Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	623,517	C→A	6.6%	R55L (CGG→CTG)	PP_0536 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	623,517	0	C	A	6.6%	227.6 / 5.8	76	R55L (CGG→CTG)	PP_0536	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (33/37);  new base A (2/3);  total (35/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.34e-01

CGTTGACTGCTCCAACATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTT  >  NC_002947/623372‑623664                                                                                                                                                  |                                                                                                                                                    cGTTGACTGCTCCAACATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGct                                                                                                                                                  >  5:325405/1‑149 (MQ=255)   ttGACTGCTCCAACATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGctct                                                                                                                                                <  6:325405/149‑1 (MQ=255)      aCTGCTCCAACATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGc                                                                                                                                              <  3:151039/148‑1 (MQ=255)       cTGCTCCAACATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTTCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCa                                                                                                                                             <  8:152189/148‑1 (MQ=255)          cTCCAACATCTGGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCAATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCa                                                                                                                                          <  4:272953/148‑1 (MQ=255)           tCCAACATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCAGTTTCATGGTGGTGCCGCTCTGCATCAc                                                                                                                                         >  6:289310/1‑148 (MQ=255)            ccAACATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGATGCAGAGCGGCAccacca                                                                                                                                       >  2:205004/1‑149 (MQ=255)             cAACATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCAt                                                                                                                                      >  6:20451/1‑149 (MQ=255)             cAACATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGATGCAGAGCGGCAccacca                                                                                                                                       <  1:205004/148‑1 (MQ=255)             cAACATCCTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGATGCAGAGCGGCACCACCAt                                                                                                                                      <  7:354834/149‑1 (MQ=255)              aaCATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATg                                                                                                                                     >  1:370444/1‑149 (MQ=255)              aaCATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGATGCAGAGCGGCACCACCAt                                                                                                                                      <  5:223258/148‑1 (MQ=255)              aaCATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGATGCAGAGCGGCACCACCAt                                                                                                                                      >  6:223258/1‑148 (MQ=255)               aCATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATg                                                                                                                                     >  1:82603/1‑148 (MQ=255)                cATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGc                                                                                                                                    >  8:353768/1‑148 (MQ=255)                cATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGc                                                                                                                                    >  1:305863/1‑148 (MQ=255)                  tCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAg                                                                                                                                 >  7:137854/1‑149 (MQ=255)                      gAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAg                                                                                                                                 <  7:353768/145‑1 (MQ=255)                      gAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAg                                                                                                                                 <  7:208332/145‑1 (MQ=255)                       aGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTc                                                                                                                            <  5:20451/149‑1 (MQ=255)                            tCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGtt                                                                                                                       <  1:142731/149‑1 (MQ=255)                              cTGCAATTCGCGTGAAGGCTTTATGATCTTGCTAAATGGCCATGACACGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGATCTTCATTTTCATGGTGGTGACGATCAGCATCACCATGCAAGGCTACCAGTTGc                                                                                                                     >  6:14677/1‑149 (MQ=255)                               tGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCt                                                                                                                    >  2:64916/1‑149 (MQ=255)                                 cAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCt                                                                                                                    <  7:73157/147‑1 (MQ=255)                                    ttCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAaac                                                                                                               <  7:223003/149‑1 (MQ=255)                                    ttCGCGTGAAGGCTTTATGATCCTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAaac                                                                                                               <  7:117472/149‑1 (MQ=255)                                    ttCGCGTGAACGCTTTAACATCTTGCTCAATCCCCAAGACCCGTAGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAATCTCTACATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGTCTTCCAGATGCTTAaac                                                                                                               >  4:105088/1‑149 (MQ=255)                                         gTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAaacaac                                                                                                            <  2:82603/147‑1 (MQ=255)                                         gTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAg                                                                                                          >  2:290887/1‑149 (MQ=255)                                          tGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATg                                                                                                                                     >  8:346739/1‑121 (MQ=255)                                          tGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATg                                                                                                                                     <  7:346739/121‑1 (MQ=255)                                           gAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCt                                                                                                        >  6:370570/1‑149 (MQ=255)                                           gAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCt                                                                                                        >  4:100940/1‑149 (MQ=255)                                           gAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAACAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCt                                                                                                        >  3:103550/1‑149 (MQ=255)                                             aGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTg                                                                                                      <  3:366560/149‑1 (MQ=255)                                               gCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCTAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATACAAGGCTTCCAGTTGCTTAAACAACAGCTTGCa                                                                                                    <  5:94881/149‑1 (MQ=255)                                               gCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCa                                                                                                    <  1:64916/149‑1 (MQ=255)                                                 tttATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGt                                                                                                  >  1:335065/1‑149 (MQ=255)                                                       aTCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCg                                                                                             <  2:305863/148‑1 (MQ=255)                                                       aTCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTACAGTTGCTTAAACAACAGCTTCAAGTCATctg                                                                                            >  8:284068/1‑147 (MQ=255)                                                          ttGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCAc                                                                                         >  3:264144/1‑149 (MQ=255)                                                                aaTGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCg                                                                                      >  5:83162/1‑146 (MQ=255)                                                                    gCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATG‑AAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGcc                                                                               <  1:370721/148‑1 (MQ=255)                                                                       aTGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTTCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCgt                                                                             >  4:49362/1‑148 (MQ=255)                                                                       aTGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCgt                                                                             <  3:49362/148‑1 (MQ=255)                                                                          aCCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTc                                                                          <  1:290887/148‑1 (MQ=255)                                                                          aCCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCGCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTc                                                                          <  4:160672/148‑1 (MQ=255)                                                                           ccagTTGGTGAATCGGTGCGTCGGTTGTCAGCAATGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCtt                                                                        <  5:3979/146‑1 (MQ=255)                                                                                   tGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCAATTTCATGGTGGTGCCGCTCTGCCTCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCACAGACCCGTGTCTTATccaca                                                                 >  3:303092/1‑145 (MQ=255)                                                                                    gAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCtctc                                                                >  6:79484/1‑148 (MQ=255)                                                                                      aTCGGTGCGTCGGTTGTCAACAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAg                                                             <  4:103550/149‑1 (MQ=255)                                                                                       tCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAg                                                             >  7:14679/1‑148 (MQ=255)                                                                                             cGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCt                                                      >  2:134597/1‑149 (MQ=255)                                                                                                cGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCttt                                                    >  2:63048/1‑148 (MQ=255)                                                                                                     gTCAGCAAGGGCAGCCGAATAAGCTCTTCATTTTGATGGTGGTGCCGCTCTGCTTCACCATGCAAGTGTTCCAGTTGCTTTAAGAACAGCTTGCAGTCATCGGCACGCGCAGAGCCTTGTCTTATCCTCTCCAGTAATTCTTTTATCt                                               <  5:14677/148‑1 (MQ=255)                                                                                                     gTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCt                                               <  4:264144/148‑1 (MQ=255)                                                                                                      tCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATTGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGa                                             <  5:370570/149‑1 (MQ=255)                                                                                                        aGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCt                                               >  1:165785/1‑145 (MQ=255)                                                                                                        aGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCt                                               <  2:165785/145‑1 (MQ=255)                                                                                                        aGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAg                                            >  7:51939/1‑148 (MQ=255)                                                                                                         gCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGt                                           >  3:23338/1‑148 (MQ=255)                                                                                                                cAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCGGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTgg                                   <  7:284068/149‑1 (MQ=255)                                                                                                                 aGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGt                                  <  1:63048/149‑1 (MQ=255)                                                                                                                 aGCCGAATAAGCGCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGt                                  <  8:137854/149‑1 (MQ=255)                                                                                                                           gCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCa                        >  1:86763/1‑149 (MQ=255)                                                                                                                             tctTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGAACCCGCAGAGCCGTGTCTTATCATCTACAGTAATTCTTTGATCTGAGTAATTTTGGTATCCACCTCat                       >  6:308335/1‑148 (MQ=255)                                                                                                                               ttCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCatgat                    >  3:132799/1‑149 (MQ=255)                                                                                                                                 cATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCatga                     <  5:79484/146‑1 (MQ=255)                                                                                                                                   ttttCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCTACCTCATGATGATa                <  7:185919/149‑1 (MQ=255)                                                                                                                                     ttCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAg               <  5:289310/148‑1 (MQ=255)                                                                                                                                       cATGTTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACATCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGc             <  5:308335/148‑1 (MQ=255)                                                                                                                                        aTGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACTGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGa                                             <  1:229654/115‑1 (MQ=255)                                                                                                                                        aTGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGa                                             >  2:229654/1‑115 (MQ=255)                                                                                                                                           gtggtgCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGc         <  3:100940/148‑1 (MQ=255)                                                                                                                                              gtgCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGct     <  6:83162/149‑1 (MQ=255)                                                                                                                                               tgCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGctc    <  2:335065/149‑1 (MQ=255)                                                                                                                                                  cGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCtt  >  7:318793/1‑148 (MQ=255)                                                                                                                                                  |                                                                                                                                                    CGTTGACTGCTCCAACATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTT  >  NC_002947/623372‑623664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑