (MQ=255)
                                                                                                                            ctgcGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAGGTGGGAGACAACGTAGAGTTTGATTTCCGGATGGATGGCAGCACGGCAACGATTGTTGATATTCGCAAACAGTAATTCGcc                     >  4:131029/1‑149 (MQ=255)
                                                                                                                            ctgcGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAGGTGGGAGACAACGTAGAGTTTGATTTCCGGATGGATGGCAGCACGGCAACGATTGTTGATATTCGCAAACAGTAATTCGc                      <  8:284645/148‑1 (MQ=255)
                                                                                                                             tgcGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAGGTGGGAGACAACGTAGAGTTTGATTTCCGGATGGATGGCAGCACGGCAACGATTGTTGATATTCGCAAACAGTAATTCGcc                     >  6:377364/1‑148 (MQ=255)
                                                                                                                               cGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAGGTGGGAGACAACGTAGAGTTTGATTTCCGGATGGATGGCAGCACGGCAACGATTGTTGATATTCGCAAACAGTAATTCGCCTaa                  >  1:228616/1‑149 (MQ=255)
                                                                                                                                 ttGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCACGATGGATAGAAGGTGGGAGACAACGTAGAGTTTGATTTCCGGATGGATGGCAGCACGGCAACGATTGATGATATTCGCAAACAGTAAATCGCCTaaa                 >  5:123302/1‑147 (MQ=255)
                                                                                                                                     ctttgaaggccATGGTCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAGGTGGGAGACAACGTAGAGTTTGATTTCCGGATGGATGGCAGCACGGCAACGATTGTTGATATTCGCAAACAGTAATTCGCCTAATTATcc            >  6:22329/8‑149 (MQ=255)
                                                                                                                                      tttgaaggccATGGTCATGGCCTTCAAAGCCTCTGCGCGACAGCTCGATGGATTGAAGGTGGGAGACAACGTAGAGTGTGATTTCCGGATGGATGGCAGGACGGCAACGAGTGTTGATATTCGGAAGCAGTAGTTCGCGTAAGTAGccg           >  4:277449/7‑148 (MQ=255)
                                                                                                                                       ttgaaggccATGGTCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAGGTGGGAGACAACGTAGAGTTTGATTTCCGGATGGATGGCAGCACGGCAACGATTGTTGATATTCGCAAACAGTAATTCGCCTAATTATCCTg          >  1:163914/6‑149 (MQ=255)
                                                                                                                                         gccggccATGACCATGGCCTTCAAATCCTCTGCGCAACAGCTCGATGGATTGAAGGTTGGAGACAACGTATAGTTTGATTTCCGGATGGATGGCAGCACGGCAACGATTGTTGATATTCGCAAACAGTAATTCGCCTAATTATCCTGt         <  7:68383/148‑1 (MQ=255)
                                                                                                                                          ccggccATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAGGTGGGAGACAACGTAGAGTTTGATTTCCGGATGGATGGCAGCACGGCAACGATTGTTGATATTCGCAAACAGTAATTCGCCTAATTATCCTGTCt       <  6:43114/149‑1 (MQ=255)
                                                                                                                                           cggccATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAGGTGGGAGACAACGTAGAGTTTGATTTCCGGATGGATGGCAGCACGGCAACGATTGTTGATATTCGCAAACAGTAATTCGCCTAATTATCCTGTCTg      >  3:198691/1‑149 (MQ=255)
                                                                                                                                            ggccATGACCATTGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAGGTGGGAGACAACGTAGAGTTTGATTTCCGGATGGATGGCAGCACGGCAACGATTGTTGATATTCGCAAACAGTAATTCGCCTAATTATCCTGTCTGc     <  3:197916/149‑1 (MQ=255)
                                                                                                                                               cATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAGGTGGGAGACAACGTAGAGTTTGATTTCCGGATGGATGGCAGCACGGCAACGATTGTTGATATTCGCAAACAGTAATTCGCCTAATTATCCTGTCTGCCaa  >  1:271106/1‑149 (MQ=255)
                                                                                                                                                 tGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAGGTGGGAGACAACGTAGAGTTTGATTTCCGGATGGATGGCAGCACGGCAACGAttgt                                              <  6:191861/103‑1 (MQ=255)
                                                                                                                                                 tGACCATGGCCTTAAAAGCCTCTGCGCAACAGCTCGATGGATTGAAGGTGGGAGACAACGTAGAGTTTGATTTCCGGATGGATGGCAGCACGGCAACGAttgt                                              >  5:191861/1‑103 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAATGGCCGGCCATGACCATGGCCTTCAAAGCCTCTGCGCAACAGCTCGATGGATTGAAGGTGGGAGACAACGTAGAGTTTGATTTCCGGATGGATGGCAGCACGGCAACGATTGTTGATATTCGCAAACAGTAATTCGCCTAATTATCCTGTCTGCCAA  >  NC_002947/6143495‑6143786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑