Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,155,806 A→G 7.8% S121P (TCG→CCG)  PP_5399 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,155,8060AG 7.8% 143.1 / 2.6 51S121P (TCG→CCG) PP_5399hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (20/27);  new base G (2/2);  total (22/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

AGTTCGCATTTTTTACGACAGCAGTAACCTGCGAAGCACCTAGCGGCTTATCACTAGAGAAATTTCGAAGGTGGGCTGCAGTCACCTCTATCTCTGTTCCACCGCCCAGCCGCTGATCCGACCTGCAGACCAGGGCGTAGTGGCTCGATTTGCGCCGTCCTGAAGGAAGATCTGCTCGGCTGGTGATCAAGACGTGTTTGGGGAGAGGCACAACGTTACCTCGCCCATCGACCCAACTGTTCCAGACAAAGATGTCTTCAGGGCGAACGTCAATGTCCTTCGGCTTGCTGAACATC  >  NC_002947/6155659‑6155954
                                                                                                                                                   |                                                                                                                                                    
aGTTCGCATTTTTTACGACAGCAGTAACCTGCGAAGCACCTAGCGGCTTATCACTAGAGAAATTTCGAAGGTGGGCTGCAGTCACCTCTATCTCTGTTCCACCGCCCAGCCGCTGATCCGACCTGCAGACCAGGGCGTAGTGGCTCGAt                                                                                                                                                     <  3:73502/149‑1 (MQ=255)
 gTTCGCATTTTTTACGACAGCAGTAACCTGCGAAGCACCTAGCGGCTTATCACTAGAGAAATTTCGAAGGTGGGCTGCAGTCACCTCTATCTCTGTTCCACCGCCCAGCCGCTGATCCGACCTGCAGACCAGGGCGTAGTGGCTCGAtt                                                                                                                                                    <  4:294472/149‑1 (MQ=255)
  ttCGCATTTTTTACGACAGCAGTAACCTGCGAAGCACCTAGCGGCTTATCACTAGAGAAATTTCGAAGGTGGGCTGCAGTCACCTCTATCTCTGTTCCACCGCCCAGCCGCTGATCCGACCTGCAGACCAGGGCGTAGTGGCTCGGttt                                                                                                                                                   >  7:269901/1‑149 (MQ=255)
  ttCGCATTTTTTACGACAGCAGTAACCTGCGAAGCACCTAGCGGCTTATCACTAGAGAAATTTCGAAGGTGGGCTGCAGTCACCTCTATCTCTGTTCCACCGCCCAGCCGCTGATCCGACCTGCAGACCAGGGCGTAGTGGCTCGGttt                                                                                                                                                   >  7:264803/1‑149 (MQ=255)
           tttACGACAGCAGTAACCTGCGAAGCACCTAGCGGCTTATCACTAGAGAAATTTCGAAGGTGGGCTGCAGTCACCTCTATCTCTGTTCCACCGCCCAGCCGCTGATCCGACCTGCAGACCAGGGCGTAGTGGCTCGATTTGCGCCGTc                                                                                                                                           <  8:42035/148‑1 (MQ=255)
                    gcagTAACCTGCGAAGCACCTAGCGGCTTATCACTAGAGAAATTTCGAAGGTGGGCTGCAGTCACCTCTATCTCTGTTCCACCGCCCAGCCGCTGATCCGACCTGCAGACCAGGGCGTAGTGGCTCGATTTGCGCCGTCCTgaaggaag                                                                                                                                 <  1:300695/149‑1 (MQ=255)
                      agTAACCTGCGAAGCACCTAGCGGCTTATCACTAGAGAAATTTCGAAGGTGGGCTGCAGTCACCTCTATCTCTGTTCCACCGCCCAGCCGCTGATCCGACCTGCAGACCAGGGCGTAGTGGCTCGATTTGCGCCGTCCTGAAGGAAGAt                                                                                                                               <  5:219147/149‑1 (MQ=255)
                          aCCTGCGAAGCACCTAGCGGCTTATCACTAGAGAAATTTCGAAGGTGGGCTGCAGTCACCTCTATCTCTGTTCCACCGCCCAGCCGCTGATCCGACCTGCAGACCAGGGCGTAGTGGCTCGATTTGCGCCGTCCTGAAGGAAGATCTg                                                                                                                            <  7:58818/148‑1 (MQ=255)
                            cTGCGAAGCACCTAGCGGCTTATCACTAGAGAAATTTCGAAGGTGGGCTGCAGTCACCTCTATCTCTGTTCCACCGCCCAGCCGCTGATCCGACCTGCAGACCAGGGCGTAGTGGCTCGATTTGCGCCGTCCTGAAGGAAGATCTGCTc                                                                                                                         >  3:172846/1‑149 (MQ=255)
                            cTGCGAAGCACCTAGCGGCTTATCACTAGAGAAATTTCGAAGGTGGGCTGCAGTCACCTCTATCTCTGTTCCACCGCCCAGCCGCTGATCCGACCTGCAGACCAGGGCGTAGTGGCTCGATTTGCGCCGTCCTGAAGGAAGATCTGCTc                                                                                                                         <  5:271556/149‑1 (MQ=255)
                                 aaGCACCTAGCGGCTTATCACTAGAGAAATTTCGAAGGTGGGCTGCAGTCACCTCTATCTCTGTTCCACCGCCCAGCCGCTGATCCGACCTGCAGACCAGGGCGTAGTGGCTCGATTTGCGCCGTCCTGAAGGAAGATCTGCTCGGCTg                                                                                                                    >  1:359240/1‑149 (MQ=255)
                                   gCACCTAGCGGCTTATCACTAGAGAAATTTCGAAGGTGGGCTGTAGTCACCTCTATCTCTGTTCCACCGCCCAGCCGCTGATCCGACCTGCAGACCAGGGCGTAGTGGCTCGATTTGCGCCGTCCTGAAGGAAGATCTGCTCGGCTGGt                                                                                                                  <  2:164479/149‑1 (MQ=255)
                                     aCCTAGCTGCTTATCACTAGAGAAATTTCGAAGGTGGGCTGCAGTCACCTCTATCTCTGTTCCACCGCCCAGCCGCTGATCCGACCTGCAGACCAGGGCGTAGTGGCTCGGTTTGCGCCGTCCTGAAGGAAGATCTGCTCGGCTGGTGa                                                                                                                <  8:264803/149‑1 (MQ=255)
                                     aCCTAGCGGCTTATCACTAGAGAAATTTCGAAGGTGGGCTGCAGTCACCTCTATCTCTGTTCCACCGCCCAGCCGCTGATCCGACCTGCAGACCAGGGCGTAGTGGCTCGGTTTGCGCCGTCCTGAAGGAAGATCTGCTCGGCTGGTGa                                                                                                                <  8:269901/149‑1 (MQ=255)
                                      ccTAGCGGCTTATCACTAGAGAAATTTCGAAGGTGGGCTGCAGTCACCTCTATCTCTGTTCCACCGCCCAGCCGCTGATCCGACCTGCAGACCAGGGCGTAGTGGCTCGATTTgc                                                                                                                                                 >  8:355028/1‑115 (MQ=255)
                                      ccTAGCGGCTTATCACTAGAGAAATTTCGAAGGTGGGCTGCAGTCACCTCTATCTCTGTTCCACCGCCCAGCCGCTGATCCGACCTGCAGACCAGGGCGTAGTGGCTCGATTTgc                                                                                                                                                 <  7:355028/115‑1 (MQ=255)
                                           cGGCTTATCACTAGAGAAATTTCGAAGGTGGGCTGCAGTCACCTCTATCTCTGTTCCACCGCCCAGCCGCTGATCCGACCTGCAGACCAGGGCGTAGTGGCTCGATTTGCGCCGTCCTGAAGGAAGATCTGCTCGGCTGGTGATCAAGa                                                                                                          <  2:344201/149‑1 (MQ=255)
                                            ggCTTATCACTAGAGAAATTTCGAAGGTGGGCTGCAGTCACCTCTATCTCTGTTCCACCGCCCAGCCGCTGATCCGACCTGCAGACCAGGGCGTAGTGGCTCGATTTGCGCCGTCCTGAAGGAAGAt                                                                                                                               <  5:41256/127‑1 (MQ=255)
                                            ggCTTATCACTAGAGAAATTTCGAAGGTGGGCTGCAGTCACCTCTATCTCTGTTCCACCGCCCAGCCGCTGATCCGACCTGCAGACCAGGGCGTAGTGGCTCGATTTGCGCCGTCCTGAAGGAAGAt                                                                                                                               >  6:41256/1‑127 (MQ=255)
                                                 aTCACTAGAGAAATTTCGAAGGTGGGCTGCAGTCACCTCTATCTCTGTTCCACCGCCCAGCCGCTGATCCGACCTGCAGACCAGGGCGTAGTGGCTCGATTTGCGCCGTCCTGAAGGAAGATCTGCTCGGCTGGTGATCAAGACgtgt                                                                                                     <  3:342211/148‑1 (MQ=255)
                                                    aCTAGAGAAATTTCGAAGGTGGGCTGCAGTCACCTCTATCTCTGTTCCACCGCCCAGCCGCTGATCCGACCTGCAGACCAGGGCGTAGTGGCTCGATTTGCGCCGTCCTGAAGGAAGATCTGCTCGGCTGGTGATCAAGACGTGTTTgg                                                                                                 <  6:122556/149‑1 (MQ=255)
                                                    aCTAGAGAAATTTCGAAGGTGGGCTGCAGTCACCTCTATCTCTGTTCCACCGCCCAGCCGCTGATCCGACCTGCAGACCAGGGCGTAGTGGCTCGATTTGCGCCGTCCTGAAGGAAGATCTGCTCGGCTGGTGATCAAGACGTGTTTgg                                                                                                 <  4:172846/149‑1 (MQ=255)
                                                    aCTAGAGAAATTTCGAAGGTGGGCTGCAGTCACCTCTATCTCTGTTCCACCGCCCAGCCGCTGATCCGACCTGCAGACCAGGGCGTAGTGGCTCGATTTGCGCCGTCCTGAAGGAAGATCTGCTCGGCTGGTGATCAAGACGTGTTTg                                                                                                  <  6:45789/148‑1 (MQ=255)
                                                      tAGAGAAATTTCGAAGGTGGGCTGCAGTCACCTCTATCTCTGTTCCTCCGCCCAGCCGCTGATCCGACCTGCAGACCAGGGCGTAGTGGCTCGATTTGCGCCGTCCTGAAGGAAGATCTg                                                                                                                            <  3:32559/120‑1 (MQ=255)
                                                      tAGAGAAATTTCGAAGGTCGGCTGCAGTCACCTCTATCTCTGTTCCACCGCCCAGCCGCTGATCCGACCTGCAGACAAGGGCCTAGTGGCACGATTTGCGCCCACCTGAAGGAAGATCTg                                                                                                                            >  4:32559/1‑120 (MQ=255)
                                                      tAGAGAAATTTCGAAGGGGGGCTGCAGTCACCTCTATCTCTGTTCCACCGCCCAGCCGCTGATCCGACCTGCAGACCAGGGCGTAGTGGCTCGATTTGCGCCGTCCTGAAGGAAGATCTGCTCGGCTGGTGATCAAGACGTGTTTggg                                                                                                >  7:140117/1‑148 (MQ=255)
                                                        gagaAATTTCGAATGTGGTCTGCAGTCTCCTCTATCTCTGTTGGACCGCCCAGGCGCTGATCCGACCTTCAGACCAGGGCGTTGTGTCTCGATTTGCGGGGTCCTGAAGGAAGATCTGCTCGGCTGGTGATCAAGACGTGTTTgggg                                                                                               <  5:125127/147‑1 (MQ=255)
                                                           aaaTTTCGAAGGTGGGCTGGAGTCACCTCTATCTCTGTTCCACCGCCCAGCCGCTGATCCGCCCTGCAGACCAGGGCGTAGTGGCTCGATTTGCGCCGTCCTGAATGAAGATCTGCTCGGCTGGTGATCAAGAAGTGTTTGGGGCGAgg                                                                                          >  3:237025/1‑149 (MQ=255)
                                                           aaaTTTCGAAGGTGGGCTGCAGTCACCTCTATCTCTGTTCCACCGCCCAGCCGCTGATCCGACCTGCAGACCAGGGCGTAGTGGCTCGATTTGCGCCGTCCTGAAGGAAGATCTGCTCGGCTGGTGATCAAGACGTGTTTGGGGAGAgg                                                                                          <  5:212018/149‑1 (MQ=255)
                                                                  gAAGGTGGGCTGCAGTCACCTCTATCTCTGTTCCACCGCCCAGCCGCTGATCCGACCTGCAGACCAGGGCGTAGTGGCTCGATTTGCGCCGTCCTGAAGGAAGATCTGCTCGGCTGGTGATCAAGACGTGTTTGGGGAGAGGCACAAc                                                                                    <  2:359240/148‑1 (MQ=255)
                                                                  gAAGGTGGGCTGCAGTCACCTCTATCTCTGTTCCACCGCCCAGCCGCTGATCCGACCTGCAGACCAGGGCGTAGTGGCTCGATTTGCGCCGTCCTGAAGGAAGATCTGCTCGGCTGGTGATCAAGACGTGTTTGGGGAGAGGCACAAc                                                                                    <  4:68402/148‑1 (MQ=255)
                                                                           cTGCAGTCACCTCTATCTCTGTTCCACCGCCCAGCCGCTGATCCGACCTGCAGACCAGGGCGTAGTGGCTCGATTTGCGCCGTCCTGAAGGAAGATCTGCTCGGCTGGTGATCAAGACGTGTTTGGGGAGAGGCACAACGTTACCTCGc                                                                          <  2:119807/149‑1 (MQ=255)
                                                                               aGTCACCTCTATCTCTGTTCCACCGCCCAGCCGCTGATCCGACCTGCAGACCAGGGCGTAGTGGCTCGATTTGCGCCGTCCTGAAGGAAGATCTGCTCGGCTGGTGATCAAGACGTGTTTGGGGAGAGGCACAACGTTACCTCGCCCAt                                                                      <  8:140117/149‑1 (MQ=255)
                                                                                   aCCTCTATCTCTGTTCCACCGCCCAGCCGCTGATCCGACCTGCAGACCAGGGCGTAGTGGCTCGATTTGCGCCGTCCTGAAGGAAGATCTGCTCGGCTGGTGATCAAGACGTGTTTGGGGAGAGGCACAACGTTACCTCGCCCATCGa                                                                   <  1:151283/148‑1 (MQ=255)
                                                                                     ctctATCTCTGTTCCACCGCCCAGCCGCTGATCCGACCTGCAGACCAGGGCGTAGTGGCTCGATTTGCGCCGTCCTGAAGGAAGATCTGCTCGGCTGGTGATCAAGACGTGTTTGGGGAGAGGCACAAc                                                                                    >  1:40560/1‑129 (MQ=255)
                                                                                     ctctATCTCTGTTCCACCGCCCAGCCGCTGATCCGACCTGCAGACCAGGGCGTAGTGGCTCGATTTGCGCCGTCCTGAAGGAAGATCTGCTCGGCTGGTGATCAAGACGTGTTTGGGGAGAGGCACAAc                                                                                    <  2:40560/129‑1 (MQ=255)
                                                                                      tctATCTCTGTTCCACCGCCCAGCCGCTGATCCGACCTGCAGACCAGGGCGTAGTGGCTCGATTTGCGCCGTCCTGAAGGAAGATCTGCTCGGCTGGTGATCAAGACGTGTTTGGGGAGAGGCACAACGTTACCTCGCCCATCGAccc                                                                >  3:7370/1‑148 (MQ=255)
                                                                                       ctATCTCTGTTCCACCGCCCAGCCGCTGATCCGACCTGCAGACCAGGGCGTAGTGGCTCGATTTGCGCCGTCCTGAAGGAAGATCTGCTCGGCTGGTGATCAAGACGTGTTTGGGGAGAGGAACAACGTTACCTCGCCGCTCGACCCaa                                                              >  3:239015/1‑149 (MQ=255)
                                                                                                ttCCACCGCCCAGCCGCTGATCCGACCTGCAGACCAGGGCGTAGTGGCTCGATTTGCGCCGTCCTGAAGGAAGATCTGCTCGGCTgg                                                                                                                   <  3:50466/87‑1 (MQ=255)
                                                                                                ttCCACCGCCCAGCCGCTGATCCGACCTGCAGACCAGGGCGTAGTGGCTCGATTTGCGCCGTCCTGAAGGAAGATCTGCTCGGCTgg                                                                                                                   >  4:50466/1‑87 (MQ=255)
                                                                                                    aCCGCCCAGCCGCTGATCCGACCTGCAGACCAGGGCGTAGTGGCTCGATTTGCGCCGTCCTGAAGGAAGATCTGCTCGGCTGGTGATCAAGACGTGTTTGGGGAGAGGCACAACGTTACCTCGCCCATCGACCCAACTGTTCCAGACa                                                  >  4:363526/1‑148 (MQ=255)
                                                                                                         ccAGCCGCTGATCCGACCTGCAGACCAGGGCGTAGTGGCTCGATTTGCGCCGTCCTGAAGGAAGATCTGCTCGGCTGGTGATCAAGACGTGTATGGGGAGAGGCACAACGTTACCTCGCCCATCGACCCAACTGTTc                                                        <  4:368405/137‑1 (MQ=255)
                                                                                                         ccAGCCGCTGATCCGACCTGCAGACCAGGGCGTAGTGGCTCGATTTGCGCCGTCCTGAAGGAAGATCTGCTCGGCTGGTGATCAAGACGTGTATGGGGAGAGGCACAACGTTACCTCGCCCATCGACCCAACTGTTc                                                        >  3:368405/1‑137 (MQ=255)
                                                                                                                 tGATCCGACCTGCAGACCAGGGCGTAGTGGCTCGATTTGCGCCGTCCTGAAGGAAGATCTGCTCGGCTGGTGATCAAGACGTGTTAGGGGAGAGGCACAACGTTACCTCGCCCATCGCCCCAACTGTTCCAGACAAAGATGTCTTCAgg                                    >  7:58303/1‑149 (MQ=255)
                                                                                                                     ccGACCTGCAGACCAGGGCGTAGTGGCTCGATTTGCGCCGTCCTGAAGGAAGATCTGCTCGGCTGGTGATCAAGACGTGTTTGGGGAGAGGCACAACGTTACCTCGCCCATCGACCCAACTGTTCCAGACAAAGATGTCTTCAGGGCg                                 >  6:105604/1‑148 (MQ=255)
                                                                                                                       gACCTGCAGACCAGGGCGTAGTGGCTCGATTTGCGCCGTCCTGAAGGAAGATCTGCTCGGCTGGTGATCAAGACGTGTTTGGGGAGAGGCACAACGTTACCTCGCCCATCGACCCAACTGTTCCAGACAAAGATGTCTTCAGGGCGAAc                              <  3:363526/149‑1 (MQ=255)
                                                                                                                           tGCAGACCAGGGCGTAGTGGCTCGATTTGCGCCGTCCTGAAGGAAGATCTGCTCGGCTGGTGATCAAGACGTGTTTGGGGAGAGGCACAACGTTACCTCGCCCATCGACCCAACTGTTCCAGACAAAGATGTCTTCAGGGCGAACGTc                           >  3:341791/1‑148 (MQ=255)
                                                                                                                            gCAGACCAGGGCGTAGTGGCTCGATTTGCGCCGTCCTGAAGGAAGATCTGCTCGGCTGGTGATCAAGACGTGTTTGGGGAGAGGCACAACGTTACCTCGCCCATCGACCCAACTGTTCCAGACAAAGATGTCTTCAGGGCGAACGTCaa                         >  3:261176/1‑149 (MQ=255)
                                                                                                                                         tAGTGGCTCGATTTGCGCCGTCCTGAAGGAAGATCTGCTCGGCTGGTGATCAAGACGTGTTTGGGGAGAGGCACAACGTTACCTCGCCCATCGACCCAACTGTTCCAGACAAAGATGTCTTCAGGGCGAACGTCAATGTCCTTCGGCtt            >  4:89146/1‑149 (MQ=255)
                                                                                                                                               cTCGATTTGCGCCGTCCTGAAGGAAGATCTGCTCGGCTGGTGATCAAGACGTGTTTGGGGAGAGGCACAACGTTACCTCGCCCATCGACCCAACTGTTCCAGACAAAGATGTCTTCAGGGCGAACGTCAATGTCCTTCGGCTTGCTGaa      >  8:69360/1‑149 (MQ=255)
                                                                                                                                                tCGATTTGCGCCGTCCTGAAGGAAGATCTGCTCGGCTGGTGATCAAGACGTGTTTGGGGAGAGGCACAACGTTACCTCGCCCATCGACCCAACTGTTCCAGACAAAGATGTCTTCAGGGCGAACGTCAATGTCCTTCGGCTTGCTGaa      >  1:309458/1‑148 (MQ=255)
                                                                                                                                                   aTTTGCGCCGTCCTGAAGGAAGATCTGCTCGGCTGGTGATCAAGACGTGTTTGGGGAGAGGCACAACGTTACCTCGCCCATCGACCCAACTGTTCCAGACAAAGATGTCTTCAGGGCGAACGTCAATGTCCTTCGGCTTGCTGAACATc  >  7:317704/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
AGTTCGCATTTTTTACGACAGCAGTAACCTGCGAAGCACCTAGCGGCTTATCACTAGAGAAATTTCGAAGGTGGGCTGCAGTCACCTCTATCTCTGTTCCACCGCCCAGCCGCTGATCCGACCTGCAGACCAGGGCGTAGTGGCTCGATTTGCGCCGTCCTGAAGGAAGATCTGCTCGGCTGGTGATCAAGACGTGTTTGGGGAGAGGCACAACGTTACCTCGCCCATCGACCCAACTGTTCCAGACAAAGATGTCTTCAGGGCGAACGTCAATGTCCTTCGGCTTGCTGAACATC  >  NC_002947/6155659‑6155954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑