Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	648,289	A→T	11.3%	intergenic (+58/+33)	acoR → / ← accC	acetoin catabolism regulatory protein/acetyl‑CoA carboxylase biotin carboxylase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	648,289	0	A	T	11.3%	140.0 / 8.8	53	intergenic (+58/+33)	acoR/accC	acetoin catabolism regulatory protein/acetyl‑CoA carboxylase biotin carboxylase subunit
Reads supporting (aligned to +/- strand):  ref base A (18/29);  new base T (2/4);  total (20/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01

TGCGGCACGGGCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCC‑GGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTGGTCTTGATG  >  NC_002947/648144‑648430                                                                                                                                                  |                                                                                                                                               tGCGGCACGGGCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAgg                                                                                                                                              <  5:30663/148‑1 (MQ=255)      cACGGGCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCC‑ggg                                                                                                                                       <  2:144894/149‑1 (MQ=255)         gggCTTTGGGCATTGCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGACCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCCCTGTGCC‑GGGGtt                                                                                                                                    <  3:52220/149‑1 (MQ=255)           gCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCTCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCCCTGTGCC‑GGGGtttt                                                                                                                                  <  8:77345/149‑3 (MQ=255)               tGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCC‑GGGGTTCTGGt                                                                                                                               <  4:37374/148‑1 (MQ=255)               tGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCC‑GGGGTTCTGGt                                                                                                                               <  8:209563/148‑1 (MQ=255)                gggCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCCCTGTGCC‑GGGGttttttat                                                                                                                              <  2:111937/148‑7 (MQ=255)                 ggCATTTCGCGGGCGCCGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGCGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCC‑GGGGTTCTGGTACa                                                                                                                            <  8:227124/149‑1 (MQ=255)                 ggCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCC‑GGGGTTCTGGTAc                                                                                                                             <  5:296173/148‑1 (MQ=255)                      ttCGCGGGGGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGTTGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCC‑GGGGTTCTGGTACATcac                                                                                                                        <  4:314713/148‑1 (MQ=255)                           gggCGACGCTTGATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCC‑GGGGTTCTGGTACATCACACTaca                                                                                                                  <  7:170098/149‑1 (MQ=255)                                          ggCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCC‑GGGGTTCTGGt                                                                                                                               <  5:327286/121‑1 (MQ=255)                                              aGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCC‑GGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGcc                                                                                               <  3:199184/149‑1 (MQ=255)                                                   aTGAGCACGGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCC‑GGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGttt                                                                                          <  2:89997/149‑1 (MQ=255)                                                   aTGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCC‑GGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTg                                                                                                  <  7:188287/141‑1 (MQ=255)                                                      aGCACCGCATCGACCTGCCGCGTATGAAGGGGTAGCGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGTGCC‑GTGGTTCTTGTACATCAAACTACACGATCACTCCTGGTTGGCCAGTTtgt                                                                                        <  2:343800/148‑1 (MQ=255)                                                       gCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCC‑GGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGc                                                                                      <  1:274353/149‑1 (MQ=255)                                                           cGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCC‑GGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGa                                                                                  <  7:301067/149‑1 (MQ=255)                                                                       ccGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCCGATTCTGGGAGGGAATAAAAAACCCCCGCCCAGGGGC‑GGGGGTCTGGTACATCACACTACACGATCACTCCTGGTTGGGCAGTTTGTGGTCGAGGTAGTGAATGt                                                                      >  3:284921/1‑149 (MQ=255)                                                                        cgcgGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCC‑GGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGtt                                                                     <  2:330753/149‑1 (MQ=255)                                                                         gcgGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCC‑GGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTg                                                                    <  7:187522/149‑1 (MQ=255)                                                                         gcgGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCC‑GGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTg                                                                    <  5:9665/149‑1 (MQ=255)                                                                                  ggggTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCC‑GGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCt                                                           <  4:284921/149‑1 (MQ=255)                                                                                     gTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCC‑GGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTg                                                        <  5:256652/149‑1 (MQ=255)                                                                                      tcgcgCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCC‑GGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGc                                                       <  8:328437/145‑1 (MQ=255)                                                                                           cccTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCC‑GGGGTTCTGGTACATCACACTACAAGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAg                                                  >  8:249537/1‑149 (MQ=255)                                                                                            tcTGGGGCCGCGTCGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCC‑GGGGTTCTGGTACATCACACTCCACGATCACTCCTGGTTGGCCAG‑TTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGcc                                                >  5:188273/2‑149 (MQ=255)                                                                                                gggCCGCGTCGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCC‑GGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCtt                                              >  7:159700/1‑148 (MQ=255)                                                                                                 ggCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCC‑GGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTtca                                            <  3:202677/149‑1 (MQ=255)                                                                                                  gCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCC‑GGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCtt                                              <  5:105879/146‑1 (MQ=255)                                                                                                  gCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCC‑GGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCtt                                              >  6:105879/1‑146 (MQ=255)                                                                                                  gCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCC‑GGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTtc                                             >  8:326162/1‑147 (MQ=255)                                                                                                  gCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCC‑GGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTtc                                             <  7:326162/147‑1 (MQ=255)                                                                                                    cgcgACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCC‑GGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTtcatca                                         >  2:351040/1‑149 (MQ=255)                                                                                                            ggCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCC‑GGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAg                                 <  4:150251/149‑1 (MQ=255)                                                                                                              ccccAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCC‑GGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGaa                                                   <  5:281908/129‑1 (MQ=255)                                                                                                              ccccAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCC‑GGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGaa                                                   >  6:281908/1‑129 (MQ=255)                                                                                                                ccAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCC‑GGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTcg                             >  1:173068/1‑149 (MQ=255)                                                                                                                   gATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCC‑GGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCgg                          <  1:351040/149‑1 (MQ=255)                                                                                                                     ttCTGGCAGGCAATAAAAAACCCCGGCACAGGGCC‑GGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGt                         <  5:1337/148‑1 (MQ=255)                                                                                                                            aggcaATAAAAAACCCCGGCACAGGGCCTGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCgg                  >  1:214311/1‑149 (MQ=255)                                                                                                                              gcaATAAAAAACCCCGGCACAGGGCC‑GGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGAt               >  2:118804/1‑149 (MQ=255)                                                                                                                              gcaATAAAAAACCCCGGCACAGGGCC‑GGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGAt               >  6:183174/1‑149 (MQ=255)                                                                                                                              gcaATAAAAAACCCCGGCACAGGGCC‑GGGGTTCTGGAACATAACAGAACACGATCACTCCAGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGGTGAGCAGGGGGAt               >  3:333237/1‑149 (MQ=255)                                                                                                                                aaTAAAAAACCCCGGCACAGGGCC‑GGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGt             >  3:74035/1‑149 (MQ=255)                                                                                                                                aaTAAAAAACCCCGGCACAGGGCC‑GGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGt             >  5:60649/1‑149 (MQ=255)                                                                                                                                  tAAAAAACCCCGGCACAGGGCC‑GGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTg           >  8:64479/1‑149 (MQ=255)                                                                                                                                   ccagaaCCCCGGCCCTGTGCC‑GGGGTTTTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATg              <  3:157948/141‑1 (MQ=255)                                                                                                                                   ccagaaCCCCGGCCCTGTGCC‑GGGGTTTTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATg              >  4:157948/5‑145 (MQ=255)                                                                                                                                   aaaaaaCCCCGGCACAGGGCC‑GGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTgg          >  3:360208/1‑149 (MQ=255)                                                                                                                                     agaaCCCCGGCCCTGTGCC‑GGGGTTTTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTGGTc        >  7:111356/3‑149 (MQ=255)                                                                                                                                         ccccGGCACAGGGCC‑GGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACTCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTGGTCTTGa    <  7:249537/149‑1 (MQ=255)                                                                                                                                           ccGGCACAGGGCC‑GGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTGGTCTTGATg  >  8:325478/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                               TGCGGCACGGGCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCC‑GGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTGGTCTTGATG  >  NC_002947/648144‑648430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑