Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 623,520 T→G 7.6% E54A (GAG→GCG) ‡ PP_0536 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947623,5200TG 7.6% 223.6 / 7.1 75E54A (GAG→GCG) ‡PP_0536hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (33/36);  new base G (2/4);  total (35/40)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTTGACTGCTCCAACATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAA  >  NC_002947/623372‑623668
                                                                                                                                                    |                                                                                                                                                    
cGTTGACTGCTCCAACATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGct                                                                                                                                                      >  5:325405/1‑149 (MQ=255)
  ttGACTGCTCCAACATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGctct                                                                                                                                                    <  6:325405/149‑1 (MQ=255)
     aCTGCTCCAACATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGc                                                                                                                                                  <  3:151039/148‑1 (MQ=255)
      cTGCTCCAACATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTTCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCa                                                                                                                                                 <  8:152189/148‑1 (MQ=255)
         cTCCAACATCTGGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCAATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCa                                                                                                                                              <  4:272953/148‑1 (MQ=255)
          tCCAACATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCAGTTTCATGGTGGTGCCGCTCTGCATCAc                                                                                                                                             >  6:289310/1‑148 (MQ=255)
           ccAACATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGATGCAGAGCGGCAccacca                                                                                                                                           >  2:205004/1‑149 (MQ=255)
            cAACATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCAt                                                                                                                                          >  6:20451/1‑149 (MQ=255)
            cAACATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGATGCAGAGCGGCAccacca                                                                                                                                           <  1:205004/148‑1 (MQ=255)
            cAACATCCTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGATGCAGAGCGGCACCACCAt                                                                                                                                          <  7:354834/149‑1 (MQ=255)
             aaCATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATg                                                                                                                                         >  1:370444/1‑149 (MQ=255)
             aaCATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGATGCAGAGCGGCACCACCAt                                                                                                                                          <  5:223258/148‑1 (MQ=255)
             aaCATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGATGCAGAGCGGCACCACCAt                                                                                                                                          >  6:223258/1‑148 (MQ=255)
              aCATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATg                                                                                                                                         >  1:82603/1‑148 (MQ=255)
               cATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGc                                                                                                                                        >  8:353768/1‑148 (MQ=255)
               cATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGc                                                                                                                                        >  1:305863/1‑148 (MQ=255)
                 tCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAg                                                                                                                                     >  7:137854/1‑149 (MQ=255)
                     gAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAg                                                                                                                                     <  7:353768/145‑1 (MQ=255)
                     gAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAg                                                                                                                                     <  7:208332/145‑1 (MQ=255)
                      aGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTc                                                                                                                                <  5:20451/149‑1 (MQ=255)
                           tCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGtt                                                                                                                           <  1:142731/149‑1 (MQ=255)
                             cTGCAATTCGCGTGAAGGCTTTATGATCTTGCTAAATGGCCATGACACGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGATCTTCATTTTCATGGTGGTGACGATCAGCATCACCATGCAAGGCTACCAGTTGc                                                                                                                         >  6:14677/1‑149 (MQ=255)
                              tGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCt                                                                                                                        >  2:64916/1‑149 (MQ=255)
                                cAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCt                                                                                                                        <  7:73157/147‑1 (MQ=255)
                                   ttCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAaac                                                                                                                   <  7:223003/149‑1 (MQ=255)
                                   ttCGCGTGAAGGCTTTATGATCCTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAaac                                                                                                                   <  7:117472/149‑1 (MQ=255)
                                   ttCGCGTGAACGCTTTAACATCTTGCTCAATCCCCAAGACCCGTAGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAATCTCTACATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGTCTTCCAGATGCTTAaac                                                                                                                   >  4:105088/1‑149 (MQ=255)
                                        gTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAaacaac                                                                                                                <  2:82603/147‑1 (MQ=255)
                                        gTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAg                                                                                                              >  2:290887/1‑149 (MQ=255)
                                         tGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATg                                                                                                                                         >  8:346739/1‑121 (MQ=255)
                                         tGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATg                                                                                                                                         <  7:346739/121‑1 (MQ=255)
                                          gAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCt                                                                                                            >  4:100940/1‑149 (MQ=255)
                                          gAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCt                                                                                                            >  6:370570/1‑149 (MQ=255)
                                          gAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAACAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCt                                                                                                            >  3:103550/1‑149 (MQ=255)
                                            aGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTg                                                                                                          <  3:366560/149‑1 (MQ=255)
                                              gCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCTAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATACAAGGCTTCCAGTTGCTTAAACAACAGCTTGCa                                                                                                        <  5:94881/149‑1 (MQ=255)
                                              gCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCa                                                                                                        <  1:64916/149‑1 (MQ=255)
                                                tttATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGt                                                                                                      >  1:335065/1‑149 (MQ=255)
                                                      aTCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCg                                                                                                 <  2:305863/148‑1 (MQ=255)
                                                      aTCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTACAGTTGCTTAAACAACAGCTTCAAGTCATctg                                                                                                >  8:284068/1‑147 (MQ=255)
                                                         ttGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCAc                                                                                             >  3:264144/1‑149 (MQ=255)
                                                               aaTGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCg                                                                                          >  5:83162/1‑146 (MQ=255)
                                                                   gCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATG‑AAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGcc                                                                                   <  1:370721/148‑1 (MQ=255)
                                                                      aTGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTTCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCgt                                                                                 >  4:49362/1‑148 (MQ=255)
                                                                      aTGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCgt                                                                                 <  3:49362/148‑1 (MQ=255)
                                                                         aCCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTc                                                                              <  1:290887/148‑1 (MQ=255)
                                                                         aCCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCGCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTc                                                                              <  4:160672/148‑1 (MQ=255)
                                                                          ccagTTGGTGAATCGGTGCGTCGGTTGTCAGCAATGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCtt                                                                            <  5:3979/146‑1 (MQ=255)
                                                                                  tGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCAATTTCATGGTGGTGCCGCTCTGCCTCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCACAGACCCGTGTCTTATccaca                                                                     >  3:303092/1‑145 (MQ=255)
                                                                                   gAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCtctc                                                                    >  6:79484/1‑148 (MQ=255)
                                                                                     aTCGGTGCGTCGGTTGTCAACAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAg                                                                 <  4:103550/149‑1 (MQ=255)
                                                                                      tCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAg                                                                 >  7:14679/1‑148 (MQ=255)
                                                                                            cGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCt                                                          >  2:134597/1‑149 (MQ=255)
                                                                                               cGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCttt                                                        >  2:63048/1‑148 (MQ=255)
                                                                                                    gTCAGCAAGGGCAGCCGAATAAGCTCTTCATTTTGATGGTGGTGCCGCTCTGCTTCACCATGCAAGTGTTCCAGTTGCTTTAAGAACAGCTTGCAGTCATCGGCACGCGCAGAGCCTTGTCTTATCCTCTCCAGTAATTCTTTTATCt                                                   <  5:14677/148‑1 (MQ=255)
                                                                                                    gTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCt                                                   <  4:264144/148‑1 (MQ=255)
                                                                                                     tCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATTGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGa                                                 <  5:370570/149‑1 (MQ=255)
                                                                                                       aGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCt                                                   >  1:165785/1‑145 (MQ=255)
                                                                                                       aGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCt                                                   <  2:165785/145‑1 (MQ=255)
                                                                                                       aGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAg                                                >  7:51939/1‑148 (MQ=255)
                                                                                                        gCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGt                                               >  3:23338/1‑148 (MQ=255)
                                                                                                               cAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCGGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTgg                                       <  7:284068/149‑1 (MQ=255)
                                                                                                                aGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGt                                      <  1:63048/149‑1 (MQ=255)
                                                                                                                aGCCGAATAAGCGCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGt                                      <  8:137854/149‑1 (MQ=255)
                                                                                                                          gCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCa                            >  1:86763/1‑149 (MQ=255)
                                                                                                                            tctTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGAACCCGCAGAGCCGTGTCTTATCATCTACAGTAATTCTTTGATCTGAGTAATTTTGGTATCCACCTCat                           >  6:308335/1‑148 (MQ=255)
                                                                                                                              ttCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCatgat                        >  3:132799/1‑149 (MQ=255)
                                                                                                                                cATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCatga                         <  5:79484/146‑1 (MQ=255)
                                                                                                                                  ttttCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCTACCTCATGATGATa                    <  7:185919/149‑1 (MQ=255)
                                                                                                                                    ttCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAg                   <  5:289310/148‑1 (MQ=255)
                                                                                                                                      cATGTTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACATCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGc                 <  5:308335/148‑1 (MQ=255)
                                                                                                                                       aTGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACTGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGa                                                 <  1:229654/115‑1 (MQ=255)
                                                                                                                                       aTGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGa                                                 >  2:229654/1‑115 (MQ=255)
                                                                                                                                          gtggtgCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGc             <  3:100940/148‑1 (MQ=255)
                                                                                                                                             gtgCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGct         <  6:83162/149‑1 (MQ=255)
                                                                                                                                              tgCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGctc        <  2:335065/149‑1 (MQ=255)
                                                                                                                                                 cGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCtt      >  7:318793/1‑148 (MQ=255)
                                                                                                                                                    tctGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCaa  >  1:85737/1‑149 (MQ=255)
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CGTTGACTGCTCCAACATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAA  >  NC_002947/623372‑623668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑