| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 650,846 | T→A | 6.8% | intergenic (‑135/+45) | aroQ‑I ← / ← dsbD‑I | type II 3‑dehydroquinate dehydratase/thiol/disulfide interchange protein 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 650,846 | 0 | T | A | 6.8% | 172.3 / 2.7 | 59 | intergenic (‑135/+45) | aroQ‑I/dsbD‑I | type II 3‑dehydroquinate dehydratase/thiol/disulfide interchange protein 1 |
| Reads supporting (aligned to +/- strand): ref base T (29/26); new base A (2/2); total (31/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.42e-01 | |||||||||||
GCGTTGCCATCTGCGGATTCCTTGGATTTGTAGGGCAATTCGACACAGCGCGGCGAGTGTGCCGTAAAGCAACGGCAAGTGTCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCGGCATTTATCTCGCCAATCACCCGATCAGCGGTAATTTCGCTGCCGTTCG > NC_002947/650702‑650991 | gCGTTGCCATCTGCGGATTCCTTGGATTTGTAGGGCAATTCGACACAGCGCGGCGAGTGTGCCGTAAAGCAACGGCAAGTGTCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAAt < 2:313369/149‑1 (MQ=255) ttGCCATCTGCGGATTCCTTGGATTTGTAGGGCAATTCGACACAGCGCGGCGAGTGTGCCGTAAAGCAACGGCAAGTGTCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCg < 1:204048/149‑1 (MQ=255) ccATCTGCGGATTCCTTGGATTTGTAGGGCAATTCGACACAGCGCGGCGAGTGTGCCGTAAAGCAACGGCAAGTGTCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATTTAAATATCCGGTc < 8:59712/149‑1 (MQ=255) ccATCTGCGGATTCCTTGGATTTGTAGGGCAATTCGACACAGCGCGGCGAGTGTGCCGTAAAGCAACGGCAAGTGTCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGt < 6:378388/148‑1 (MQ=255) tGCGGATTCCTTGGATTTGTAGGGCAATTCGACACAGCGCGGCGAGTGTGCCGTAAAGCAACGGCAAGTGTCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTTAATACGGTCACtaa > 8:202012/1‑147 (MQ=255) cGGATTCCTTGGATTTGTAGGGCAATTCGACACAGCGCGGCGAGTGTGCCGTAAAGCAACGGCAAGTGTCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCAtt < 8:195102/149‑1 (MQ=255) ccTTGGATTTGTAGGGCAATTCGACACAGCGCGGCGAGTGTGCCGTAAAGCAACGGCAAGTGTCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGGTCGCGCGAAGTATGTGTCCGGATATTTAAATCCGGTCACTCATTTCAg < 1:35689/147‑1 (MQ=255) cTTGGATTTGTAGGGCAATTCGACACAGCGCGGCGAGTGTGCCGTAAAGCAACGGCAAGTGTCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGa > 8:197809/1‑148 (MQ=255) cTTGGATTTGTAGGGCAATTCGACACAGCGCGGCGAGTGTGCCGTAAAGCAACGGCAAGTGTCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGAt > 7:52065/1‑149 (MQ=255) cTTGGATTTGTAGGGCAATTCGACACAGCGCGGCGAGTGTGCCGTAAAGCAACGGCAAGTGTCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGAt > 2:43964/1‑149 (MQ=255) cTTGGATTTGTAGGGCAATTCGACACAGCGCGGCGAGTGTGCCGTAAAGCAACGGCAAGTGCCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGa < 7:197809/148‑1 (MQ=255) ggATTTGTAGGGCAATTCGACACAGCGCGGCGAGTGTGCCGTAAAGCAACGGCAAGTGTCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGAtttt > 5:19979/1‑149 (MQ=255) gggCAATTCGACACAGCGCGGCGAGTGTGCCGTAAAGCAACGGCAAGTGTCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTc > 8:311969/1‑149 (MQ=255) ggCAATTCGACACAGCGCGGCGAGTGTGCCGTAAAGCAACGGCAAGTGTCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTcg > 8:281814/1‑149 (MQ=255) ggCAATTCGACACAGCGCGGCGAGTGTGCCGTAAAGCAACGGCAAGTGTCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTcg > 5:105092/1‑149 (MQ=255) ttCGACACAGCGCGGCGAGTGTGCCGTAAAGCAACGGCAAGTGTCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGa < 7:202012/149‑1 (MQ=255) cGACACAGCGCGGCGAGTGTGCCGTAAAGCAACGGCAAGTGTCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCTTTTCAGGATTTTTCCGAGCTCGCGGGaa < 1:209667/148‑1 (MQ=255) gcgcGGCGAGTGTGCCGTAAAGCAACGGCAAGTGTCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGa > 7:15484/1‑149 (MQ=255) cgcgGCGAGTGTGCCGTAAAGCAACGGCAAGTGTCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGAc < 6:105092/149‑1 (MQ=255) ggcgAGTGTGCCGTAAAGCAACGGCAAGTGTCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGa > 7:108289/1‑149 (MQ=255) aGTGTGCCGTAAAGCAACGGCAAGTGTCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACttt > 1:83932/1‑149 (MQ=255) tgCCGTAAAGCAACGGCAAGTGTCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCcg > 4:354354/1‑149 (MQ=255) cGTAAAGCAACGGCAAGTGTCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCAGTTCAGGAGTTTTCGGAGCTCGCGGGATGGTATTGACCGCCTTTGCcgag > 8:347159/1‑146 (MQ=255) aaGCAACGGCAAGTGTCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCAcgc < 2:83932/149‑1 (MQ=255) aGCAACGGCAAGTGTCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCAcgcg > 2:2426/1‑149 (MQ=255) tttaCGGCAAGTGTCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCAcgcgc < 4:188153/146‑1 (MQ=255) aaCGGCAAGTGTCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGt > 8:199898/1‑149 (MQ=255) cAAGTGTCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGaa > 8:318001/1‑149 (MQ=255) tCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTgc < 8:52065/148‑1 (MQ=255) cAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTgcgc < 7:281814/148‑1 (MQ=255) ttCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTgc < 8:177187/143‑1 (MQ=255) ttCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTgc > 7:177187/1‑143 (MQ=255) ccGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCa > 8:66588/1‑149 (MQ=255) ggCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCAcc < 1:2426/149‑1 (MQ=255) gCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCg < 7:199898/149‑1 (MQ=255) cAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTcc < 2:241161/82‑1 (MQ=255) cAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTcc > 1:241161/1‑82 (MQ=255) aGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCGGCAtt < 8:15484/149‑1 (MQ=255) aGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCGGCATTTAtc > 7:322211/1‑149 (MQ=255) gCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCGGCATTTAtct < 8:29246/149‑1 (MQ=255) cACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCGGCATTTAtctc < 6:153304/149‑1 (MQ=255) cGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCGGCATTTATCTCGc < 1:43964/149‑1 (MQ=255) tGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCGGCATTTATCTCGCCaa < 6:19979/149‑1 (MQ=255) gTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCAc > 7:36014/1‑130 (MQ=255) gTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCAc < 8:36014/130‑1 (MQ=255) tCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCGGCATTTATCTCGCCAAt > 1:75955/1‑148 (MQ=255) tCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCGGCATTTATCTCGCCAAt > 5:320333/1‑148 (MQ=255) cgcgAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCGGCATTTATCTCGCCAATCAcc < 4:314120/141‑1 (MQ=255) cgcgAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCGGCATTTATCTCGCCAATCAcc > 3:314120/1‑141 (MQ=255) cgcgAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCGGCATTTATCTCGCCAATCACCCGATCAGc > 2:177067/1‑149 (MQ=255) aaGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCGGCATTTATCTCGCCAATCACCCGATCAGCGGTa < 8:108289/149‑1 (MQ=255) tgtgACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCGGCATTTATCTCGCCAATCACCCGATCAGCGGTAATTTc > 6:99186/1‑149 (MQ=255) tgtgACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCGGCATTTATCTCGCCAATCACCCGATCAGCGGTAATTTc > 3:3649/1‑149 (MQ=255) aCCGGATTTAAATATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCGGCAt > 1:136575/1‑111 (MQ=255) aCCGGATTTAAATATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCGGCAt < 2:136575/111‑1 (MQ=255) aCCGGATTTAAATATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCGGCATTTATCTCGCCAATCACCCGATCAGCGGTAATTTCGCTg > 8:108498/1‑149 (MQ=255) cGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCGGCATTTATCTCg > 2:137038/1‑117 (MQ=255) cGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCGGCATTTATCTCg < 1:137038/117‑1 (MQ=255) gATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCGGCATTTATCTCGCCAATCACCCGATCAGCGGTAATTTCGCTGCCGt > 6:158768/1‑149 (MQ=255) ttAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCGGCATTTATCTCGCCAATCACCCGATCAGCGGTAATTTCGCTGCCGTTCg > 3:313929/1‑147 (MQ=255) | GCGTTGCCATCTGCGGATTCCTTGGATTTGTAGGGCAATTCGACACAGCGCGGCGAGTGTGCCGTAAAGCAACGGCAAGTGTCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCGGCATTTATCTCGCCAATCACCCGATCAGCGGTAATTTCGCTGCCGTTCG > NC_002947/650702‑650991 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |