Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 623,522 T→G 6.3% A53A (GCA→GCC)  PP_0536 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947623,5220TG 6.3% 234.4 / 5.0 75A53A (GCA→GCC) PP_0536hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (32/37);  new base G (2/3);  total (35/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.11e-01

TTGACTGCTCCAACATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAA  >  NC_002947/623374‑623668
                                                                                                                                                    |                                                                                                                                                  
ttGACTGCTCCAACATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGctct                                                                                                                                                    <  6:325405/149‑1 (MQ=255)
   aCTGCTCCAACATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGc                                                                                                                                                  <  3:151039/148‑1 (MQ=255)
    cTGCTCCAACATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTTCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCa                                                                                                                                                 <  8:152189/148‑1 (MQ=255)
       cTCCAACATCTGGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCAATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCa                                                                                                                                              <  4:272953/148‑1 (MQ=255)
        tCCAACATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCAGTTTCATGGTGGTGCCGCTCTGCATCAc                                                                                                                                             >  6:289310/1‑148 (MQ=255)
         ccAACATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGATGCAGAGCGGCAccacca                                                                                                                                           >  2:205004/1‑149 (MQ=255)
          cAACATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCAt                                                                                                                                          >  6:20451/1‑149 (MQ=255)
          cAACATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGATGCAGAGCGGCAccacca                                                                                                                                           <  1:205004/148‑1 (MQ=255)
          cAACATCCTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGATGCAGAGCGGCACCACCAt                                                                                                                                          <  7:354834/149‑1 (MQ=255)
           aaCATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATg                                                                                                                                         >  1:370444/1‑149 (MQ=255)
           aaCATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGATGCAGAGCGGCACCACCAt                                                                                                                                          >  6:223258/1‑148 (MQ=255)
           aaCATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGATGCAGAGCGGCACCACCAt                                                                                                                                          <  5:223258/148‑1 (MQ=255)
            aCATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATg                                                                                                                                         >  1:82603/1‑148 (MQ=255)
             cATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGc                                                                                                                                        >  8:353768/1‑148 (MQ=255)
             cATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGc                                                                                                                                        >  1:305863/1‑148 (MQ=255)
               tCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAg                                                                                                                                     >  7:137854/1‑149 (MQ=255)
                   gAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAg                                                                                                                                     <  7:208332/145‑1 (MQ=255)
                   gAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAg                                                                                                                                     <  7:353768/145‑1 (MQ=255)
                    aGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTc                                                                                                                                <  5:20451/149‑1 (MQ=255)
                         tCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGtt                                                                                                                           <  1:142731/149‑1 (MQ=255)
                           cTGCAATTCGCGTGAAGGCTTTATGATCTTGCTAAATGGCCATGACACGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGATCTTCATTTTCATGGTGGTGACGATCAGCATCACCATGCAAGGCTACCAGTTGc                                                                                                                         >  6:14677/1‑149 (MQ=255)
                            tGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCt                                                                                                                        >  2:64916/1‑149 (MQ=255)
                              cAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCt                                                                                                                        <  7:73157/147‑1 (MQ=255)
                                 ttCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAaac                                                                                                                   <  7:223003/149‑1 (MQ=255)
                                 ttCGCGTGAAGGCTTTATGATCCTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAaac                                                                                                                   <  7:117472/149‑1 (MQ=255)
                                 ttCGCGTGAACGCTTTAACATCTTGCTCAATCCCCAAGACCCGTAGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAATCTCTACATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGTCTTCCAGATGCTTAaac                                                                                                                   >  4:105088/1‑149 (MQ=255)
                                      gTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAaacaac                                                                                                                <  2:82603/147‑1 (MQ=255)
                                      gTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAg                                                                                                              >  2:290887/1‑149 (MQ=255)
                                       tGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATg                                                                                                                                         >  8:346739/1‑121 (MQ=255)
                                       tGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATg                                                                                                                                         <  7:346739/121‑1 (MQ=255)
                                        gAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCt                                                                                                            >  4:100940/1‑149 (MQ=255)
                                        gAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCt                                                                                                            >  6:370570/1‑149 (MQ=255)
                                        gAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAACAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCt                                                                                                            >  3:103550/1‑149 (MQ=255)
                                          aGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTg                                                                                                          <  3:366560/149‑1 (MQ=255)
                                            gCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCTAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATACAAGGCTTCCAGTTGCTTAAACAACAGCTTGCa                                                                                                        <  5:94881/149‑1 (MQ=255)
                                            gCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCa                                                                                                        <  1:64916/149‑1 (MQ=255)
                                              tttATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGt                                                                                                      >  1:335065/1‑149 (MQ=255)
                                                    aTCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCg                                                                                                 <  2:305863/148‑1 (MQ=255)
                                                    aTCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTACAGTTGCTTAAACAACAGCTTCAAGTCATctg                                                                                                >  8:284068/1‑147 (MQ=255)
                                                       ttGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCAc                                                                                             >  3:264144/1‑149 (MQ=255)
                                                             aaTGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCg                                                                                          >  5:83162/1‑146 (MQ=255)
                                                                 gCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATG‑AAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGcc                                                                                   <  1:370721/148‑1 (MQ=255)
                                                                    aTGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTTCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCgt                                                                                 >  4:49362/1‑148 (MQ=255)
                                                                    aTGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCgt                                                                                 <  3:49362/148‑1 (MQ=255)
                                                                       aCCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTc                                                                              <  1:290887/148‑1 (MQ=255)
                                                                       aCCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCGCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTc                                                                              <  4:160672/148‑1 (MQ=255)
                                                                        ccagTTGGTGAATCGGTGCGTCGGTTGTCAGCAATGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCtt                                                                            <  5:3979/146‑1 (MQ=255)
                                                                                tGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCAATTTCATGGTGGTGCCGCTCTGCCTCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCACAGACCCGTGTCTTATccaca                                                                     >  3:303092/1‑145 (MQ=255)
                                                                                 gAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCtctc                                                                    >  6:79484/1‑148 (MQ=255)
                                                                                   aTCGGTGCGTCGGTTGTCAACAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAg                                                                 <  4:103550/149‑1 (MQ=255)
                                                                                    tCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAg                                                                 >  7:14679/1‑148 (MQ=255)
                                                                                          cGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCt                                                          >  2:134597/1‑149 (MQ=255)
                                                                                             cGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCttt                                                        >  2:63048/1‑148 (MQ=255)
                                                                                                  gTCAGCAAGGGCAGCCGAATAAGCTCTTCATTTTGATGGTGGTGCCGCTCTGCTTCACCATGCAAGTGTTCCAGTTGCTTTAAGAACAGCTTGCAGTCATCGGCACGCGCAGAGCCTTGTCTTATCCTCTCCAGTAATTCTTTTATCt                                                   <  5:14677/148‑1 (MQ=255)
                                                                                                  gTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCt                                                   <  4:264144/148‑1 (MQ=255)
                                                                                                   tCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATTGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGa                                                 <  5:370570/149‑1 (MQ=255)
                                                                                                     aGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCt                                                   <  2:165785/145‑1 (MQ=255)
                                                                                                     aGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCt                                                   >  1:165785/1‑145 (MQ=255)
                                                                                                     aGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAg                                                >  7:51939/1‑148 (MQ=255)
                                                                                                      gCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGt                                               >  3:23338/1‑148 (MQ=255)
                                                                                                             cAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCGGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTgg                                       <  7:284068/149‑1 (MQ=255)
                                                                                                              aGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGt                                      <  1:63048/149‑1 (MQ=255)
                                                                                                              aGCCGAATAAGCGCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGt                                      <  8:137854/149‑1 (MQ=255)
                                                                                                                        gCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCa                            >  1:86763/1‑149 (MQ=255)
                                                                                                                          tctTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGAACCCGCAGAGCCGTGTCTTATCATCTACAGTAATTCTTTGATCTGAGTAATTTTGGTATCCACCTCat                           >  6:308335/1‑148 (MQ=255)
                                                                                                                            ttCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCatgat                        >  3:132799/1‑149 (MQ=255)
                                                                                                                              cATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCatga                         <  5:79484/146‑1 (MQ=255)
                                                                                                                                ttttCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCTACCTCATGATGATa                    <  7:185919/149‑1 (MQ=255)
                                                                                                                                  ttCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAg                   <  5:289310/148‑1 (MQ=255)
                                                                                                                                    cATGTTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACATCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGc                 <  5:308335/148‑1 (MQ=255)
                                                                                                                                     aTGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACTGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGa                                                 <  1:229654/115‑1 (MQ=255)
                                                                                                                                     aTGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGa                                                 >  2:229654/1‑115 (MQ=255)
                                                                                                                                        gtggtgCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGc             <  3:100940/148‑1 (MQ=255)
                                                                                                                                           gtgCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGct         <  6:83162/149‑1 (MQ=255)
                                                                                                                                            tgCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGctc        <  2:335065/149‑1 (MQ=255)
                                                                                                                                               cGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCtt      >  7:318793/1‑148 (MQ=255)
                                                                                                                                                  tctGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCaa  >  1:85737/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TTGACTGCTCCAACATCTTGAGCTGTCCTGCAATTCGCGTGAAGGCTTTATGATCTTGCTCAATGGCCATGACCCGTTGGTGAATCGGTGCGTCGGTTGTCAGCAAGGCCAGCCGAATAAGCTCTTCATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAA  >  NC_002947/623374‑623668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑